ph d 7 phage display peptide library kit  (New England Biolabs)


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    Name:
    Ph D 7 Phage Display Peptide Library Kit
    Description:
    Ph D 7 Phage Display Peptide Library Kit 10 panning exps
    Catalog Number:
    e8100s
    Price:
    554
    Size:
    10 exps
    Category:
    Phage Display Systems
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    Structured Review

    New England Biolabs ph d 7 phage display peptide library kit
    Ph D 7 Phage Display Peptide Library Kit
    Ph D 7 Phage Display Peptide Library Kit 10 panning exps
    https://www.bioz.com/result/ph d 7 phage display peptide library kit/product/New England Biolabs
    Average 95 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    ph d 7 phage display peptide library kit - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries"

    Article Title: Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2019.02796

    Distribution of the amino acid residues in the mimotope library. (A) Log odds (LO) relative to background frequencies. (B) Sequence logo of the LO by position relative to the overall background frequencies. (C) Sequence logo of the frequencies by position. (D) Sequence logo of the LO by position in an amplified Ph.D.-7 phage library without ligand selection relative to the overall background frequencies (based on W. L. Matochko, R. Derda. “Error analysis of deep sequencing of phage libraries: peptides censored in sequencing”. Comput Math Methods Med , 2013, 491612. 2013., http://www.chem.ualberta.ca/~derda/parasitepaper/rawfiles/PhD7-GAC-30FuR.txt ). (E) Sequence logo of the LO by position relative to the frequencies by position in the amplified, unselected library shown in (D) . The skewing of the distribution in the free N-terminus appears to be property of the library, whereas the selection by the IgM repertoire leads to slight skewing in the middle of the sequence toward negatively charged residues, glycine and tryptophan.
    Figure Legend Snippet: Distribution of the amino acid residues in the mimotope library. (A) Log odds (LO) relative to background frequencies. (B) Sequence logo of the LO by position relative to the overall background frequencies. (C) Sequence logo of the frequencies by position. (D) Sequence logo of the LO by position in an amplified Ph.D.-7 phage library without ligand selection relative to the overall background frequencies (based on W. L. Matochko, R. Derda. “Error analysis of deep sequencing of phage libraries: peptides censored in sequencing”. Comput Math Methods Med , 2013, 491612. 2013., http://www.chem.ualberta.ca/~derda/parasitepaper/rawfiles/PhD7-GAC-30FuR.txt ). (E) Sequence logo of the LO by position relative to the frequencies by position in the amplified, unselected library shown in (D) . The skewing of the distribution in the free N-terminus appears to be property of the library, whereas the selection by the IgM repertoire leads to slight skewing in the middle of the sequence toward negatively charged residues, glycine and tryptophan.

    Techniques Used: Sequencing, Amplification, Selection

    2) Product Images from "A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids"

    Article Title: A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids

    Journal: Oncotarget

    doi: 10.18632/oncotarget.24960

    Strategies for the establishment of primary and secondary CSC HP libraries (A) Flow chart of the primary screening from the original M13 PhD7 library to prepare the EMT6 CSC HP-Library was shown in the upper part. The secondary screening tactics to produce the CT26 CSC HP-Library, the Hepa1-6 CSC HP-Library, the CSC/ESC HP-Library, and the CSC HP-Library was illustrated in the lower part. (B) The same strategy of EMT6 CSC HP-library preparation was used to establish the PANC-1 CSC HP-library.
    Figure Legend Snippet: Strategies for the establishment of primary and secondary CSC HP libraries (A) Flow chart of the primary screening from the original M13 PhD7 library to prepare the EMT6 CSC HP-Library was shown in the upper part. The secondary screening tactics to produce the CT26 CSC HP-Library, the Hepa1-6 CSC HP-Library, the CSC/ESC HP-Library, and the CSC HP-Library was illustrated in the lower part. (B) The same strategy of EMT6 CSC HP-library preparation was used to establish the PANC-1 CSC HP-library.

    Techniques Used: Flow Cytometry

    3) Product Images from "Amsacta moorei Entomopoxvirus Inhibitor of Apoptosis Suppresses Cell Death by Binding Grim and Hid"

    Article Title: Amsacta moorei Entomopoxvirus Inhibitor of Apoptosis Suppresses Cell Death by Binding Grim and Hid

    Journal: Journal of Virology

    doi: 10.1128/JVI.79.6.3684-3691.2005

    Phage display sequences identified after three rounds of phage selection and amplification. (A) Twenty representative isolates from the Ph.D.-7 library (New England BioLabs) were sequenced after each round. Recurrences of specific amino acids after the third round are indicated in parentheses. X, no significant recurrence. (B) The binding motif sequences of mammalian (human) and insect ( Drosophila ) IBM-containing proteins. The first seven amino acids of the mature forms of each protein are shown. Superscripted numbers indicate the amino acid positions of the alanine in the precursor proteins.
    Figure Legend Snippet: Phage display sequences identified after three rounds of phage selection and amplification. (A) Twenty representative isolates from the Ph.D.-7 library (New England BioLabs) were sequenced after each round. Recurrences of specific amino acids after the third round are indicated in parentheses. X, no significant recurrence. (B) The binding motif sequences of mammalian (human) and insect ( Drosophila ) IBM-containing proteins. The first seven amino acids of the mature forms of each protein are shown. Superscripted numbers indicate the amino acid positions of the alanine in the precursor proteins.

    Techniques Used: Selection, Amplification, Binding Assay

    4) Product Images from "Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants"

    Article Title: Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0030839

    Identification of LPS specific peptide mimotopes. LPS antibody was immobilized at a concentration of 1 µg per well of a 96-well plate. Phages (2×10 11 ) expressing 7-mer peptides were initially added to the well of a 96-well plate containing immobilized LPS antibody after which non-specific phages were removed. Twelve random phage clones were selected from three rounds of panning and specificity to LPS antibody (black bars) was confirmed by ELISA using HSP70 antibody (white bars) as a negative control. All 12 (RS01-RS12) clones selected displayed specific reactivity to LPS antibody, as determined using the HRP labeled anti-M13 phage antibody which was detected by the HRP substrate SIGMAFAST™ OPD and absorbance measured at 490 nm. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.
    Figure Legend Snippet: Identification of LPS specific peptide mimotopes. LPS antibody was immobilized at a concentration of 1 µg per well of a 96-well plate. Phages (2×10 11 ) expressing 7-mer peptides were initially added to the well of a 96-well plate containing immobilized LPS antibody after which non-specific phages were removed. Twelve random phage clones were selected from three rounds of panning and specificity to LPS antibody (black bars) was confirmed by ELISA using HSP70 antibody (white bars) as a negative control. All 12 (RS01-RS12) clones selected displayed specific reactivity to LPS antibody, as determined using the HRP labeled anti-M13 phage antibody which was detected by the HRP substrate SIGMAFAST™ OPD and absorbance measured at 490 nm. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.

    Techniques Used: Concentration Assay, Expressing, Clone Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Labeling, Standard Deviation

    5) Product Images from "Peptide affinity reagents for AAV capsid recognition and purification"

    Article Title: Peptide affinity reagents for AAV capsid recognition and purification

    Journal: Gene therapy

    doi: 10.1038/gt.2011.46

    ( a ) Phage panning procedure. The Ph.D.-7 phage display library was co-incubated with AAV capsids to allow binding (B), followed by washing (W) of unbound phage and elution (E) of bound phage. Bound phage were then amplified and subjected to three rounds
    Figure Legend Snippet: ( a ) Phage panning procedure. The Ph.D.-7 phage display library was co-incubated with AAV capsids to allow binding (B), followed by washing (W) of unbound phage and elution (E) of bound phage. Bound phage were then amplified and subjected to three rounds

    Techniques Used: Incubation, Binding Assay, Amplification

    6) Product Images from "Specific Targeting of Hepatitis C Virus Core Protein by an Intracellular Single-Chain Antibody of Human Origin"

    Article Title: Specific Targeting of Hepatitis C Virus Core Protein by an Intracellular Single-Chain Antibody of Human Origin

    Journal:

    doi: 10.1002/hep.22366

    Identification of scFv42C binding motif in HCV core protein by multiple sequence alignment. Core protein sequence (residues 79–88) is shown on the top, followed by deduced amino acid sequences of eight phage clones (mimotopes) from the Ph.D.-7
    Figure Legend Snippet: Identification of scFv42C binding motif in HCV core protein by multiple sequence alignment. Core protein sequence (residues 79–88) is shown on the top, followed by deduced amino acid sequences of eight phage clones (mimotopes) from the Ph.D.-7

    Techniques Used: Binding Assay, Sequencing

    Related Articles

    Concentration Assay:

    Article Title: Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries
    Article Snippet: .. A 7-mer random peptide library (E8100S, Ph.D.-7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml, washed, eluted with glycine buffer at pH 2.7, and immediately brought to pH 7. .. The eluate was transferred to a plate coated with monoclonal IgM and incubated according to the same protocol, but this time, the phage solution was collected after adsorption and amplified once, following the procedure described by Matochko et al. ( ).

    Expressing:

    Article Title: Amsacta moorei Entomopoxvirus Inhibitor of Apoptosis Suppresses Cell Death by Binding Grim and Hid
    Article Snippet: .. A phage library (Ph.D.-7 Phage Display Peptide Library kit; New England BioLabs) expressing seven random amino acids fused to the amino terminus of the mature PIII coat protein was used for screening. .. For each GST-BIR protein, three sequential round of affinity purification and reamplification of the phage were carried out, with ≥20 phage isolates sequenced after each round of selection.

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    New England Biolabs ph d 7 phage display peptide library kit
    Distribution of the amino acid residues in the mimotope library. (A) Log odds (LO) relative to background frequencies. (B) Sequence logo of the LO by position relative to the overall background frequencies. (C) Sequence logo of the frequencies by position. (D) Sequence logo of the LO by position in an amplified <t>Ph.D.-7</t> phage library without ligand selection relative to the overall background frequencies (based on W. L. Matochko, R. Derda. “Error analysis of deep sequencing of phage libraries: peptides censored in sequencing”. Comput Math Methods Med , 2013, 491612. 2013., http://www.chem.ualberta.ca/~derda/parasitepaper/rawfiles/PhD7-GAC-30FuR.txt ). (E) Sequence logo of the LO by position relative to the frequencies by position in the amplified, unselected library shown in (D) . The skewing of the distribution in the free N-terminus appears to be property of the library, whereas the selection by the IgM repertoire leads to slight skewing in the middle of the sequence toward negatively charged residues, glycine and tryptophan.
    Ph D 7 Phage Display Peptide Library Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ph d 7 phage display peptide library kit/product/New England Biolabs
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ph d 7 phage display peptide library kit - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Distribution of the amino acid residues in the mimotope library. (A) Log odds (LO) relative to background frequencies. (B) Sequence logo of the LO by position relative to the overall background frequencies. (C) Sequence logo of the frequencies by position. (D) Sequence logo of the LO by position in an amplified Ph.D.-7 phage library without ligand selection relative to the overall background frequencies (based on W. L. Matochko, R. Derda. “Error analysis of deep sequencing of phage libraries: peptides censored in sequencing”. Comput Math Methods Med , 2013, 491612. 2013., http://www.chem.ualberta.ca/~derda/parasitepaper/rawfiles/PhD7-GAC-30FuR.txt ). (E) Sequence logo of the LO by position relative to the frequencies by position in the amplified, unselected library shown in (D) . The skewing of the distribution in the free N-terminus appears to be property of the library, whereas the selection by the IgM repertoire leads to slight skewing in the middle of the sequence toward negatively charged residues, glycine and tryptophan.

    Journal: Frontiers in Immunology

    Article Title: Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries

    doi: 10.3389/fimmu.2019.02796

    Figure Lengend Snippet: Distribution of the amino acid residues in the mimotope library. (A) Log odds (LO) relative to background frequencies. (B) Sequence logo of the LO by position relative to the overall background frequencies. (C) Sequence logo of the frequencies by position. (D) Sequence logo of the LO by position in an amplified Ph.D.-7 phage library without ligand selection relative to the overall background frequencies (based on W. L. Matochko, R. Derda. “Error analysis of deep sequencing of phage libraries: peptides censored in sequencing”. Comput Math Methods Med , 2013, 491612. 2013., http://www.chem.ualberta.ca/~derda/parasitepaper/rawfiles/PhD7-GAC-30FuR.txt ). (E) Sequence logo of the LO by position relative to the frequencies by position in the amplified, unselected library shown in (D) . The skewing of the distribution in the free N-terminus appears to be property of the library, whereas the selection by the IgM repertoire leads to slight skewing in the middle of the sequence toward negatively charged residues, glycine and tryptophan.

    Article Snippet: A 7-mer random peptide library (E8100S, Ph.D.-7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml, washed, eluted with glycine buffer at pH 2.7, and immediately brought to pH 7.

