anti m13 pill monoclonal antibody  (New England Biolabs)


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    Name:
    Anti M13 plll Monoclonal Antibody
    Description:
    Anti M13 plll Monoclonal Antibody 0 1 ml
    Catalog Number:
    e8033s
    Price:
    284
    Size:
    0 1 ml
    Category:
    Primary Antibodies
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    Structured Review

    New England Biolabs anti m13 pill monoclonal antibody
    Anti M13 plll Monoclonal Antibody
    Anti M13 plll Monoclonal Antibody 0 1 ml
    https://www.bioz.com/result/anti m13 pill monoclonal antibody/product/New England Biolabs
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    anti m13 pill monoclonal antibody - by Bioz Stars, 2020-07
    93/100 stars

    Images

    1) Product Images from "Identification of peptide targets in neuromyelitis optica"

    Article Title: Identification of peptide targets in neuromyelitis optica

    Journal: Journal of neuroimmunology

    doi: 10.1016/j.jneuroim.2011.04.007

    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Figure Legend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    2) Product Images from "Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1"

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0042344

    Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.
    Figure Legend Snippet: Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Techniques Used: Western Blot, Recombinant, Expressing, Migration

    3) Product Images from "Identification of peptide targets in neuromyelitis optica"

    Article Title: Identification of peptide targets in neuromyelitis optica

    Journal: Journal of neuroimmunology

    doi: 10.1016/j.jneuroim.2011.04.007

    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Figure Legend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    Related Articles

    Clone Assay:

    Article Title: A novel FGFR1-binding peptide exhibits anti-tumor effect on lung cancer by inhibiting proliferation and angiogenesis
    Article Snippet: .. Phage clones (2×1011 pfu/well) and control phage vcsM13 were incubated at room temperature (RT) for 1 h. For competitive elution, 2 µg/ml FGF2 (PeproTech) was added for incubation at RT for 1 h. Anti-M13 monoclonal antibody (New England Biolabs) was added and incubated at 37°C for 1 h. Next goat anti-mouse immunoglobulin G (IgG)-HRP was added and incubated at 37°C for 30 min. ..

    Article Title: A novel FGFR1-binding peptide exhibits anti-tumor effect on lung cancer by inhibiting proliferation and angiogenesis
    Article Snippet: .. ELISA assay for selected phage clones Phage clones (2×1011 pfu/well) and control phage vcsM13 were incubated at room temperature (RT) for 1 h. For competitive elution, 2 µg/ml FGF2 (PeproTech) was added for incubation at RT for 1 h. Anti-M13 monoclonal antibody (New England Biolabs) was added and incubated at 37°C for 1 h. Next goat anti-mouse immunoglobulin G (IgG)-HRP was added and incubated at 37°C for 30 min. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A novel FGFR1-binding peptide exhibits anti-tumor effect on lung cancer by inhibiting proliferation and angiogenesis
    Article Snippet: .. ELISA assay for selected phage clones Phage clones (2×1011 pfu/well) and control phage vcsM13 were incubated at room temperature (RT) for 1 h. For competitive elution, 2 µg/ml FGF2 (PeproTech) was added for incubation at RT for 1 h. Anti-M13 monoclonal antibody (New England Biolabs) was added and incubated at 37°C for 1 h. Next goat anti-mouse immunoglobulin G (IgG)-HRP was added and incubated at 37°C for 30 min. ..

    Incubation:

    Article Title: Identification of peptide targets in neuromyelitis optica
    Article Snippet: .. For detection of phage pill protein, duplicate filters were incubated with a 1:25,000 dilution of anti-M13 pill monoclonal antibody (New England BioLabs). .. Anti-M13 pill monoclonal antibody recognizes nonreduced and denatured reduced forms of bacteriophage M13 minor coat protein pill or pill fusions with peptides.

    Article Title: A novel FGFR1-binding peptide exhibits anti-tumor effect on lung cancer by inhibiting proliferation and angiogenesis
    Article Snippet: .. Phage clones (2×1011 pfu/well) and control phage vcsM13 were incubated at room temperature (RT) for 1 h. For competitive elution, 2 µg/ml FGF2 (PeproTech) was added for incubation at RT for 1 h. Anti-M13 monoclonal antibody (New England Biolabs) was added and incubated at 37°C for 1 h. Next goat anti-mouse immunoglobulin G (IgG)-HRP was added and incubated at 37°C for 30 min. ..

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

    Article Title: A novel FGFR1-binding peptide exhibits anti-tumor effect on lung cancer by inhibiting proliferation and angiogenesis
    Article Snippet: .. ELISA assay for selected phage clones Phage clones (2×1011 pfu/well) and control phage vcsM13 were incubated at room temperature (RT) for 1 h. For competitive elution, 2 µg/ml FGF2 (PeproTech) was added for incubation at RT for 1 h. Anti-M13 monoclonal antibody (New England Biolabs) was added and incubated at 37°C for 1 h. Next goat anti-mouse immunoglobulin G (IgG)-HRP was added and incubated at 37°C for 30 min. ..

    other:

    Article Title: Identification of peptide targets in neuromyelitis optica
    Article Snippet: Anti-M13 pill monoclonal antibody recognizes nonreduced and denatured reduced forms of bacteriophage M13 minor coat protein pill or pill fusions with peptides.

    Western Blot:

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

    Binding Assay:

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

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  • 93
    New England Biolabs anti m13 pill monoclonal antibody
    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti <t>M13</t> antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Anti M13 Pill Monoclonal Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti m13 pill monoclonal antibody/product/New England Biolabs
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti m13 pill monoclonal antibody - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Journal: Journal of neuroimmunology

    Article Title: Identification of peptide targets in neuromyelitis optica

    doi: 10.1016/j.jneuroim.2011.04.007

    Figure Lengend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Article Snippet: Anti-M13 pill monoclonal antibody recognizes nonreduced and denatured reduced forms of bacteriophage M13 minor coat protein pill or pill fusions with peptides.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Journal: PLoS ONE

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1

    doi: 10.1371/journal.pone.0042344

    Figure Lengend Snippet: Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Article Snippet: For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton.

    Techniques: Western Blot, Recombinant, Expressing, Migration