e6070  (New England Biolabs)


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    Name:
    NEBNext DNA Library Prep Master Mix Set for 454
    Description:
    NEBNext DNA Library Prep Master Mix Set for 454 50 rxns
    Catalog Number:
    e6070l
    Price:
    1260
    Size:
    50 rxns
    Category:
    DNA Analysis
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    New England Biolabs e6070
    NEBNext DNA Library Prep Master Mix Set for 454
    NEBNext DNA Library Prep Master Mix Set for 454 50 rxns
    https://www.bioz.com/result/e6070/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e6070 - by Bioz Stars, 2020-02
    95/100 stars

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    Amplification:

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK). .. The libraries were polymerase chain reaction (PCR) amplified with index primers and purified using Qiaquick columns (Qiagen, Hilden, Germany) according to the manufacturer's instruction ( ).

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. For each archaeological sample, three shotgun libraries were completed by PCR amplification using indexed primers and the following DNA polymerases: Platinum Taq High Fidelity Polymerase (Life Technologies), Phusion Hot Start II (Thermo Fischer), and KAPA HiFi Uracil+ (Kapa Biosystems).

    Article Title: Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain
    Article Snippet: Paragraph title: Library Preparation and Amplification. ... Double-stranded libraries were built using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070; New England Biolabs) and Illumina specific adapters from ref. , following the manufacturer’s instructions with minor modifications to the protocol ( ).

    Article Title: Improving access to endogenous DNA in ancient bones and teeth
    Article Snippet: Library preparation and sequencing Blunt-end Illumina sequencing libraries were built following guidelines previously outlined , using the NEBNext® DNA Library Prep Master Mix Set E6070 (New England Biolabs Inc., Manual Version 2.1 . .. The libraries were amplified using a two-round PCR setup with primers containing a 6 bp known index sequence.

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: Paragraph title: Extraction and library amplification: bone ... The DNA extract and negative control were then built into genomic libraries using the NEB E6070 kit (New England Biolabs), following a protocol slightly modified from that by Vilstrup et al. ( ).

    X-ray Spectroscopy:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. Organic and inorganic residue analysis of various embalming materials was carried out at the Institute of Chemistry, UT, using optical microscopy, ATR-FT-IR spectroscopy (attenuated total reflectance—Fourier transform—infrared spectroscopy), GC-MS (gas chromatography—mass spectrometry), ESI-FT-ICR-MS (electrospray ionization—Fourier transform—ion cyclotron resonance—mass spectrometry), and SEM-EDS (scanning electron microscopy—energy dispersive X-ray spectroscopy).

    Construct:

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: .. To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK). .. The libraries were polymerase chain reaction (PCR) amplified with index primers and purified using Qiaquick columns (Qiagen, Hilden, Germany) according to the manufacturer's instruction ( ).

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: .. In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: .. In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Library preparation was modified from the manufacturers’ instructions, decreasing the total volume to 50 µl, and also replacing SPRI bead purification with silica column purification (Qiagen MinElute PCR Purification kit).

    Adsorption:

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: .. In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Real-time Polymerase Chain Reaction:

    Article Title: Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain
    Article Snippet: Double-stranded libraries were built using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070; New England Biolabs) and Illumina specific adapters from ref. , following the manufacturer’s instructions with minor modifications to the protocol ( ). .. Using quantitative PCR (qPCR), we determined the optimal number of cycles required to amplify the samples to get the right amount of DNA necessary for capture experiments (100–500 ng) ( ).

    Incubation:

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. The Bst Polymerase fill‐in reaction was inactivated after incubation by heating to 80°C for 20 min and then freezing overnight.

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: The DNA extract and negative control were then built into genomic libraries using the NEB E6070 kit (New England Biolabs), following a protocol slightly modified from that by Vilstrup et al. ( ). .. Adapter fill-in reaction was then performed on the flowthrough, before final incubation at 37 °C (30 min) followed by inactivation overnight at − 20 °C.

    Modification:

    Article Title: The Genomic Footprints of the Fall and Recovery of the Crested Ibis
    Article Snippet: .. This DNA was converted into Illumina sequencing libraries through blunt-end ligation using the NEB E6070 kit and the slightly modified version of the protocol of Vilstrup et al. [ ], reported by Paijmans et al. [ ]. ..

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Library preparation was modified from the manufacturers’ instructions, decreasing the total volume to 50 µl, and also replacing SPRI bead purification with silica column purification (Qiagen MinElute PCR Purification kit).

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: .. The DNA extract and negative control were then built into genomic libraries using the NEB E6070 kit (New England Biolabs), following a protocol slightly modified from that by Vilstrup et al. ( ). .. Briefly, extract (30 µl) was end-repaired and then passed through a MinElute column (Qiagen).

    Flow Cytometry:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: The DNA extract and negative control were then built into genomic libraries using the NEB E6070 kit (New England Biolabs), following a protocol slightly modified from that by Vilstrup et al. ( ). .. The collected flow-through was then adapter-ligated and passed through a QiaQuick column (Qiagen).

    Ligation:

    Article Title: The Genomic Footprints of the Fall and Recovery of the Crested Ibis
    Article Snippet: .. This DNA was converted into Illumina sequencing libraries through blunt-end ligation using the NEB E6070 kit and the slightly modified version of the protocol of Vilstrup et al. [ ], reported by Paijmans et al. [ ]. ..

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Article Title: The Arrival of Siberian Ancestry Connecting the Eastern Baltic to Uralic Speakers Further East
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors [ ] following established protocols [ – ]. .. The adaptor ligation module was implemented using 10 μl of buffer, 5 μl of T4 ligase and 5 μl of adaptor mix [ ], incubating at 20 °C for 15 minutes.

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Blunt end adapters (IS1/IS3 and IS3/IS2) were prepared and used for ligation at a final concentration of 0.6 µM in a final volume of 50 µl.

    Generated:

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: Mitogenome sequencing, assembly, and annotation Mitogenomes were generated using several approaches. .. To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK).

    Sequencing:

    Article Title: Draft Genome Sequence of the First Human Isolate of the Ruminant Pathogen Mycoplasma capricolum subsp. capricolum
    Article Snippet: Here, we report the draft genome sequence of the human Mycoplasma capricolum subsp. capricolum strain 14DL0024. .. DNA libraries were built using the NEBNext library kit E6070 and sequenced on the Illumina HiSeq 2000 platform, yielding a total of 20,483,838 paired-end reads.

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: .. To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK). .. The libraries were polymerase chain reaction (PCR) amplified with index primers and purified using Qiaquick columns (Qiagen, Hilden, Germany) according to the manufacturer's instruction ( ).

    Article Title: The Genomic Footprints of the Fall and Recovery of the Crested Ibis
    Article Snippet: .. This DNA was converted into Illumina sequencing libraries through blunt-end ligation using the NEB E6070 kit and the slightly modified version of the protocol of Vilstrup et al. [ ], reported by Paijmans et al. [ ]. ..

    Article Title: Target-Dependent Enrichment of Virions Determines the Reduction of High-Throughput Sequencing in Virus Discovery
    Article Snippet: The low quantities of reads emphasize the need for deep sequencing and/or implementation of target enrichment procedures. .. For total DNA libraries, the NEBNext E6070 was selected for its ability to include large amounts of DNA, increasing library complexity and the possibility to obtain viral reads.

    Article Title: Interspecific Gene Flow Shaped the Evolution of the Genus Canis
    Article Snippet: Paragraph title: Whole-genome sequencing ... All extracts were incorporated into double-stranded DNA libraries build using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070 - New England Biolabs, Beverly, MA, USA) following the manufacturer’s protocol and Illumina-compatible adapters [ ].

