e7420  (New England Biolabs)


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    Name:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7420l
    Price:
    3877
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    New England Biolabs e7420
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/e7420/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e7420 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs
    Article Snippet: .. An NEBNext ultradirectional RNA library kit (New England Biolabs, catalog no. E7420S) was used to make cDNA and RNA-seq libraries. .. Several minor modifications to the standard kit protocol were made based on (1) size (RNA fragmentation time was decreased from 15 to 5 min) and (2) the small amount of RNA (the number of PCR cycles were adjusted to 20 cycles in the USER excision and PCR library step).

    Article Title: Selective release of circRNAs in platelet-derived extracellular vesicles
    Article Snippet: .. RNA-seq and data analysis For high-throughput sequencing, total RNA from platelets and microvesicles was depleted of ribosomal RNA, using the Ribo-Zero Gold rRNA Removal Kit (Epicenter, MRZG126), followed by library preparation (NEBNext Ultra Directional RNA Library Prep Kit, NEB, E7420, according to the manufacturer’s instructions). .. For library preparation of RNA isolated from exosomes the SMARTer® Stranded RNA-Seq Kit (Takara-Clontech) was used, according to the manufacturer’s instructions.

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification
    Article Snippet: .. Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). .. Paired-end sequencing with a read length of 150 bp was carried out on an Illumina HiSeq 4000.

    Construct:

    Article Title: Global mapping transcriptional start sites revealed both transcriptional and post-transcriptional regulation of cold adaptation in the methanogenic archaeon Methanolobus psychrophilus
    Article Snippet: .. In addition, for TSS refinement and verificaion, standard strand-specific whole-transcript sequencing cDNA libraries (w library) were constructed for the total RNAs using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer's instructions. .. High-throughput sequencing and quality control (QC) cDNA libraries were sequenced on an Illumina HiSeq 2000 platform.

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

    Purification:

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification
    Article Snippet: .. Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). .. Paired-end sequencing with a read length of 150 bp was carried out on an Illumina HiSeq 4000.

    Sequencing:

    Article Title: Global mapping transcriptional start sites revealed both transcriptional and post-transcriptional regulation of cold adaptation in the methanogenic archaeon Methanolobus psychrophilus
    Article Snippet: .. In addition, for TSS refinement and verificaion, standard strand-specific whole-transcript sequencing cDNA libraries (w library) were constructed for the total RNAs using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer's instructions. .. High-throughput sequencing and quality control (QC) cDNA libraries were sequenced on an Illumina HiSeq 2000 platform.

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

    Article Title: Selective release of circRNAs in platelet-derived extracellular vesicles
    Article Snippet: .. RNA-seq and data analysis For high-throughput sequencing, total RNA from platelets and microvesicles was depleted of ribosomal RNA, using the Ribo-Zero Gold rRNA Removal Kit (Epicenter, MRZG126), followed by library preparation (NEBNext Ultra Directional RNA Library Prep Kit, NEB, E7420, according to the manufacturer’s instructions). .. For library preparation of RNA isolated from exosomes the SMARTer® Stranded RNA-Seq Kit (Takara-Clontech) was used, according to the manufacturer’s instructions.

    Generated:

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species
    Article Snippet: .. RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany. .. RNAseq analysis Illumina 100 bp paired-end reads from four biological replicates of the four internode zones were imported into CLC genomics workbench 8 (CLCbio, Aarhus, Denmark, http://www.clcbio.com ) for adapter removal, trimming, mapping, and read counting.

    High Throughput Screening Assay:

    Article Title: Selective release of circRNAs in platelet-derived extracellular vesicles
    Article Snippet: .. RNA-seq and data analysis For high-throughput sequencing, total RNA from platelets and microvesicles was depleted of ribosomal RNA, using the Ribo-Zero Gold rRNA Removal Kit (Epicenter, MRZG126), followed by library preparation (NEBNext Ultra Directional RNA Library Prep Kit, NEB, E7420, according to the manufacturer’s instructions). .. For library preparation of RNA isolated from exosomes the SMARTer® Stranded RNA-Seq Kit (Takara-Clontech) was used, according to the manufacturer’s instructions.

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    New England Biolabs nebnext ultra directional rna library prep kit for illumina
    Schematic overview of Hi-GRIL-seq. Induction of T4 <t>RNA</t> ligase expression from the P tac promoter with IPTG leads to the expression of the enzyme and the formation of chimeras between base paired endogenous sRNAs and their targets. Following isolation of total RNA and rRNA depletion, a cDNA library for <t>Illumina</t> sequencing is constructed and sequenced. RNA interactions between sRNAs and their targets are identified by a BLAST-based analysis pipeline. Global chimeras are visualized in a two-dimensional dot plot, in which the location of the dot represents the genomic coordinate of the participating RNAs. To examine the targets of a particular RNA, the coverage of its targets can be visualized. To further zoom in on a particular interaction between the two RNAs, the exact location of ligation junctions in the chimeras are mapped and visualized.
    Nebnext Ultra Directional Rna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra directional rna library prep kit for illumina/product/New England Biolabs
    Average 99 stars, based on 1109 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra directional rna library prep kit for illumina - by Bioz Stars, 2020-09
    99/100 stars
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    Schematic overview of Hi-GRIL-seq. Induction of T4 RNA ligase expression from the P tac promoter with IPTG leads to the expression of the enzyme and the formation of chimeras between base paired endogenous sRNAs and their targets. Following isolation of total RNA and rRNA depletion, a cDNA library for Illumina sequencing is constructed and sequenced. RNA interactions between sRNAs and their targets are identified by a BLAST-based analysis pipeline. Global chimeras are visualized in a two-dimensional dot plot, in which the location of the dot represents the genomic coordinate of the participating RNAs. To examine the targets of a particular RNA, the coverage of its targets can be visualized. To further zoom in on a particular interaction between the two RNAs, the exact location of ligation junctions in the chimeras are mapped and visualized.

    Journal: Molecular microbiology

    Article Title: Probing the sRNA regulatory landscape of P. aeruginosa: post-transcriptional control of determinants of pathogenicity and antibiotic susceptibility

    doi: 10.1111/mmi.13857

    Figure Lengend Snippet: Schematic overview of Hi-GRIL-seq. Induction of T4 RNA ligase expression from the P tac promoter with IPTG leads to the expression of the enzyme and the formation of chimeras between base paired endogenous sRNAs and their targets. Following isolation of total RNA and rRNA depletion, a cDNA library for Illumina sequencing is constructed and sequenced. RNA interactions between sRNAs and their targets are identified by a BLAST-based analysis pipeline. Global chimeras are visualized in a two-dimensional dot plot, in which the location of the dot represents the genomic coordinate of the participating RNAs. To examine the targets of a particular RNA, the coverage of its targets can be visualized. To further zoom in on a particular interaction between the two RNAs, the exact location of ligation junctions in the chimeras are mapped and visualized.

    Article Snippet: After rRNA depletion or sRNA enrichment, cDNA libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Expressing, Isolation, cDNA Library Assay, Sequencing, Construct, Ligation

    RNA-seq analysis of BioTAP-XL pull-downs. ( A ) Enrichment of repeat-derived RNA in HP1a-BioTAP cross-linked complexes from S2 cells compared with MSL3-BioTAP complexes from S2 cells detected using a random-priming approach for cDNA synthesis and Illumina

    Journal: Genes & Development

    Article Title: Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs

    doi: 10.1101/gad.241950.114

    Figure Lengend Snippet: RNA-seq analysis of BioTAP-XL pull-downs. ( A ) Enrichment of repeat-derived RNA in HP1a-BioTAP cross-linked complexes from S2 cells compared with MSL3-BioTAP complexes from S2 cells detected using a random-priming approach for cDNA synthesis and Illumina

    Article Snippet: An NEBNext ultradirectional RNA library kit (New England Biolabs, catalog no. E7420S) was used to make cDNA and RNA-seq libraries.

    Techniques: RNA Sequencing Assay, Derivative Assay

    Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Journal: Biotechnology for Biofuels

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species

    doi: 10.1186/s13068-016-0457-6

    Figure Lengend Snippet: Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Article Snippet: RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany.

    Techniques: RNA Sequencing Assay

    Antisense Illumina RNA-seq reads at RCA are likely to be spurious.

    Journal: eLife

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

    doi: 10.7554/eLife.49658

    Figure Lengend Snippet: Antisense Illumina RNA-seq reads at RCA are likely to be spurious.

    Article Snippet: Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: RNA Sequencing Assay

    Changes in RNA 3′ end formation in the vir-1 mutant. ( A ) Splicing is moderately disrupted in the vir-1 mutant. Results of differential exon usage analysis with DEXseq are shown for contiguous regions (‘exon chunks’), which occur in the same sets of transcripts in the Araport11 reference. Regions were classified as a 5′ or 3′ variation if they were bounded by the TSS of one or more transcripts. Orange, features with increased usage; blue, features with reduced usage. Boxplots show the distribution in absolute log 2 fold change for each feature set. ( B ) DaPars analysis of Illumina RNAseq data indicates global 3’UTR shortening in the vir-1 mutant. Histogram showing the change in the percentage of distal poly(A) site usage in vir-1 compared to VIRc. Only genes which are significantly differentially polyadenylated (FDR

    Journal: eLife

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

    doi: 10.7554/eLife.49658

    Figure Lengend Snippet: Changes in RNA 3′ end formation in the vir-1 mutant. ( A ) Splicing is moderately disrupted in the vir-1 mutant. Results of differential exon usage analysis with DEXseq are shown for contiguous regions (‘exon chunks’), which occur in the same sets of transcripts in the Araport11 reference. Regions were classified as a 5′ or 3′ variation if they were bounded by the TSS of one or more transcripts. Orange, features with increased usage; blue, features with reduced usage. Boxplots show the distribution in absolute log 2 fold change for each feature set. ( B ) DaPars analysis of Illumina RNAseq data indicates global 3’UTR shortening in the vir-1 mutant. Histogram showing the change in the percentage of distal poly(A) site usage in vir-1 compared to VIRc. Only genes which are significantly differentially polyadenylated (FDR

    Article Snippet: Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Mutagenesis

    Properties of nanopore DRS sequencing data. ( A ) Nanopore DRS identified a 12.8 kb transcript generated from the AT1G67120 gene that includes 58 exons. Blue, nanopore DRS isoform; black, Araport11 annotation. ( B ) Synthetic ERCC RNA spike-in mixes are detected in a quantitative manner. Absolute concentrations of spike-ins are plotted against counts per million (CPM) reads in log 10 scale. Blue, ERCC RNA spike-in mix 1; orange, ERCC RNA spike-in mix 2. ( C ) Overview of the sequencing and alignment characteristics of nanopore DRS data for ERCC RNA spike-ins. Left, distribution of the length fraction of each sequenced read that aligns to the ERCC RNA spike-in reference; centre, distribution of fraction of identity that matches between the sequence of the read and the ERCC RNA spike-in reference for the aligned portion of each read; right, distributions of the occurrence of insertions (black), substitutions (orange) and deletions (blue) as a proportion of the number of aligned bases in each read. ( D ) Substitution preference for each nucleotide (left to right: adenine [A], uracil [U], guanine [G], cytosine [C]). When substituted, G is replaced with A in more than 63% of its substitutions, while C is replaced by U in 73%. Conversely, U is rarely replaced with G (12%) and A is rarely substituted with C (16%). ( E ) Nucleotide representation within the ERCC RNA Spike-In reference sequences (black dots) compared with nucleotide representation within four categories from the nanopore DRS reads. Identity matches between the sequence of the read and the ERCC RNA spike-in reference (green crosses), insertions (blue pentagons), deletions (yellow stars) and substitutions (purple diamonds).G is under-represented and U is over-represented for all three categories of error (insertion, deletion and substitution) relative to the reference nucleotide distribution. C is over-represented in deletions and substitutions. A is over-represented in insertions and deletions and under-represented in substitutions. ( F ) Signals originating from the RH3 transcripts are susceptible to systematic over-splitting around exons 7–9 (highlighted using a purple dashed box), resulting in reads with apparently novel 5′ or 3′ positions. This appears only to occur at high frequency in datasets collected after May 2018 ( Supplementary file 1 ) and may result from an update to the MinKNOW software. ( G ) PIN7 antisense RNAs detected using nanopore DRS. Blue, Col-0 PIN7 sense Illumina RNAseq coverage and nanopore PIN7 sense read alignments; orange, Col-0 PIN7 antisense Illumina RNAseq coverage and nanopore PIN7 antisense read alignments; green, hen2–two mutant PIN7 sense Illumina RNAseq coverage; purple, hen2–two mutant PIN7 antisense Illumina RNAseq coverage; black, PIN7 sense RNA isoforms found in Araport11 annotation; grey, PIN7 antisense differentially expressed regions detected with DERfinder.

    Journal: eLife

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

    doi: 10.7554/eLife.49658

    Figure Lengend Snippet: Properties of nanopore DRS sequencing data. ( A ) Nanopore DRS identified a 12.8 kb transcript generated from the AT1G67120 gene that includes 58 exons. Blue, nanopore DRS isoform; black, Araport11 annotation. ( B ) Synthetic ERCC RNA spike-in mixes are detected in a quantitative manner. Absolute concentrations of spike-ins are plotted against counts per million (CPM) reads in log 10 scale. Blue, ERCC RNA spike-in mix 1; orange, ERCC RNA spike-in mix 2. ( C ) Overview of the sequencing and alignment characteristics of nanopore DRS data for ERCC RNA spike-ins. Left, distribution of the length fraction of each sequenced read that aligns to the ERCC RNA spike-in reference; centre, distribution of fraction of identity that matches between the sequence of the read and the ERCC RNA spike-in reference for the aligned portion of each read; right, distributions of the occurrence of insertions (black), substitutions (orange) and deletions (blue) as a proportion of the number of aligned bases in each read. ( D ) Substitution preference for each nucleotide (left to right: adenine [A], uracil [U], guanine [G], cytosine [C]). When substituted, G is replaced with A in more than 63% of its substitutions, while C is replaced by U in 73%. Conversely, U is rarely replaced with G (12%) and A is rarely substituted with C (16%). ( E ) Nucleotide representation within the ERCC RNA Spike-In reference sequences (black dots) compared with nucleotide representation within four categories from the nanopore DRS reads. Identity matches between the sequence of the read and the ERCC RNA spike-in reference (green crosses), insertions (blue pentagons), deletions (yellow stars) and substitutions (purple diamonds).G is under-represented and U is over-represented for all three categories of error (insertion, deletion and substitution) relative to the reference nucleotide distribution. C is over-represented in deletions and substitutions. A is over-represented in insertions and deletions and under-represented in substitutions. ( F ) Signals originating from the RH3 transcripts are susceptible to systematic over-splitting around exons 7–9 (highlighted using a purple dashed box), resulting in reads with apparently novel 5′ or 3′ positions. This appears only to occur at high frequency in datasets collected after May 2018 ( Supplementary file 1 ) and may result from an update to the MinKNOW software. ( G ) PIN7 antisense RNAs detected using nanopore DRS. Blue, Col-0 PIN7 sense Illumina RNAseq coverage and nanopore PIN7 sense read alignments; orange, Col-0 PIN7 antisense Illumina RNAseq coverage and nanopore PIN7 antisense read alignments; green, hen2–two mutant PIN7 sense Illumina RNAseq coverage; purple, hen2–two mutant PIN7 antisense Illumina RNAseq coverage; black, PIN7 sense RNA isoforms found in Araport11 annotation; grey, PIN7 antisense differentially expressed regions detected with DERfinder.

    Article Snippet: Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Sequencing, Generated, Software, Mutagenesis

    Patterns of splicing revealed using nanopore DRS. ( A ) Nanopore DRS novel splicing events can be validated using RT-PCR. Blue, RNA isoforms found using nanopore DRS; orange, Sanger sequencing products obtained using RT-PCR; black, RNA Araport11 annotation. ( B ) Splice junction classification of unannotated GU/AG splice sites found in error-corrected nanopore DRS data that also have Illumina support. Counts are plotted in log 10 scale and the exact number of splice junctions in each set is indicated. ( C ) A novel exon skipping event at exon 3 of KH domain-containing protein AT5G56140 , identified by nanopore DRS, leads to a frameshift resulting in a premature termination codon in exon 4 (orange asterisk). Blue track, nanopore DRS cleavage, blue, nanopore DRS read alignments; black, AtRTD2 annotation; orange, full novel open reading frame (ORF). ( D ) A selection of novel alternative acceptor site at XRCC (AT1G80420), identified in nanopore DRS, leads to a frameshift resulting in a premature termination codon in exon 6 (orange asterisk). Blue track, nanopore DRS cleavage, blue, nanopore DRS read alignments; black, AtRTD2 annotation; orange, full novel open reading frame (ORF). Sanger sequencing products from novel nanopore DRS splicing events – Figure 4—figure supplement 1A .

    Journal: eLife

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

    doi: 10.7554/eLife.49658

    Figure Lengend Snippet: Patterns of splicing revealed using nanopore DRS. ( A ) Nanopore DRS novel splicing events can be validated using RT-PCR. Blue, RNA isoforms found using nanopore DRS; orange, Sanger sequencing products obtained using RT-PCR; black, RNA Araport11 annotation. ( B ) Splice junction classification of unannotated GU/AG splice sites found in error-corrected nanopore DRS data that also have Illumina support. Counts are plotted in log 10 scale and the exact number of splice junctions in each set is indicated. ( C ) A novel exon skipping event at exon 3 of KH domain-containing protein AT5G56140 , identified by nanopore DRS, leads to a frameshift resulting in a premature termination codon in exon 4 (orange asterisk). Blue track, nanopore DRS cleavage, blue, nanopore DRS read alignments; black, AtRTD2 annotation; orange, full novel open reading frame (ORF). ( D ) A selection of novel alternative acceptor site at XRCC (AT1G80420), identified in nanopore DRS, leads to a frameshift resulting in a premature termination codon in exon 6 (orange asterisk). Blue track, nanopore DRS cleavage, blue, nanopore DRS read alignments; black, AtRTD2 annotation; orange, full novel open reading frame (ORF). Sanger sequencing products from novel nanopore DRS splicing events – Figure 4—figure supplement 1A .

    Article Snippet: Preparation of libraries for Illumina RNA sequencing Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Selection