    Techniques: Sequencing, Amplification, Selection

    Phage display sequences identified after three rounds of phage selection and amplification. (A) Twenty representative isolates from the Ph.D.-7 library (New England BioLabs) were sequenced after each round. Recurrences of specific amino acids after the third round are indicated in parentheses. X, no significant recurrence. (B) The binding motif sequences of mammalian (human) and insect ( Drosophila ) IBM-containing proteins. The first seven amino acids of the mature forms of each protein are shown. Superscripted numbers indicate the amino acid positions of the alanine in the precursor proteins.

    Journal: Journal of Virology

    Article Title: Amsacta moorei Entomopoxvirus Inhibitor of Apoptosis Suppresses Cell Death by Binding Grim and Hid

    doi: 10.1128/JVI.79.6.3684-3691.2005

    Figure Lengend Snippet: Phage display sequences identified after three rounds of phage selection and amplification. (A) Twenty representative isolates from the Ph.D.-7 library (New England BioLabs) were sequenced after each round. Recurrences of specific amino acids after the third round are indicated in parentheses. X, no significant recurrence. (B) The binding motif sequences of mammalian (human) and insect ( Drosophila ) IBM-containing proteins. The first seven amino acids of the mature forms of each protein are shown. Superscripted numbers indicate the amino acid positions of the alanine in the precursor proteins.

    Article Snippet: A phage library (Ph.D.-7 Phage Display Peptide Library kit; New England BioLabs) expressing seven random amino acids fused to the amino terminus of the mature PIII coat protein was used for screening.

    Techniques: Selection, Amplification, Binding Assay

    Identification of LPS specific peptide mimotopes. LPS antibody was immobilized at a concentration of 1 µg per well of a 96-well plate. Phages (2×10 11 ) expressing 7-mer peptides were initially added to the well of a 96-well plate containing immobilized LPS antibody after which non-specific phages were removed. Twelve random phage clones were selected from three rounds of panning and specificity to LPS antibody (black bars) was confirmed by ELISA using HSP70 antibody (white bars) as a negative control. All 12 (RS01-RS12) clones selected displayed specific reactivity to LPS antibody, as determined using the HRP labeled anti-M13 phage antibody which was detected by the HRP substrate SIGMAFAST™ OPD and absorbance measured at 490 nm. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.

    Journal: PLoS ONE

    Article Title: Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants

    doi: 10.1371/journal.pone.0030839

    Figure Lengend Snippet: Identification of LPS specific peptide mimotopes. LPS antibody was immobilized at a concentration of 1 µg per well of a 96-well plate. Phages (2×10 11 ) expressing 7-mer peptides were initially added to the well of a 96-well plate containing immobilized LPS antibody after which non-specific phages were removed. Twelve random phage clones were selected from three rounds of panning and specificity to LPS antibody (black bars) was confirmed by ELISA using HSP70 antibody (white bars) as a negative control. All 12 (RS01-RS12) clones selected displayed specific reactivity to LPS antibody, as determined using the HRP labeled anti-M13 phage antibody which was detected by the HRP substrate SIGMAFAST™ OPD and absorbance measured at 490 nm. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.

    Article Snippet: Phage Display Identification of the LPS peptide mimics was performed by using a 7-mer Phage display peptide library referred to as Ph.D.-7 (New England BioLabs, Ipswich, MA).

    Techniques: Concentration Assay, Expressing, Clone Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Labeling, Standard Deviation

    ( a ) Phage panning procedure. The Ph.D.-7 phage display library was co-incubated with AAV capsids to allow binding (B), followed by washing (W) of unbound phage and elution (E) of bound phage. Bound phage were then amplified and subjected to three rounds

    Journal: Gene therapy

    Article Title: Peptide affinity reagents for AAV capsid recognition and purification

    doi: 10.1038/gt.2011.46

    Figure Lengend Snippet: ( a ) Phage panning procedure. The Ph.D.-7 phage display library was co-incubated with AAV capsids to allow binding (B), followed by washing (W) of unbound phage and elution (E) of bound phage. Bound phage were then amplified and subjected to three rounds

    Article Snippet: Phage panning studies were carried out using the Ph.D.-7 peptide library kit (New England Biolabs) with a diversity of ~1.2×109 linear heptapeptides as per manufacturer instructions.

    Techniques: Incubation, Binding Assay, Amplification