cutseq validation  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    NEBNext Singleplex Oligos for Illumina
    Description:
    NEBNext Singleplex Oligos for Illumina 60 rxns
    Catalog Number:
    e7350l
    Price:
    243
    Size:
    60 rxns
    Category:
    DNA Analysis
    Buy from Supplier


    Structured Review

    New England Biolabs cutseq validation
    NEBNext Singleplex Oligos for Illumina
    NEBNext Singleplex Oligos for Illumina 60 rxns
    https://www.bioz.com/result/cutseq validation/product/New England Biolabs
    Average 93 stars, based on 2176 article reviews
    Price from $9.99 to $1999.99
    cutseq validation - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples"

    Article Title: CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

    Journal: Nature Communications

    doi: 10.1038/s41467-019-12570-2

    CUTseq validation. a Copy number profiles (NlaIII, 100 kb resolution) determined with CUTseq and NEBNext using gDNA extracted from ten different FPPE tumors. BRCA, breast cancer. COAD, colon adenocarcinoma. GIST, gastrointestinal stromal tumor. MELA, melanoma. ρ , Pearson’s correlation between matched profiles. b Pearson’s correlation ( ρ ) between the fraction of the genome (100 kb resolution) either amplified or deleted in each of the paired CUTseq and NEBNext samples shown in a . Each dot represents one pair of replicates. Dashed line: linear regression. c Partitioning of all the SNVs called in two replicate (Rep) exome capture experiments using SKBR3 cells gDNA and either CUTseq or a commercial kit for library preparation (Agilent), in multiple different annotated genomic regions. Up- and downstream indicate 5 kb windows before the start codons and after the stop codons of protein-coding genes, respectively. d Same as in c , but for different substitution types. e Overlap between the high-confidence SNVs (at least 50× coverage) called in the two CUTseq replicates shown in c , d . f Overlap between all the high-confidence SNVs identified by CUTseq vs. Agilent, after merging matched replicates shown in c , d . In both e and f , the percentages refer to the total number of SNVs in the union of the two sets. All the source data for this figure are provided as a Source Data file
    Figure Legend Snippet: CUTseq validation. a Copy number profiles (NlaIII, 100 kb resolution) determined with CUTseq and NEBNext using gDNA extracted from ten different FPPE tumors. BRCA, breast cancer. COAD, colon adenocarcinoma. GIST, gastrointestinal stromal tumor. MELA, melanoma. ρ , Pearson’s correlation between matched profiles. b Pearson’s correlation ( ρ ) between the fraction of the genome (100 kb resolution) either amplified or deleted in each of the paired CUTseq and NEBNext samples shown in a . Each dot represents one pair of replicates. Dashed line: linear regression. c Partitioning of all the SNVs called in two replicate (Rep) exome capture experiments using SKBR3 cells gDNA and either CUTseq or a commercial kit for library preparation (Agilent), in multiple different annotated genomic regions. Up- and downstream indicate 5 kb windows before the start codons and after the stop codons of protein-coding genes, respectively. d Same as in c , but for different substitution types. e Overlap between the high-confidence SNVs (at least 50× coverage) called in the two CUTseq replicates shown in c , d . f Overlap between all the high-confidence SNVs identified by CUTseq vs. Agilent, after merging matched replicates shown in c , d . In both e and f , the percentages refer to the total number of SNVs in the union of the two sets. All the source data for this figure are provided as a Source Data file

    Techniques Used: Amplification

    Related Articles

    Multiplex Assay:

    Article Title: RNA sequencing analysis of salivary extracellular RNA
    Article Snippet: .. *The Universal PCR primer and Index (X) Primer are contained in the NEBNext SinglePlex (NEB #E7350) or NEBNext Multiplex (NEB #E7335 or NEB #E7500) Oligos for Illumina. ..

    Article Title: RNA sequencing analysis of salivary extracellular RNA
    Article Snippet: .. *The adaptor is provided in NEBBext Singleplex (NEB #E7350) or NEBNext Multiplex (NEB #E7335, #E7500) Oligos for Illumina ..

    RNA Sequencing Assay:

    Article Title: Cell Type-Specific Expression Profile and Signaling Requirements in Early Hematopoietic Reprogramming
    Article Snippet: .. An RNA-seq library was prepared using the protocol of NEBNext Singleplex (#E7350) oligos from Illumina. ..

    Synthesized:

    Article Title: Folding heterogeneity in the essential human telomerase RNA three-way junction
    Article Snippet: .. The DNA templates were assembled from DNA oligonucleotides designed using the Primerize tool ( ) and synthesized by IDT (Fig. S7 and Table S2). .. In the event that a complete DNA template could not be synthesized by one primer assembly reaction using Phusion polymerase (NEB), a ‘two-piece’ scheme was employed, in which the products of two separate primer assemblies were used to generate the complete DNA product.

    Purification:

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Real-time Polymerase Chain Reaction:

    Article Title: Tagger—A Swiss army knife for multiomics to dissect cell type–specific mechanisms of gene expression in mice
    Article Snippet: .. In brief, samples were mixed with index (#E7350, NEB, Ipswich, MA), universal primers, SyBr dye, and 2X NEBNext Ultra II Q5 master mix containing high fidelity DNA Polymerase (cat. M0544S, NEB, Ipswich, MA), and analyzed by qPCR. .. Methylated DNA IPed samples were further amplified to prepare libraries for sequencing.

    Polymerase Chain Reaction:

    Article Title: RNA sequencing analysis of salivary extracellular RNA
    Article Snippet: .. *The Universal PCR primer and Index (X) Primer are contained in the NEBNext SinglePlex (NEB #E7350) or NEBNext Multiplex (NEB #E7335 or NEB #E7500) Oligos for Illumina. ..

    other:

    Article Title: High-throughput in vitro specificity profiling of natural and high-fidelity CRISPR-Cas9 variants
    Article Snippet: Supplementary Table 1 has the sequences of DNA oligonucleotides and RNA used in this study.

    Article Title: A second hybrid-binding domain modulates the activity of Drosophila ribonuclease H1
    Article Snippet: Nucleic acid substrates RNA and DNA oligonucleotides used for the experiments are listed in Table S1.

    cDNA Library Assay:

    Article Title: Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil
    Article Snippet: .. A cDNA library was prepared from viral RNA by using a NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Singleplex Oligos for Illumina (New England Biolabs), followed by purification by using Agencourt AMPure XP (Beckman Coulter). .. The library was sequenced by using MiSeq Reagent Kits v2 with MiSeq (Illumina).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs nebnext singleplex oligos for illumina
    Nebnext Singleplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext singleplex oligos for illumina/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nebnext singleplex oligos for illumina - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results