dna fragment library preparation kit  (New England Biolabs)


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    Name:
    NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads
    Description:
    NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads 96 rxns
    Catalog Number:
    e6177l
    Price:
    2625
    Size:
    96 rxns
    Category:
    DNA Template Preparation for PCR
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    New England Biolabs dna fragment library preparation kit
    NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads
    NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads 96 rxns
    https://www.bioz.com/result/dna fragment library preparation kit/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragment library preparation kit - by Bioz Stars, 2020-02
    91/100 stars

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    Flow Cytometry:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions. .. The libraries were then enriched by 10-20 PCR cycles using a transposon-specific forward primer and a reverse primer that included Illumina flow cell binding sites as described previously .

    Mutagenesis:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: Genomic DNA (gDNA) of the initial (input) and selected (output) mutant pools was extracted using Qiagen Genomic-tip 100/G (Qiagen, Hiden, Germany, catalogue number 10043). .. The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions.

    Purification:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions. .. The enriched libraries were purified using the Agen court AMPure XP beads (Beckman Coulter, catalogue number A63882) and quantified on an Agilent DNA1000 chip (Agilent Technologies, Catalogue number 5067-1504) as per the manufacturer’s instructions.

    Sequencing:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: Transposon directed insertion site sequencing (TraDIS) . .. The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: After 180 minutes, the entire serum-bacteria suspension was transferred to 25 ml LB broth, vortexed and incubated overnight aerobically 37°C, to enable the surviving library to outgrow killed components. .. The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions. .. The libraries were then enriched by 10-20 PCR cycles using a transposon-specific forward primer and a reverse primer that included Illumina flow cell binding sites as described previously .

    Binding Assay:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions. .. The libraries were then enriched by 10-20 PCR cycles using a transposon-specific forward primer and a reverse primer that included Illumina flow cell binding sites as described previously .

    Chromatin Immunoprecipitation:

    Article Title: Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasiveSalmonella Typhimurium ST313
    Article Snippet: The fragmented DNA was end repaired, A-tailed and adapter-ligated using Illumina DNA fragment library preparation kit (New England BioLabs Inc., catalogue number E6177) according to the manufacturer’s instructions. .. The enriched libraries were purified using the Agen court AMPure XP beads (Beckman Coulter, catalogue number A63882) and quantified on an Agilent DNA1000 chip (Agilent Technologies, Catalogue number 5067-1504) as per the manufacturer’s instructions.

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  • 91
    New England Biolabs dna fragment library preparation kit
    Dna Fragment Library Preparation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragment library preparation kit/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragment library preparation kit - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

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