ph d 12 peptide library  (New England Biolabs)


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    Name:
    Ph D 12 Phage Display Peptide Library Kit
    Description:
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    Catalog Number:
    E8110S
    Price:
    564
    Category:
    Phage Display Systems
    Size:
    10 exps
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    New England Biolabs ph d 12 peptide library
    Ph D 12 Phage Display Peptide Library Kit
    Ph D 12 Phage Display Peptide Library Kit 10 panning exps
    https://www.bioz.com/result/ph d 12 peptide library/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ph d 12 peptide library - by Bioz Stars, 2021-05
    98/100 stars

    Images

    1) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    2) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    3) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    4) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    5) Product Images from "Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization"

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02891-x

    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Figure Legend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay

    Related Articles

    Incubation:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: Evaluation of phage binding to blocking buffer 96-well polystyrene microtiter plates were blocked at 37 °C for 2 hours with different concentration (0.00, 0.01, 0.10, 0.20, 0.40, 0.80, 1.60, 3.20%) (w/v) NFM-TBS. .. 1010 pfu of PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added to the microtiter plates and incubated overnight at 4 °C. .. Next day, the plates were washed 3 times with TBST and 3 times with TBS and the relative number of bound phage was determined by phage ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: The next day, each well was blocked with 250 μL 3% (w/v) non-fat milk (NFM) (Sangon Biotech Co., Ltd., Shanghai) for 2 hours at 37 °C. .. After washing 5 times with TBS containing 0.05% (v/v) Tween 20 (TBST), 100 μL Ph.D.-12 peptide library (New England BioLabs, Inc., USA) aliquot containing 1011 phages was added to each well and the plate was incubated for 1 hour at 37 °C. ..

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: ELISA was used to determine the phage binding and the titer of the recovered phage was determined by LB/IPTG/X-gal plate. .. Effect of pH changes on phage binding In order to study the effect of pH on phage binding to polystyrene, 1010 pfu of PB-TUP, Ph.D. -12 peptide library, M13KE, and D12 clone were incubated in solutions with varying pH values and were added to the blocked wells of the polystyrene microtiter plate. .. Phage binding was tested by ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: Polystyrene binding study 96-well polystyrene microtiter plates were blocked at 37 °C for 2 hours with different blocking buffers including PBS, 0.05% PBST, TBS, 0.05% TBST, 3% BSA-TBS and 3% NFM-TBS separately. .. 1010 pfu of possible polystyrene binding clone PB-TUP, Ph.D.-12 peptide library, M13KE (LacZa(-) wild-type M13 phage, New England BioLabs, Inc., USA), and a non-relevant phage D12 clone was added into the microtiter plates as a control group and the plates were incubated overnight at 4 °C. .. Then the plates were washed 5 times with the same buffer which was used for incubation, and the relative number of bound phage was determined by phage ELISA.

    Infection:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 106 PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added separately into the logarithmic growth phase culture for infection and amplification. .. Concentration dependent binding to polystyreneDifferent concentrations of PB-TUP, Ph.D.-12 library, M13KE, and D12 clone were individually added into the blocked microtiter wells.

    Amplification:

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: .. 106 PB-TUP, Ph.D.-12 peptide library, M13KE, and D12 clone were added separately into the logarithmic growth phase culture for infection and amplification. .. Concentration dependent binding to polystyreneDifferent concentrations of PB-TUP, Ph.D.-12 library, M13KE, and D12 clone were individually added into the blocked microtiter wells.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) The relative binding affinities of individual phage clones to GST-CAS SH3 were assessed by ELISA according to the manufacturer’s protocol (Ph.D.™ −12 Phage Display Peptide Library Kit, New England Biolabs). ..

    Binding Assay:

    Article Title: Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) The relative binding affinities of individual phage clones to GST-CAS SH3 were assessed by ELISA according to the manufacturer’s protocol (Ph.D.™ −12 Phage Display Peptide Library Kit, New England Biolabs). ..

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: ELISA was used to determine the phage binding and the titer of the recovered phage was determined by LB/IPTG/X-gal plate. .. Effect of pH changes on phage binding In order to study the effect of pH on phage binding to polystyrene, 1010 pfu of PB-TUP, Ph.D. -12 peptide library, M13KE, and D12 clone were incubated in solutions with varying pH values and were added to the blocked wells of the polystyrene microtiter plate. .. Phage binding was tested by ELISA.

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
    Article Snippet: Polystyrene binding study 96-well polystyrene microtiter plates were blocked at 37 °C for 2 hours with different blocking buffers including PBS, 0.05% PBST, TBS, 0.05% TBST, 3% BSA-TBS and 3% NFM-TBS separately. .. 1010 pfu of possible polystyrene binding clone PB-TUP, Ph.D.-12 peptide library, M13KE (LacZa(-) wild-type M13 phage, New England BioLabs, Inc., USA), and a non-relevant phage D12 clone was added into the microtiter plates as a control group and the plates were incubated overnight at 4 °C. .. Then the plates were washed 5 times with the same buffer which was used for incubation, and the relative number of bound phage was determined by phage ELISA.

    Clone Assay:

    Article Title: Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) The relative binding affinities of individual phage clones to GST-CAS SH3 were assessed by ELISA according to the manufacturer’s protocol (Ph.D.™ −12 Phage Display Peptide Library Kit, New England Biolabs). ..

    other:

    Article Title: Screening of a Specific Peptide Binding to VPAC1 Receptor from a Phage Display Peptide Library
    Article Snippet: Reagents The Ph.D.-12™ phage display peptide library kit containing E.coli host strain ER2738 was purchased from New England BioLabs (Ipswich, MA, USA).

    Purification:

    Article Title: Epitope Identification and Application for Diagnosis of Duck Tembusu Virus Infections in Ducks
    Article Snippet: The purified immunoglobulin (Ig) G antibody concentrations were determined by measuring the absorbance at 278 nm. .. Epitope Mapping The epitopes were mapped with purified 1A5 and 1F3 using the Ph.D-12™ Phage Display Peptide Library Kit (New England BioLabs Inc., Ipswich, MA, USA) as previously described [ , ]. ..

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  • 98
    New England Biolabs ph d 12 peptide library
    Polystyrene binding of PB-TUP, <t>Ph.D.-12</t> peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.
    Ph D 12 Peptide Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ph d 12 peptide library/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ph d 12 peptide library - by Bioz Stars, 2021-05
    98/100 stars
      Buy from Supplier

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    Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Journal: Scientific Reports

    Article Title: Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization

    doi: 10.1038/s41598-017-02891-x

    Figure Lengend Snippet: Polystyrene binding of PB-TUP, Ph.D.-12 peptide library, M13KE, and a non-relevant phage clone (D12) determined by ELISA. Four phage clones were added to the microtiter plates and the wells were treated with six different blocking buffers (first line) and six different washing buffers (second line), separately. ELISA values were used to evaluate the binding ability of these four phage clones to polystyrene. NFM had the best blocking effect. PBST and TBST were more effective washing buffers than the others. PB-TUP phage clone always had much higher absorbance comparing with the other phage clones. The experiments were performed in triplicates and repeated twice.

    Article Snippet: 1010 pfu of possible polystyrene binding clone PB-TUP, Ph.D.-12 peptide library, M13KE (LacZa(-) wild-type M13 phage, New England BioLabs, Inc., USA), and a non-relevant phage D12 clone was added into the microtiter plates as a control group and the plates were incubated overnight at 4 °C.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Blocking Assay