nebnext adaptors  (New England Biolabs)


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    Name:
    NEBNext Singleplex Oligos for Illumina
    Description:
    NEBNext Singleplex Oligos for Illumina 60 rxns
    Catalog Number:
    e7350l
    Price:
    243
    Size:
    60 rxns
    Category:
    DNA Analysis
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    New England Biolabs nebnext adaptors
    NEBNext Singleplex Oligos for Illumina
    NEBNext Singleplex Oligos for Illumina 60 rxns
    https://www.bioz.com/result/nebnext adaptors/product/New England Biolabs
    Average 87 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    nebnext adaptors - by Bioz Stars, 2020-01
    87/100 stars

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    Amplification:

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: Lastly, we prepared sequencing libraries from the amplified RNA, using the TruSeq Small RNA Library Preparation kit (Illumina, catalog number RS-200-0012/RS-200-0024). .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: First PCR products were purified using NucleoSpin Gel and PCR Purification Kit then amplified using Platinum SuperFi DNA Polymerase to introduce sample-specific dual-end barcodes with the following primers: M1S: 5′-(N)4-6 (XXXXXX)CAGTGGTATCAACGCAGAG-3′ and Z: 5′-(N)4-6 (XXXXXX)ATTGGGCAGCCCTGATT-3′, where XXXXXX represents sample-specific 6-nt barcode. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    Electrophoresis:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: DNA quantification was performed using a dsDNA assay (Invitrogen) on a Qubit 2.0 fluorometer (Life Technologies) and quality was assessed by electrophoresis in a 1% agarose gel. .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    Incubation:

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: In case of gDNA extracted from small regions of single tissue sections––for which we could not measure the concentration––we pooled together gDNA extracted from up to 48 regions in a single IVT reaction of 20 μl and incubated for 14 h at 37 °C. .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara). .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    Expressing:

    Article Title: Cell Type-Specific Expression Profile and Signaling Requirements in Early Hematopoietic Reprogramming
    Article Snippet: Paragraph title: RNA sequencing and expression analysis ... An RNA-seq library was prepared using the protocol of NEBNext Singleplex (#E7350) oligos from Illumina.

    High Performance Liquid Chromatography:

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: Materials Oligos were purchased from TaKaRa (Dalian, China) and purified by HPLC before use. .. Klenow fragment polymerase (KF, 3′ → 5′ exo-), Nt.BsmAI NEase, T4 Phage β -glucosyltransferase (T4-β GT), UDP-glucose, Cutsmart buffer (20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate and 0.1 mg ml–1 BSA, pH 7.9), NEB buffer 2 (10 mM Tris–HCl, 10 mM MgCl2 , 50 mM NaCl and 1 mM dithiothreitol, pH 7.9), NEB buffer 4 (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9), the NEBNext Ultra End Repair/dA-Tailing Module, the NEBNext Ultra Ligation Module and NEBNext Singleplex Oligos for Illumina were purchased from New England Biolabs (USA).

    Ligation:

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: .. Klenow fragment polymerase (KF, 3′ → 5′ exo-), Nt.BsmAI NEase, T4 Phage β -glucosyltransferase (T4-β GT), UDP-glucose, Cutsmart buffer (20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate and 0.1 mg ml–1 BSA, pH 7.9), NEB buffer 2 (10 mM Tris–HCl, 10 mM MgCl2 , 50 mM NaCl and 1 mM dithiothreitol, pH 7.9), NEB buffer 4 (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9), the NEBNext Ultra End Repair/dA-Tailing Module, the NEBNext Ultra Ligation Module and NEBNext Singleplex Oligos for Illumina were purchased from New England Biolabs (USA). .. Deoxynucleotide triphosphates (dNTPs), UNIQ-10 Spin Column Oligo DNA Purification Kits, EZ-10 Column DNA Purification Kits and 1 × phosphate buffer saline (PBS, pH 7.4) were ordered from Shanghai Sangon Biological Engineering Technology Services Co., Ltd. (Shanghai, China).

    Introduce:

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: First PCR products were purified using NucleoSpin Gel and PCR Purification Kit then amplified using Platinum SuperFi DNA Polymerase to introduce sample-specific dual-end barcodes with the following primers: M1S: 5′-(N)4-6 (XXXXXX)CAGTGGTATCAACGCAGAG-3′ and Z: 5′-(N)4-6 (XXXXXX)ATTGGGCAGCCCTGATT-3′, where XXXXXX represents sample-specific 6-nt barcode. .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    DNA Sequencing:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: Paragraph title: DNA extraction and DNA sequencing library construction ... PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    Polymerase Chain Reaction:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors. .. The library was quantified using a Qubit fluorometer and assessed by qPCR using the Illumina Library Quantification Kit (NEBNext Library Quant Kit) and a StepONE plus Real time PCR system (Applied Biosystems).

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Sonication:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors. .. The library was quantified using a Qubit fluorometer and assessed by qPCR using the Illumina Library Quantification Kit (NEBNext Library Quant Kit) and a StepONE plus Real time PCR system (Applied Biosystems).

    Recombinant:

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: In order to prevent RNA degradation, we added 20 U of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Invitrogen, catalog number 10777-019) per 20 μl of IVT reaction. .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    DNA Extraction:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: Paragraph title: DNA extraction and DNA sequencing library construction ... PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    RNA Sequencing Assay:

    Article Title: Cell Type-Specific Expression Profile and Signaling Requirements in Early Hematopoietic Reprogramming
    Article Snippet: .. An RNA-seq library was prepared using the protocol of NEBNext Singleplex (#E7350) oligos from Illumina. ..

