il 6 elisa kits  (Boster Bio)


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    Boster Bio il 6 elisa kits
    Effects of HB on LPS-induced production of <t>IL-6</t> ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by <t>ELISA</t> kits. The values are presented as means ± SD of three independent experiments. * p
    Il 6 Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 6 elisa kits/product/Boster Bio
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    il 6 elisa kits - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "Heterophyllin B Ameliorates Lipopolysaccharide-Induced Inflammation and Oxidative Stress in RAW 264.7 Macrophages by Suppressing the PI3K/Akt Pathways"

    Article Title: Heterophyllin B Ameliorates Lipopolysaccharide-Induced Inflammation and Oxidative Stress in RAW 264.7 Macrophages by Suppressing the PI3K/Akt Pathways

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23040717

    Effects of HB on LPS-induced production of IL-6 ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by ELISA kits. The values are presented as means ± SD of three independent experiments. * p
    Figure Legend Snippet: Effects of HB on LPS-induced production of IL-6 ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by ELISA kits. The values are presented as means ± SD of three independent experiments. * p

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay

    2) Product Images from "LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs"

    Article Title: LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

    Journal: Open Medicine

    doi: 10.1515/med-2021-0200

    Knockdown of XIST weakened ox-LDL-induced effects on HUVECs. (a) The expression level of XIST in HUVECs treated with ox-LDL was determined by qPCR analysis. (b) The interference efficiency of si-XIST in HUVECs was assessed by qPCR assay. (c–f) HUVECs were divided into two groups: ox-LDL + si-NC and ox-LDL + si-XIST groups. (c) The proliferation capability of HUVECs was measured using MTT assay. (d) The flow cytometry assay was recruited to monitor the apoptosis of HUVECs. (e) The western blot analysis was applied to assess Bax and Bcl-2 expression in HUVECs. (f) A commercial available kit was used to assess LDH release in HUVECs supernatants. (g) The expression levels of IL-6, IL-1β, and TNF-α in ox-LDL-induced HUVECs were displayed, with 0 μg/mL of ox-LDL group as control. (h and i) QPCR and western blot analyses were employed to quantify mRNA and protein levels of α-SMA and SM22-α in HUVECs, respectively. Data shown are mean ± SD and from three independent experiments. * P
    Figure Legend Snippet: Knockdown of XIST weakened ox-LDL-induced effects on HUVECs. (a) The expression level of XIST in HUVECs treated with ox-LDL was determined by qPCR analysis. (b) The interference efficiency of si-XIST in HUVECs was assessed by qPCR assay. (c–f) HUVECs were divided into two groups: ox-LDL + si-NC and ox-LDL + si-XIST groups. (c) The proliferation capability of HUVECs was measured using MTT assay. (d) The flow cytometry assay was recruited to monitor the apoptosis of HUVECs. (e) The western blot analysis was applied to assess Bax and Bcl-2 expression in HUVECs. (f) A commercial available kit was used to assess LDH release in HUVECs supernatants. (g) The expression levels of IL-6, IL-1β, and TNF-α in ox-LDL-induced HUVECs were displayed, with 0 μg/mL of ox-LDL group as control. (h and i) QPCR and western blot analyses were employed to quantify mRNA and protein levels of α-SMA and SM22-α in HUVECs, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Flow Cytometry, Western Blot

    Ox-LDL suppressed proliferation and enhanced apoptosis and inflammatory response in HUVECs. (a and b) The cell viability of HUVECs exposed to ox-LDL was assessed by MTT assay. (c) The flow cytometry assay was performed for examining the apoptosis rate of HUVECs treated with 40 μg/mL of ox-LDL for 48 h. (d) The protein expression levels of Bax and Bcl-2 were measured by western blot assay. (e) LDH release in the medium was examined by a commercial kit. (f) The levels of IL-6, IL-1β, and TNF-α were evaluated in the medium by matched kits. (g and h) The qPCR and western blot assays were used to show the expression levels of α-SMA and SM22-α in HUVECs treated with ox-LDL. Data shown are mean ± SD and from three independent experiments. * P
    Figure Legend Snippet: Ox-LDL suppressed proliferation and enhanced apoptosis and inflammatory response in HUVECs. (a and b) The cell viability of HUVECs exposed to ox-LDL was assessed by MTT assay. (c) The flow cytometry assay was performed for examining the apoptosis rate of HUVECs treated with 40 μg/mL of ox-LDL for 48 h. (d) The protein expression levels of Bax and Bcl-2 were measured by western blot assay. (e) LDH release in the medium was examined by a commercial kit. (f) The levels of IL-6, IL-1β, and TNF-α were evaluated in the medium by matched kits. (g and h) The qPCR and western blot assays were used to show the expression levels of α-SMA and SM22-α in HUVECs treated with ox-LDL. Data shown are mean ± SD and from three independent experiments. * P

    Techniques Used: MTT Assay, Flow Cytometry, Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Ox-LDL repressed proliferation and induced apoptosis and inflammatory response in HUVECs by targeting the XIST/miR-98-5p axis. (a) QPCR was enforced to confirm the overexpression efficiency of miR-98-5p in HUVECs. (b–i) HUVECs were treated with ox-LDL + miR-NC, ox-LDL + miR-98-5p, ox-LDL + miR-98-5p + pcDNA, or ox-LDL + miR-98-5p + pcDNA-XIST. (b) MTT assay was used to assess cell viability of HUVECs. (c) Cells apoptosis assay was performed in transfected HUVECs by flow cytometry assay. (d and e) The protein expression levels of Bax and Bcl-2 were calculated in HUVECs by western blot assay. (f) The LDH release was displayed in the different groups by kit assay. (g) The concentrations of IL-6, IL-1β, and TNF-α in the medium were presented by ELISA assay. (h and i) The mRNA and protein expression levels of α-SMA and SM22-α in HUVECs were detected by qPCR and western blot assays, respectively. Data shown are mean ± SD and from three independent experiments. * P
    Figure Legend Snippet: Ox-LDL repressed proliferation and induced apoptosis and inflammatory response in HUVECs by targeting the XIST/miR-98-5p axis. (a) QPCR was enforced to confirm the overexpression efficiency of miR-98-5p in HUVECs. (b–i) HUVECs were treated with ox-LDL + miR-NC, ox-LDL + miR-98-5p, ox-LDL + miR-98-5p + pcDNA, or ox-LDL + miR-98-5p + pcDNA-XIST. (b) MTT assay was used to assess cell viability of HUVECs. (c) Cells apoptosis assay was performed in transfected HUVECs by flow cytometry assay. (d and e) The protein expression levels of Bax and Bcl-2 were calculated in HUVECs by western blot assay. (f) The LDH release was displayed in the different groups by kit assay. (g) The concentrations of IL-6, IL-1β, and TNF-α in the medium were presented by ELISA assay. (h and i) The mRNA and protein expression levels of α-SMA and SM22-α in HUVECs were detected by qPCR and western blot assays, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Techniques Used: Real-time Polymerase Chain Reaction, Over Expression, MTT Assay, Apoptosis Assay, Transfection, Flow Cytometry, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Knockdown of miR-98-5p reversed si-PAPPA induced the effect on proliferation, apoptosis, and inflammatory response in HUVECs. (a and b) The interference efficiency of si-PAPPA in HUVECs was measured using qPCR and western blot assays. (c–f) HUVECs were treated with ox-LDL + si-NC, ox-LDL + si-PAPPA, ox-LDL + si-PAPPA + anti-NC, or ox-LDL + si-PAPPA + anti-miR-98-5p. (c) The cell viability of HUVECs was estimated by MTT assay. (d) Apoptosis analysis was performed in HUVECs using flow cytometry assay. (e) The western blot assay was carried out to analyze Bax and Bcl-2 expression in HUVECs. (f) The LDH detection kit was used to test LDH release in HUVECs. (g) ELISA assay was applied to measure the levels of IL-6, IL-1β, and TNF-α in the medium, with 0 μg/mL of ox-LDL group as control. (h and i) The expression levels of α-SMA and SM22-α in HUVECs were assessed by qPCR and western blot assays. Data shown are mean ± SD and from three independent experiments. * P
    Figure Legend Snippet: Knockdown of miR-98-5p reversed si-PAPPA induced the effect on proliferation, apoptosis, and inflammatory response in HUVECs. (a and b) The interference efficiency of si-PAPPA in HUVECs was measured using qPCR and western blot assays. (c–f) HUVECs were treated with ox-LDL + si-NC, ox-LDL + si-PAPPA, ox-LDL + si-PAPPA + anti-NC, or ox-LDL + si-PAPPA + anti-miR-98-5p. (c) The cell viability of HUVECs was estimated by MTT assay. (d) Apoptosis analysis was performed in HUVECs using flow cytometry assay. (e) The western blot assay was carried out to analyze Bax and Bcl-2 expression in HUVECs. (f) The LDH detection kit was used to test LDH release in HUVECs. (g) ELISA assay was applied to measure the levels of IL-6, IL-1β, and TNF-α in the medium, with 0 μg/mL of ox-LDL group as control. (h and i) The expression levels of α-SMA and SM22-α in HUVECs were assessed by qPCR and western blot assays. Data shown are mean ± SD and from three independent experiments. * P

    Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, MTT Assay, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

    3) Product Images from "The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis"

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2018.6397

    The contents of inflammatory factors in the rats. (A) The content of TNF-α; (B) the content of IL-6. The contents of IL-6 and TNF-α in the plasma of the rats in the model group are notably higher than those of the rats in the control group (**P
    Figure Legend Snippet: The contents of inflammatory factors in the rats. (A) The content of TNF-α; (B) the content of IL-6. The contents of IL-6 and TNF-α in the plasma of the rats in the model group are notably higher than those of the rats in the control group (**P

    Techniques Used:

    4) Product Images from "Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice"

    Article Title: Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18487

    Effect of Ilomastat on the generations of cytokines in the BALF detected by ELISA The lungs removed from mice were homogenized and centrifuged. Supernatants were measured by the ELISA kits of TGF-β A. , IL-6 B. , IL-1β C. and TNF-α D. The data were obtained from lungs of four to five mice at per time point and indicate mean values ± SE. ** P
    Figure Legend Snippet: Effect of Ilomastat on the generations of cytokines in the BALF detected by ELISA The lungs removed from mice were homogenized and centrifuged. Supernatants were measured by the ELISA kits of TGF-β A. , IL-6 B. , IL-1β C. and TNF-α D. The data were obtained from lungs of four to five mice at per time point and indicate mean values ± SE. ** P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay

    5) Product Images from "ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment"

    Article Title: ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment

    Journal: Acta Biomaterialia

    doi: 10.1016/j.actbio.2022.06.024

    Expression of (A, D) TNF-α, (B, E) IL-1β and (C, F) IL-6 in (A-C) RAW264.7 and (D-F) A549 cells being stimulated by LPS for 24 h at 37 °C and treated with different materials (n=4). The dotted horizontal line represents the mean of PFTU@DEX NPs group. ANOVA followed by Tukey-test. Data are expressed as mean ± SD ⁎⁎ P
    Figure Legend Snippet: Expression of (A, D) TNF-α, (B, E) IL-1β and (C, F) IL-6 in (A-C) RAW264.7 and (D-F) A549 cells being stimulated by LPS for 24 h at 37 °C and treated with different materials (n=4). The dotted horizontal line represents the mean of PFTU@DEX NPs group. ANOVA followed by Tukey-test. Data are expressed as mean ± SD ⁎⁎ P

    Techniques Used: Expressing

    Inhalation of PFTU@DEX NPs inhibited the inflammatory factors and cell apoptosis. The secretion of IFN-γ, TNF-α and IL-6 in BALF (A) and serum (B) evaluated by LEGENDplex TM multianalyte flow assay (n = 3-4). (C) Cell apoptosis 72 h after LPS inhalation and different treatments in injured area determined by TUNEL staining (n = 5). The dotted horizontal line represents the mean of PFTU@DEX NPs group. ANOVA followed by Tukey-test. Data are expressed as means ± SD. *P
    Figure Legend Snippet: Inhalation of PFTU@DEX NPs inhibited the inflammatory factors and cell apoptosis. The secretion of IFN-γ, TNF-α and IL-6 in BALF (A) and serum (B) evaluated by LEGENDplex TM multianalyte flow assay (n = 3-4). (C) Cell apoptosis 72 h after LPS inhalation and different treatments in injured area determined by TUNEL staining (n = 5). The dotted horizontal line represents the mean of PFTU@DEX NPs group. ANOVA followed by Tukey-test. Data are expressed as means ± SD. *P

    Techniques Used: TUNEL Assay, Staining

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    Boster Bio il 6 elisa kits
    Effects of HB on LPS-induced production of <t>IL-6</t> ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by <t>ELISA</t> kits. The values are presented as means ± SD of three independent experiments. * p
    Il 6 Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 6 elisa kits/product/Boster Bio
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    il 6 elisa kits - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

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    Effects of HB on LPS-induced production of IL-6 ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by ELISA kits. The values are presented as means ± SD of three independent experiments. * p

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Heterophyllin B Ameliorates Lipopolysaccharide-Induced Inflammation and Oxidative Stress in RAW 264.7 Macrophages by Suppressing the PI3K/Akt Pathways

    doi: 10.3390/molecules23040717

    Figure Lengend Snippet: Effects of HB on LPS-induced production of IL-6 ( a ) and IL-1β ( b ) in RAW 264.7 Cells. RAW 264.7 Cells were pretreated with different concentrations of HB (25, 50, and 100 μM) for 1 h before treatment with 1 μg/mL LPS. After incubation for 18 h, the IL-6 and IL-1β production analysis was detected by ELISA kits. The values are presented as means ± SD of three independent experiments. * p

    Article Snippet: IL-1β and IL-6 ELISA kits were obtained from BOSTER (Wuhan, China).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    Knockdown of XIST weakened ox-LDL-induced effects on HUVECs. (a) The expression level of XIST in HUVECs treated with ox-LDL was determined by qPCR analysis. (b) The interference efficiency of si-XIST in HUVECs was assessed by qPCR assay. (c–f) HUVECs were divided into two groups: ox-LDL + si-NC and ox-LDL + si-XIST groups. (c) The proliferation capability of HUVECs was measured using MTT assay. (d) The flow cytometry assay was recruited to monitor the apoptosis of HUVECs. (e) The western blot analysis was applied to assess Bax and Bcl-2 expression in HUVECs. (f) A commercial available kit was used to assess LDH release in HUVECs supernatants. (g) The expression levels of IL-6, IL-1β, and TNF-α in ox-LDL-induced HUVECs were displayed, with 0 μg/mL of ox-LDL group as control. (h and i) QPCR and western blot analyses were employed to quantify mRNA and protein levels of α-SMA and SM22-α in HUVECs, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Journal: Open Medicine

    Article Title: LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

    doi: 10.1515/med-2021-0200

    Figure Lengend Snippet: Knockdown of XIST weakened ox-LDL-induced effects on HUVECs. (a) The expression level of XIST in HUVECs treated with ox-LDL was determined by qPCR analysis. (b) The interference efficiency of si-XIST in HUVECs was assessed by qPCR assay. (c–f) HUVECs were divided into two groups: ox-LDL + si-NC and ox-LDL + si-XIST groups. (c) The proliferation capability of HUVECs was measured using MTT assay. (d) The flow cytometry assay was recruited to monitor the apoptosis of HUVECs. (e) The western blot analysis was applied to assess Bax and Bcl-2 expression in HUVECs. (f) A commercial available kit was used to assess LDH release in HUVECs supernatants. (g) The expression levels of IL-6, IL-1β, and TNF-α in ox-LDL-induced HUVECs were displayed, with 0 μg/mL of ox-LDL group as control. (h and i) QPCR and western blot analyses were employed to quantify mRNA and protein levels of α-SMA and SM22-α in HUVECs, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Article Snippet: 2.6 Enzyme-linked immunosorbent assay (ELISA)The concentrations of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) in medium were evaluated by IL-6 ELISA KIT, IL-1β ELISA KIT, and TNF-α ELISA KIT (Boster, Wuhan, China) as instructed by the manufacturer, respectively.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, MTT Assay, Flow Cytometry, Western Blot

    Ox-LDL suppressed proliferation and enhanced apoptosis and inflammatory response in HUVECs. (a and b) The cell viability of HUVECs exposed to ox-LDL was assessed by MTT assay. (c) The flow cytometry assay was performed for examining the apoptosis rate of HUVECs treated with 40 μg/mL of ox-LDL for 48 h. (d) The protein expression levels of Bax and Bcl-2 were measured by western blot assay. (e) LDH release in the medium was examined by a commercial kit. (f) The levels of IL-6, IL-1β, and TNF-α were evaluated in the medium by matched kits. (g and h) The qPCR and western blot assays were used to show the expression levels of α-SMA and SM22-α in HUVECs treated with ox-LDL. Data shown are mean ± SD and from three independent experiments. * P

