ultra ii rna first strand synthesis module  (New England Biolabs)


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    Name:
    NEBNext Ultra II RNA First Strand Synthesis Module
    Description:
    NEBNext Ultra II RNA First Strand Synthesis Module 96 rxns
    Catalog Number:
    e7771l
    Price:
    480
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    Structured Review

    New England Biolabs ultra ii rna first strand synthesis module
    NEBNext Ultra II RNA First Strand Synthesis Module
    NEBNext Ultra II RNA First Strand Synthesis Module 96 rxns
    https://www.bioz.com/result/ultra ii rna first strand synthesis module/product/New England Biolabs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultra ii rna first strand synthesis module - by Bioz Stars, 2020-02
    92/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500). .. Pooled libraries were sequenced on an Illumina NextSeq 500 (2 × 150 paired-end), and the untrimmed reads are available via the NCBI SRA project PRJNA379675.

    Amplification:

    Article Title: Bioorthogonal metabolic labeling of nascent RNA in neurons improves the sensitivity of transcriptome-wide profiling
    Article Snippet: RNA samples for sequencing were converted to dscDNA using the NEBNext® Ultra™ II RNA First Strand Synthesis Module (#E7771) and NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (#E6111). .. Following conversion to dscDNA, sample libraries were prepared using the KAPA Hyper Prep Kit (#KK8504) using a 300nM adapter stock concentration (Set A) and 12-cycle amplification step (see for adapters used for each sample).

    RNA Sequencing Assay:

    Article Title: Ibrutinib Displays Atrial-Specific Toxicity in Human Stem Cell-Derived Cardiomyocytes
    Article Snippet: Paragraph title: RNA Sequencing ... Library preparation was performed following the standard protocol for the NEBnext Ultra II Stranded mRNA (New England Biolabs) and sequencing was performed on the NextSeq 500 (Ilumina) with 20 million paired-end reads.

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: These RNA-seq data (SRP103307) were developed from divergent Leucaena species to represent at least one member of each well-supported clade (genome group) within the genus , including L. cruziana (Oxford Forestry Institute seedlot 43/85/16), L. cuspidata (83/94), L. esculenta (47/87/01), L. pulverulenta (84/87/05), and L. trichandra (4/91/15), as well as the allotetraploid hybrid of L. cruziana and L. pulverulenta , L. leucocephala (80/92/02) ( ). .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500).

    Isolation:

    Article Title: Ibrutinib Displays Atrial-Specific Toxicity in Human Stem Cell-Derived Cardiomyocytes
    Article Snippet: Total RNA of hESC-CMs was isolated by Illustra RNAspin Mini Isolation Kit (GE Healthcare) after 24 h of incubation of 1 μM ibrutinib in the experimental group and vehicle (DMSO) in the control group. .. Library preparation was performed following the standard protocol for the NEBnext Ultra II Stranded mRNA (New England Biolabs) and sequencing was performed on the NextSeq 500 (Ilumina) with 20 million paired-end reads.

    Incubation:

    Article Title: Ibrutinib Displays Atrial-Specific Toxicity in Human Stem Cell-Derived Cardiomyocytes
    Article Snippet: Total RNA of hESC-CMs was isolated by Illustra RNAspin Mini Isolation Kit (GE Healthcare) after 24 h of incubation of 1 μM ibrutinib in the experimental group and vehicle (DMSO) in the control group. .. Library preparation was performed following the standard protocol for the NEBnext Ultra II Stranded mRNA (New England Biolabs) and sequencing was performed on the NextSeq 500 (Ilumina) with 20 million paired-end reads.

    Purification:

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500). .. Pooled libraries were sequenced on an Illumina NextSeq 500 (2 × 150 paired-end), and the untrimmed reads are available via the NCBI SRA project PRJNA379675.

    Concentration Assay:

    Article Title: Bioorthogonal metabolic labeling of nascent RNA in neurons improves the sensitivity of transcriptome-wide profiling
    Article Snippet: RNA samples for sequencing were converted to dscDNA using the NEBNext® Ultra™ II RNA First Strand Synthesis Module (#E7771) and NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (#E6111). .. Following conversion to dscDNA, sample libraries were prepared using the KAPA Hyper Prep Kit (#KK8504) using a 300nM adapter stock concentration (Set A) and 12-cycle amplification step (see for adapters used for each sample).

    Generated:

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: RNA-seq data generated from three biological replicates per sample (three whole seedlings harvested at the third-leaf stage of development) that were combined, extracted, sequenced, and mapped to the L. trichandra mt-genome. .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500).

    DNA Sequencing:

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: RNA editing was assessed empirically through a combination of additional RNA-seq and DNA-seq data resources. .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500).

    Sequencing:

    Article Title: Ibrutinib Displays Atrial-Specific Toxicity in Human Stem Cell-Derived Cardiomyocytes
    Article Snippet: .. Library preparation was performed following the standard protocol for the NEBnext Ultra II Stranded mRNA (New England Biolabs) and sequencing was performed on the NextSeq 500 (Ilumina) with 20 million paired-end reads. ..

    Article Title: Bioorthogonal metabolic labeling of nascent RNA in neurons improves the sensitivity of transcriptome-wide profiling
    Article Snippet: .. RNA samples for sequencing were converted to dscDNA using the NEBNext® Ultra™ II RNA First Strand Synthesis Module (#E7771) and NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (#E6111). ..

    Article Title: PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing
    Article Snippet: Each of these samples was subjected to two-independent library preps and sequencing runs (technical replicates). .. RNA was extracted using the Norgen Plant RNA/DNA Purification Kit (Norgen Corp., Toronto, CA) and treated with plant rRNA depletion reagents (courtesy of New England Biolabs) prior to library construction using the NEBNext Ultra II RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers Sets 1 & 2) (#E7335 and #E7500).

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  • 92
    New England Biolabs ultra ii rna first strand synthesis module
    Ultra Ii Rna First Strand Synthesis Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra ii rna first strand synthesis module/product/New England Biolabs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultra ii rna first strand synthesis module - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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