tnfsf11 rankl picokine elisa kit  (Boster Bio)

Journal: MedComm

Article Title: Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling

doi: 10.1002/mco2.131

Figure Lengend Snippet: Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

Article Snippet: ATP production was quantified using a CellTiter‐Glo Luminescent Cell Viability Assay Kit (Promega, USA)., Serum levels of OPG and RANKL were measured by mouse OPG ELISA Kit (Boster Biological Technology) and TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) according to the manufacturer's instructions.

Techniques: Mouse Assay, Staining, Isolation, Cell Culture, Incubation, Micro-CT

Journal: Scientific Reports

Article Title: Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson’s disease model mice

doi: 10.1038/s41598-019-50336-4

Figure Lengend Snippet: Dopaminergic neurons suppress osteoclastogenesis. ( A ) The percentage of osteoclast precursor cells, characterized by the expression of the cell surface markers c-kit and c-fms, together with the absence or dull expression of CD11b, in bone marrow of control and MPTP-injected mice. Representative data (left) and quantification (right, control, n = 8; MPTP, n = 8) are shown. ( B ) Osteoclast differentiation of BMMs obtained from control and MPTP-injected mice. Left: representative images obtained from more than three independent experiments. Right: quantification of TRAP + multinuclear osteoclasts (MNC) (control, n = 6; MPTP, n = 6). ( C ) mRNA expression of NFATc1 of cells obtained from control and MPTP-injected mice 2 days after RANKL stimulation (control, n = 8; MPTP, n = 8). ( D ) Osteoclast differentiation in presence of 2% serum obtained from control and MPTP-injected mice and the effect of anti-prolactin neutralizing antibody in the presence of MPTP serum (left). Left: representative images obtained more than three independent experiments. Right: quantification of TRAP + MNC (control, n = 6; MPTP, n = 6; MPTP+anti-prolactin Ab, n = 6). ( E ) mRNA expression of NFATc1 of cells 2 days after RANKL stimulation in the presence of mouse serum (control, n = 8; MPTP, n = 8). ( F ) Ratio of RANKL/OPG in serum from control and MPTP-injected mice (control, n = 8; MPTP, n = 8). Statistical analyses were performed using Student’s t-test ( A – C , E , F ) or ANOVA with Dunnett’s multiple-comparison test ( D ). * P

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The concentrations of RANKL, OPG and Hcy in obtained mouse serum were determined using a Mouse TNFSF11/RANKL EZ-Set™ ELISA Kit, Mouse OPG (TNFRSF11B) PicoKine™ ELISA Kit (Boster Biological Technology) and Hcy ELISA Kit (Cosmo Bio), respectively, according to the individual manufacturer’s instructions.

Techniques: Expressing, Injection, Mouse Assay

Journal: PLoS ONE

Article Title: Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

doi: 10.1371/journal.pone.0132633

Figure Lengend Snippet: OPG/RANKL ratio in OBs/OCLs co-culture. In the graph is reported the ratio of the soluble factors measured by ELISA kit. Mean and standard error of three replicates are shown. Statistical significant differences among the samples are indicated in the graph: *p≤0.05.

Article Snippet: RANKL was detected by using Mouse OPG ELISA Kit and Mouse TNFSF11/RANKL ELISA Kit (Boster Biological Technology, Fremont, CA).

Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay