luna universal one step rt qpcr kit  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    Luna Universal One Step RT qPCR Kit
    Description:
    Luna Universal One Step RT qPCR Kit 2 500 rxns
    Catalog Number:
    e3005e
    Price:
    2074
    Size:
    2 500 rxns
    Category:
    RT PCR Kits
    Buy from Supplier


    Structured Review

    New England Biolabs luna universal one step rt qpcr kit
    Luna Universal One Step RT qPCR Kit
    Luna Universal One Step RT qPCR Kit 2 500 rxns
    https://www.bioz.com/result/luna universal one step rt qpcr kit/product/New England Biolabs
    Average 98 stars, based on 160 article reviews
    Price from $9.99 to $1999.99
    luna universal one step rt qpcr kit - by Bioz Stars, 2020-05
    98/100 stars

    Images

    1) Product Images from "LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts"

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts

    Journal: Journal of Orthopaedic Surgery and Research

    doi: 10.1186/s13018-019-1430-4

    Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p
    Figure Legend Snippet: Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Techniques Used: Quantitative RT-PCR

    2) Product Images from "Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction"

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    Journal: Medicine

    doi: 10.1097/MD.0000000000013066

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.
    Figure Legend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    3) Product Images from "Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing"

    Article Title: Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2019.00976

    Effects of external RNA pesticide on inhibiting S. sclerotiorum from causing lesions on canola leaves comparing dsRNA targeting agl -2 at 800 ng/20 μl, 400 ng/20 μl, 200 ng/20 μl, dsRNA targeting agl -4 at 800 ng/20 μl as control (from left to right), confirmed by (A) RT-qPCR with reduced expression levels of agl -2 at 200 and 400 ng, and lesion comparison at (B) 36, (C) 48, and (D) 72 h post-inoculation.
    Figure Legend Snippet: Effects of external RNA pesticide on inhibiting S. sclerotiorum from causing lesions on canola leaves comparing dsRNA targeting agl -2 at 800 ng/20 μl, 400 ng/20 μl, 200 ng/20 μl, dsRNA targeting agl -4 at 800 ng/20 μl as control (from left to right), confirmed by (A) RT-qPCR with reduced expression levels of agl -2 at 200 and 400 ng, and lesion comparison at (B) 36, (C) 48, and (D) 72 h post-inoculation.

    Techniques Used: Quantitative RT-PCR, Expressing

    4) Product Images from "Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans"

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-27813-3

    Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.
    Figure Legend Snippet: Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Techniques Used: Expressing, Quantitative RT-PCR

    5) Product Images from "Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization"

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006857

    Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: RNA Expression, Transfection, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Fluorescence

    Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: Expressing, Capillary Electrochromatography, Transfection, Mutagenesis, BAC Assay, Clone Assay, Real-time Polymerase Chain Reaction

    6) Product Images from "Globoside Is Dispensable for Parvovirus B19 Entry but Essential at a Postentry Step for Productive Infection"

    Article Title: Globoside Is Dispensable for Parvovirus B19 Entry but Essential at a Postentry Step for Productive Infection

    Journal: Journal of Virology

    doi: 10.1128/JVI.00972-19

    B3GalNT1 KO UT7/Epo cells lack B3GalNT1 transcripts, do not express Gb4, and proliferate normally. (A) Detection of B3GalNT1 mRNA. Total mRNA was isolated from WT cells and from two single cell-derived RFP-expressing clones (KO1 and KO2) and used to detect B3GalNT1 transcripts by RT-qPCR. The amplicons were used in a nested PCR to ensure sufficient sensitivity. Dilutions (1% and 10%) of the WT amplicons were loaded as a reference. GAPDH mRNA was used as a loading control. (B) Detection of Gb4 by immunofluorescence. WT and KO cells were stained with anti-Gb4 antibody, fixed, and visualized by confocal microscopy. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). (C) Phase-contrast images of WT and KO cells showing no morphological differences. (D) Cell proliferation of WT and KO cells. Cells were incubated at 37°C and counted at the indicated days.
    Figure Legend Snippet: B3GalNT1 KO UT7/Epo cells lack B3GalNT1 transcripts, do not express Gb4, and proliferate normally. (A) Detection of B3GalNT1 mRNA. Total mRNA was isolated from WT cells and from two single cell-derived RFP-expressing clones (KO1 and KO2) and used to detect B3GalNT1 transcripts by RT-qPCR. The amplicons were used in a nested PCR to ensure sufficient sensitivity. Dilutions (1% and 10%) of the WT amplicons were loaded as a reference. GAPDH mRNA was used as a loading control. (B) Detection of Gb4 by immunofluorescence. WT and KO cells were stained with anti-Gb4 antibody, fixed, and visualized by confocal microscopy. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). (C) Phase-contrast images of WT and KO cells showing no morphological differences. (D) Cell proliferation of WT and KO cells. Cells were incubated at 37°C and counted at the indicated days.