    Isolation:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: DNA extraction and DNA sequencing library construction A monoclonal culture from one individual stem female hatched from a diapausing egg isolated from Hoya Rasa lake (HYR1 clone) was allowed to grow as described in Supplementary Method to obtain enough biomass for DNA extraction. .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    Purification:

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. Deep Sequencing A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: Materials Oligos were purchased from TaKaRa (Dalian, China) and purified by HPLC before use. .. Klenow fragment polymerase (KF, 3′ → 5′ exo-), Nt.BsmAI NEase, T4 Phage β -glucosyltransferase (T4-β GT), UDP-glucose, Cutsmart buffer (20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate and 0.1 mg ml–1 BSA, pH 7.9), NEB buffer 2 (10 mM Tris–HCl, 10 mM MgCl2 , 50 mM NaCl and 1 mM dithiothreitol, pH 7.9), NEB buffer 4 (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9), the NEBNext Ultra End Repair/dA-Tailing Module, the NEBNext Ultra Ligation Module and NEBNext Singleplex Oligos for Illumina were purchased from New England Biolabs (USA).

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: After IVT, we purified the amplified RNA with Agencourt RNAClean XP beads (Beckman Coulter, catalog number A63987) following the manufacturer’s instructions. .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    Sequencing:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors. .. To improve sequencing quality, 1% PhiX control was spiked-in.

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. Deep Sequencing A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: Paragraph title: Deep Sequencing ... A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter).

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: Lastly, we prepared sequencing libraries from the amplified RNA, using the TruSeq Small RNA Library Preparation kit (Illumina, catalog number RS-200-0012/RS-200-0024). .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    cDNA Library Assay:

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. Deep Sequencing A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Real-time Polymerase Chain Reaction:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors. .. The library was quantified using a Qubit fluorometer and assessed by qPCR using the Illumina Library Quantification Kit (NEBNext Library Quant Kit) and a StepONE plus Real time PCR system (Applied Biosystems).

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Pooled, gel-extracted libraries were then quality controlled using a Bioanalyzer High Sensitivity Kit (Agilent) and quantified by qPCR using the Library Quantification Kit/Illumina GA/ABI Prism (KAPA Biosystems) with the Illumina Internal Control (KAPA Biosystems).

    Agarose Gel Electrophoresis:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: DNA quantification was performed using a dsDNA assay (Invitrogen) on a Qubit 2.0 fluorometer (Life Technologies) and quality was assessed by electrophoresis in a 1% agarose gel. .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs). .. Concentrations for each library were determined using Qubit dsDNA BR Assay Kit (Life Technologies) and equal quantity of each library was pooled, run on a 1.5% agarose gel and 600-800 bp band was excised and extracted using the NucleoSpin Gel and PCR Purification Kit.

    dsDNA Assay:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: DNA quantification was performed using a dsDNA assay (Invitrogen) on a Qubit 2.0 fluorometer (Life Technologies) and quality was assessed by electrophoresis in a 1% agarose gel. .. PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors.

    Concentration Assay:

    Article Title: Genomic signatures of local adaptation to the degree of environmental predictability in rotifers
    Article Snippet: PCR-free DNA libraries were prepared with the sonicated DNA using the NEBNext Ultra DNA library Prep kit for Illumina combined with NEBNext Singleplex Adaptors. .. The template DNA was denatured according to the protocol described in the Illumina system guide and loaded at 20 pM concentration.

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
    Article Snippet: In case of gDNA extracted from small regions of single tissue sections––for which we could not measure the concentration––we pooled together gDNA extracted from up to 48 regions in a single IVT reaction of 20 μl and incubated for 14 h at 37 °C. .. For CUTseq validation, we prepared libraries with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog number E7645/E7103) and NEBNext adaptors (NEB, catalog number E7350), following the manufacturer’s instructions.

    Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
    Article Snippet: Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara). .. For each sample, 300ng of purified second PCR product was used for individual sequencing library prep using NEBNext Ultra II DNA Library Prep Kit with NEBNext Singleplex Oligos both for Illumina (New England Biolabs).

    DNA Purification:

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: Klenow fragment polymerase (KF, 3′ → 5′ exo-), Nt.BsmAI NEase, T4 Phage β -glucosyltransferase (T4-β GT), UDP-glucose, Cutsmart buffer (20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate and 0.1 mg ml–1 BSA, pH 7.9), NEB buffer 2 (10 mM Tris–HCl, 10 mM MgCl2 , 50 mM NaCl and 1 mM dithiothreitol, pH 7.9), NEB buffer 4 (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9), the NEBNext Ultra End Repair/dA-Tailing Module, the NEBNext Ultra Ligation Module and NEBNext Singleplex Oligos for Illumina were purchased from New England Biolabs (USA). .. Deoxynucleotide triphosphates (dNTPs), UNIQ-10 Spin Column Oligo DNA Purification Kits, EZ-10 Column DNA Purification Kits and 1 × phosphate buffer saline (PBS, pH 7.4) were ordered from Shanghai Sangon Biological Engineering Technology Services Co., Ltd. (Shanghai, China).

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  • 87
    New England Biolabs nebnext adaptors
    Nebnext Adaptors, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext adaptors/product/New England Biolabs
    Average 87 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    nebnext adaptors - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

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