nextquick 454 library prep kit e6090  (New England Biolabs)


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    Structured Review

    New England Biolabs nextquick 454 library prep kit e6090
    Nextquick 454 Library Prep Kit E6090, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextquick 454 library prep kit e6090/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nextquick 454 library prep kit e6090 - by Bioz Stars, 2019-08
    80/100 stars

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    Amplification:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: Briefly, the PCR amplicons were purified using AMPure beads (Agencourt Bioscience Corporation, Beverly, MA, USA), adaptors were blunt-end ligated to the fragment and the dsDNA amplicon library was quantified via fluorometry using Quanti-iT Pico Green reagents (Invitrogen, Carlsbad, CA, USA). .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK). .. Fosmids were extracted using FosmidMAX DNA purification kit (Epicentre, Madison, WI, USA) and sequenced using an Illumina MISEQ sequencing system at the Centre for Genomic Research, Liverpool.

    Construct:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: The cells were centrifuge-concentrated and used to construct a metagenomic library for 454 pyrosequencing. .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK).

    Purification:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: Briefly, the PCR amplicons were purified using AMPure beads (Agencourt Bioscience Corporation, Beverly, MA, USA), adaptors were blunt-end ligated to the fragment and the dsDNA amplicon library was quantified via fluorometry using Quanti-iT Pico Green reagents (Invitrogen, Carlsbad, CA, USA). .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK).

    Generated:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: An amplicon library was generated using the Rapid Library Preparation kit and following the manufacturer’s recommendations in the GS FLX Titanium Series Rapid Library Preparation Method Manual (Roche, Branford. .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK).

    Polymerase Chain Reaction:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: Briefly, the PCR amplicons were purified using AMPure beads (Agencourt Bioscience Corporation, Beverly, MA, USA), adaptors were blunt-end ligated to the fragment and the dsDNA amplicon library was quantified via fluorometry using Quanti-iT Pico Green reagents (Invitrogen, Carlsbad, CA, USA). .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK). .. Fosmids were extracted using FosmidMAX DNA purification kit (Epicentre, Madison, WI, USA) and sequenced using an Illumina MISEQ sequencing system at the Centre for Genomic Research, Liverpool.

    Plasmid Preparation:

    Article Title: Extremophiles in an Antarctic Marine Ecosystem
    Article Snippet: QIAGEN, Manchester, UK) and then treated with ATP-dependent exo-nuclease (Plasmid-SafeTM ATP-Dependent DNase, 10 U·µL−1 10,000 U, Cat. No. E3110K, Epicentre, Madison, WI, USA). .. The library was then subjected to clonal amplification by emulsion PCR followed by pyrosequencing on a 454 GS FLX sequencer according to the manufacturer’s instructions (NEB NextQuick 454 library prep kit E6090, Hitchin, Herts, UK).

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    New England Biolabs nextquick 454 library prep kit e6090
    Nextquick 454 Library Prep Kit E6090, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextquick 454 library prep kit e6090/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nextquick 454 library prep kit e6090 - by Bioz Stars, 2019-08
    80/100 stars
      Buy from Supplier

    99
    New England Biolabs dna library prep master mix set
    Dna Library Prep Master Mix Set, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna library prep master mix set/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dna library prep master mix set - by Bioz Stars, 2019-08
    99/100 stars
      Buy from Supplier

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