    Journal: Open Medicine

    Article Title: LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

    doi: 10.1515/med-2021-0200

    Figure Lengend Snippet: Ox-LDL suppressed proliferation and enhanced apoptosis and inflammatory response in HUVECs. (a and b) The cell viability of HUVECs exposed to ox-LDL was assessed by MTT assay. (c) The flow cytometry assay was performed for examining the apoptosis rate of HUVECs treated with 40 μg/mL of ox-LDL for 48 h. (d) The protein expression levels of Bax and Bcl-2 were measured by western blot assay. (e) LDH release in the medium was examined by a commercial kit. (f) The levels of IL-6, IL-1β, and TNF-α were evaluated in the medium by matched kits. (g and h) The qPCR and western blot assays were used to show the expression levels of α-SMA and SM22-α in HUVECs treated with ox-LDL. Data shown are mean ± SD and from three independent experiments. * P

    Article Snippet: 2.6 Enzyme-linked immunosorbent assay (ELISA)The concentrations of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) in medium were evaluated by IL-6 ELISA KIT, IL-1β ELISA KIT, and TNF-α ELISA KIT (Boster, Wuhan, China) as instructed by the manufacturer, respectively.

    Techniques: MTT Assay, Flow Cytometry, Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Ox-LDL repressed proliferation and induced apoptosis and inflammatory response in HUVECs by targeting the XIST/miR-98-5p axis. (a) QPCR was enforced to confirm the overexpression efficiency of miR-98-5p in HUVECs. (b–i) HUVECs were treated with ox-LDL + miR-NC, ox-LDL + miR-98-5p, ox-LDL + miR-98-5p + pcDNA, or ox-LDL + miR-98-5p + pcDNA-XIST. (b) MTT assay was used to assess cell viability of HUVECs. (c) Cells apoptosis assay was performed in transfected HUVECs by flow cytometry assay. (d and e) The protein expression levels of Bax and Bcl-2 were calculated in HUVECs by western blot assay. (f) The LDH release was displayed in the different groups by kit assay. (g) The concentrations of IL-6, IL-1β, and TNF-α in the medium were presented by ELISA assay. (h and i) The mRNA and protein expression levels of α-SMA and SM22-α in HUVECs were detected by qPCR and western blot assays, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Journal: Open Medicine

    Article Title: LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

    doi: 10.1515/med-2021-0200

    Figure Lengend Snippet: Ox-LDL repressed proliferation and induced apoptosis and inflammatory response in HUVECs by targeting the XIST/miR-98-5p axis. (a) QPCR was enforced to confirm the overexpression efficiency of miR-98-5p in HUVECs. (b–i) HUVECs were treated with ox-LDL + miR-NC, ox-LDL + miR-98-5p, ox-LDL + miR-98-5p + pcDNA, or ox-LDL + miR-98-5p + pcDNA-XIST. (b) MTT assay was used to assess cell viability of HUVECs. (c) Cells apoptosis assay was performed in transfected HUVECs by flow cytometry assay. (d and e) The protein expression levels of Bax and Bcl-2 were calculated in HUVECs by western blot assay. (f) The LDH release was displayed in the different groups by kit assay. (g) The concentrations of IL-6, IL-1β, and TNF-α in the medium were presented by ELISA assay. (h and i) The mRNA and protein expression levels of α-SMA and SM22-α in HUVECs were detected by qPCR and western blot assays, respectively. Data shown are mean ± SD and from three independent experiments. * P

    Article Snippet: 2.6 Enzyme-linked immunosorbent assay (ELISA)The concentrations of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) in medium were evaluated by IL-6 ELISA KIT, IL-1β ELISA KIT, and TNF-α ELISA KIT (Boster, Wuhan, China) as instructed by the manufacturer, respectively.

