anti m13 pill monoclonal antibody  (New England Biolabs)


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    Name:
    Anti M13 plll Monoclonal Antibody
    Description:
    Anti M13 plll Monoclonal Antibody 0 1 ml
    Catalog Number:
    E8033S
    Price:
    284
    Category:
    Primary Antibodies
    Size:
    0 1 ml
    Buy from Supplier


    Structured Review

    New England Biolabs anti m13 pill monoclonal antibody
    Anti M13 plll Monoclonal Antibody
    Anti M13 plll Monoclonal Antibody 0 1 ml
    https://www.bioz.com/result/anti m13 pill monoclonal antibody/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti m13 pill monoclonal antibody - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Identification of peptide targets in neuromyelitis optica"

    Article Title: Identification of peptide targets in neuromyelitis optica

    Journal: Journal of neuroimmunology

    doi: 10.1016/j.jneuroim.2011.04.007

    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Figure Legend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    2) Product Images from "Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1"

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0042344

    Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.
    Figure Legend Snippet: Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Techniques Used: Western Blot, Recombinant, Expressing, Migration

    3) Product Images from "Phage Ligands for Identification of Mesenchymal-Like Breast Cancer Cells and Cancer-Associated Fibroblasts"

    Article Title: Phage Ligands for Identification of Mesenchymal-Like Breast Cancer Cells and Cancer-Associated Fibroblasts

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2018.00625

    Ex vivo phage binding to human breast cancer tissue microarray. Tissue microarray of invasive ductal carcinoma and adjacent normal tissues were incubated with 10 10 pfu of LGLRGSL phage or Vimentin antibody and then subsequently with M13-pIII antibody for phage and secondary peroxidase antibody for phage and Vimentin, imaged with a digital light microscope. Strong brown staining of the phage and Vimentin was observed in invasive ductal carcinoma sections (A–C) and metastasis in lymph nodes, (D) while no staining was observed in normal breast tissue, (E) Scale Bar is 40 and 10 μm for the inserts.
    Figure Legend Snippet: Ex vivo phage binding to human breast cancer tissue microarray. Tissue microarray of invasive ductal carcinoma and adjacent normal tissues were incubated with 10 10 pfu of LGLRGSL phage or Vimentin antibody and then subsequently with M13-pIII antibody for phage and secondary peroxidase antibody for phage and Vimentin, imaged with a digital light microscope. Strong brown staining of the phage and Vimentin was observed in invasive ductal carcinoma sections (A–C) and metastasis in lymph nodes, (D) while no staining was observed in normal breast tissue, (E) Scale Bar is 40 and 10 μm for the inserts.

    Techniques Used: Ex Vivo, Binding Assay, Microarray, Incubation, Light Microscopy, Staining

    4) Product Images from "Identification of peptide targets in neuromyelitis optica"

    Article Title: Identification of peptide targets in neuromyelitis optica

    Journal: Journal of neuroimmunology

    doi: 10.1016/j.jneuroim.2011.04.007

    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Figure Legend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    Related Articles

    other:

    Article Title: Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners
    Article Snippet: Antibodies Anti-M13 fused to HRP (phage display library kit, New England Biolabs), anti-CAS (BD Transduction Laboratories, mouse monoclonal clone 21), anti-GST (G7781, Sigma, rabbit polyclonal), anti-FLAG (Sigma, mouse monoclonal clone M2), anti-FAK (C–20, Santa Cruz Biotechnology, rabbit), anti v-Src (Ab–1, Calibiochem, mouse monoclonal clone 327), anti-Vinculin (Sigma, mouse monoclonal clone V284), anti-CAS pY165 (#4015, Cell Signaling Technology, rabbit polyclonal), anti-Src pY418 (#2101, Cell Signaling Technology, rabbit polyclonal), anti-Actin (C–11, Santa Cruz, goat polyclonal), and secondary antibody/ies fused to HRP (Abcam) were used as purchased.

    Article Title: Identification of peptide targets in neuromyelitis optica
    Article Snippet: Anti-M13 pill monoclonal antibody recognizes nonreduced and denatured reduced forms of bacteriophage M13 minor coat protein pill or pill fusions with peptides.

    Incubation:

    Article Title: Identification of peptide targets in neuromyelitis optica
    Article Snippet: The membrane was incubated with SuperSignal® West Femto Maximum Sensitivity chemiluminescent substrate (Pierce) as recommended by the manufacturer. .. For detection of phage pill protein, duplicate filters were incubated with a 1:25,000 dilution of anti-M13 pill monoclonal antibody (New England BioLabs). .. Anti-M13 pill monoclonal antibody recognizes nonreduced and denatured reduced forms of bacteriophage M13 minor coat protein pill or pill fusions with peptides.

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: 1011 phage particles diluted in 16 µl of loading buffer were boiled 5 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

    Article Title: Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF
    Article Snippet: The membrane was incubated with SuperSignal® West Femto Maximum Sensitivity chemiluminescent substrate (Pierce) as recommended by the manufacturer. .. For detection of phage pIII protein, duplicate filters were incubated with a 1:25,000 dilution of anti-M13 pIII monoclonal antibody (New England BioLabs). .. A 1:25,000 dilution of goat anti-mouse IgG peroxidase conjugate (Vector lab) was used as secondary antibody followed by chemiluminescent detection with Immobilon (Millipore).

