luna universal qpcr master mix  (New England Biolabs)


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    Luna Universal qPCR Master Mix
    Description:
    Luna Universal qPCR Master Mix 2 500 rxns
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    m3003e
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    2 500 rxns
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    New England Biolabs luna universal qpcr master mix
    Luna Universal qPCR Master Mix
    Luna Universal qPCR Master Mix 2 500 rxns
    https://www.bioz.com/result/luna universal qpcr master mix/product/New England Biolabs
    Average 99 stars, based on 3722 article reviews
    Price from $9.99 to $1999.99
    luna universal qpcr master mix - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like Proteins"

    Article Title: Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like Proteins

    Journal: mSphere

    doi: 10.1128/mSphere.00806-19

    Expression analysis of Mga regulon genes. cDNA from 19 isolates grown to mid-log phase in rich medium were analyzed for the expression of Mga regulon genes. The isolates were selected to be representative of all possible Mga regulon configurations and emm cluster diversity where possible. Primers were designed to amplify all members of the gene family where possible ( mrp , enn , pgs , sph , and scpA ) and to amplify a subset where sequence diversity necessitates. The dot plot symbols represent the mean value of the four qPCR analyses for each isolate, and the error bars represent the standard errors for all isolates for each gene.
    Figure Legend Snippet: Expression analysis of Mga regulon genes. cDNA from 19 isolates grown to mid-log phase in rich medium were analyzed for the expression of Mga regulon genes. The isolates were selected to be representative of all possible Mga regulon configurations and emm cluster diversity where possible. Primers were designed to amplify all members of the gene family where possible ( mrp , enn , pgs , sph , and scpA ) and to amplify a subset where sequence diversity necessitates. The dot plot symbols represent the mean value of the four qPCR analyses for each isolate, and the error bars represent the standard errors for all isolates for each gene.

    Techniques Used: Expressing, Sequencing, Real-time Polymerase Chain Reaction

    2) Product Images from "Interplay of BAF and MLL4 promotes cell type-specific enhancer activation"

    Article Title: Interplay of BAF and MLL4 promotes cell type-specific enhancer activation

    Journal: bioRxiv

    doi: 10.1101/2020.06.24.168146

    PBAF-specific subunit PBRM1 is dispensable for adipogenesis in vivo and in culture ( A-E ) Characterization of adult (8 week-old) Pbrm1 f/f ; Myf5-Cre (cKO) mice. Pbrm1 f/f (f/f) were crossed with Pbrm1 f/+ ; Myf5-Cre to obtain cKO mice. (A) Genotyping results. The expected ratios of the four genotypes are 1:1:1:1. (B) Representative pictures of 8-week-old f/f or cKO mice. (C) The average adipose tissue weights in f/f ( n = 5) and cKO ( n = 5) mice are shown as % of body weight. n.s., not significant. (D) Pictures of interscapular BAT isolated from three f/f or cKO mice. (E) Total RNA was extracted from BAT of f/f ( n = 5) or cKO ( n = 5) mice for qRT-PCR analysis of Pbrm1 , adipogenesis markers Pparg, Cebpα and Fabp4 and BAT marker Ucp1 . Quantitative PCR data in all figures are presented as means ± SEM. *** p
    Figure Legend Snippet: PBAF-specific subunit PBRM1 is dispensable for adipogenesis in vivo and in culture ( A-E ) Characterization of adult (8 week-old) Pbrm1 f/f ; Myf5-Cre (cKO) mice. Pbrm1 f/f (f/f) were crossed with Pbrm1 f/+ ; Myf5-Cre to obtain cKO mice. (A) Genotyping results. The expected ratios of the four genotypes are 1:1:1:1. (B) Representative pictures of 8-week-old f/f or cKO mice. (C) The average adipose tissue weights in f/f ( n = 5) and cKO ( n = 5) mice are shown as % of body weight. n.s., not significant. (D) Pictures of interscapular BAT isolated from three f/f or cKO mice. (E) Total RNA was extracted from BAT of f/f ( n = 5) or cKO ( n = 5) mice for qRT-PCR analysis of Pbrm1 , adipogenesis markers Pparg, Cebpα and Fabp4 and BAT marker Ucp1 . Quantitative PCR data in all figures are presented as means ± SEM. *** p

    Techniques Used: In Vivo, Mouse Assay, Isolation, Quantitative RT-PCR, Marker, Real-time Polymerase Chain Reaction

    SMARCB1 is required for adipogenesis in vivo and in culture ( A-D ) Smarcb1 f/f ; PdgfRα-Cre (cKO) mice showed defects in skull bone and died shortly after birth. (A) Genotype of progeny from the crossing between Smarcb1 f/f (f/f) and Smarcb1 f/+ ; PdgfRα-Cre mice at embryonic day 18.5 (E18.5), post-natal day 0.5 (P0.5) and weaning (3 weeks of age). Dead pups are indicated by an asterisk. (B) Representative morphology of E18.5 embryos. (C) Defective coronal sutures in cKO mice. (D) H E staining of the sagittal sections of head. Arrows indicate the skull bone. ( E-F ) Impaired BAT development in cKO mice. (E) Representative pictures of interscapular BAT. (F) qRT-PCR analysis of Smarcb1 , adipocyte-enriched genes Pparg, Cebpa, Fabp4, Prdm16 and Ucp1 as well as myogenesis marker genes Myogenin, MyHC and MCK in BAT isolated from E18.5 f/f ( n = 5) and cKO ( n = 5) embryos. Quantitative PCR data is presented as means ± SEM. *** p
    Figure Legend Snippet: SMARCB1 is required for adipogenesis in vivo and in culture ( A-D ) Smarcb1 f/f ; PdgfRα-Cre (cKO) mice showed defects in skull bone and died shortly after birth. (A) Genotype of progeny from the crossing between Smarcb1 f/f (f/f) and Smarcb1 f/+ ; PdgfRα-Cre mice at embryonic day 18.5 (E18.5), post-natal day 0.5 (P0.5) and weaning (3 weeks of age). Dead pups are indicated by an asterisk. (B) Representative morphology of E18.5 embryos. (C) Defective coronal sutures in cKO mice. (D) H E staining of the sagittal sections of head. Arrows indicate the skull bone. ( E-F ) Impaired BAT development in cKO mice. (E) Representative pictures of interscapular BAT. (F) qRT-PCR analysis of Smarcb1 , adipocyte-enriched genes Pparg, Cebpa, Fabp4, Prdm16 and Ucp1 as well as myogenesis marker genes Myogenin, MyHC and MCK in BAT isolated from E18.5 f/f ( n = 5) and cKO ( n = 5) embryos. Quantitative PCR data is presented as means ± SEM. *** p

    Techniques Used: In Vivo, Mouse Assay, Staining, Quantitative RT-PCR, Marker, Isolation, Real-time Polymerase Chain Reaction

    3) Product Images from "Interplay of BAF and MLL4 promotes cell type-specific enhancer activation"

    Article Title: Interplay of BAF and MLL4 promotes cell type-specific enhancer activation

