small rna sample preparation kit  (New England Biolabs)


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    Structured Review

    New England Biolabs small rna sample preparation kit
    Small Rna Sample Preparation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna sample preparation kit/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    small rna sample preparation kit - by Bioz Stars, 2019-07
    79/100 stars

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    Related Articles

    Modification:

    Article Title: Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection
    Article Snippet: About 100 ng mRNA was fragmented using NEB Next Magnesium RNA Fragmentation Module (E6150, NEB), purified with an RNA clean up kit (R6247, Omega, USA), end repaired with T4 Polynucleotide Kinase (T4 PNK) (M0201, NEB) and cleaned up again with a kit (R6247, Omega). .. The end-repaired RNAs were used to prepare the strand specific transcriptome, using the Small RNA Sample Preparation kit (E6160, NEB) according to the manufacturer protocol with some minor modification, including the SR Primer F3 being replaced with barcode primers. .. The resulting DNA and RNA libraries were used for emulsion PCR to produce the beads for sequencing on the SOLiD 5500 machine, using 75 nt mode and 75 nt + 35 nt mode for the sequencing of DNA and RNA libraries, respectively.

    Polymerase Chain Reaction:

    Article Title: Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection
    Article Snippet: Paragraph title: DNA and RNA library preparation, emulsion PCR and sequencing ... The end-repaired RNAs were used to prepare the strand specific transcriptome, using the Small RNA Sample Preparation kit (E6160, NEB) according to the manufacturer protocol with some minor modification, including the SR Primer F3 being replaced with barcode primers.

    Sequencing:

    Article Title: Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection
    Article Snippet: Paragraph title: DNA and RNA library preparation, emulsion PCR and sequencing ... The end-repaired RNAs were used to prepare the strand specific transcriptome, using the Small RNA Sample Preparation kit (E6160, NEB) according to the manufacturer protocol with some minor modification, including the SR Primer F3 being replaced with barcode primers.

    Sample Prep:

    Article Title: Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection
    Article Snippet: About 100 ng mRNA was fragmented using NEB Next Magnesium RNA Fragmentation Module (E6150, NEB), purified with an RNA clean up kit (R6247, Omega, USA), end repaired with T4 Polynucleotide Kinase (T4 PNK) (M0201, NEB) and cleaned up again with a kit (R6247, Omega). .. The end-repaired RNAs were used to prepare the strand specific transcriptome, using the Small RNA Sample Preparation kit (E6160, NEB) according to the manufacturer protocol with some minor modification, including the SR Primer F3 being replaced with barcode primers. .. The resulting DNA and RNA libraries were used for emulsion PCR to produce the beads for sequencing on the SOLiD 5500 machine, using 75 nt mode and 75 nt + 35 nt mode for the sequencing of DNA and RNA libraries, respectively.

    Purification:

    Article Title: Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection
    Article Snippet: About 100 ng mRNA was fragmented using NEB Next Magnesium RNA Fragmentation Module (E6150, NEB), purified with an RNA clean up kit (R6247, Omega, USA), end repaired with T4 Polynucleotide Kinase (T4 PNK) (M0201, NEB) and cleaned up again with a kit (R6247, Omega). .. The end-repaired RNAs were used to prepare the strand specific transcriptome, using the Small RNA Sample Preparation kit (E6160, NEB) according to the manufacturer protocol with some minor modification, including the SR Primer F3 being replaced with barcode primers.

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  • 79
    New England Biolabs small rna sample preparation kit
    Small Rna Sample Preparation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna sample preparation kit/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    small rna sample preparation kit - by Bioz Stars, 2019-07
    79/100 stars
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