e8200  (New England Biolabs)


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  • 89
    Name:
    pMAL Protein Fusion and Purification System
    Description:
    pMAL Protein Fusion and Purification System
    Catalog Number:
    E8200S
    Price:
    645
    Category:
    E coli Protein Expression Kits
    Score:
    85
    Buy from Supplier


    Structured Review

    New England Biolabs e8200
    pMAL Protein Fusion and Purification System
    pMAL Protein Fusion and Purification System
    https://www.bioz.com/result/e8200/product/New England Biolabs
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e8200 - by Bioz Stars, 2019-11
    89/100 stars

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    Related Articles

    Chick Chorioallantoic Membrane Assay:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively. .. IPD3 was phosphorylated in vitro by CCaMK as described .

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. IPD3 was phosphorylated in vitro by DMI3 as described (Jin et al., ).

    In Vitro:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Paragraph title: In vitro phosphorylation ... Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Paragraph title: In vitro phosphorylation and mass spectrometric analysis ... Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. Each reaction was carried out with 1 mg MBP-DMI3 protein, 0.2 mM CaCl2 , 0.5 mM bovine CaM, 2 mg 6XHIS-IPD3 as substrate.

    Purification:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Denatured proteins were refolded by stepwise dialysis . .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively. .. IPD3 was phosphorylated in vitro by CCaMK as described .

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Denatured proteins were refolded by stepwise dialysis (Yano et al., ). .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. IPD3 was phosphorylated in vitro by DMI3 as described (Jin et al., ).

    Expressing:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under the denaturing conditions by using 8 M urea. .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under denaturing conditions using 8 M urea. .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Affinity Purification:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under the denaturing conditions by using 8 M urea. .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under denaturing conditions using 8 M urea. .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Liquid Chromatography:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. Each reaction was carried out with 1 mg MBP-DMI3 protein, 0.2 mM CaCl2 , 0.5 mM bovine CaM, 2 mg 6XHIS-IPD3 as substrate.

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  • News
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  • Bioz Stars
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  • 89
    New England Biolabs e8200
    E8200, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e8200/product/New England Biolabs
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e8200 - by Bioz Stars, 2019-11
    89/100 stars
      Buy from Supplier

    95
    New England Biolabs mbp
    Simple Blue TM staining of purified recombinant human <t>MBP-tagged</t> Nmnat 1, mutant, and wild-type <t>Nampts</t> separated by SDS-PAGE. Recombinant human proteins were expressed in E. coli and purified as described under “Experimental Procedures.”
    Mbp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbp/product/New England Biolabs
    Average 95 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    mbp - by Bioz Stars, 2019-11
    95/100 stars
      Buy from Supplier

    Image Search Results


    Simple Blue TM staining of purified recombinant human MBP-tagged Nmnat 1, mutant, and wild-type Nampts separated by SDS-PAGE. Recombinant human proteins were expressed in E. coli and purified as described under “Experimental Procedures.”

    Journal:

    Article Title: Regulation of Inflammatory Cytokine Expression in Pulmonary Epithelial Cells by Pre-B-cell Colony-enhancing Factor via a Nonenzymatic and AP-1-dependent Mechanism

    doi: 10.1074/jbc.M109.002519

    Figure Lengend Snippet: Simple Blue TM staining of purified recombinant human MBP-tagged Nmnat 1, mutant, and wild-type Nampts separated by SDS-PAGE. Recombinant human proteins were expressed in E. coli and purified as described under “Experimental Procedures.”

    Article Snippet: MBP-Nmnat 1 expressed in Escherichia coli strain K12 TB1 or Nampt and mutant Nampts in E. coli BL21 were purified by affinity purification specific for MBP (cat. no. E8200, New England Biolabs) or glutathione S -transferase resin (cat. no. 27-0843-01, Amersham Biosciences), respectively.

    Techniques: Staining, Purification, Recombinant, Mutagenesis, SDS Page