    Article Title: Target-Dependent Enrichment of Virions Determines the Reduction of High-Throughput Sequencing in Virus Discovery
    Article Snippet: .. Library preparation and sequencing Three different library preparation kits were used; NEBNext E6070 (New England BioLabs) for total DNA, Nextera XT DNA Sample preparation kit for the enriched virion-associated DNA (Illumina), and ScriptSeq v2 (Epicentre) for total RNA and enriched virion-associated RNA. ..

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. The libraries were shotgun-sequenced at the UT Institute of Genomics, Estonian Biocentre core lab with Illumina NextSeq 500 using a 75 bp single-end kit.

    Article Title: The Arrival of Siberian Ancestry Connecting the Eastern Baltic to Uralic Speakers Further East
    Article Snippet: .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors [ ] following established protocols [ – ]. ..

    Article Title: Improving access to endogenous DNA in ancient bones and teeth
    Article Snippet: .. Library preparation and sequencing Blunt-end Illumina sequencing libraries were built following guidelines previously outlined , using the NEBNext® DNA Library Prep Master Mix Set E6070 (New England Biolabs Inc., Manual Version 2.1 . .. The libraries were amplified using a two-round PCR setup with primers containing a 6 bp known index sequence.

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: HTS library construction Illumina sequencing libraries were prepared for a total of fifteen samples (Table ). .. In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs).

    Computed Tomography:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: For the archaeothanatological study, based on the precepts laid out by Duday [ ], CT scan images identifying bone lateralization and position, combined with visual observations and taphonomic aspects, were taken into account to reconstruct the initial burial practices and the treatment of the body during and after the mummification process. .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ].

    Binding Assay:

    Article Title: Interspecific Gene Flow Shaped the Evolution of the Genus Canis
    Article Snippet: The supernatant was then mixed 1:10 with a binding buffer following [ ] in a binding apparatus following [ ], including a Minelute column (QIAGEN, Hilden, Germany) that was then washed and DNA was eluted according to the manufacturer’s guidelines. .. All extracts were incorporated into double-stranded DNA libraries build using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070 - New England Biolabs, Beverly, MA, USA) following the manufacturer’s protocol and Illumina-compatible adapters [ ].

    Isolation:

    Article Title: Draft Genome Sequence of the First Human Isolate of the Ruminant Pathogen Mycoplasma capricolum subsp. capricolum
    Article Snippet: Recently, the Mycoplasma capricolum subsp. capricolum strain 14DL0024 was isolated from a hospitalized human displaying symptoms of septicemia (M. Heller, R. Schwarz, G. Noe, J. Jores, A. Fischer, E. Schubert, and K. Sachse, submitted for publication). .. DNA libraries were built using the NEBNext library kit E6070 and sequenced on the Illumina HiSeq 2000 platform, yielding a total of 20,483,838 paired-end reads.

    Microscopy:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. Organic and inorganic residue analysis of various embalming materials was carried out at the Institute of Chemistry, UT, using optical microscopy, ATR-FT-IR spectroscopy (attenuated total reflectance—Fourier transform—infrared spectroscopy), GC-MS (gas chromatography—mass spectrometry), ESI-FT-ICR-MS (electrospray ionization—Fourier transform—ion cyclotron resonance—mass spectrometry), and SEM-EDS (scanning electron microscopy—energy dispersive X-ray spectroscopy).

    Purification:

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK). .. The libraries were polymerase chain reaction (PCR) amplified with index primers and purified using Qiaquick columns (Qiagen, Hilden, Germany) according to the manufacturer's instruction ( ).

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: .. In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Article Title: The Arrival of Siberian Ancestry Connecting the Eastern Baltic to Uralic Speakers Further East
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors [ ] following established protocols [ – ]. .. The samples were purified using 500 μl PB and 650 μl of PE buffer and eluted in 30 μl EB buffer (MinElute PCR Purification Kit, QIAGEN).

