e3321  (New England Biolabs)


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  • 94
    Name:
    EnGen Mutation Detection Kit
    Description:
    EnGen Mutation Detection Kit 25 rxns
    Catalog Number:
    e3321s
    Price:
    208
    Size:
    25 rxns
    Category:
    Mutation Detection Kits
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    Structured Review

    New England Biolabs e3321
    EnGen Mutation Detection Kit
    EnGen Mutation Detection Kit 25 rxns
    https://www.bioz.com/result/e3321/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e3321 - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells
    Article Snippet: .. Cell populations with Sox9 mutations were screened by Heteroduplex formation assay using QuickExtract DNA Extraction Solution (QE0905T, Qiagen, Peterborough, UK) and EnGen™ Mutation Detection Kit (E3321S, New England Biolabs, Herts, UK) according to the manufacturer’s instructions. .. We tested four different crRNA and the primer sets to detect mutation within each crRNA-binding region; details are provided in Supplementary Data .

    Amplification:

    Article Title: Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses
    Article Snippet: .. KO mutations at target loci were identified by using the EnGen Mutation Detection Kit (New England Biolabs), and confirmed by sequencing target loci amplified by PCR. .. A double-KO cell line was constructed by transfecting EEPD1 KO #27 cells with pooled Metnase CRISPR vectors as above.

    Tube Formation Assay:

    Article Title: Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells
    Article Snippet: .. Cell populations with Sox9 mutations were screened by Heteroduplex formation assay using QuickExtract DNA Extraction Solution (QE0905T, Qiagen, Peterborough, UK) and EnGen™ Mutation Detection Kit (E3321S, New England Biolabs, Herts, UK) according to the manufacturer’s instructions. .. We tested four different crRNA and the primer sets to detect mutation within each crRNA-binding region; details are provided in Supplementary Data .

    Mutagenesis:

    Article Title: Development of Gene Editing Strategies for Human β-Globin (HBB) Gene Mutations
    Article Snippet: .. T7 Endonuclease AssayThermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). ..

    Article Title: MYC Controls the Epstein-Barr Virus Lytic Switch.
    Article Snippet: .. Epstein-Barr virus (EBV) is associated with multiple human malignancies. .. Epstein-Barr virus (EBV) is associated with multiple human malignancies.

    Article Title: Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells
    Article Snippet: .. Cell populations with Sox9 mutations were screened by Heteroduplex formation assay using QuickExtract DNA Extraction Solution (QE0905T, Qiagen, Peterborough, UK) and EnGen™ Mutation Detection Kit (E3321S, New England Biolabs, Herts, UK) according to the manufacturer’s instructions. .. We tested four different crRNA and the primer sets to detect mutation within each crRNA-binding region; details are provided in Supplementary Data .

    Article Title: Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting
    Article Snippet: .. The T7E1 cleavage assay was performed using an EnGen® Mutation Detection Kit (NEB, Beverly, MA, USA) as previously described[ ] . .. Histology and immunofluorescence analysis The tissues of brainstem from Tph2 -/- pigs and wild-type pigs were fixed with 4% buffered paraformaldehyde for 48 hours.

    Article Title: Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses
    Article Snippet: .. KO mutations at target loci were identified by using the EnGen Mutation Detection Kit (New England Biolabs), and confirmed by sequencing target loci amplified by PCR. .. A double-KO cell line was constructed by transfecting EEPD1 KO #27 cells with pooled Metnase CRISPR vectors as above.

    Detection Assay:

    Article Title: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication
    Article Snippet: .. T7 endonuclease I cleavage detection assay and Sanger sequencing All potential off-target sites with high homology in the sgRNAs were predicted using software CRISPR-offinder . .. Genomic DNAs were extracted using the TIANamp Genomic DNA Kit (TIANGEN) from mutated clonal cells for PCR amplification, and T7 endonuclease I (T7EN I) cleavage detection assay was employed to determine off-target effects.

