luna cell ready probe one step rt qpcr kit  (New England Biolabs)


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    Luna Cell Ready Probe One Step RT qPCR Kit
    Description:
    The Luna Cell Ready Probe One Step RT qPCR Kit provides all the necessary components for direct probe based RNA detection and quantitation from cell lysate bypassing the need for RNA extraction and purification The Luna Cell Ready Probe One Step RT qPCR Kit is composed of 1 the Luna Cell Ready Lysis Module NEB E3032S and 2 the Luna Universal Probe One Step RT qPCR Kit NEB E3006L
    Catalog Number:
    E3031S
    Price:
    920
    Category:
    RT PCR Kits
    Size:
    100 rxns
    Buy from Supplier


    Structured Review

    New England Biolabs luna cell ready probe one step rt qpcr kit
    Luna Cell Ready Probe One Step RT qPCR Kit
    The Luna Cell Ready Probe One Step RT qPCR Kit provides all the necessary components for direct probe based RNA detection and quantitation from cell lysate bypassing the need for RNA extraction and purification The Luna Cell Ready Probe One Step RT qPCR Kit is composed of 1 the Luna Cell Ready Lysis Module NEB E3032S and 2 the Luna Universal Probe One Step RT qPCR Kit NEB E3006L
    https://www.bioz.com/result/luna cell ready probe one step rt qpcr kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna cell ready probe one step rt qpcr kit - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Dissecting transcriptional amplification by MYC"

    Article Title: Dissecting transcriptional amplification by MYC

    Journal: eLife

    doi: 10.7554/eLife.52483

    MYC-Box mutations change both non-E and E-box promoter-output at the RNA level. ( A and B ) Cells were transfected as noted with 80 ng of empty vector, MYC, MBII or MBIII expressing vectors, 100 ng of non-E-box or E-box reporter and 2 ng of GR plasmids . Luciferase and renilla luciferase were assayed. ( C ). Transfected cells as in B were harvested and RNA extracted for RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Experiments were performed in biological triplicate. Standard deviations are indicated. *,** and *** indicate p≤0.05, 0.01, 0.001, respectively, one-tailed t-test. Experiments performed in triplicate (A and B, n = 3; C, n = 1).
    Figure Legend Snippet: MYC-Box mutations change both non-E and E-box promoter-output at the RNA level. ( A and B ) Cells were transfected as noted with 80 ng of empty vector, MYC, MBII or MBIII expressing vectors, 100 ng of non-E-box or E-box reporter and 2 ng of GR plasmids . Luciferase and renilla luciferase were assayed. ( C ). Transfected cells as in B were harvested and RNA extracted for RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Experiments were performed in biological triplicate. Standard deviations are indicated. *,** and *** indicate p≤0.05, 0.01, 0.001, respectively, one-tailed t-test. Experiments performed in triplicate (A and B, n = 3; C, n = 1).

    Techniques Used: Transfection, Plasmid Preparation, Expressing, Luciferase, Quantitative RT-PCR, One-tailed Test

    MYC-EGFP binding at chromosomally integrated-reporters parallels activity and emulates native promoters. Top: diagram of integrated-lentivirus CFP reporters. ( A ) Left-ChIP-PCR to assay MYC-EGFP binding in the absence of activator of uninduced (lanes 2 and 6) versus doxycycline-induced (lanes 4 and 8) MYC-EGFP, at non-E-box (lanes 2 and 4) versus E-box-bearing (lanes 6 and 8) lentivirus-integrated reporter promoters. Right-ChIP-PCR to assay MYC-EGFP binding in the presence of GAL4/VP16 of uninduced (lanes 10 and 14) versus doxycycline-induced (lanes 12 and 16) MYC-EGFP at non-E-box (lanes 10 and 12) versus E-box bearing (lanes 14 and 16) lentivirus integrated reporter-promoters. ( B ) EZH2 RNA levels from cells expressing non-induced or doxycycline induced MYC-EGFP(WT) or MYC-EGFP-mutants MBI, MBII, MBIII or MBIV and assayed by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ( C ) Binding of uninduced and induced MYC-EGFP to endogenous promoters. The asterisk (*) indicates that binding to BEX in heterochromatin was so low as to be virtually unexpressed as previously reported ( Nie et al., 2012 ). ( D ). Uninduced or doxycycline-induced MYC-EGFP(WT) or MYC-EGFP-mutants (MBI, MBII, MBIII, or MBIV) binding to the native EZH2 promoter assayed by ChIP-PCR. The mean and SD for representative experiments performed in triplicate are shown, n ≥ 2.
    Figure Legend Snippet: MYC-EGFP binding at chromosomally integrated-reporters parallels activity and emulates native promoters. Top: diagram of integrated-lentivirus CFP reporters. ( A ) Left-ChIP-PCR to assay MYC-EGFP binding in the absence of activator of uninduced (lanes 2 and 6) versus doxycycline-induced (lanes 4 and 8) MYC-EGFP, at non-E-box (lanes 2 and 4) versus E-box-bearing (lanes 6 and 8) lentivirus-integrated reporter promoters. Right-ChIP-PCR to assay MYC-EGFP binding in the presence of GAL4/VP16 of uninduced (lanes 10 and 14) versus doxycycline-induced (lanes 12 and 16) MYC-EGFP at non-E-box (lanes 10 and 12) versus E-box bearing (lanes 14 and 16) lentivirus integrated reporter-promoters. ( B ) EZH2 RNA levels from cells expressing non-induced or doxycycline induced MYC-EGFP(WT) or MYC-EGFP-mutants MBI, MBII, MBIII or MBIV and assayed by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ( C ) Binding of uninduced and induced MYC-EGFP to endogenous promoters. The asterisk (*) indicates that binding to BEX in heterochromatin was so low as to be virtually unexpressed as previously reported ( Nie et al., 2012 ). ( D ). Uninduced or doxycycline-induced MYC-EGFP(WT) or MYC-EGFP-mutants (MBI, MBII, MBIII, or MBIV) binding to the native EZH2 promoter assayed by ChIP-PCR. The mean and SD for representative experiments performed in triplicate are shown, n ≥ 2.

    Techniques Used: Binding Assay, Activity Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Expressing, Quantitative RT-PCR

    Related Articles

    Plasmid Preparation:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: RNA was measure using Nanodrop. .. For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions. ..

    Mutagenesis:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: RNA was measure using Nanodrop. .. For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions. ..

    Stable Transfection:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: RNA was measure using Nanodrop. .. For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions. ..

    Quantitative RT-PCR:

    Article Title: Dissecting transcriptional amplification by MYC
    Article Snippet: RNA was measure using Nanodrop. .. For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions. ..

    Article Title: LY-CoV1404 potently neutralizes SARS-CoV-2 variants
    Article Snippet: Infectious titers were determined for a subset of virus preparations by infection of VeroE6 cells (ATCC CRL-1586) with serially diluted virus followed by staining with an anti-luciferase antibody (Novus Cat # NB600-307PEATT594) and analysis by fluorescence-activated cell sorting using a Becton Dickinson LSRFortessaTM X-20. .. Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system. .. Relative luciferase reporter signal read-out was determined by luciferase assay (Promega Cat # E2650) of extracts from VeroE6 cells infected with serially diluted virus.

    Isolation:

    Article Title: LY-CoV1404 potently neutralizes SARS-CoV-2 variants
    Article Snippet: Infectious titers were determined for a subset of virus preparations by infection of VeroE6 cells (ATCC CRL-1586) with serially diluted virus followed by staining with an anti-luciferase antibody (Novus Cat # NB600-307PEATT594) and analysis by fluorescence-activated cell sorting using a Becton Dickinson LSRFortessaTM X-20. .. Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system. .. Relative luciferase reporter signal read-out was determined by luciferase assay (Promega Cat # E2650) of extracts from VeroE6 cells infected with serially diluted virus.

    Quantitation Assay:

    Article Title: LY-CoV1404 potently neutralizes SARS-CoV-2 variants
    Article Snippet: Infectious titers were determined for a subset of virus preparations by infection of VeroE6 cells (ATCC CRL-1586) with serially diluted virus followed by staining with an anti-luciferase antibody (Novus Cat # NB600-307PEATT594) and analysis by fluorescence-activated cell sorting using a Becton Dickinson LSRFortessaTM X-20. .. Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system. .. Relative luciferase reporter signal read-out was determined by luciferase assay (Promega Cat # E2650) of extracts from VeroE6 cells infected with serially diluted virus.

    Real-time Polymerase Chain Reaction:

    Article Title: LY-CoV1404 potently neutralizes SARS-CoV-2 variants
    Article Snippet: Infectious titers were determined for a subset of virus preparations by infection of VeroE6 cells (ATCC CRL-1586) with serially diluted virus followed by staining with an anti-luciferase antibody (Novus Cat # NB600-307PEATT594) and analysis by fluorescence-activated cell sorting using a Becton Dickinson LSRFortessaTM X-20. .. Particle titers were determined by isolation of genomic RNA and quantitation by RT-qPCR (NEB # E3031) using an Applied Biosystems vii7A™ Real-Time PCR system. .. Relative luciferase reporter signal read-out was determined by luciferase assay (Promega Cat # E2650) of extracts from VeroE6 cells infected with serially diluted virus.

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    New England Biolabs luna cell ready probe one step rt qpcr kit
    <t>MYC-Box</t> mutations change both non-E and E-box promoter-output at the RNA level. ( A and B ) Cells were transfected as noted with 80 ng of empty vector, MYC, MBII or MBIII expressing vectors, 100 ng of non-E-box or E-box reporter and 2 ng of GR plasmids . Luciferase and renilla luciferase were assayed. ( C ). Transfected cells as in B were harvested and RNA extracted for <t>RT-qPCR</t> using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Experiments were performed in biological triplicate. Standard deviations are indicated. *,** and *** indicate p≤0.05, 0.01, 0.001, respectively, one-tailed t-test. Experiments performed in triplicate (A and B, n = 3; C, n = 1).
    Luna Cell Ready Probe One Step Rt Qpcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna cell ready probe one step rt qpcr kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna cell ready probe one step rt qpcr kit - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    MYC-Box mutations change both non-E and E-box promoter-output at the RNA level. ( A and B ) Cells were transfected as noted with 80 ng of empty vector, MYC, MBII or MBIII expressing vectors, 100 ng of non-E-box or E-box reporter and 2 ng of GR plasmids . Luciferase and renilla luciferase were assayed. ( C ). Transfected cells as in B were harvested and RNA extracted for RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Experiments were performed in biological triplicate. Standard deviations are indicated. *,** and *** indicate p≤0.05, 0.01, 0.001, respectively, one-tailed t-test. Experiments performed in triplicate (A and B, n = 3; C, n = 1).

    Journal: eLife

    Article Title: Dissecting transcriptional amplification by MYC

    doi: 10.7554/eLife.52483

    Figure Lengend Snippet: MYC-Box mutations change both non-E and E-box promoter-output at the RNA level. ( A and B ) Cells were transfected as noted with 80 ng of empty vector, MYC, MBII or MBIII expressing vectors, 100 ng of non-E-box or E-box reporter and 2 ng of GR plasmids . Luciferase and renilla luciferase were assayed. ( C ). Transfected cells as in B were harvested and RNA extracted for RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Experiments were performed in biological triplicate. Standard deviations are indicated. *,** and *** indicate p≤0.05, 0.01, 0.001, respectively, one-tailed t-test. Experiments performed in triplicate (A and B, n = 3; C, n = 1).

    Article Snippet: For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions.

