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    Boster Bio human tnfsf11 rankl elisa kit picokine
    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human <t>RANKL</t> ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
    Human Tnfsf11 Rankl Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tnfsf11 rankl elisa kit picokine/product/Boster Bio
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human tnfsf11 rankl elisa kit picokine - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation"

    Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-03484-5

    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
    Figure Legend Snippet: Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Techniques Used: Staining, Viability Assay, Enzyme-linked Immunosorbent Assay, Expressing

    Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
    Figure Legend Snippet: Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Techniques Used: Expressing, Polymerase Chain Reaction

    2) Product Images from "IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism"

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    Journal: Redox Biology

    doi: 10.1016/j.redox.2022.102373

    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P
    Figure Legend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Techniques Used: Expressing, Staining, Mouse Assay, Quantitative RT-PCR, Micro-CT

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    Boster Bio human tnfsf11 rankl elisa kit picokine
    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human <t>RANKL</t> ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
    Human Tnfsf11 Rankl Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tnfsf11 rankl elisa kit picokine/product/Boster Bio
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human tnfsf11 rankl elisa kit picokine - by Bioz Stars, 2022-09
    94/100 stars
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    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Journal: Scientific Reports

    Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation

    doi: 10.1038/s41598-021-03484-5

    Figure Lengend Snippet: Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Article Snippet: Total MMP-2 Quantikine ELISA Kit (MMP200, R & D Systems, Abingdon, UK), Human MMP-9 Quantikine ELISA Kit (DMP900, R & D Systems, Abingdon, UK) and Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842, BosterBio, CA, USA) were used based on each of the manufacturer’s protocol.

    Techniques: Staining, Viability Assay, Enzyme-linked Immunosorbent Assay, Expressing

    Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Journal: Scientific Reports

    Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation

    doi: 10.1038/s41598-021-03484-5

    Figure Lengend Snippet: Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Article Snippet: Total MMP-2 Quantikine ELISA Kit (MMP200, R & D Systems, Abingdon, UK), Human MMP-9 Quantikine ELISA Kit (DMP900, R & D Systems, Abingdon, UK) and Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842, BosterBio, CA, USA) were used based on each of the manufacturer’s protocol.

    Techniques: Expressing, Polymerase Chain Reaction

    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Journal: Redox Biology

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    doi: 10.1016/j.redox.2022.102373

    Figure Lengend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Article Snippet: Serum levels of OCN, OPG, RANKL and insulin were measured by mouse Osteocalcin ELISA Kit (BioVision), OPG ELISA Kit (Boster Biological Technology), TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) and Ultra-Sensitive Insulin ELISA Kit (Crystal Chem), respectively, according to the manufacturer's instructions.

    Techniques: Expressing, Staining, Mouse Assay, Quantitative RT-PCR, Micro-CT