phage peptide library  (New England Biolabs)


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  • 98
    Name:
    Ph D 12 Phage Display Peptide Library Kit
    Description:
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    Catalog Number:
    e8110s
    Price:
    564
    Size:
    10 exps
    Category:
    Phage Display Systems
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    Structured Review

    New England Biolabs phage peptide library
    Ph D 12 Phage Display Peptide Library Kit
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    https://www.bioz.com/result/phage peptide library/product/New England Biolabs
    Average 98 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    phage peptide library - by Bioz Stars, 2020-08
    98/100 stars

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    Related Articles

    Selection:

    Article Title: Novel Method for Selection of Antimicrobial Peptides from a Phage Display Library by Use of Bacterial Magnetic Particles ▿
    Article Snippet: .. The Ph.D.-12 phage display peptide library kit with a complexity of 2.7 × 109 (New England BioLabs, Beverly, MA) was used for six rounds of affinity selection. .. Selection against the BacMP membrane was performed by incubating approximately 4 × 1010 PFU of the library phage (complexity, 2.7 × 109 ) with BacMPs (300 μg) in 50 mM Tris buffer containing 150 mM sodium chloride and 0.1% Tween 20 (pH 7.5) for 1 h at room temperature with agitation.

    Magnetic Beads:

    Article Title: Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum
    Article Snippet: .. TBS, bovine serum albumin (BSA), Tween 20, disodium hydrogen phosphate (Na2 HPO4 ), sodium hydrogen phosphate (NaHPO4 ) salt, sodium chloride (NaCl), polyethylene glycol 8000 (PEG8000), p -nitrophenyl phosphate tablets, IPTG, X-gal, imidazole, fluorescein isothiocyanate (FITC), disodium carbonate (Na2 CO3 ), sodium carbonate monobasic (NaHCO3 ), amino ethyl methacrylate hydrochloride (AEMA), MES, N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide (EDC), polyethylene glycol dimethacrylate (PEGMA), methylene bisacrylamide (MBA), potassium persulfate (KPS), tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St Louis, MO, USA); the Ph.D.-12TM phage display peptide library kit was purchased from New England Biolabs (Beverly, MA, USA); hexahistidine-tagged recombinant human TNFα (6His-rhTNFα) was supplied by Prospec (East Brunswick, NJ, USA); nickel-coated magnetic beads (NiMB: ø 10 µm, ρ 1.1 g dm−3 ) were purchased from Kisker (Steinfurt, Germany); nickel-coated 96 multiwell plates were purchased from Thermo Scientific (Waltham, MA, USA); poly-dimethylsiloxane (PDMS) from Emanuele Mascherpa S.p.A (Milan, Italy), Dynabeads MyOne carboxylic (MP) from Invitrogen Dynal AS (Oslo, Norway). .. Reagents for peptide synthesis (Fmoc-protected amino acids, resins, activation and deprotection reagents) were from Novabiochem (Laufelfingen, Switzerland) and InBios (Naples, Italy).

    Purification:

    Article Title: Identification of a New Broadly Cross-reactive Epitope within Domain III of the Duck Tembusu Virus E Protein
    Article Snippet: .. Epitope mapping The epitope was mapped with purified 3B6 mAb using the Ph.D-12TM Phage Display Peptide Library Kit (New England BioLabs), as previously described . ..

    Incubation:

    Article Title: Competitive Mirror Image Phage Display Derived Peptide Modulates Amyloid Beta Aggregation and Toxicity
    Article Snippet: .. In the first panning round 90 μl of 1x TBS were mixed with 10 μl of the Ph.D.-12 Phage Display Peptide Library (New England Biolabs GmbH, Frankfurt/M, Germany) and added to the well for five minutes of incubation at RT. .. The solution was removed and 100 μl of 10 μM biotin in 1x TBS / 0.1% (v/v) Tween-20 (TBS-T) were added for five minutes incubation at RT to reduce streptavidin-binding phages.

    Expressing:

    Article Title: Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase
    Article Snippet: .. The recombinant bacteria expressing pAPN-C were stored at the Department of Veterinary Clinical Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University. The Ph.D.-12 Phage Display Peptide Library Kit was purchased from New England BioLabs (Beverly, MA). .. Purification of the recombinant pAPN-C protein (rpAPN-C) was performed according to the method described by Zhu and colleagues. Briefly, the recombinant pAPN-C-expressing bacteria were induced with 0.8 mM IPTG at 37°C for 4 h and then the rpAPN-C inclusion bodies were extracted.

    Recombinant:

    Article Title: Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum
    Article Snippet: .. TBS, bovine serum albumin (BSA), Tween 20, disodium hydrogen phosphate (Na2 HPO4 ), sodium hydrogen phosphate (NaHPO4 ) salt, sodium chloride (NaCl), polyethylene glycol 8000 (PEG8000), p -nitrophenyl phosphate tablets, IPTG, X-gal, imidazole, fluorescein isothiocyanate (FITC), disodium carbonate (Na2 CO3 ), sodium carbonate monobasic (NaHCO3 ), amino ethyl methacrylate hydrochloride (AEMA), MES, N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide (EDC), polyethylene glycol dimethacrylate (PEGMA), methylene bisacrylamide (MBA), potassium persulfate (KPS), tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St Louis, MO, USA); the Ph.D.-12TM phage display peptide library kit was purchased from New England Biolabs (Beverly, MA, USA); hexahistidine-tagged recombinant human TNFα (6His-rhTNFα) was supplied by Prospec (East Brunswick, NJ, USA); nickel-coated magnetic beads (NiMB: ø 10 µm, ρ 1.1 g dm−3 ) were purchased from Kisker (Steinfurt, Germany); nickel-coated 96 multiwell plates were purchased from Thermo Scientific (Waltham, MA, USA); poly-dimethylsiloxane (PDMS) from Emanuele Mascherpa S.p.A (Milan, Italy), Dynabeads MyOne carboxylic (MP) from Invitrogen Dynal AS (Oslo, Norway). .. Reagents for peptide synthesis (Fmoc-protected amino acids, resins, activation and deprotection reagents) were from Novabiochem (Laufelfingen, Switzerland) and InBios (Naples, Italy).

    Article Title: Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase
    Article Snippet: .. The recombinant bacteria expressing pAPN-C were stored at the Department of Veterinary Clinical Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University. The Ph.D.-12 Phage Display Peptide Library Kit was purchased from New England BioLabs (Beverly, MA). .. Purification of the recombinant pAPN-C protein (rpAPN-C) was performed according to the method described by Zhu and colleagues. Briefly, the recombinant pAPN-C-expressing bacteria were induced with 0.8 mM IPTG at 37°C for 4 h and then the rpAPN-C inclusion bodies were extracted.

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  • 98
    New England Biolabs phage peptide library
    Phage Peptide Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phage peptide library/product/New England Biolabs
    Average 98 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    phage peptide library - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

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