    Techniques: Transfection, Plasmid Preparation, Expressing, Luciferase, Quantitative RT-PCR, One-tailed Test

    MYC-EGFP binding at chromosomally integrated-reporters parallels activity and emulates native promoters. Top: diagram of integrated-lentivirus CFP reporters. ( A ) Left-ChIP-PCR to assay MYC-EGFP binding in the absence of activator of uninduced (lanes 2 and 6) versus doxycycline-induced (lanes 4 and 8) MYC-EGFP, at non-E-box (lanes 2 and 4) versus E-box-bearing (lanes 6 and 8) lentivirus-integrated reporter promoters. Right-ChIP-PCR to assay MYC-EGFP binding in the presence of GAL4/VP16 of uninduced (lanes 10 and 14) versus doxycycline-induced (lanes 12 and 16) MYC-EGFP at non-E-box (lanes 10 and 12) versus E-box bearing (lanes 14 and 16) lentivirus integrated reporter-promoters. ( B ) EZH2 RNA levels from cells expressing non-induced or doxycycline induced MYC-EGFP(WT) or MYC-EGFP-mutants MBI, MBII, MBIII or MBIV and assayed by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ( C ) Binding of uninduced and induced MYC-EGFP to endogenous promoters. The asterisk (*) indicates that binding to BEX in heterochromatin was so low as to be virtually unexpressed as previously reported ( Nie et al., 2012 ). ( D ). Uninduced or doxycycline-induced MYC-EGFP(WT) or MYC-EGFP-mutants (MBI, MBII, MBIII, or MBIV) binding to the native EZH2 promoter assayed by ChIP-PCR. The mean and SD for representative experiments performed in triplicate are shown, n ≥ 2.

    Journal: eLife

    Article Title: Dissecting transcriptional amplification by MYC

    doi: 10.7554/eLife.52483

    Figure Lengend Snippet: MYC-EGFP binding at chromosomally integrated-reporters parallels activity and emulates native promoters. Top: diagram of integrated-lentivirus CFP reporters. ( A ) Left-ChIP-PCR to assay MYC-EGFP binding in the absence of activator of uninduced (lanes 2 and 6) versus doxycycline-induced (lanes 4 and 8) MYC-EGFP, at non-E-box (lanes 2 and 4) versus E-box-bearing (lanes 6 and 8) lentivirus-integrated reporter promoters. Right-ChIP-PCR to assay MYC-EGFP binding in the presence of GAL4/VP16 of uninduced (lanes 10 and 14) versus doxycycline-induced (lanes 12 and 16) MYC-EGFP at non-E-box (lanes 10 and 12) versus E-box bearing (lanes 14 and 16) lentivirus integrated reporter-promoters. ( B ) EZH2 RNA levels from cells expressing non-induced or doxycycline induced MYC-EGFP(WT) or MYC-EGFP-mutants MBI, MBII, MBIII or MBIV and assayed by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ( C ) Binding of uninduced and induced MYC-EGFP to endogenous promoters. The asterisk (*) indicates that binding to BEX in heterochromatin was so low as to be virtually unexpressed as previously reported ( Nie et al., 2012 ). ( D ). Uninduced or doxycycline-induced MYC-EGFP(WT) or MYC-EGFP-mutants (MBI, MBII, MBIII, or MBIV) binding to the native EZH2 promoter assayed by ChIP-PCR. The mean and SD for representative experiments performed in triplicate are shown, n ≥ 2.

    Article Snippet: For measurement of human EZH2 RNA in pTripZ-EGFP, or pTripZ-lentivirus vector directed wild-type MYC-EGFP and mutant-MBI, II, III or IV-MYC-EGFP U2OS stable cell lines, RNA was prepared using Luna Cell Ready One-Step RT-qPCR Kit (NEB # E3031) following the manufacturer’s instructions.

    Techniques: Binding Assay, Activity Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Expressing, Quantitative RT-PCR