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Library preparation was modified from the manufacturers’ instructions, decreasing the total volume to 50 µl, and also replacing SPRI bead purification with silica column purification (Qiagen MinElute PCR Purification kit).

    Polymerase Chain Reaction:

    Article Title: An expanded mammal mitogenome dataset from Southeast Asia
    Article Snippet: To construct libraries, DNA was sheared to the target size range using Bioruptor® XL (Diagenode, USA [Diagenode, RRID:SCR_014807]) and converted into an Illumina-compatible sequencing library using the NEBNext E6070 Kit (New England Biolabs, UK). .. The libraries were polymerase chain reaction (PCR) amplified with index primers and purified using Qiaquick columns (Qiagen, Hilden, Germany) according to the manufacturer's instruction ( ).

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: .. In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Article Title: The Arrival of Siberian Ancestry Connecting the Eastern Baltic to Uralic Speakers Further East
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors [ ] following established protocols [ – ]. .. The samples were purified using 500 μl PB and 650 μl of PE buffer and eluted in 30 μl EB buffer (MinElute PCR Purification Kit, QIAGEN).

    Article Title: Improving access to endogenous DNA in ancient bones and teeth
    Article Snippet: Library preparation and sequencing Blunt-end Illumina sequencing libraries were built following guidelines previously outlined , using the NEBNext® DNA Library Prep Master Mix Set E6070 (New England Biolabs Inc., Manual Version 2.1 . .. The libraries were amplified using a two-round PCR setup with primers containing a 6 bp known index sequence.

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Library preparation was modified from the manufacturers’ instructions, decreasing the total volume to 50 µl, and also replacing SPRI bead purification with silica column purification (Qiagen MinElute PCR Purification kit).

    Spectroscopy:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. Organic and inorganic residue analysis of various embalming materials was carried out at the Institute of Chemistry, UT, using optical microscopy, ATR-FT-IR spectroscopy (attenuated total reflectance—Fourier transform—infrared spectroscopy), GC-MS (gas chromatography—mass spectrometry), ESI-FT-ICR-MS (electrospray ionization—Fourier transform—ion cyclotron resonance—mass spectrometry), and SEM-EDS (scanning electron microscopy—energy dispersive X-ray spectroscopy).

    Shotgun Sequencing:

    Article Title: Target-Dependent Enrichment of Virions Determines the Reduction of High-Throughput Sequencing in Virus Discovery
    Article Snippet: Obtaining a low number of RNA sequences is not unusual when using RNA shotgun sequencing. .. For total DNA libraries, the NEBNext E6070 was selected for its ability to include large amounts of DNA, increasing library complexity and the possibility to obtain viral reads.

    Negative Control:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: .. The DNA extract and negative control were then built into genomic libraries using the NEB E6070 kit (New England Biolabs), following a protocol slightly modified from that by Vilstrup et al. ( ). .. Briefly, extract (30 µl) was end-repaired and then passed through a MinElute column (Qiagen).

    Sample Prep:

    Article Title: Mitochondrial DNA from the eradicated European Plasmodium vivax and P. falciparum from 70-year-old slides from the Ebro Delta in Spain
    Article Snippet: .. Double-stranded libraries were built using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070; New England Biolabs) and Illumina specific adapters from ref. , following the manufacturer’s instructions with minor modifications to the protocol ( ). .. Using quantitative PCR (qPCR), we determined the optimal number of cycles required to amplify the samples to get the right amount of DNA necessary for capture experiments (100–500 ng) ( ).

    Article Title: Interspecific Gene Flow Shaped the Evolution of the Genus Canis
    Article Snippet: .. All extracts were incorporated into double-stranded DNA libraries build using the NEBNext DNA Sample Prep Master Mix Set 2 (E6070 - New England Biolabs, Beverly, MA, USA) following the manufacturer’s protocol and Illumina-compatible adapters [ ]. .. Libraries were sequenced using 50 base pair single (Golden jackal Calcutta, Hunting dog Kenya 1 and Hunting dog Somalia) or 100 base pair paired end (remaining samples) read chemistry on Illumina HiSeq 2000 and 2500 (Illumina, San Diego, CA, USA) platforms.

