cypridina luciferase assay kits  (New England Biolabs)


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    Name:
    BioLux Cypridina Luciferase Assay Kit
    Description:
    BioLux Cypridina Luciferase Assay Kit 1 000 assays
    Catalog Number:
    e3309l
    Price:
    637
    Size:
    1 000 assays
    Category:
    Cellular Biology
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    Structured Review

    New England Biolabs cypridina luciferase assay kits
    BioLux Cypridina Luciferase Assay Kit
    BioLux Cypridina Luciferase Assay Kit 1 000 assays
    https://www.bioz.com/result/cypridina luciferase assay kits/product/New England Biolabs
    Average 98 stars, based on 293 article reviews
    Price from $9.99 to $1999.99
    cypridina luciferase assay kits - by Bioz Stars, 2020-08
    98/100 stars

    Images

    1) Product Images from "ChREBP Regulates Itself and Metabolic Genes Implicated in Lipid Accumulation in β–Cell Line"

    Article Title: ChREBP Regulates Itself and Metabolic Genes Implicated in Lipid Accumulation in β–Cell Line

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0147411

    Effect of caChREBP on Gpd1 or Rgs16 ChoRE-containing promoters. (A) Alignment of ChoRE sequence presented on Gpd1 promoters among rat (at the position -1943 to -1927), mouse, cow, dog, rhesus, chimpanzee, and human. Sequence assemblies and coordinates are as follows: Rat: Mar.2012 ChrX(+): 115873245–11587361, Mouse: Dec.2011 Chr15(+): 99716109–99716125, Cow: Oct.2011 Chr5(-): 32732081–32732065, Dog: Sep.2011 Chr27(-): 4623314–4623298, Rhesus: Oct.2010 Chr11(+): 47359512–47359528, Chimpanzee: Feb.2011 Chr12(-): 39238594–39238578, and Human: Feb.2009 Chr12(+): 50496575–50496591. Color coding: light grey, identical residues; dark grey, unconserved residues. (B) caChREBP cannot activate Gpd1 ChoRE-containing promoter. We co-transfected luciferase reporter driven by two copies of rat Gpd1 ChoRE (position -1943 to -1927 relative to TSS) upstream of minimal TATA promoter with caChREBP in 832/13 cells in RPMI with 5.5 mmol/l D-glucose. Gaussia luciferase activity was measured at 48h and normalized to Cypridina luciferase, and expressed as fold activation over the activity seen in cells transfected with two copies of ChoRE with minimal TATA promoter and empty vector. *, p
    Figure Legend Snippet: Effect of caChREBP on Gpd1 or Rgs16 ChoRE-containing promoters. (A) Alignment of ChoRE sequence presented on Gpd1 promoters among rat (at the position -1943 to -1927), mouse, cow, dog, rhesus, chimpanzee, and human. Sequence assemblies and coordinates are as follows: Rat: Mar.2012 ChrX(+): 115873245–11587361, Mouse: Dec.2011 Chr15(+): 99716109–99716125, Cow: Oct.2011 Chr5(-): 32732081–32732065, Dog: Sep.2011 Chr27(-): 4623314–4623298, Rhesus: Oct.2010 Chr11(+): 47359512–47359528, Chimpanzee: Feb.2011 Chr12(-): 39238594–39238578, and Human: Feb.2009 Chr12(+): 50496575–50496591. Color coding: light grey, identical residues; dark grey, unconserved residues. (B) caChREBP cannot activate Gpd1 ChoRE-containing promoter. We co-transfected luciferase reporter driven by two copies of rat Gpd1 ChoRE (position -1943 to -1927 relative to TSS) upstream of minimal TATA promoter with caChREBP in 832/13 cells in RPMI with 5.5 mmol/l D-glucose. Gaussia luciferase activity was measured at 48h and normalized to Cypridina luciferase, and expressed as fold activation over the activity seen in cells transfected with two copies of ChoRE with minimal TATA promoter and empty vector. *, p

    Techniques Used: Sequencing, Transfection, Luciferase, Activity Assay, Activation Assay, Plasmid Preparation

    2) Product Images from "A Pan-European Study of the C9orf72 Repeat Associated with FTLD: Geographic Prevalence, Genomic Instability, and Intermediate Repeats"

    Article Title: A Pan-European Study of the C9orf72 Repeat Associated with FTLD: Geographic Prevalence, Genomic Instability, and Intermediate Repeats

    Journal: Human Mutation

    doi: 10.1002/humu.22244

    Transcriptional activity of C9orf72 promoter with alleles of different repeat length. Bars represent relative Gaussia / Cypridina luciferase activities (RLA) for the different C9orf72 constructs compared with the wild-type allele of 2 units, for an increasing amount of repeat units. Values represent the mean (±SDEV) of 36 independent measurements relative to the 2 units wild-type allele. The significance of differences in expression was calculated using the Mann–Whitney U test. P values are presented above the bars.
    Figure Legend Snippet: Transcriptional activity of C9orf72 promoter with alleles of different repeat length. Bars represent relative Gaussia / Cypridina luciferase activities (RLA) for the different C9orf72 constructs compared with the wild-type allele of 2 units, for an increasing amount of repeat units. Values represent the mean (±SDEV) of 36 independent measurements relative to the 2 units wild-type allele. The significance of differences in expression was calculated using the Mann–Whitney U test. P values are presented above the bars.

    Techniques Used: Activity Assay, Luciferase, Construct, Expressing, MANN-WHITNEY

    Related Articles

    Transfection:

    Article Title: Sequence Variability in Viral Genome Non-coding Regions Likely Contribute to Observed Differences in Viral Replication Amongst MARV Strains
    Article Snippet: .. 72 h post-RNA transfection, cellular supernatants were removed and used to measure levels of GLuc and CLuc protein signals using the BioLux Cypridina and Gaussia Assay Kits (NEB, Ipswich, MA) according to the manufacturer’s recommendations. .. Luciferase reporter assays with MARV-Mus were conducted by infecting HeLa cells at MOI 0.1 then transfecting minigenome RNA as described above; reporter gene levels were measured 48-72 h post-transfection.

    Luciferase:

    Article Title: Comprehensive Interrogation of Natural TALE DNA Binding Modules and Transcriptional Repressor Domains
    Article Snippet: .. Dual luciferase reporter assays were carried out with the BioLux Gaussia luciferase flex assay kit and BioLux Cypridina luciferase assay kit (New England Biolabs) following the manufacturer’s recommended protocol. .. For each sample, 20 uL of the media were added into a 96-well assay plate, mixed with each one of the dual luciferase assay mixes.

    Article Title: HPV16 and 18 genome amplification show different E4-dependence, with 16E4 enhancing E1 nuclear accumulation and replicative efficiency via its cell cycle arrest and kinase activation functions
    Article Snippet: .. 24 hours later, culture media was collected and luciferase activity measured using a BioLux Gaussia Luciferase Assay Kit and a BioLux Cypridina Luciferase Assay Kit (NEB, UK). .. Gaussia Luciferase activity was normalized against Cypridina Luciferase activity.

    Article Title: Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
    Article Snippet: .. GluN1 promoter activities were followed with BioLux Cypridina and Gaussia luciferase assay kits (New England Biolabs, E3309S and E3300S). .. Wnt5a conditioned media and L-cell conditioned media were harvested from Wnt5a producing cells (ATCC CRL-2814) and L cells (ATCC CRL-2648).

    Article Title: ChREBP Regulates Itself and Metabolic Genes Implicated in Lipid Accumulation in β–Cell Line
    Article Snippet: .. We then sequentially detected secreted luciferases using Gaussia luciferase and Cypridina luciferase assay kits (New England Biolabs). .. Luciferase expressed from Gaussia luciferase reporter plasmid is normalized by the activity of Cypridina luciferase.

    Article Title: A Pan-European Study of the C9orf72 Repeat Associated with FTLD: Geographic Prevalence, Genomic Instability, and Intermediate Repeats
    Article Snippet: .. After 24 hr, Gaussia luciferase activities (LAG ) and Cypridina luciferase activities (LAC ) were measured in duplo in the growth medium using a BioLux Gaussia and Cypridina Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA) and a Veritas Microplate Luminometer with Dual Reagent Injectors Luminometer (Promega, Madison, WI, USA). .. To correct for transfection efficiency and DNA uptake, the relative luciferase activity (RLA) was calculated as RLA = LAG /LAC .

    Article Title: Promoter DNA methylation contributes to human β-defensin-1 deficiency in atopic dermatitis
    Article Snippet: .. In each experiment, pCMV-CLuc 2 Control Plasmid (New England Biolabs), encoding the secreted Cypridina luciferase (CLuc), was co-transfected as a normalization control and luminescence was then determined from the supernatant using the BioLux Cypridina Luciferase Assay Kit (New England Biolabs). .. Reporter activity was normalized by calculating the ratio of Lucia to CLuc values.

    Article Title: HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties
    Article Snippet: .. Cypridina and Gaussia-Dura luciferase activities were measured in conditioned media 48 hr post-transfection with the BioLux Cypridina Luciferase and BioLux Gaussia Luciferase Assay Kits (New England Biolabs), respectively. .. Chromatin immunoprecipitation sequencing (ChIP-seq) A confluent 15 cm culture plate of cells was used per immunoprecipitation.

    Activity Assay:

    Article Title: HPV16 and 18 genome amplification show different E4-dependence, with 16E4 enhancing E1 nuclear accumulation and replicative efficiency via its cell cycle arrest and kinase activation functions
    Article Snippet: .. 24 hours later, culture media was collected and luciferase activity measured using a BioLux Gaussia Luciferase Assay Kit and a BioLux Cypridina Luciferase Assay Kit (NEB, UK). .. Gaussia Luciferase activity was normalized against Cypridina Luciferase activity.

    Plasmid Preparation:

    Article Title: Promoter DNA methylation contributes to human β-defensin-1 deficiency in atopic dermatitis
    Article Snippet: .. In each experiment, pCMV-CLuc 2 Control Plasmid (New England Biolabs), encoding the secreted Cypridina luciferase (CLuc), was co-transfected as a normalization control and luminescence was then determined from the supernatant using the BioLux Cypridina Luciferase Assay Kit (New England Biolabs). .. Reporter activity was normalized by calculating the ratio of Lucia to CLuc values.

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  • 98
    New England Biolabs cypridina luciferase assay kits
    Effect of caChREBP on Gpd1 or Rgs16 ChoRE-containing promoters. (A) Alignment of ChoRE sequence presented on Gpd1 promoters among rat (at the position -1943 to -1927), mouse, cow, dog, rhesus, chimpanzee, and human. Sequence assemblies and coordinates are as follows: Rat: Mar.2012 ChrX(+): 115873245–11587361, Mouse: Dec.2011 Chr15(+): 99716109–99716125, Cow: Oct.2011 Chr5(-): 32732081–32732065, Dog: Sep.2011 Chr27(-): 4623314–4623298, Rhesus: Oct.2010 Chr11(+): 47359512–47359528, Chimpanzee: Feb.2011 Chr12(-): 39238594–39238578, and Human: Feb.2009 Chr12(+): 50496575–50496591. Color coding: light grey, identical residues; dark grey, unconserved residues. (B) caChREBP cannot activate Gpd1 ChoRE-containing promoter. We co-transfected luciferase reporter driven by two copies of rat Gpd1 ChoRE (position -1943 to -1927 relative to TSS) upstream of minimal TATA promoter with caChREBP in 832/13 cells in RPMI with 5.5 mmol/l D-glucose. Gaussia luciferase activity was measured at 48h and normalized to <t>Cypridina</t> luciferase, and expressed as fold activation over the activity seen in cells transfected with two copies of ChoRE with minimal TATA promoter and empty vector. *, p
    Cypridina Luciferase Assay Kits, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cypridina luciferase assay kits/product/New England Biolabs
    Average 98 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    cypridina luciferase assay kits - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results


