onetaq one step rt pcr kit  (New England Biolabs)


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  • 96
    Name:
    OneTaq One Step RT PCR Kit
    Description:
    OneTaq One Step RT PCR Kit 30 rxns
    Catalog Number:
    e5315s
    Price:
    164
    Size:
    30 rxns
    Category:
    PCR related Kits
    Buy from Supplier


    Structured Review

    New England Biolabs onetaq one step rt pcr kit
    OneTaq One Step RT PCR Kit
    OneTaq One Step RT PCR Kit 30 rxns
    https://www.bioz.com/result/onetaq one step rt pcr kit/product/New England Biolabs
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    onetaq one step rt pcr kit - by Bioz Stars, 2020-10
    96/100 stars

    Images

    1) Product Images from "A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing"

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing

    Journal: bioRxiv

    doi: 10.1101/2020.04.07.029199

    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.
    Figure Legend Snippet: qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Techniques Used: Amplification, Negative Control, Sequencing, Reverse Transcription Polymerase Chain Reaction, Purification, RNA Extraction

    2) Product Images from "A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing"

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing

    Journal: bioRxiv

    doi: 10.1101/2020.04.07.029199

    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.
    Figure Legend Snippet: qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Techniques Used: Amplification, Negative Control, Sequencing, Reverse Transcription Polymerase Chain Reaction, Purification, RNA Extraction

    Related Articles

    Clone Assay:

    Article Title: HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
    Article Snippet: .. Viral DNA was amplified from viral RNA by RT-PCR using the OneTaq one-step RT-PCR kit (catalog number E5315S; NEB) and cloned using the NEB PCR cloning kit (catalog number E1203S; NEB). .. On average, 40 or more DNA clones for each RT-PCR product were prepared and sequenced by MCLAB (San Francisco, CA).

    Amplification:

    Article Title: HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
    Article Snippet: .. Viral DNA was amplified from viral RNA by RT-PCR using the OneTaq one-step RT-PCR kit (catalog number E5315S; NEB) and cloned using the NEB PCR cloning kit (catalog number E1203S; NEB). .. On average, 40 or more DNA clones for each RT-PCR product were prepared and sequenced by MCLAB (San Francisco, CA).

    Article Title: Live bird markets as evolutionary epicentres of H9N2 low pathogenicity avian influenza viruses in Korea
    Article Snippet: .. All eight segments of cDNA were synthesized and amplified via reverse transcription PCR using the OneTaq® One-Step RT–PCR Kit (New England Biolabs) [ ]. .. The Nextera XT DNA Sample Preparation Kit (Illumina, USA) was used to generate multiplexed paired-end sequencing libraries according to the manufacturer's instructions.

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing
    Article Snippet: .. qSanger Amplification Reverse transcription and amplification for was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). .. Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat. # E3005E) were used for . was performed exclusively with the Luna Universal One-step RT-qPCR Kit.

    Synthesized:

    Article Title: Live bird markets as evolutionary epicentres of H9N2 low pathogenicity avian influenza viruses in Korea
    Article Snippet: .. All eight segments of cDNA were synthesized and amplified via reverse transcription PCR using the OneTaq® One-Step RT–PCR Kit (New England Biolabs) [ ]. .. The Nextera XT DNA Sample Preparation Kit (Illumina, USA) was used to generate multiplexed paired-end sequencing libraries according to the manufacturer's instructions.

    Quantitative RT-PCR:

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing
    Article Snippet: .. Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat. # E3005E) were used for . was performed exclusively with the Luna Universal One-step RT-qPCR Kit. .. All reactions contained Tween-20 at a final concentration of 1% v/v, 500 nM final concentration of each amplification primer, and 100 GCE of synthetic dsDNA spike-in molecules.

    Polymerase Chain Reaction:

    Article Title: Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube
    Article Snippet: .. In situ hybridization was carried out as previously described , using the following probes: foxa2 cDNA was PCR-amplified from cDNA prepared from 1-dpf embryos using the OneTaq One step RT-PCR kit (NEB) and the following primers: 5’-CCT TGA CAG GAG GAG AGC AC-3’ and 5’-AAA AGC CGC CTT GAA GAG TT-3’ ; The resulting PCR fragment was TA-cloned into the pGEM-T Easy vector (Promega). .. Antisense digoxigenin- or fluorescein-labeled RNA probes were transcribed using the MAXIscript kit (Ambion) and WISH was carried out as previously described ( ).

    Article Title: HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
    Article Snippet: .. Viral DNA was amplified from viral RNA by RT-PCR using the OneTaq one-step RT-PCR kit (catalog number E5315S; NEB) and cloned using the NEB PCR cloning kit (catalog number E1203S; NEB). .. On average, 40 or more DNA clones for each RT-PCR product were prepared and sequenced by MCLAB (San Francisco, CA).

    Article Title: Gene expression studies using a miniaturized thermal cycler system on board the International Space Station
    Article Snippet: .. Semi-quantitative PCR For the combined one-step RT-PCR, 100 ng of RNA from each condition was added to a tube and combined with OneTaq One-Step RT-PCR mix (NEB, E5315S) and 0.4 μM of each primer ( ). ..

    Article Title: Live bird markets as evolutionary epicentres of H9N2 low pathogenicity avian influenza viruses in Korea
    Article Snippet: .. All eight segments of cDNA were synthesized and amplified via reverse transcription PCR using the OneTaq® One-Step RT–PCR Kit (New England Biolabs) [ ]. .. The Nextera XT DNA Sample Preparation Kit (Illumina, USA) was used to generate multiplexed paired-end sequencing libraries according to the manufacturer's instructions.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives
    Article Snippet: .. One or 4 μL RNA from FFPE midbrain of animal 15375 or muskoxen faecal samples, respectively, were tested using the OneTaq One-Step RT-PCR Kit (New England Biolabs, Ipswich, MA) using the alternative protocol described by the manufacturer. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube
    Article Snippet: .. In situ hybridization was carried out as previously described , using the following probes: foxa2 cDNA was PCR-amplified from cDNA prepared from 1-dpf embryos using the OneTaq One step RT-PCR kit (NEB) and the following primers: 5’-CCT TGA CAG GAG GAG AGC AC-3’ and 5’-AAA AGC CGC CTT GAA GAG TT-3’ ; The resulting PCR fragment was TA-cloned into the pGEM-T Easy vector (Promega). .. Antisense digoxigenin- or fluorescein-labeled RNA probes were transcribed using the MAXIscript kit (Ambion) and WISH was carried out as previously described ( ).

    Article Title: HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site
    Article Snippet: .. Viral DNA was amplified from viral RNA by RT-PCR using the OneTaq one-step RT-PCR kit (catalog number E5315S; NEB) and cloned using the NEB PCR cloning kit (catalog number E1203S; NEB). .. On average, 40 or more DNA clones for each RT-PCR product were prepared and sequenced by MCLAB (San Francisco, CA).

    Article Title: Gene expression studies using a miniaturized thermal cycler system on board the International Space Station
    Article Snippet: .. Semi-quantitative PCR For the combined one-step RT-PCR, 100 ng of RNA from each condition was added to a tube and combined with OneTaq One-Step RT-PCR mix (NEB, E5315S) and 0.4 μM of each primer ( ). ..

    Article Title: Live bird markets as evolutionary epicentres of H9N2 low pathogenicity avian influenza viruses in Korea
    Article Snippet: .. All eight segments of cDNA were synthesized and amplified via reverse transcription PCR using the OneTaq® One-Step RT–PCR Kit (New England Biolabs) [ ]. .. The Nextera XT DNA Sample Preparation Kit (Illumina, USA) was used to generate multiplexed paired-end sequencing libraries according to the manufacturer's instructions.

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing
    Article Snippet: .. qSanger Amplification Reverse transcription and amplification for was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). .. Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat. # E3005E) were used for . was performed exclusively with the Luna Universal One-step RT-qPCR Kit.

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing
    Article Snippet: .. Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat. # E3005E) were used for . was performed exclusively with the Luna Universal One-step RT-qPCR Kit. .. All reactions contained Tween-20 at a final concentration of 1% v/v, 500 nM final concentration of each amplification primer, and 100 GCE of synthetic dsDNA spike-in molecules.