    Article Title: Target-Dependent Enrichment of Virions Determines the Reduction of High-Throughput Sequencing in Virus Discovery
    Article Snippet: .. Library preparation and sequencing Three different library preparation kits were used; NEBNext E6070 (New England BioLabs) for total DNA, Nextera XT DNA Sample preparation kit for the enriched virion-associated DNA (Illumina), and ScriptSeq v2 (Epicentre) for total RNA and enriched virion-associated RNA. ..

    Next-Generation Sequencing:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Ancient DNA (aDNA) extraction was performed in a dedicated aDNA laboratory at the UT, Institute of Ecology and Earth Sciences, Estonia (see for details), and sequenced with the Next-generation Sequencing technology on Illumina platform. .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ].

    Ancient DNA Assay:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Ancient DNA (aDNA) extraction was performed in a dedicated aDNA laboratory at the UT, Institute of Ecology and Earth Sciences, Estonia (see for details), and sequenced with the Next-generation Sequencing technology on Illumina platform. .. Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ].

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: All initial library preparation steps were performed in the University of Oklahoma’s LMAMR dedicated ancient DNA facility following previously published protocols , with minor modifications. .. In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs).

    Concentration Assay:

    Article Title: The Genomic Footprints of the Fall and Recovery of the Crested Ibis
    Article Snippet: After concentration to sub 1ml volumes, concentrates were passed through MinElute columns (QIAGEN®, Venlo, Netherlands) according to the manufacturer’s protocol, and finally eluted in 60μl EB buffer. .. This DNA was converted into Illumina sequencing libraries through blunt-end ligation using the NEB E6070 kit and the slightly modified version of the protocol of Vilstrup et al. [ ], reported by Paijmans et al. [ ].

    Article Title: Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus
    Article Snippet: In brief, for each sample, 100 ng of DNA (or 30 μl of sample extract for low‐yield dentine and control samples) was constructed into indexed libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs) according to manufacturer instructions, except that SPRI bead purification was replaced with silica adsorption (Qiagen MinElute PCR Purification kit). .. Blunt end adapters (IS1/IS3 and IS2/IS3) were prepared following Meyer and Kircher ( ) and used for ligation at a concentration of 0.5 μM in a final volume of 50 µl.

    Article Title: Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
    Article Snippet: In brief, up to 30 μl of sample extract (to a maximum of 100 ng input DNA) (Supplementary Table ) was constructed into Illumina libraries using the NEBNext DNA Library Prep Master set for 454 (E6070; New England Biolabs). .. Blunt end adapters (IS1/IS3 and IS3/IS2) were prepared and used for ligation at a final concentration of 0.6 µM in a final volume of 50 µl.

    Gas Chromatography-Mass Spectrometry:

    Article Title: Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods)
    Article Snippet: Sequencing libraries were built using NEBNext DNA Library Prep Master Mix Set for 454 (E6070, New England Biolabs) and Illumina-specific adaptors, following specific protocols [ ]. .. Organic and inorganic residue analysis of various embalming materials was carried out at the Institute of Chemistry, UT, using optical microscopy, ATR-FT-IR spectroscopy (attenuated total reflectance—Fourier transform—infrared spectroscopy), GC-MS (gas chromatography—mass spectrometry), ESI-FT-ICR-MS (electrospray ionization—Fourier transform—ion cyclotron resonance—mass spectrometry), and SEM-EDS (scanning electron microscopy—energy dispersive X-ray spectroscopy).

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    New England Biolabs dna library prep master mix
    Dna Library Prep Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna library prep master mix/product/New England Biolabs
    Average 95 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    dna library prep master mix - by Bioz Stars, 2020-02
    95/100 stars
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