monoclonal anti mbp hrp conjugate  (New England Biolabs)


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    Structured Review

    New England Biolabs monoclonal anti mbp hrp conjugate
    Monoclonal Anti Mbp Hrp Conjugate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti mbp hrp conjugate/product/New England Biolabs
    Average 85 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti mbp hrp conjugate - by Bioz Stars, 2020-07
    85/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Protective Antibodies Induced by a Novel Epitope of Human TNF-? Could Suppress the Development of Collagen-Induced Arthritis
    Article Snippet: .. The Ph.D.™ Peptide Display Cloning System was also purchased from New England Biolabs. .. The E. coli strain BL21 was purchased from Novagen.

    Article Title: Subunit disassembly and inhibition of TNFα by a semi-synthetic bicyclic peptide
    Article Snippet: .. The pMAL-pIII vector ( ) system was chosen because it was designed for cloning selected peptide sequences from the New England Biolabs Ph.D. Phage Display libraries as N-terminal fusions to MBP (maltose binding protein, encoded by the malE gene). ..

    Article Title: Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide
    Article Snippet: .. To date, some random peptide libraries have been reported, – and some libraries or phage peptide display cloning systems have also been commercialized (New England Biolabs). ..

    Article Title: Filamentous Virus-based Assembly: Their Oriented Structures and Thermal Diffusivity
    Article Snippet: .. The phages were expressed using the Ph.D. Peptide Display Cloning System (New England Biolabs, Inc.). ..

    Article Title: Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer
    Article Snippet: .. To verify the ability of the phage to penetrate the skin, the Ph.D. Peptide Display Cloning System (M13KE vector, New England Biolabs) was used to create phage which displayed specific peptide sequences of interest. .. The peptide sequence for the SPACE peptide was ACTGSTQHQCG and the peptide sequence for the control peptide (CP) was ACTHGQTQSCG with the formation of the disulfide bond between the cysteines to produce a cyclic peptide.

    Article Title: Filamentous Virus-based Assembly: Their Oriented Structures and Thermal Diffusivity
    Article Snippet: .. Preparation of phages The phages were expressed using the Ph.D. Peptide Display Cloning System (New England Biolabs, Inc.). ..

    Amplification:

    Article Title: Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants
    Article Snippet: .. Individual SECIS variants were amplified by PCR from ∼100 ng of single stranded phage DNA, or from M13KE ligation reactions if phage was not isolated, using the duplex extension primer 5′-CATGCCCGGGTACCTTTCTATTCTC-3′ (NEB product E8101S) and the Bam HI primer 5′-GACGTTAGTAAATGAATTTTCTGGATCCGATTTTGC-3′. .. The amplified inserts were digested with Acc 65I and Bam HI, gel-purified, and ligated into Acc 65I/ Bam HI-digested pUC18.

    Ligation:

    Article Title: Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants
    Article Snippet: .. Individual SECIS variants were amplified by PCR from ∼100 ng of single stranded phage DNA, or from M13KE ligation reactions if phage was not isolated, using the duplex extension primer 5′-CATGCCCGGGTACCTTTCTATTCTC-3′ (NEB product E8101S) and the Bam HI primer 5′-GACGTTAGTAAATGAATTTTCTGGATCCGATTTTGC-3′. .. The amplified inserts were digested with Acc 65I and Bam HI, gel-purified, and ligated into Acc 65I/ Bam HI-digested pUC18.

    Isolation:

    Article Title: Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants
    Article Snippet: .. Individual SECIS variants were amplified by PCR from ∼100 ng of single stranded phage DNA, or from M13KE ligation reactions if phage was not isolated, using the duplex extension primer 5′-CATGCCCGGGTACCTTTCTATTCTC-3′ (NEB product E8101S) and the Bam HI primer 5′-GACGTTAGTAAATGAATTTTCTGGATCCGATTTTGC-3′. .. The amplified inserts were digested with Acc 65I and Bam HI, gel-purified, and ligated into Acc 65I/ Bam HI-digested pUC18.

    Sequencing:

    Article Title: A Phage Display Selected 7-mer Peptide Inhibitor of the Tannerella forsythia Metalloprotease-Like Enzyme Karilysin can be Truncated to Ser-Trp-Phe-Pro
    Article Snippet: .. Peptide phage libraries (7-mer and 7-mer cysteine-constrained), pMAL-pIII vector, M13KE insert extension primer (NEB #E8101), −96 gIII sequencing primer (NEB #S1259), monoclonal anti-MBP HRP-conjugate, EagI and Acc65I were from New England Biolabs. .. Maxisorp microtiter plates and black fluorescence non-surface treated plates were both from NUNC.

    Polymerase Chain Reaction:

    Article Title: Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants
    Article Snippet: .. Individual SECIS variants were amplified by PCR from ∼100 ng of single stranded phage DNA, or from M13KE ligation reactions if phage was not isolated, using the duplex extension primer 5′-CATGCCCGGGTACCTTTCTATTCTC-3′ (NEB product E8101S) and the Bam HI primer 5′-GACGTTAGTAAATGAATTTTCTGGATCCGATTTTGC-3′. .. The amplified inserts were digested with Acc 65I and Bam HI, gel-purified, and ligated into Acc 65I/ Bam HI-digested pUC18.

    Binding Assay:

    Article Title: Subunit disassembly and inhibition of TNFα by a semi-synthetic bicyclic peptide
    Article Snippet: .. The pMAL-pIII vector ( ) system was chosen because it was designed for cloning selected peptide sequences from the New England Biolabs Ph.D. Phage Display libraries as N-terminal fusions to MBP (maltose binding protein, encoded by the malE gene). ..

    Plasmid Preparation:

    Article Title: Subunit disassembly and inhibition of TNFα by a semi-synthetic bicyclic peptide
    Article Snippet: .. The pMAL-pIII vector ( ) system was chosen because it was designed for cloning selected peptide sequences from the New England Biolabs Ph.D. Phage Display libraries as N-terminal fusions to MBP (maltose binding protein, encoded by the malE gene). ..

    Article Title: A Phage Display Selected 7-mer Peptide Inhibitor of the Tannerella forsythia Metalloprotease-Like Enzyme Karilysin can be Truncated to Ser-Trp-Phe-Pro
    Article Snippet: .. Peptide phage libraries (7-mer and 7-mer cysteine-constrained), pMAL-pIII vector, M13KE insert extension primer (NEB #E8101), −96 gIII sequencing primer (NEB #S1259), monoclonal anti-MBP HRP-conjugate, EagI and Acc65I were from New England Biolabs. .. Maxisorp microtiter plates and black fluorescence non-surface treated plates were both from NUNC.

    Article Title: Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer
    Article Snippet: .. To verify the ability of the phage to penetrate the skin, the Ph.D. Peptide Display Cloning System (M13KE vector, New England Biolabs) was used to create phage which displayed specific peptide sequences of interest. .. The peptide sequence for the SPACE peptide was ACTGSTQHQCG and the peptide sequence for the control peptide (CP) was ACTHGQTQSCG with the formation of the disulfide bond between the cysteines to produce a cyclic peptide.

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  • 93
    New England Biolabs ph d peptide display cloning system
    Ph D Peptide Display Cloning System, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ph d peptide display cloning system/product/New England Biolabs
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    ph d peptide display cloning system - by Bioz Stars, 2020-07
    93/100 stars
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