    Journal: bioRxiv

    doi: 10.1101/2020.06.24.168146

    BAF subunits SMARCA4, SMARCB1 and ARID1A are required for adipogenesis ( A-C ) Depletion of SMARCA4 by auxin-inducible degron (AID) system inhibits adipogenesis. (A) A schematic illustration of the auxin-induced depletion of endogenous SMARCA4 protein in Tir1-expressing Smarca4 AID/AID cells. TIR1, transport inhibitor response 1; Cul1, Cullin-1; Rbx1, RING-box protein 1; Skp1, S-phase kinase-associated protein 1. (B) Western blot analyses using antibodies indicated on the left. RbBP5 was used as a loading control. (C) Oil Red O staining at day 7 (D7) of adipogenesis. Cells were treated with auxin throughout the differentiation. (D) SMARCB1 is essential for adipogenesis in vivo . Sections of the interscapular area of E18.5 Smarcb1 f/f (f/f) and Smarcb1 f/f ; PdgfRα-Cre (cKO) embryos were stained with H E (left panels) or with antibodies recognizing brown adipose tissue (BAT) marker Ucp1 (green) and skeletal muscle marker Myosin (red) (right panels). B, BAT; M, muscle. Scale bar = 1mm. ( E-H ) SMARCB1 is essential for adipogenesis in culture. SV40T-immortalized Smarcb1 f/f preadipocytes were infected with adenoviral GFP or Cre, followed by adipogenesis assays. (E) Deletion of Smarcb1 does not affect cell growth rates. 1 x 10 5 preadipocytes were plated and the cumulative cell numbers were determined for 5 days. (F) Before (D-3) and during (D2) adipogenesis, nuclear extracts were analyzed by Western blot using indicated antibodies. (G) Oil Red O staining at D7 of adipogenesis. (H) Expression of Cebpb, Pparg, Cebpa and Fabp4 before (D-3) and during (D2) adipogenesis was determined using RNA-Seq. RPKM values indicate gene expression levels. ( I - K ) ARID1A is required for adipogenesis in culture. Preadipocytes were infected with lentiviral vectors expressing control (shCtrl) or Arid1a knockdown shRNA (shArid1a), followed by adipogenesis assays. (I) Western blot analysis of ARID1A in preadipocytes. (J) Oil Red O staining at D7 of adipogenesis. (K) qRT-PCR analysis of adipogenesis marker expression at indicated time points of adipogenesis.
    Figure Legend Snippet: BAF subunits SMARCA4, SMARCB1 and ARID1A are required for adipogenesis ( A-C ) Depletion of SMARCA4 by auxin-inducible degron (AID) system inhibits adipogenesis. (A) A schematic illustration of the auxin-induced depletion of endogenous SMARCA4 protein in Tir1-expressing Smarca4 AID/AID cells. TIR1, transport inhibitor response 1; Cul1, Cullin-1; Rbx1, RING-box protein 1; Skp1, S-phase kinase-associated protein 1. (B) Western blot analyses using antibodies indicated on the left. RbBP5 was used as a loading control. (C) Oil Red O staining at day 7 (D7) of adipogenesis. Cells were treated with auxin throughout the differentiation. (D) SMARCB1 is essential for adipogenesis in vivo . Sections of the interscapular area of E18.5 Smarcb1 f/f (f/f) and Smarcb1 f/f ; PdgfRα-Cre (cKO) embryos were stained with H E (left panels) or with antibodies recognizing brown adipose tissue (BAT) marker Ucp1 (green) and skeletal muscle marker Myosin (red) (right panels). B, BAT; M, muscle. Scale bar = 1mm. ( E-H ) SMARCB1 is essential for adipogenesis in culture. SV40T-immortalized Smarcb1 f/f preadipocytes were infected with adenoviral GFP or Cre, followed by adipogenesis assays. (E) Deletion of Smarcb1 does not affect cell growth rates. 1 x 10 5 preadipocytes were plated and the cumulative cell numbers were determined for 5 days. (F) Before (D-3) and during (D2) adipogenesis, nuclear extracts were analyzed by Western blot using indicated antibodies. (G) Oil Red O staining at D7 of adipogenesis. (H) Expression of Cebpb, Pparg, Cebpa and Fabp4 before (D-3) and during (D2) adipogenesis was determined using RNA-Seq. RPKM values indicate gene expression levels. ( I - K ) ARID1A is required for adipogenesis in culture. Preadipocytes were infected with lentiviral vectors expressing control (shCtrl) or Arid1a knockdown shRNA (shArid1a), followed by adipogenesis assays. (I) Western blot analysis of ARID1A in preadipocytes. (J) Oil Red O staining at D7 of adipogenesis. (K) qRT-PCR analysis of adipogenesis marker expression at indicated time points of adipogenesis.

    Techniques Used: Expressing, Western Blot, Staining, In Vivo, Marker, Infection, RNA Sequencing Assay, shRNA, Quantitative RT-PCR

    PBAF-specific subunit PBRM1 is dispensable for adipogenesis in vivo and in culture ( A-E ) Characterization of adult (8 week-old) Pbrm1 f/f ; Myf5-Cre (cKO) mice. Pbrm1 f/f (f/f) were crossed with Pbrm1 f/+ ; Myf5-Cre to obtain cKO mice. (A) Genotyping results. The expected ratios of the four genotypes are 1:1:1:1. (B) Representative pictures of 8-week-old f/f or cKO mice. (C) The average adipose tissue weights in f/f ( n = 5) and cKO ( n = 5) mice are shown as % of body weight. n.s., not significant. (D) Pictures of interscapular BAT isolated from three f/f or cKO mice. (E) Total RNA was extracted from BAT of f/f ( n = 5) or cKO ( n = 5) mice for qRT-PCR analysis of Pbrm1 , adipogenesis markers Pparg, Cebpα and Fabp4 and BAT marker Ucp1 . Quantitative PCR data in all figures are presented as means ± SEM. *** p
    Figure Legend Snippet: PBAF-specific subunit PBRM1 is dispensable for adipogenesis in vivo and in culture ( A-E ) Characterization of adult (8 week-old) Pbrm1 f/f ; Myf5-Cre (cKO) mice. Pbrm1 f/f (f/f) were crossed with Pbrm1 f/+ ; Myf5-Cre to obtain cKO mice. (A) Genotyping results. The expected ratios of the four genotypes are 1:1:1:1. (B) Representative pictures of 8-week-old f/f or cKO mice. (C) The average adipose tissue weights in f/f ( n = 5) and cKO ( n = 5) mice are shown as % of body weight. n.s., not significant. (D) Pictures of interscapular BAT isolated from three f/f or cKO mice. (E) Total RNA was extracted from BAT of f/f ( n = 5) or cKO ( n = 5) mice for qRT-PCR analysis of Pbrm1 , adipogenesis markers Pparg, Cebpα and Fabp4 and BAT marker Ucp1 . Quantitative PCR data in all figures are presented as means ± SEM. *** p

    Techniques Used: In Vivo, Mouse Assay, Isolation, Quantitative RT-PCR, Marker, Real-time Polymerase Chain Reaction

    SMARCB1 is required for adipogenesis in vivo and in culture ( A-D ) Smarcb1 f/f ; PdgfRα-Cre (cKO) mice showed defects in skull bone and died shortly after birth. (A) Genotype of progeny from the crossing between Smarcb1 f/f (f/f) and Smarcb1 f/+ ; PdgfRα-Cre mice at embryonic day 18.5 (E18.5), post-natal day 0.5 (P0.5) and weaning (3 weeks of age). Dead pups are indicated by an asterisk. (B) Representative morphology of E18.5 embryos. (C) Defective coronal sutures in cKO mice. (D) H E staining of the sagittal sections of head. Arrows indicate the skull bone. ( E-F ) Impaired BAT development in cKO mice. (E) Representative pictures of interscapular BAT. (F) qRT-PCR analysis of Smarcb1 , adipocyte-enriched genes Pparg, Cebpa, Fabp4, Prdm16 and Ucp1 as well as myogenesis marker genes Myogenin, MyHC and MCK in BAT isolated from E18.5 f/f ( n = 5) and cKO ( n = 5) embryos. Quantitative PCR data is presented as means ± SEM. *** p
    Figure Legend Snippet: SMARCB1 is required for adipogenesis in vivo and in culture ( A-D ) Smarcb1 f/f ; PdgfRα-Cre (cKO) mice showed defects in skull bone and died shortly after birth. (A) Genotype of progeny from the crossing between Smarcb1 f/f (f/f) and Smarcb1 f/+ ; PdgfRα-Cre mice at embryonic day 18.5 (E18.5), post-natal day 0.5 (P0.5) and weaning (3 weeks of age). Dead pups are indicated by an asterisk. (B) Representative morphology of E18.5 embryos. (C) Defective coronal sutures in cKO mice. (D) H E staining of the sagittal sections of head. Arrows indicate the skull bone. ( E-F ) Impaired BAT development in cKO mice. (E) Representative pictures of interscapular BAT. (F) qRT-PCR analysis of Smarcb1 , adipocyte-enriched genes Pparg, Cebpa, Fabp4, Prdm16 and Ucp1 as well as myogenesis marker genes Myogenin, MyHC and MCK in BAT isolated from E18.5 f/f ( n = 5) and cKO ( n = 5) embryos. Quantitative PCR data is presented as means ± SEM. *** p

    Techniques Used: In Vivo, Mouse Assay, Staining, Quantitative RT-PCR, Marker, Isolation, Real-time Polymerase Chain Reaction

    Related Articles

    Transduction:

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    Article Snippet: .. Quantification of gDNA/ integrated viral DNA ratio At 48 h post transduction, integrated lentiviral DNA was quantified by extracting genomic DNA using Qiamp DNA Mini kit (Qiagen, Germany) and the ratio of viral genome: human gDNA were estimated using qPCR via Luna Universal qPCR Master Mix (New England Biolabs) using designed primers Gag-Fwd: GGA GCT AGA ACG ATT CGC AGT TA, Gag-Rev: GGT TGT AGC TGT CCC AGT ATT TG TC, PBS-Fwd: TCT CGA CGC AGG ACT CG; PBS-Rev: TAC TGA CGC TCT CGC ACC, and β-actin forward and reverse primers described above. ..

    Real-time Polymerase Chain Reaction:

    Article Title: CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
    Article Snippet: .. DNA from symptomatic field plant of Agbagba plant and asymptomatic in vitro plantlet of Cavendish Williams were used as positive and negative controls, respectively. qPCR was performed in a 20 µL reaction volume containing 60 ng of genomic DNA, 10 µL of Luna Universal qPCR master mix (New England Biolab), and 0.3 µL of 10 µM of each primer (P4F and P4R, Supplementary Table ). qPCR was performed using the ABI 7500 real-time machine (Applied Biosystem, USA). .. The means and standard deviation were calculated for CT values of six technical replicates from two independent experiments, using Minitab 14 statistical software, 2012.

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    Article Title: Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like Proteins
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    Article Title: β1 integrin, ILK and mTOR regulate collagen synthesis in mechanically loaded tendon cells
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    Article Title: Deformed Wing Virus spillover from honey bees to bumble bees: a reverse genetic study
    Article Snippet: .. The quantification of DWV genome copies was performed by SYBR-Green Real-Time Quantitative PCR (qPCR) using Luna Universal qPCR master mix (New England Biolabs), 0.25 μM forward and reverse DWV_qPCR primers and 2 μl of cDNA. ..

    Article Title: Promoter Choice: Who Should Drive the CAR in T Cells?
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    Article Title: TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism
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    Article Title: Novel mosaic mice with diverse applications
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    Amplification:

    Article Title: Nutrient Sensing, Nutrition, and Metabolism: Hepatic ZIP8 deficiency is associated with disrupted selenium homeostasis, liver pathology, and tumor formation
    Article Snippet: .. The quantitative (q)PCR for individual gene expression was carried out with Luna Universal qPCR Master Mix (New England Biolabs) and gene-specific primers; cycling conditions included 95°C for 1 min, followed by amplification for 40 cycles at 95°C for 15 s, and then 60°C for 30 s in the CFX96 Real-Time PCR system (Bio-Rad). .. Relative expression of mRNA levels was normalized to that of the housekeeping control gene Gapdh , using the ΔΔCq method.

    Article Title: Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like Proteins
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    In Vitro:

    Article Title: CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
    Article Snippet: .. DNA from symptomatic field plant of Agbagba plant and asymptomatic in vitro plantlet of Cavendish Williams were used as positive and negative controls, respectively. qPCR was performed in a 20 µL reaction volume containing 60 ng of genomic DNA, 10 µL of Luna Universal qPCR master mix (New England Biolab), and 0.3 µL of 10 µM of each primer (P4F and P4R, Supplementary Table ). qPCR was performed using the ABI 7500 real-time machine (Applied Biosystem, USA). .. The means and standard deviation were calculated for CT values of six technical replicates from two independent experiments, using Minitab 14 statistical software, 2012.

    Synthesized:

    Article Title: β1 integrin, ILK and mTOR regulate collagen synthesis in mechanically loaded tendon cells
    Article Snippet: .. The primers (Table ) were designed by PrimerQuest Tool and synthesized by Integrated DNA Technologies. qPCR was run using 7500 Fast Real-Time PCR System (Applied Biosystems, USA) and Luna Universal qPCR Master Mix (New England Biolabs, USA, #M3003) according to the manufacturer’s instructions. .. Each cDNA samples were run as duplicates using 10 ng cDNA for each qPCR reaction.

    SYBR Green Assay:

    Article Title: Deformed Wing Virus spillover from honey bees to bumble bees: a reverse genetic study
    Article Snippet: .. The quantification of DWV genome copies was performed by SYBR-Green Real-Time Quantitative PCR (qPCR) using Luna Universal qPCR master mix (New England Biolabs), 0.25 μM forward and reverse DWV_qPCR primers and 2 μl of cDNA. ..

    Expressing:

    Article Title: Nutrient Sensing, Nutrition, and Metabolism: Hepatic ZIP8 deficiency is associated with disrupted selenium homeostasis, liver pathology, and tumor formation
    Article Snippet: .. The quantitative (q)PCR for individual gene expression was carried out with Luna Universal qPCR Master Mix (New England Biolabs) and gene-specific primers; cycling conditions included 95°C for 1 min, followed by amplification for 40 cycles at 95°C for 15 s, and then 60°C for 30 s in the CFX96 Real-Time PCR system (Bio-Rad). .. Relative expression of mRNA levels was normalized to that of the housekeeping control gene Gapdh , using the ΔΔCq method.

    Article Title: Novel mosaic mice with diverse applications
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    Polymerase Chain Reaction:

    Article Title: TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism
    Article Snippet: .. Quantitative realtime PCR was performed with a CFX Connect Real-Time PCR Detection system (Bio-Rad Laboratories) and the Luna Universal qPCR Master Mix (New England Biolabs) in technical triplicates with 20 µl reaction volume and 2 µl of a 1:10 dilution of the cDNA template. .. Gapdh was used as reference gene for mouse qPCR and eef1a1l was used for zebrafish qPCR.

    Countercurrent Chromatography:

    Article Title: Promoter Choice: Who Should Drive the CAR in T Cells?
    Article Snippet: .. Quantification of gDNA/ integrated viral DNA ratio At 48 h post transduction, integrated lentiviral DNA was quantified by extracting genomic DNA using Qiamp DNA Mini kit (Qiagen, Germany) and the ratio of viral genome: human gDNA were estimated using qPCR via Luna Universal qPCR Master Mix (New England Biolabs) using designed primers Gag-Fwd: GGA GCT AGA ACG ATT CGC AGT TA, Gag-Rev: GGT TGT AGC TGT CCC AGT ATT TG TC, PBS-Fwd: TCT CGA CGC AGG ACT CG; PBS-Rev: TAC TGA CGC TCT CGC ACC, and β-actin forward and reverse primers described above. ..

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    New England Biolabs protoscript ii first strand cdna enzyme
    Protoscript Ii First Strand Cdna Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoscript ii first strand cdna enzyme/product/New England Biolabs
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
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