    Techniques: Real-time Polymerase Chain Reaction, Over Expression, MTT Assay, Apoptosis Assay, Transfection, Flow Cytometry, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Knockdown of miR-98-5p reversed si-PAPPA induced the effect on proliferation, apoptosis, and inflammatory response in HUVECs. (a and b) The interference efficiency of si-PAPPA in HUVECs was measured using qPCR and western blot assays. (c–f) HUVECs were treated with ox-LDL + si-NC, ox-LDL + si-PAPPA, ox-LDL + si-PAPPA + anti-NC, or ox-LDL + si-PAPPA + anti-miR-98-5p. (c) The cell viability of HUVECs was estimated by MTT assay. (d) Apoptosis analysis was performed in HUVECs using flow cytometry assay. (e) The western blot assay was carried out to analyze Bax and Bcl-2 expression in HUVECs. (f) The LDH detection kit was used to test LDH release in HUVECs. (g) ELISA assay was applied to measure the levels of IL-6, IL-1β, and TNF-α in the medium, with 0 μg/mL of ox-LDL group as control. (h and i) The expression levels of α-SMA and SM22-α in HUVECs were assessed by qPCR and western blot assays. Data shown are mean ± SD and from three independent experiments. * P

    Journal: Open Medicine

    Article Title: LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

    doi: 10.1515/med-2021-0200

    Figure Lengend Snippet: Knockdown of miR-98-5p reversed si-PAPPA induced the effect on proliferation, apoptosis, and inflammatory response in HUVECs. (a and b) The interference efficiency of si-PAPPA in HUVECs was measured using qPCR and western blot assays. (c–f) HUVECs were treated with ox-LDL + si-NC, ox-LDL + si-PAPPA, ox-LDL + si-PAPPA + anti-NC, or ox-LDL + si-PAPPA + anti-miR-98-5p. (c) The cell viability of HUVECs was estimated by MTT assay. (d) Apoptosis analysis was performed in HUVECs using flow cytometry assay. (e) The western blot assay was carried out to analyze Bax and Bcl-2 expression in HUVECs. (f) The LDH detection kit was used to test LDH release in HUVECs. (g) ELISA assay was applied to measure the levels of IL-6, IL-1β, and TNF-α in the medium, with 0 μg/mL of ox-LDL group as control. (h and i) The expression levels of α-SMA and SM22-α in HUVECs were assessed by qPCR and western blot assays. Data shown are mean ± SD and from three independent experiments. * P

    Article Snippet: 2.6 Enzyme-linked immunosorbent assay (ELISA)The concentrations of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) in medium were evaluated by IL-6 ELISA KIT, IL-1β ELISA KIT, and TNF-α ELISA KIT (Boster, Wuhan, China) as instructed by the manufacturer, respectively.

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, MTT Assay, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

    The contents of inflammatory factors in the rats. (A) The content of TNF-α; (B) the content of IL-6. The contents of IL-6 and TNF-α in the plasma of the rats in the model group are notably higher than those of the rats in the control group (**P

    Journal: Experimental and Therapeutic Medicine

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis

    doi: 10.3892/etm.2018.6397

    Figure Lengend Snippet: The contents of inflammatory factors in the rats. (A) The content of TNF-α; (B) the content of IL-6. The contents of IL-6 and TNF-α in the plasma of the rats in the model group are notably higher than those of the rats in the control group (**P

    Article Snippet: TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany).

    Techniques:

    Effect of Ilomastat on the generations of cytokines in the BALF detected by ELISA The lungs removed from mice were homogenized and centrifuged. Supernatants were measured by the ELISA kits of TGF-β A. , IL-6 B. , IL-1β C. and TNF-α D. The data were obtained from lungs of four to five mice at per time point and indicate mean values ± SE. ** P

    Journal: Oncotarget

    Article Title: Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice

    doi: 10.18632/oncotarget.18487

    Figure Lengend Snippet: Effect of Ilomastat on the generations of cytokines in the BALF detected by ELISA The lungs removed from mice were homogenized and centrifuged. Supernatants were measured by the ELISA kits of TGF-β A. , IL-6 B. , IL-1β C. and TNF-α D. The data were obtained from lungs of four to five mice at per time point and indicate mean values ± SE. ** P

    Article Snippet: Cytokine assay Transforming growth factor-β (TGF-β) and interleukin-6 (IL-6) Enzyme-Linked Immunosorbent Assay (ELISA) kits were purchased from Wuhan Boster Biological Technology Co.Ltd (Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay