luna cell ready lysis module  (New England Biolabs)


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    Name:
    Luna Cell Ready Lysis Module
    Description:
    The Luna Cell Ready Lysis Module coordinates cell lysis RNA release and genomic DNA removal simultaneously resulting in cell lysate ready for direct RT qPCR analysis with Luna Universal One Step RT qPCR kits
    Catalog Number:
    E3032S
    Price:
    495
    Category:
    RT PCR Kits
    Size:
    100 rxns
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    Structured Review

    New England Biolabs luna cell ready lysis module
    Luna Cell Ready Lysis Module
    The Luna Cell Ready Lysis Module coordinates cell lysis RNA release and genomic DNA removal simultaneously resulting in cell lysate ready for direct RT qPCR analysis with Luna Universal One Step RT qPCR kits
    https://www.bioz.com/result/luna cell ready lysis module/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna cell ready lysis module - by Bioz Stars, 2021-05
    94/100 stars

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    Related Articles

    Lysis:

    Article Title: Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms
    Article Snippet: Knockdown validation with RT-qPCR in A549-ACE2 cells Gene-specific qPCR primers targeting all genes represented in the OnTargetPlus library were purchased and arrayed in a 96-well format identical to that of the siRNA library (IDT; table S13). .. A549-ACE2 cells treated with siRNA were lysed using the Luna Cell Ready Lysis Module (New England Biolabs) following the manufacturer’s protocol. .. The lysate was used directly for gene quantification by RT-qPCR with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs), using the gene-specific PCR primers and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene.

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: 25 μl Luna Cell Ready Lysis Buffer (2X), 5 μl of RNase-free DNase I (10x), 2 μl of Luna Cell ready RNA Protection Reagent (25x), 2 μl of Luna Cell Ready Protease (25x) and 6 μl of Nuclease-free water for total volume of 40 μl / well were added and incubated at 37°C for 10 min. .. The cell lysis reactions were terminated by adding 5 μl of Luna Cell Ready Stop Solution to each well and mixed well then incubated at 25°C for 5 min. 1.5 μl of the above cell lysis mix were used to program q-PCR reactions using Luna Universal One-Step RT-qPCR kit (NEB # E 3006). ..

    Article Title: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
    Article Snippet: .. Synthetic RNAs were spiked into Hela cells, which were then diluted and lysed using Luna® Cell Ready Lysis Module (E3032). .. Each lysate was then diluted 10x with 0.1x TE +0.01% Tween 20 and 1 μL was added to standard colorimetric LAMP reactions.

    Incubation:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: 25 μl Luna Cell Ready Lysis Buffer (2X), 5 μl of RNase-free DNase I (10x), 2 μl of Luna Cell ready RNA Protection Reagent (25x), 2 μl of Luna Cell Ready Protease (25x) and 6 μl of Nuclease-free water for total volume of 40 μl / well were added and incubated at 37°C for 10 min. .. The cell lysis reactions were terminated by adding 5 μl of Luna Cell Ready Stop Solution to each well and mixed well then incubated at 25°C for 5 min. 1.5 μl of the above cell lysis mix were used to program q-PCR reactions using Luna Universal One-Step RT-qPCR kit (NEB # E 3006). ..

    Quantitative RT-PCR:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: 25 μl Luna Cell Ready Lysis Buffer (2X), 5 μl of RNase-free DNase I (10x), 2 μl of Luna Cell ready RNA Protection Reagent (25x), 2 μl of Luna Cell Ready Protease (25x) and 6 μl of Nuclease-free water for total volume of 40 μl / well were added and incubated at 37°C for 10 min. .. The cell lysis reactions were terminated by adding 5 μl of Luna Cell Ready Stop Solution to each well and mixed well then incubated at 25°C for 5 min. 1.5 μl of the above cell lysis mix were used to program q-PCR reactions using Luna Universal One-Step RT-qPCR kit (NEB # E 3006). ..

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  • 94
    New England Biolabs luna cell ready lysis module
    Luna Cell Ready Lysis Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna cell ready lysis module/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna cell ready lysis module - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

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