ph d 12 peptide library  (New England Biolabs)


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    Name:
    Ph D 12 Phage Display Peptide Library Kit
    Description:
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    Catalog Number:
    e8110s
    Price:
    564
    Size:
    10 exps
    Category:
    Phage Display Systems
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    New England Biolabs ph d 12 peptide library
    Ph D 12 Phage Display Peptide Library Kit
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    https://www.bioz.com/result/ph d 12 peptide library/product/New England Biolabs
    Average 93 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    ph d 12 peptide library - by Bioz Stars, 2021-01
    93/100 stars

    Images

    1) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    2) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    3) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    4) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    5) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    Related Articles

    Incubation:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. After washing 5 times with TBS containing 0.05% (v/v) Tween 20 (TBST), 100 μL Ph.D.-12 peptide library (New England BioLabs, Inc., USA) aliquot containing 1011 phages was added to each well and the plate was incubated for 1 hour at 37 °C. ..

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. Effect of pH changes on phage binding In order to study the effect of pH on phage binding to polystyrene, 1010 pfu of PB-TUP, Ph.D. -12 peptide library, M13KE, and D12 clone were incubated in solutions with varying pH values and were added to the blocked wells of the polystyrene microtiter plate. .. Phage binding was tested by ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 1010 pfu of possible polystyrene binding clone PB-TUP, Ph.D.-12 peptide library, M13KE (LacZa(-) wild-type M13 phage, New England BioLabs, Inc., USA), and a non-relevant phage D12 clone was added into the microtiter plates as a control group and the plates were incubated overnight at 4 °C. .. Then the plates were washed 5 times with the same buffer which was used for incubation, and the relative number of bound phage was determined by phage ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 1010 pfu of PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added to the microtiter plates and incubated overnight at 4 °C. .. Next day, the plates were washed 3 times with TBST and 3 times with TBS and the relative number of bound phage was determined by phage ELISA.

    Amplification:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 106 PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added separately into the logarithmic growth phase culture for infection and amplification. .. Concentration dependent binding to polystyreneDifferent concentrations of PB-TUP, Ph.D.-12 library, M13KE, and D12 clone were individually added into the blocked microtiter wells.

    Binding Assay:

    Article Title: Identification of a New Tuberculosis Antigen Recognized by ?? T Cell Receptor
    Article Snippet: .. The phD 12 phage display peptide library kit (New England Biolabs) was used to screen specific peptides binding to γδ TCR. .. J.RT3-T3.5 cells and THP-1 cells, a human myelomonocytic cell line, were obtained from the American Type Culture Collection (ATCC). γδ T cells, immobilized by anti-pan-γδ TCR monoclonal antibody (Immunotech), 1-deoxy- d -xylulose 5-phosphate synthase 2 (DXS2) protein, and extracellular peptide (EP) were obtained from fresh peripheral blood mononuclear cells (PBMC).

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. Effect of pH changes on phage binding In order to study the effect of pH on phage binding to polystyrene, 1010 pfu of PB-TUP, Ph.D. -12 peptide library, M13KE, and D12 clone were incubated in solutions with varying pH values and were added to the blocked wells of the polystyrene microtiter plate. .. Phage binding was tested by ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 1010 pfu of possible polystyrene binding clone PB-TUP, Ph.D.-12 peptide library, M13KE (LacZa(-) wild-type M13 phage, New England BioLabs, Inc., USA), and a non-relevant phage D12 clone was added into the microtiter plates as a control group and the plates were incubated overnight at 4 °C. .. Then the plates were washed 5 times with the same buffer which was used for incubation, and the relative number of bound phage was determined by phage ELISA.

    Infection:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 106 PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added separately into the logarithmic growth phase culture for infection and amplification. .. Concentration dependent binding to polystyreneDifferent concentrations of PB-TUP, Ph.D.-12 library, M13KE, and D12 clone were individually added into the blocked microtiter wells.

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  • 93
    New England Biolabs ph d 12 peptide library
    Polystyrene binding of PB-TUP, <t>Ph.D.-12</t> peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Ph D 12 Peptide Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ph d 12 peptide library/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ph d 12 peptide library - by Bioz Stars, 2021-01
    93/100 stars
      Buy from Supplier

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    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Journal: Scientific Reports

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    doi: 10.1038/s41598-017-02891-x

    Figure Lengend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Article Snippet: 1010 pfu of PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added to the microtiter plates and incubated overnight at 4 °C.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay