singleplex  (New England Biolabs)


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  • 94
    Name:
    NEBNext Singleplex Oligos for Illumina
    Description:
    NEBNext Singleplex Oligos for Illumina 60 rxns
    Catalog Number:
    E7350L
    Price:
    243
    Size:
    60 rxns
    Category:
    DNA Analysis
    Score:
    85
    Buy from Supplier


    Structured Review

    New England Biolabs singleplex
    NEBNext Singleplex Oligos for Illumina
    NEBNext Singleplex Oligos for Illumina 60 rxns
    https://www.bioz.com/result/singleplex/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex - by Bioz Stars, 2019-12
    94/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: RNA sequencing analysis of salivary extracellular RNA
    Article Snippet: Optional stopping point: at this point cDNA library can be stored at -20°C. .. *The Universal PCR primer and Index (X) Primer are contained in the NEBNext SinglePlex (NEB #E7350) or NEBNext Multiplex (NEB #E7335 or NEB #E7500) Oligos for Illumina. .. PCR Cycling Conditions

    Amplification:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: First PCR products were purified using NucleoSpin Gel and PCR Purification Kit then amplified using Platinum SuperFi DNA Polymerase to introduce sample-specific dual-end barcodes with the following primers: M1S: 5′-(N)4-6 (XXXXXX)CAGTGGTATCAACGCAGAG-3′ and Z: 5′-(N)4-6 (XXXXXX)ATTGGGCAGCCCTGATT-3′, where XXXXXX represents sample-specific 6-nt barcode. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    RNA Sequencing Assay:

    Article Title: Cell Type-Specific Expression Profile and Signaling Requirements in Early Hematopoietic Reprogramming
    Article Snippet: The single-cell RNA-seq strategy [ ] was employed to capture the transcriptomes of 25 hematopoietic cells. .. An RNA-seq library was prepared using the protocol of NEBNext Singleplex (#E7350) oligos from Illumina. .. All reads were aligned to the mouse reference genome mm9 from UCSC using TopHat [ ].

    Polymerase Chain Reaction:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Second PCR products were purified using NucleoSpin Gel and PCR Purification Kit. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Article Title: RNA sequencing analysis of salivary extracellular RNA
    Article Snippet: Optional stopping point: at this point cDNA library can be stored at -20°C. .. *The Universal PCR primer and Index (X) Primer are contained in the NEBNext SinglePlex (NEB #E7350) or NEBNext Multiplex (NEB #E7335 or NEB #E7500) Oligos for Illumina. .. PCR Cycling Conditions

    Introduce:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: First PCR products were purified using NucleoSpin Gel and PCR Purification Kit then amplified using Platinum SuperFi DNA Polymerase to introduce sample-specific dual-end barcodes with the following primers: M1S: 5′-(N)4-6 (XXXXXX)CAGTGGTATCAACGCAGAG-3′ and Z: 5′-(N)4-6 (XXXXXX)ATTGGGCAGCCCTGATT-3′, where XXXXXX represents sample-specific 6-nt barcode. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    Purification:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Second PCR products were purified using NucleoSpin Gel and PCR Purification Kit. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: The IC50 values were calculated by using GraphPad Prism. .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Real-time Polymerase Chain Reaction:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Concentration Assay:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara). .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    Incubation:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara). .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    cDNA Library Assay:

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: The IC50 values were calculated by using GraphPad Prism. .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Expressing:

    Article Title: Cell Type-Specific Expression Profile and Signaling Requirements in Early Hematopoietic Reprogramming
    Article Snippet: Paragraph title: RNA sequencing and expression analysis ... An RNA-seq library was prepared using the protocol of NEBNext Singleplex (#E7350) oligos from Illumina.

    Sequencing:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Second PCR products were purified using NucleoSpin Gel and PCR Purification Kit. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: Paragraph title: Deep Sequencing ... A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter).

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  • 94
    New England Biolabs singleplex
    Singleplex, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    singleplex - by Bioz Stars, 2019-12
    94/100 stars
      Buy from Supplier

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