fragmented dna  (New England Biolabs)


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  • 93
    Name:
    NEBNext Quick Ligation Module
    Description:
    NEBNext Quick Ligation Module 100 rxns
    Catalog Number:
    e6056l
    Price:
    1248
    Size:
    100 rxns
    Category:
    DNA Template Preparation for PCR
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    Structured Review

    New England Biolabs fragmented dna
    NEBNext Quick Ligation Module
    NEBNext Quick Ligation Module 100 rxns
    https://www.bioz.com/result/fragmented dna/product/New England Biolabs
    Average 93 stars, based on 643 article reviews
    Price from $9.99 to $1999.99
    fragmented dna - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Multiplexing:

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Multiplex Assay:

    Article Title: Whole Genome Sequence-Based Analysis of a Model Complex Trait, High Density Lipoprotein Cholesterol
    Article Snippet: .. Illumina multiplex adapter ligation (NEBNext Quick Ligation Module Cat. No. E6056L) was performed in a total reaction volume of 90ul containing 18ul 5− buffer, 5ul ligase, 0.5ul 100uM adaptor and H2O at room temperature for 30 minutes. .. After ligation, PCR was performed with Illumina PE 1.0 and modified barcode primers in 170µl reactions containing 85 2− Phusion High-Fidelity PCR master mix, adaptor ligated DNA, 1.75ul of 50uM each primer and H2 O.

    Amplification:

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Sample Prep:

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Ligation:

    Article Title: The Escherichia coli transcriptome mostly consists of independently regulated modules
    Article Snippet: .. The sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5′-phosphorylated) using dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs), respectively. .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Article Title: Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples
    Article Snippet: .. We have found using Blunt/TA Ligase instead of the NEBNext Quick Ligation Module as described in the native barcoding protocol improves the efficiency of this step. ..

    Article Title: Whole Genome Sequence-Based Analysis of a Model Complex Trait, High Density Lipoprotein Cholesterol
    Article Snippet: .. Illumina multiplex adapter ligation (NEBNext Quick Ligation Module Cat. No. E6056L) was performed in a total reaction volume of 90ul containing 18ul 5− buffer, 5ul ligase, 0.5ul 100uM adaptor and H2O at room temperature for 30 minutes. .. After ligation, PCR was performed with Illumina PE 1.0 and modified barcode primers in 170µl reactions containing 85 2− Phusion High-Fidelity PCR master mix, adaptor ligated DNA, 1.75ul of 50uM each primer and H2 O.

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: .. Thereafter, adapters were ligated using the NEBNext® Quick Ligation Module (New England Biolabs). .. The final library was purified and quantified and subsequently sequenced on the MinION sequencing device (Oxford Nanopore Technologies) according to the manufacturer’s protocol.

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Article Title: Prophage-Driven Genomic Structural Changes Promote Bartonella Vertical Evolution
    Article Snippet: .. Adapter ligation (BAM) was performed for 15 min using NEBNext Quick Ligation Module (New England Biolabs, MA, USA). .. Library mix was cleaned up using 0.4X AmPure beads (Beckmann Coulter, USA) and library was eluted in 15 µl of elution buffer and used for sequencing.

    Purification:

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Polymerase Chain Reaction:

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Sequencing:

    Article Title: Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences
    Article Snippet: .. NEBNext dA-tailing Module Protocol:Purify on a Minelute PCR Purification Kit (Qiagen 28004) and elute in 25 µl EB.NEBNext Quick Ligation Module Protocol: Purify on a Minelute PCR Purification Kit (Qiagen28004) and elute in 30 µl EB, using the multiplexing sequencing adapters providedin the Multiplexing Sample Preparation Oligo Kit. .. Size selection of the library was doneon a 2% agarose gel (Low Range Ultra Agarose Biorad 161-3107).

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

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  • News
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  • 99
    New England Biolabs nebnext quick ligation module
    Nebnext Quick Ligation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext quick ligation module/product/New England Biolabs
    Average 99 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    nebnext quick ligation module - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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