amylose resin  (New England Biolabs)


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  • 99
    Name:
    Amylose Resin
    Description:
    Amylose Resin 100 ml
    Catalog Number:
    E8021L
    Price:
    1158
    Category:
    Protein Purification Kit Components
    Size:
    100 ml
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    Structured Review

    New England Biolabs amylose resin
    Amylose Resin
    Amylose Resin 100 ml
    https://www.bioz.com/result/amylose resin/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amylose resin - by Bioz Stars, 2021-07
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Escherichia coli Proteome Microarrays Identified the Substrates of ClpYQ Protease *
    Article Snippet: A QCM (Affinix QN μ) was employed to measure the Kd values of the YbaB-ClpY protein interactions, which were expressed in the transformed E. coli cells through pMal- ybaB and pET21a (6x his tag)- clpY ( ). .. The MBP-YbaB and His-ClpY were purified using amylose resin (New England Biolabs, NEB, Ipswich, MA) and Nickel resin (Talon resin, Takara, Kusatsu, Shiga, Japan), respectively. ..

    Article Title: Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice
    Article Snippet: Plasmid construction and protein expression Mouse CCP6, ubiquitin (Ub), Ub(K48R), and Ub(K63R) were cloned into pCDNA4-MycHis expression vector. .. CCP6-H230S/E233Q mutant (CCP6mut) and CCP6wt were also subcloned into H-MBP-3C vector and purified using amylose resin (New England BioLabs, Ipswich, USA) according to the manufacturer’s instruction. .. CCP1, TTLL4, Cullin1, and Skp1 were cloned into p3×flag-CMV-9 expression vector. pMX5-Oct4, pMX5-Sox2, pMX5-Klf4, and pMX5-cMyc plasmids were from Dr. Yang Xu’s lab (University of California, San Diego, La Jolla, USA). pDEST-Flag-PHF13-βTrCP1 plasmid was from Dr. Degui Chen’s lab (Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China).

    Mutagenesis:

    Article Title: Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice
    Article Snippet: Plasmid construction and protein expression Mouse CCP6, ubiquitin (Ub), Ub(K48R), and Ub(K63R) were cloned into pCDNA4-MycHis expression vector. .. CCP6-H230S/E233Q mutant (CCP6mut) and CCP6wt were also subcloned into H-MBP-3C vector and purified using amylose resin (New England BioLabs, Ipswich, USA) according to the manufacturer’s instruction. .. CCP1, TTLL4, Cullin1, and Skp1 were cloned into p3×flag-CMV-9 expression vector. pMX5-Oct4, pMX5-Sox2, pMX5-Klf4, and pMX5-cMyc plasmids were from Dr. Yang Xu’s lab (University of California, San Diego, La Jolla, USA). pDEST-Flag-PHF13-βTrCP1 plasmid was from Dr. Degui Chen’s lab (Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China).

    Plasmid Preparation:

    Article Title: Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice
    Article Snippet: Plasmid construction and protein expression Mouse CCP6, ubiquitin (Ub), Ub(K48R), and Ub(K63R) were cloned into pCDNA4-MycHis expression vector. .. CCP6-H230S/E233Q mutant (CCP6mut) and CCP6wt were also subcloned into H-MBP-3C vector and purified using amylose resin (New England BioLabs, Ipswich, USA) according to the manufacturer’s instruction. .. CCP1, TTLL4, Cullin1, and Skp1 were cloned into p3×flag-CMV-9 expression vector. pMX5-Oct4, pMX5-Sox2, pMX5-Klf4, and pMX5-cMyc plasmids were from Dr. Yang Xu’s lab (University of California, San Diego, La Jolla, USA). pDEST-Flag-PHF13-βTrCP1 plasmid was from Dr. Degui Chen’s lab (Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China).

    Incubation:

    Article Title: Structure of a mitochondrial fission dynamin in the closed conformation
    Article Snippet: .. The supernatant was incubated with amylose resin (New England BioLabs) for 3 hrs at 4 °C with gentle stirring, and loaded into a gravity column. ..

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  • 99
    New England Biolabs 1cv amylose resin
    1cv Amylose Resin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1cv amylose resin/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1cv amylose resin - by Bioz Stars, 2021-07
    99/100 stars
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