e6070  (New England Biolabs)


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    Name:
    NEBNext DNA Library Prep Master Mix Set for 454
    Description:
    NEBNext DNA Library Prep Master Mix Set for 454 50 rxns
    Catalog Number:
    e6070l
    Price:
    1260
    Size:
    50 rxns
    Category:
    DNA Analysis
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    Structured Review

    New England Biolabs e6070
    NEBNext DNA Library Prep Master Mix Set for 454
    NEBNext DNA Library Prep Master Mix Set for 454 50 rxns
    https://www.bioz.com/result/e6070/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e6070 - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Selection:

    Article Title: High temporal and spatial diversity in marine RNA viruses implies that they have an important role in mortality and structuring plankton communities
    Article Snippet: .. Library prep and pyrosequencing Libraries for each site were prepared for sequencing using NEBNext DNA Library Prep for 454 kit (New England Biolabs, Ipswich, Massachusetts, USA) following the manufacturer's directions, and using Ampure beads (Beckman-Coulter) for size selection and purification using a bead ratio of 0.8:1 beads:library. .. PCR amplicons were barcoded and sent for 454 Titanium pyrosequencing (Roche, Basel, Switzerland) at the Génome Québec Innovation Centre at the McGill University (Montreal, QC, Canada).

    Ligation:

    Article Title: Correction: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA
    Article Snippet: .. Sequin-Orland et al. 2013 using the NEBNext Quick DNA Library Prep Master Mix Set for 454 (New England BioLabs, ref E6090 for A-tailed (AT) ligation of forked Illumina adapters and the NEBNext DNA Library Prep Master Mix Set for 454 (New England BioLabs, ref E6070) for blunt-end (BE ) ligation of Illumina adapters without forked shape. ..

    Construct:

    Article Title: CoproID predicts the source of coprolites and paleofeces using microbiome composition and host DNA content
    Article Snippet: .. Library preparation and Sequencing For paleofeces and sediment samples, double-stranded, dual-indexed shotgun Illumina libraries were constructed following ( ) using either the NEBNext DNA Library Prep Master Set (E6070) kit ( ; ) for the Mexican paleofeces or individually purchased reagents ( ) for all other samples. .. Following library amplification using Phusion HotStart II (Mexican paleofeces) or Agilent Pfu Turbo Cx Hotstart (all other paleofeces) polymerase, the libraries were purified using a Qiagen MinElute PCR Purification kit and quantified using either a BioAnalyzer 2100 with High Sensitivity DNA reagents or an Agilent Tape Station D1000 Screen Tape kit.

    Purification:

    Article Title: High temporal and spatial diversity in marine RNA viruses implies that they have an important role in mortality and structuring plankton communities
    Article Snippet: .. Library prep and pyrosequencing Libraries for each site were prepared for sequencing using NEBNext DNA Library Prep for 454 kit (New England Biolabs, Ipswich, Massachusetts, USA) following the manufacturer's directions, and using Ampure beads (Beckman-Coulter) for size selection and purification using a bead ratio of 0.8:1 beads:library. .. PCR amplicons were barcoded and sent for 454 Titanium pyrosequencing (Roche, Basel, Switzerland) at the Génome Québec Innovation Centre at the McGill University (Montreal, QC, Canada).

    Sequencing:

    Article Title: High temporal and spatial diversity in marine RNA viruses implies that they have an important role in mortality and structuring plankton communities
    Article Snippet: .. Library prep and pyrosequencing Libraries for each site were prepared for sequencing using NEBNext DNA Library Prep for 454 kit (New England Biolabs, Ipswich, Massachusetts, USA) following the manufacturer's directions, and using Ampure beads (Beckman-Coulter) for size selection and purification using a bead ratio of 0.8:1 beads:library. .. PCR amplicons were barcoded and sent for 454 Titanium pyrosequencing (Roche, Basel, Switzerland) at the Génome Québec Innovation Centre at the McGill University (Montreal, QC, Canada).

    Article Title: CoproID predicts the source of coprolites and paleofeces using microbiome composition and host DNA content
    Article Snippet: .. Library preparation and Sequencing For paleofeces and sediment samples, double-stranded, dual-indexed shotgun Illumina libraries were constructed following ( ) using either the NEBNext DNA Library Prep Master Set (E6070) kit ( ; ) for the Mexican paleofeces or individually purchased reagents ( ) for all other samples. .. Following library amplification using Phusion HotStart II (Mexican paleofeces) or Agilent Pfu Turbo Cx Hotstart (all other paleofeces) polymerase, the libraries were purified using a Qiagen MinElute PCR Purification kit and quantified using either a BioAnalyzer 2100 with High Sensitivity DNA reagents or an Agilent Tape Station D1000 Screen Tape kit.

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. The libraries were shotgun-sequenced at the UT Institute of Genomics, Estonian Biocentre core lab with Illumina NextSeq 500 using a 75 bp single-end kit.

    Gel Extraction:

    Article Title: Mitochondrial Phylogenomics of Modern and Ancient Equids
    Article Snippet: .. We selected fragments of 200–300 bp using the EZNA Gel Extraction Kit (Omega Bio-Tek, ref: D2500) and converted each sample into an Illumina library using the NEBNext Quick DNA library prep Master mix set for 454 (New England BioLabs, ref: E6090). .. We amplified the libraries in a 50 µl volume reaction, with two parallel PCR set-ups per library using half (16 µl) of each DNA library.

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  • 99
    New England Biolabs nebnext dna library prep master mix set for 454
    Nebnext Dna Library Prep Master Mix Set For 454, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext dna library prep master mix set for 454/product/New England Biolabs
    Average 99 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    nebnext dna library prep master mix set for 454 - by Bioz Stars, 2020-10
    99/100 stars
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