pcr  (New England Biolabs)


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    Name:
    LongAmp Taq PCR Kit
    Description:
    LongAmp Taq PCR Kit 100 rxns
    Catalog Number:
    e5200s
    Price:
    117
    Category:
    Long distance PCR Kits
    Size:
    100 rxns
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    Structured Review

    New England Biolabs pcr
    LongAmp Taq PCR Kit
    LongAmp Taq PCR Kit 100 rxns
    https://www.bioz.com/result/pcr/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells
    Article Snippet: Each sample had RNA removed with the addition of 1ul Rnase H, incubated at 37°C for 30 minutes. .. Subsequent PCR was performed using Long Amp Taq PCR Kit (NEB, E5200S) following the manufacturer’s protocol on 2.5ul cDNA in a 25ul final volume (94°C 30s, 34x (94°C 30 s, 55°C 30s, 65°C 5 m), 65°C 10 m). .. See the Additional file : Table S3 for PCR primers used.

    Article Title: Estrogen-Responsive Genes Encoding Egg Yolk Proteins Vitellogenin and Apolipoprotein II in Chicken are differentially regulated by Selective Estrogen Receptor Modulators
    Article Snippet: .. First strand cDNA Synthesis and PCR amplification were performed using ProtoScript AMV LongAmp Taq RT-PCR kit (New England Biolabs, MA). ..

    Article Title: Identification and analysis of oxygen responsive microRNAs in the root of wild tomato (S. habrochaites)
    Article Snippet: In brief, specific STTM primers were designed and synthesized. .. PCR was carried out by using LongAmp, dNTPs, and LongAmp buffer from New England BioLabsInc (NEB). .. STTM fragment was inserted into the pOT2-Poly-Cis vector firstly by PCR, then through SwaI digestion (NEB), and lastly via T4 DNA ligase (NEB) catalyzed ligation.

    Article Title: Deficiency in Origin Licensing Proteins Impairs Cilia Formation: Implications for the Aetiology of Meier-Gorlin Syndrome
    Article Snippet: Aggregate sizes were measured using light microscope images (40× magnification) using Adobe Photoshop (arbitrary units, lower cut-off point at the single cell size approximately). .. Semi-quantitative RT-PCR (26 cycles) for VEGFA was performed using the ProtoScript AMV LongAmp Taq RT-PCR Kit (New England Biolabs) using the following primer sets: VEGFA: Forward: 5′-GTCTTGGGTGCATTGGAGCC-3′ Reverse: 5′-CCTCGGCTTGTCACATCTGC-3′ ELP4: Forward: 5′-AAGAGGATCCTGCCAACATTT-3′ Reverse: 5′-AGGATTGGATCCATCAAATCC-3′ qRT-PCR for COL1A1 analysis was carried out using the QuantiFast SYBR Green PCR Kit and the following QuantiTect Primers (Qiagen): COL1A1 (NM_000088): Hs_COL1A1_1_SG (cat no. QT0037793). .. GAPDH (NM_002046): Hs_GAPDH_1_SG (cat no. QT00079247) Reactions containing 12.5 µl SYBR Green PCR Master Mix, 2.5 µl 10× Primer assay mix, 5 µl RNAse-free water and 5 µl template cDNA to a final volume of 25 µl were prepared in duplicate.

    Article Title: HIV Gene Expression from Intact Proviruses Positioned in Bacterial Artificial Chromosomes at Integration Sites Previously Identified in Latently Infected T cells
    Article Snippet: The primers used for the host cell promoters and listed in and were used in conjunction with the HIV 5′ primer used to PCR the 500 bp of the BAC upstream of the integration site. .. The conditions for PCR were as per manufacturers instructions for long-amp PCR (New England Biolabs). .. All PCR products were electrophoresed in 0.8% agarose and visualized with ethidium bromide staining.

    Article Title: Application of long-read sequencing for robust identification of correct alleles in genome edited animals
    Article Snippet: PCR was performed with tailed-end primers using the same conditions as for amplicons produced for Sanger sequencing, to generate amplicons for ONT sequencing. .. PCR amplicons were barcoded employing LongAmp Taq (New England BioLabs® ). .. The ends of pooled DNA fragments were repaired employing the NEBNext End repair / dA-tailing Module (New England BioLabs®).

    Amplification:

    Article Title: Antagonistic Effects of Cellular Poly(C) Binding Proteins on Vesicular Stomatitis Virus Gene Expression ▿
    Article Snippet: VSV P with a Flag tag at the amino (N) terminus was cloned into the pHygeGFP vector. .. Coding sequences of PCBP1 ( ) and PCBP2 ( ) were amplified using total RNA recovered from HEK293 cells by using a LongAmp kit (New England BioLabs). .. The reverse transcription-PCRs (RT-PCRs) for PCBP1 amplification were performed using the forward primer NheI-HA-KpnI-PCBP1F containing two restriction sites flanking the sequence for the HA epitope tag (shown in italics) and the reverse primer EcoRI-PCBP1R ( ).

    Article Title: Estrogen-Responsive Genes Encoding Egg Yolk Proteins Vitellogenin and Apolipoprotein II in Chicken are differentially regulated by Selective Estrogen Receptor Modulators
    Article Snippet: .. First strand cDNA Synthesis and PCR amplification were performed using ProtoScript AMV LongAmp Taq RT-PCR kit (New England Biolabs, MA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Estrogen-Responsive Genes Encoding Egg Yolk Proteins Vitellogenin and Apolipoprotein II in Chicken are differentially regulated by Selective Estrogen Receptor Modulators
    Article Snippet: .. First strand cDNA Synthesis and PCR amplification were performed using ProtoScript AMV LongAmp Taq RT-PCR kit (New England Biolabs, MA). ..

    Article Title: Deficiency in Origin Licensing Proteins Impairs Cilia Formation: Implications for the Aetiology of Meier-Gorlin Syndrome
    Article Snippet: Aggregate sizes were measured using light microscope images (40× magnification) using Adobe Photoshop (arbitrary units, lower cut-off point at the single cell size approximately). .. Semi-quantitative RT-PCR (26 cycles) for VEGFA was performed using the ProtoScript AMV LongAmp Taq RT-PCR Kit (New England Biolabs) using the following primer sets: VEGFA: Forward: 5′-GTCTTGGGTGCATTGGAGCC-3′ Reverse: 5′-CCTCGGCTTGTCACATCTGC-3′ ELP4: Forward: 5′-AAGAGGATCCTGCCAACATTT-3′ Reverse: 5′-AGGATTGGATCCATCAAATCC-3′ qRT-PCR for COL1A1 analysis was carried out using the QuantiFast SYBR Green PCR Kit and the following QuantiTect Primers (Qiagen): COL1A1 (NM_000088): Hs_COL1A1_1_SG (cat no. QT0037793). .. GAPDH (NM_002046): Hs_GAPDH_1_SG (cat no. QT00079247) Reactions containing 12.5 µl SYBR Green PCR Master Mix, 2.5 µl 10× Primer assay mix, 5 µl RNAse-free water and 5 µl template cDNA to a final volume of 25 µl were prepared in duplicate.

    Quantitative RT-PCR:

    Article Title: Deficiency in Origin Licensing Proteins Impairs Cilia Formation: Implications for the Aetiology of Meier-Gorlin Syndrome
    Article Snippet: Aggregate sizes were measured using light microscope images (40× magnification) using Adobe Photoshop (arbitrary units, lower cut-off point at the single cell size approximately). .. Semi-quantitative RT-PCR (26 cycles) for VEGFA was performed using the ProtoScript AMV LongAmp Taq RT-PCR Kit (New England Biolabs) using the following primer sets: VEGFA: Forward: 5′-GTCTTGGGTGCATTGGAGCC-3′ Reverse: 5′-CCTCGGCTTGTCACATCTGC-3′ ELP4: Forward: 5′-AAGAGGATCCTGCCAACATTT-3′ Reverse: 5′-AGGATTGGATCCATCAAATCC-3′ qRT-PCR for COL1A1 analysis was carried out using the QuantiFast SYBR Green PCR Kit and the following QuantiTect Primers (Qiagen): COL1A1 (NM_000088): Hs_COL1A1_1_SG (cat no. QT0037793). .. GAPDH (NM_002046): Hs_GAPDH_1_SG (cat no. QT00079247) Reactions containing 12.5 µl SYBR Green PCR Master Mix, 2.5 µl 10× Primer assay mix, 5 µl RNAse-free water and 5 µl template cDNA to a final volume of 25 µl were prepared in duplicate.

    SYBR Green Assay:

    Article Title: Deficiency in Origin Licensing Proteins Impairs Cilia Formation: Implications for the Aetiology of Meier-Gorlin Syndrome
    Article Snippet: Aggregate sizes were measured using light microscope images (40× magnification) using Adobe Photoshop (arbitrary units, lower cut-off point at the single cell size approximately). .. Semi-quantitative RT-PCR (26 cycles) for VEGFA was performed using the ProtoScript AMV LongAmp Taq RT-PCR Kit (New England Biolabs) using the following primer sets: VEGFA: Forward: 5′-GTCTTGGGTGCATTGGAGCC-3′ Reverse: 5′-CCTCGGCTTGTCACATCTGC-3′ ELP4: Forward: 5′-AAGAGGATCCTGCCAACATTT-3′ Reverse: 5′-AGGATTGGATCCATCAAATCC-3′ qRT-PCR for COL1A1 analysis was carried out using the QuantiFast SYBR Green PCR Kit and the following QuantiTect Primers (Qiagen): COL1A1 (NM_000088): Hs_COL1A1_1_SG (cat no. QT0037793). .. GAPDH (NM_002046): Hs_GAPDH_1_SG (cat no. QT00079247) Reactions containing 12.5 µl SYBR Green PCR Master Mix, 2.5 µl 10× Primer assay mix, 5 µl RNAse-free water and 5 µl template cDNA to a final volume of 25 µl were prepared in duplicate.

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  • 94
    New England Biolabs longamp taq
    Longamp Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/longamp taq/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp taq - by Bioz Stars, 2021-03
    94/100 stars
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