    Article Title: A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria
    Article Snippet: .. Eight primers pairs were designed using Primer 3 (2.3.7) tool in Geneious (Table ) to confirm the physical presence of all eight viral segments using RT-PCR (OneTaq One-Step RT-PCR Kit; NEB) [ ] on fresh RNA extracts from N. benthamiana . .. The amplicons were gel-purified using Zymoclean Gel DNA Recovery Kit (Zymo Research) and Sanger sequenced; sequence analyses of these amplicons showed that they were 100% identical to the corresponding segment sequences obtained by the HTS analysis and thus confirmed the presence of each individual viral segment.

    Article Title: Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives
    Article Snippet: .. One or 4 μL RNA from FFPE midbrain of animal 15375 or muskoxen faecal samples, respectively, were tested using the OneTaq One-Step RT-PCR Kit (New England Biolabs, Ipswich, MA) using the alternative protocol described by the manufacturer. ..

    In Situ Hybridization:

    Article Title: Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube
    Article Snippet: .. In situ hybridization was carried out as previously described , using the following probes: foxa2 cDNA was PCR-amplified from cDNA prepared from 1-dpf embryos using the OneTaq One step RT-PCR kit (NEB) and the following primers: 5’-CCT TGA CAG GAG GAG AGC AC-3’ and 5’-AAA AGC CGC CTT GAA GAG TT-3’ ; The resulting PCR fragment was TA-cloned into the pGEM-T Easy vector (Promega). .. Antisense digoxigenin- or fluorescein-labeled RNA probes were transcribed using the MAXIscript kit (Ambion) and WISH was carried out as previously described ( ).

    Plasmid Preparation:

    Article Title: Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube
    Article Snippet: .. In situ hybridization was carried out as previously described , using the following probes: foxa2 cDNA was PCR-amplified from cDNA prepared from 1-dpf embryos using the OneTaq One step RT-PCR kit (NEB) and the following primers: 5’-CCT TGA CAG GAG GAG AGC AC-3’ and 5’-AAA AGC CGC CTT GAA GAG TT-3’ ; The resulting PCR fragment was TA-cloned into the pGEM-T Easy vector (Promega). .. Antisense digoxigenin- or fluorescein-labeled RNA probes were transcribed using the MAXIscript kit (Ambion) and WISH was carried out as previously described ( ).

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  • 96
    New England Biolabs onetaq one step rt pcr kit
    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to <t>RT-PCR</t> master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and <t>OneTaq</t> polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.
    Onetaq One Step Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq one step rt pcr kit/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    onetaq one step rt pcr kit - by Bioz Stars, 2020-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Journal: bioRxiv

    Article Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing

    doi: 10.1101/2020.04.07.029199

    Figure Lengend Snippet: qSanger detects SARS-COV-2 RNA when amplified directly from viral particles in transport medium. ( A ) A total of 32 no-template controls, 32 negative control samples (Seracare) and 32 positive samples (Seracare) were assayed. All results were concordant with Seracare and NTC. Three samples were no-calls (undetermined) due to low signal-to-noise ratio in the sequencing results. ( B ) Positive Seracare samples were added to RT-PCR master mix either directly from the VTM or after purification with RNA extraction kit at 125 GCE. The ratio of reference and spike-in intensities were measured by custom data analysis. The mean qSanger ratio was 0.745 (± 0.043 s.e.m., n=8) for direct addition, and 0.97 (± 0.041 s.e.m., n=8) for purified. The coefficient of variation (CV) of positive seracare samples were measured for both Luna and OneTaq polymerase mixes. The CV for Luna direct VTM was 16.4% (n=8), and for Luna purified was 12.1% (n=8). This is consistent with the theoretical counting noise associated with quantifying ~100 molecules.

    Article Snippet: qSanger Amplification Reverse transcription and amplification for was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S).

    Techniques: Amplification, Negative Control, Sequencing, Reverse Transcription Polymerase Chain Reaction, Purification, RNA Extraction