    Techniques Used: Isolation, Derivative Assay, Expressing, Clone Assay, Quantitative RT-PCR, Nested PCR, Immunofluorescence, Staining, Confocal Microscopy, Incubation

    Gb4 is dispensable for B19V cell attachment, internalization, and VP1u exposure. (A) Detection of B19V attachment by immunofluorescence. B19V was incubated with cells at 4°C for 1 h, followed by four washes with cold PBS. Cells were fixed, stained with antibody 860-55D against capsids, and visualized by confocal microscopy. (B) Detection of B19V internalization by immunofluorescence. B19V was incubated with cells at 37°C for 1 h, washed four times with PBS, and trypsinized to remove noninternalized viruses. Cells were fixed, stained with antibody 860-55D, and visualized by confocal microscopy. (C) Quantification of B19V attachment. B19V was incubated with cells at 4°C for 1 h, followed by four washes with cold PBS. The number of virions bound to the cells was quantified by PCR. (D) Quantification of B19V internalization. B19V was incubated with cells at 37°C for 1 h, washed four times with PBS, trypsinized to remove noninternalized viruses, and quantified by PCR. WT cells incubated at 4°C serve as negative controls (no internalization). (E) Quantification of VP1u exposure from free virus or bound to cells. Virions were immunoprecipitated with antibody 860-55D against capsids (total capsids) and a rabbit antibody against the PLA 2 region (α-VP1u), followed by qPCR. Normal rabbit IgG was used as a negative control. P values were calculated according to Student’s t test. *, P
    Figure Legend Snippet: Gb4 is dispensable for B19V cell attachment, internalization, and VP1u exposure. (A) Detection of B19V attachment by immunofluorescence. B19V was incubated with cells at 4°C for 1 h, followed by four washes with cold PBS. Cells were fixed, stained with antibody 860-55D against capsids, and visualized by confocal microscopy. (B) Detection of B19V internalization by immunofluorescence. B19V was incubated with cells at 37°C for 1 h, washed four times with PBS, and trypsinized to remove noninternalized viruses. Cells were fixed, stained with antibody 860-55D, and visualized by confocal microscopy. (C) Quantification of B19V attachment. B19V was incubated with cells at 4°C for 1 h, followed by four washes with cold PBS. The number of virions bound to the cells was quantified by PCR. (D) Quantification of B19V internalization. B19V was incubated with cells at 37°C for 1 h, washed four times with PBS, trypsinized to remove noninternalized viruses, and quantified by PCR. WT cells incubated at 4°C serve as negative controls (no internalization). (E) Quantification of VP1u exposure from free virus or bound to cells. Virions were immunoprecipitated with antibody 860-55D against capsids (total capsids) and a rabbit antibody against the PLA 2 region (α-VP1u), followed by qPCR. Normal rabbit IgG was used as a negative control. P values were calculated according to Student’s t test. *, P

    Techniques Used: Cell Attachment Assay, Immunofluorescence, Incubation, Staining, Confocal Microscopy, Polymerase Chain Reaction, Immunoprecipitation, Proximity Ligation Assay, Real-time Polymerase Chain Reaction, Negative Control

    7) Product Images from "Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation"

    Article Title: Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation

    Journal: eLife

    doi: 10.7554/eLife.34338

    GluRIIA knock down phenocopies GluRIIA mutants. ( A ) Representative images of NMJs on muscle six immunostained with antibodies that recognize GluRIIA, GluRIIC, and GluRIID receptor subunits in wild type ( w 1118 ), GluRIIA mutants ( w 1118 ;GluRIIA SP16 ), and G14 > GluRIIA RNAi ( w 1118 ;G14-Gal4 / +;UAS-GluRIIA RNAi / + ). ( B ) Quantification of the mean fluorescence intensity of individual GluR puncta reveals GluRIIA subunits are virtually undetectable at NMJs of both GluRIIA mutants and G14 > GluRIIA RNAi , while the essential subunits GluRIIC and GluRIID are moderately reduced, reflecting expression of the remaining GluRIIB-containing receptors. ( C ) Left: Schematic illustrating the composition of GluRIIA-containing and GluRIIB-containing postsynaptic receptor subtypes at the Drosophila NMJ. Right: Quantitative PCR analysis of GluR transcript levels for GluRIIA/B/C/D/E subunits in G14 > GluRIIA RNAi normalized to G14/+ . ( D ) Representative electrophysiological traces of EPSP and mEPSP recordings in the indicated genotypes. ( E–G ) Quantification of mEPSP amplitude ( E ), EPSP amplitude ( F ), and quantal content ( G ) in the indicated genotypes. Note that while mEPSP amplitudes are reduced to similar levels in GluRIIA mutants and G14 > GluRIIA RNAi , EPSP amplitudes remain similar to wild type because of a homeostatic increase in presynaptic glutamate release (quantal content). ( H .
    Figure Legend Snippet: GluRIIA knock down phenocopies GluRIIA mutants. ( A ) Representative images of NMJs on muscle six immunostained with antibodies that recognize GluRIIA, GluRIIC, and GluRIID receptor subunits in wild type ( w 1118 ), GluRIIA mutants ( w 1118 ;GluRIIA SP16 ), and G14 > GluRIIA RNAi ( w 1118 ;G14-Gal4 / +;UAS-GluRIIA RNAi / + ). ( B ) Quantification of the mean fluorescence intensity of individual GluR puncta reveals GluRIIA subunits are virtually undetectable at NMJs of both GluRIIA mutants and G14 > GluRIIA RNAi , while the essential subunits GluRIIC and GluRIID are moderately reduced, reflecting expression of the remaining GluRIIB-containing receptors. ( C ) Left: Schematic illustrating the composition of GluRIIA-containing and GluRIIB-containing postsynaptic receptor subtypes at the Drosophila NMJ. Right: Quantitative PCR analysis of GluR transcript levels for GluRIIA/B/C/D/E subunits in G14 > GluRIIA RNAi normalized to G14/+ . ( D ) Representative electrophysiological traces of EPSP and mEPSP recordings in the indicated genotypes. ( E–G ) Quantification of mEPSP amplitude ( E ), EPSP amplitude ( F ), and quantal content ( G ) in the indicated genotypes. Note that while mEPSP amplitudes are reduced to similar levels in GluRIIA mutants and G14 > GluRIIA RNAi , EPSP amplitudes remain similar to wild type because of a homeostatic increase in presynaptic glutamate release (quantal content). ( H .

    Techniques Used: Fluorescence, Expressing, Real-time Polymerase Chain Reaction

    8) Product Images from "Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization"

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006857

    Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: RNA Expression, Transfection, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Fluorescence

    Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: Expressing, Capillary Electrochromatography, Transfection, Mutagenesis, BAC Assay, Clone Assay, Real-time Polymerase Chain Reaction

    9) Product Images from "Identification of ARKL1 as a Negative Regulator of Epstein-Barr Virus Reactivation"

    Article Title: Identification of ARKL1 as a Negative Regulator of Epstein-Barr Virus Reactivation

    Journal: Journal of Virology

    doi: 10.1128/JVI.00989-19

    ARKL1 overexpression inhibits EBV reactivation in AGS-EBV cells. (A) AGS-EBV cells were transfected with plasmids expressing FLAG-ARKL1 or FLAG-DDX24 and then treated with NaB/TPA. Twenty-four hours later, cells were fixed and stained with antibodies against FLAG and BZLF1, and the percentage of FLAG-positive cells expressing BZLF1 was determined. Untransfected FLAG-negative cells expressing BZLF1 are also shown (Untrans.). At least 100 transfected cells were counted for each experiment. Average values from three separate experiments with standard deviations are shown. Representative images of cells stained with DAPI, FLAG, and BZLF1 are also displayed. (B) AGS-EBV cells were transfected and processed as in (A) except that cells were not treated with NaB/TPA. (C) AGS-EBV cells were transfected with plasmids expressing FLAG-FBXO38, FLAG-BKRF4, FLAG-DDX24, or FLAG-ARKL1, and the percentage of BZLF1-positive cells was compared. (D) AGS-EBV cells were transfected with FLAG-ARKL1 or empty FLAG (pCMV3FC) expression plasmids as in (B) followed by quantification of BZLF1 transcripts by RT-qPCR. BZLF1 mRNA levels were normalized to actin mRNA and shown relative to empty plasmid control. The averages from three experiments along with their standard deviations are shown. **, 0.001
    Figure Legend Snippet: ARKL1 overexpression inhibits EBV reactivation in AGS-EBV cells. (A) AGS-EBV cells were transfected with plasmids expressing FLAG-ARKL1 or FLAG-DDX24 and then treated with NaB/TPA. Twenty-four hours later, cells were fixed and stained with antibodies against FLAG and BZLF1, and the percentage of FLAG-positive cells expressing BZLF1 was determined. Untransfected FLAG-negative cells expressing BZLF1 are also shown (Untrans.). At least 100 transfected cells were counted for each experiment. Average values from three separate experiments with standard deviations are shown. Representative images of cells stained with DAPI, FLAG, and BZLF1 are also displayed. (B) AGS-EBV cells were transfected and processed as in (A) except that cells were not treated with NaB/TPA. (C) AGS-EBV cells were transfected with plasmids expressing FLAG-FBXO38, FLAG-BKRF4, FLAG-DDX24, or FLAG-ARKL1, and the percentage of BZLF1-positive cells was compared. (D) AGS-EBV cells were transfected with FLAG-ARKL1 or empty FLAG (pCMV3FC) expression plasmids as in (B) followed by quantification of BZLF1 transcripts by RT-qPCR. BZLF1 mRNA levels were normalized to actin mRNA and shown relative to empty plasmid control. The averages from three experiments along with their standard deviations are shown. **, 0.001

    Techniques Used: Over Expression, Transfection, Expressing, Staining, Quantitative RT-PCR, Plasmid Preparation

    ARKL1 silencing promotes EBV reactivation in NPC43 and M81 B cells. (A) NPC43 cells were transduced with lentivirus expressing three different shRNAs (A, B, and C) targeting ARKL1 or negative-control shRNA (shControl). Equal amounts of lysates were compared by Western blotting using antibodies against ARKL1, BZLF1, and actin. (B) Quantification of BZLF1 bands from two experiments performed as in (A), showing averages and standard deviations. (C) M81 B cells were transduced with lentivirus expressing three different ARKL1-specific shRNAs or negative-control shRNA, and equal amounts of lysates were compared by Western blotting. (D) Quantification of BZLF1 bands from two experiments performed as in (C), showing averages and standard deviations. (E) M81 B cells transduced with ARKL1 shRNAs or negative-control shRNA as in (C) were harvested for RT-qPCR analysis of BZLF1 mRNA transcripts. Levels of the BZLF1 transcripts normalized to actin are shown relative to the control shRNA.
    Figure Legend Snippet: ARKL1 silencing promotes EBV reactivation in NPC43 and M81 B cells. (A) NPC43 cells were transduced with lentivirus expressing three different shRNAs (A, B, and C) targeting ARKL1 or negative-control shRNA (shControl). Equal amounts of lysates were compared by Western blotting using antibodies against ARKL1, BZLF1, and actin. (B) Quantification of BZLF1 bands from two experiments performed as in (A), showing averages and standard deviations. (C) M81 B cells were transduced with lentivirus expressing three different ARKL1-specific shRNAs or negative-control shRNA, and equal amounts of lysates were compared by Western blotting. (D) Quantification of BZLF1 bands from two experiments performed as in (C), showing averages and standard deviations. (E) M81 B cells transduced with ARKL1 shRNAs or negative-control shRNA as in (C) were harvested for RT-qPCR analysis of BZLF1 mRNA transcripts. Levels of the BZLF1 transcripts normalized to actin are shown relative to the control shRNA.