    Sequencing:

    Article Title: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication
    Article Snippet: .. T7 endonuclease I cleavage detection assay and Sanger sequencing All potential off-target sites with high homology in the sgRNAs were predicted using software CRISPR-offinder . .. Genomic DNAs were extracted using the TIANamp Genomic DNA Kit (TIANGEN) from mutated clonal cells for PCR amplification, and T7 endonuclease I (T7EN I) cleavage detection assay was employed to determine off-target effects.

    Article Title: Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses
    Article Snippet: .. KO mutations at target loci were identified by using the EnGen Mutation Detection Kit (New England Biolabs), and confirmed by sequencing target loci amplified by PCR. .. A double-KO cell line was constructed by transfecting EEPD1 KO #27 cells with pooled Metnase CRISPR vectors as above.

    other:

    Article Title: Meningococcal carriage 7 years after introduction of a serogroup A meningococcal conjugate vaccine in Burkina Faso: results from four cross-sectional carriage surveys
    Article Snippet: The serogroup of each isolate was defined using WGS-based methods, which consisted of characterising the capsule locus for each isolate and investigating each identified capsule gene for genetic variations (eg, mutations, gene deletions, or insertions) to predict the capsule phenotype, as described previously.

    CRISPR:

    Article Title: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication
    Article Snippet: .. T7 endonuclease I cleavage detection assay and Sanger sequencing All potential off-target sites with high homology in the sgRNAs were predicted using software CRISPR-offinder . .. Genomic DNAs were extracted using the TIANamp Genomic DNA Kit (TIANGEN) from mutated clonal cells for PCR amplification, and T7 endonuclease I (T7EN I) cleavage detection assay was employed to determine off-target effects.

    Polymerase Chain Reaction:

    Article Title: Development of Gene Editing Strategies for Human β-Globin (HBB) Gene Mutations
    Article Snippet: .. T7 Endonuclease AssayThermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). ..

    Article Title: Stanniocalcin 2 (STC2) expression promotes post-radiation survival, migration and invasion of nasopharyngeal carcinoma cells
    Article Snippet: .. Selected individual colonies (STC2-KO) were expanded, screened through Western blotting and confirmed by genomic PCR, followed by T7 endonuclease 1 digestion (NEB #E3321). .. Hypoxia and glucose starvation Cells were flushed with a gas mixture of 1% O2 , 5% CO2 , and balanced N2 in hypoxia work stations (3131, Thermo scientific, USA).

    Article Title: Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses
    Article Snippet: .. KO mutations at target loci were identified by using the EnGen Mutation Detection Kit (New England Biolabs), and confirmed by sequencing target loci amplified by PCR. .. A double-KO cell line was constructed by transfecting EEPD1 KO #27 cells with pooled Metnase CRISPR vectors as above.

    Western Blot:

    Article Title: Stanniocalcin 2 (STC2) expression promotes post-radiation survival, migration and invasion of nasopharyngeal carcinoma cells
    Article Snippet: .. Selected individual colonies (STC2-KO) were expanded, screened through Western blotting and confirmed by genomic PCR, followed by T7 endonuclease 1 digestion (NEB #E3321). .. Hypoxia and glucose starvation Cells were flushed with a gas mixture of 1% O2 , 5% CO2 , and balanced N2 in hypoxia work stations (3131, Thermo scientific, USA).

    Software:

    Article Title: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication
    Article Snippet: .. T7 endonuclease I cleavage detection assay and Sanger sequencing All potential off-target sites with high homology in the sgRNAs were predicted using software CRISPR-offinder . .. Genomic DNAs were extracted using the TIANamp Genomic DNA Kit (TIANGEN) from mutated clonal cells for PCR amplification, and T7 endonuclease I (T7EN I) cleavage detection assay was employed to determine off-target effects.

    Cleavage Assay:

    Article Title: Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting
    Article Snippet: .. The T7E1 cleavage assay was performed using an EnGen® Mutation Detection Kit (NEB, Beverly, MA, USA) as previously described[ ] . .. Histology and immunofluorescence analysis The tissues of brainstem from Tph2 -/- pigs and wild-type pigs were fixed with 4% buffered paraformaldehyde for 48 hours.

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  • 94
    New England Biolabs engen mutation detection kit
    Engen Mutation Detection Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen mutation detection kit/product/New England Biolabs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    engen mutation detection kit - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

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