    Effect of caChREBP on Gpd1 or Rgs16 ChoRE-containing promoters. (A) Alignment of ChoRE sequence presented on Gpd1 promoters among rat (at the position -1943 to -1927), mouse, cow, dog, rhesus, chimpanzee, and human. Sequence assemblies and coordinates are as follows: Rat: Mar.2012 ChrX(+): 115873245–11587361, Mouse: Dec.2011 Chr15(+): 99716109–99716125, Cow: Oct.2011 Chr5(-): 32732081–32732065, Dog: Sep.2011 Chr27(-): 4623314–4623298, Rhesus: Oct.2010 Chr11(+): 47359512–47359528, Chimpanzee: Feb.2011 Chr12(-): 39238594–39238578, and Human: Feb.2009 Chr12(+): 50496575–50496591. Color coding: light grey, identical residues; dark grey, unconserved residues. (B) caChREBP cannot activate Gpd1 ChoRE-containing promoter. We co-transfected luciferase reporter driven by two copies of rat Gpd1 ChoRE (position -1943 to -1927 relative to TSS) upstream of minimal TATA promoter with caChREBP in 832/13 cells in RPMI with 5.5 mmol/l D-glucose. Gaussia luciferase activity was measured at 48h and normalized to Cypridina luciferase, and expressed as fold activation over the activity seen in cells transfected with two copies of ChoRE with minimal TATA promoter and empty vector. *, p

    Journal: PLoS ONE

    Article Title: ChREBP Regulates Itself and Metabolic Genes Implicated in Lipid Accumulation in β–Cell Line

    doi: 10.1371/journal.pone.0147411

    Figure Lengend Snippet: Effect of caChREBP on Gpd1 or Rgs16 ChoRE-containing promoters. (A) Alignment of ChoRE sequence presented on Gpd1 promoters among rat (at the position -1943 to -1927), mouse, cow, dog, rhesus, chimpanzee, and human. Sequence assemblies and coordinates are as follows: Rat: Mar.2012 ChrX(+): 115873245–11587361, Mouse: Dec.2011 Chr15(+): 99716109–99716125, Cow: Oct.2011 Chr5(-): 32732081–32732065, Dog: Sep.2011 Chr27(-): 4623314–4623298, Rhesus: Oct.2010 Chr11(+): 47359512–47359528, Chimpanzee: Feb.2011 Chr12(-): 39238594–39238578, and Human: Feb.2009 Chr12(+): 50496575–50496591. Color coding: light grey, identical residues; dark grey, unconserved residues. (B) caChREBP cannot activate Gpd1 ChoRE-containing promoter. We co-transfected luciferase reporter driven by two copies of rat Gpd1 ChoRE (position -1943 to -1927 relative to TSS) upstream of minimal TATA promoter with caChREBP in 832/13 cells in RPMI with 5.5 mmol/l D-glucose. Gaussia luciferase activity was measured at 48h and normalized to Cypridina luciferase, and expressed as fold activation over the activity seen in cells transfected with two copies of ChoRE with minimal TATA promoter and empty vector. *, p

    Article Snippet: We then sequentially detected secreted luciferases using Gaussia luciferase and Cypridina luciferase assay kits (New England Biolabs).

    Techniques: Sequencing, Transfection, Luciferase, Activity Assay, Activation Assay, Plasmid Preparation

    Transcriptional activity of C9orf72 promoter with alleles of different repeat length. Bars represent relative Gaussia / Cypridina luciferase activities (RLA) for the different C9orf72 constructs compared with the wild-type allele of 2 units, for an increasing amount of repeat units. Values represent the mean (±SDEV) of 36 independent measurements relative to the 2 units wild-type allele. The significance of differences in expression was calculated using the Mann–Whitney U test. P values are presented above the bars.

    Journal: Human Mutation

    Article Title: A Pan-European Study of the C9orf72 Repeat Associated with FTLD: Geographic Prevalence, Genomic Instability, and Intermediate Repeats

    doi: 10.1002/humu.22244

    Figure Lengend Snippet: Transcriptional activity of C9orf72 promoter with alleles of different repeat length. Bars represent relative Gaussia / Cypridina luciferase activities (RLA) for the different C9orf72 constructs compared with the wild-type allele of 2 units, for an increasing amount of repeat units. Values represent the mean (±SDEV) of 36 independent measurements relative to the 2 units wild-type allele. The significance of differences in expression was calculated using the Mann–Whitney U test. P values are presented above the bars.

    Article Snippet: After 24 hr, Gaussia luciferase activities (LAG ) and Cypridina luciferase activities (LAC ) were measured in duplo in the growth medium using a BioLux Gaussia and Cypridina Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA) and a Veritas Microplate Luminometer with Dual Reagent Injectors Luminometer (Promega, Madison, WI, USA).

    Techniques: Activity Assay, Luciferase, Construct, Expressing, MANN-WHITNEY