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: 1011 phage particles diluted in 16 μl of loading buffer were boiled 3 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer and detected by conventional silver staining. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Western Blot:

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: 1011 phage particles diluted in 16 µl of loading buffer were boiled 5 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: 1011 phage particles diluted in 16 μl of loading buffer were boiled 3 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer and detected by conventional silver staining. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Binding Assay:

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1
    Article Snippet: 1011 phage particles diluted in 16 µl of loading buffer were boiled 5 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton. .. Then the membranes were washed several times and incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) diluted 1∶2000 in PBS/milk/triton.

    Article Title: AMYLOID-? PEPTIDE BINDS TO MICROTUBULE-ASSOCIATED PROTEIN 1B (MAP1B)
    Article Snippet: 1011 phage particles diluted in 16 μl of loading buffer were boiled 3 minutes and separated on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature as recommended by manufacturer and detected by conventional silver staining. .. For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Invitrogen) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) or HRP-conjugated anti-E-tag antibody (Amersham) diluted 1:1000 in PBS/milk/triton. .. The membranes incubated with anti-M13 pIII antibody were washed several times and then incubated for 2 hrs at room temperature in PBS/milk/triton containing the HPR-conjugated anti-mouse IgG2a secondary antibody (Zymed) at a dilution of 1:2000.

    Recombinant:

    Article Title: Inhibitory effect of midkine-binding peptide on tumor proliferation and migration
    Article Snippet: .. Recombinant human midkine (rh-midkine) was purchased from BioVendor Laboratory Medicine Inc. Anti-M13 phage antibody was purchased from New England Biolabs Inc. (Beverly, MA, USA). .. Anti-factor VIII antibody was purchased from DAKO (Denmark).

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    New England Biolabs anti m13 pill monoclonal antibody
    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti <t>M13</t> antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage
    Anti M13 Pill Monoclonal Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti m13 pill monoclonal antibody/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti m13 pill monoclonal antibody - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results


    Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Journal: Journal of neuroimmunology

    Article Title: Identification of peptide targets in neuromyelitis optica

    doi: 10.1016/j.jneuroim.2011.04.007

    Figure Lengend Snippet: Competitive inhibition ELISA confirms binding specificity of phage peptides. Phage peptides (−1 × 10 10 ) were added to ELISA wells coated with anti M13 antibody, NMO rAb (10 μg/ml) pre-incubated with serial 2-fold dilutions of phage

    Article Snippet: For detection of phage pill protein, duplicate filters were incubated with a 1:25,000 dilution of anti-M13 pill monoclonal antibody (New England BioLabs).

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Journal: PLoS ONE

    Article Title: Amyloid-? Peptide Binds to Cytochrome C Oxidase Subunit 1

    doi: 10.1371/journal.pone.0042344

    Figure Lengend Snippet: Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1. 10 11 phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.

    Article Snippet: For Western Blot analysis, peptides were electrophoretically transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) and the membranes were incubated for 1 h at room temperature in PBS containing 2% non-fat dry milk and 0.2% Triton X-100 (PBS/milk/triton) to eliminate non-specific binding followed by overnight incubation at 4°C with anti-M13 pIII monoclonal antibody (New England Biolabs, MA, USA) diluted 1∶1000 in PBS/milk/triton.

    Techniques: Western Blot, Recombinant, Expressing, Migration

    Ex vivo phage binding to human breast cancer tissue microarray. Tissue microarray of invasive ductal carcinoma and adjacent normal tissues were incubated with 10 10 pfu of LGLRGSL phage or Vimentin antibody and then subsequently with M13-pIII antibody for phage and secondary peroxidase antibody for phage and Vimentin, imaged with a digital light microscope. Strong brown staining of the phage and Vimentin was observed in invasive ductal carcinoma sections (A–C) and metastasis in lymph nodes, (D) while no staining was observed in normal breast tissue, (E) Scale Bar is 40 and 10 μm for the inserts.

    Journal: Frontiers in Oncology

    Article Title: Phage Ligands for Identification of Mesenchymal-Like Breast Cancer Cells and Cancer-Associated Fibroblasts

    doi: 10.3389/fonc.2018.00625

    Figure Lengend Snippet: Ex vivo phage binding to human breast cancer tissue microarray. Tissue microarray of invasive ductal carcinoma and adjacent normal tissues were incubated with 10 10 pfu of LGLRGSL phage or Vimentin antibody and then subsequently with M13-pIII antibody for phage and secondary peroxidase antibody for phage and Vimentin, imaged with a digital light microscope. Strong brown staining of the phage and Vimentin was observed in invasive ductal carcinoma sections (A–C) and metastasis in lymph nodes, (D) while no staining was observed in normal breast tissue, (E) Scale Bar is 40 and 10 μm for the inserts.

    Article Snippet: Slides were then treated with M13-pIII phage monoclonal antibody (NEB, MA) or Vimentin antibody (Cell Siganling, Danvers, MA, United States) (1:100) and then subsequently with HRP conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch Laboratories Inc., West Grove, PA, United States) for 40 min.

    Techniques: Ex Vivo, Binding Assay, Microarray, Incubation, Light Microscopy, Staining