    Techniques Used: Transduction, Expressing, Negative Control, shRNA, Western Blot, Quantitative RT-PCR

    ARKL1 silencing promotes EBV reactivation in AGS-EBV cells. (A) AGS-EBV cells were treated with siRNAs against ARKL1 or negative-control siRNA, and then reactivation was induced with NaB/TPA for 24 hours. A total of 30 μg of lysates was compared by Western blotting using antibodies against ARKL1, BMRF1, BZLF1, and actin. (B) Quantification of BMRF1 bands from three experiments performed as in (A). (C) AGS-EBV cells were treated with siRNAs against ARKL1 or negative-control siRNA, and then 100 μg of lysates were compared by Western blotting as in (A). (D) Quantification of BZLF1 bands from three experiments performed as in (C). (E) Western blot comparing 30 μg of lysates from induced and uninduced samples from (A and C). Exposure time used was longer than in panel A. (F) AGS-EBV cells were transfected with siRNA targeting ARKL1 or negative-control siRNA as in (C), followed by quantification of BZLF1 transcripts by RT-qPCR. BZLF1 mRNA levels were normalized to actin mRNA and are shown relative to negative-control siRNA. Average values from three separate experiments with standard deviations are shown. *, 0.01
    Figure Legend Snippet: ARKL1 silencing promotes EBV reactivation in AGS-EBV cells. (A) AGS-EBV cells were treated with siRNAs against ARKL1 or negative-control siRNA, and then reactivation was induced with NaB/TPA for 24 hours. A total of 30 μg of lysates was compared by Western blotting using antibodies against ARKL1, BMRF1, BZLF1, and actin. (B) Quantification of BMRF1 bands from three experiments performed as in (A). (C) AGS-EBV cells were treated with siRNAs against ARKL1 or negative-control siRNA, and then 100 μg of lysates were compared by Western blotting as in (A). (D) Quantification of BZLF1 bands from three experiments performed as in (C). (E) Western blot comparing 30 μg of lysates from induced and uninduced samples from (A and C). Exposure time used was longer than in panel A. (F) AGS-EBV cells were transfected with siRNA targeting ARKL1 or negative-control siRNA as in (C), followed by quantification of BZLF1 transcripts by RT-qPCR. BZLF1 mRNA levels were normalized to actin mRNA and are shown relative to negative-control siRNA. Average values from three separate experiments with standard deviations are shown. *, 0.01

    Techniques Used: Negative Control, Western Blot, Transfection, Quantitative RT-PCR

    10) Product Images from "Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction"

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    Journal: Medicine

    doi: 10.1097/MD.0000000000013066

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.
    Figure Legend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction
    Article Snippet: .. 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol. .. The PCR were performed on the LightCycler 480/II machine (Roche, Mannheim, Germany) and the PCR conditions were set as follow: 1 cycle of 10 minutes at 55 °C, 1 cycle of 1 minute at 95 °C, 45 cycles of 10 second at 95 °C and 30 seconds at 60 °C, and 1 cycle of 1 minute at 60 °C finally.

    Article Title: Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
    Article Snippet: .. For analysis of pCaMKII levels, Ib and Is regions were identified using DLG and HRP on muscle 6/7 of segment A2 and A3, and only the pCamKII signal that co-localized with DLG was summated and divided by the bouton area under consideration to obtain average pCamKII. quantitative PCR: quantitative PCR (qPCR) was performed using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions. .. RNA was isolated and prepared from body wall tissue as described previously ( ).

    Polymerase Chain Reaction:

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: .. To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems. .. Primers of lncRNA TUG1 and β-actin were designed and synthesized by GenePharma (Shanghai, China).

    Article Title: Globoside Is Dispensable for Parvovirus B19 Entry but Essential at a Postentry Step for Productive Infection
    Article Snippet: .. For detection of the B3GalNT1 mRNA, the Luna Universal one-step reverse transcription-quantitative PCR (RT-qPCR) kit (New England BioLabs, Ipswich, MA) was used. ..

    Quantitative RT-PCR:

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction
    Article Snippet: .. 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol. .. The PCR were performed on the LightCycler 480/II machine (Roche, Mannheim, Germany) and the PCR conditions were set as follow: 1 cycle of 10 minutes at 55 °C, 1 cycle of 1 minute at 95 °C, 45 cycles of 10 second at 95 °C and 30 seconds at 60 °C, and 1 cycle of 1 minute at 60 °C finally.

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: .. To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems. .. Primers of lncRNA TUG1 and β-actin were designed and synthesized by GenePharma (Shanghai, China).

    Article Title: Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
    Article Snippet: .. For analysis of pCaMKII levels, Ib and Is regions were identified using DLG and HRP on muscle 6/7 of segment A2 and A3, and only the pCamKII signal that co-localized with DLG was summated and divided by the bouton area under consideration to obtain average pCamKII. quantitative PCR: quantitative PCR (qPCR) was performed using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions. .. RNA was isolated and prepared from body wall tissue as described previously ( ).

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB). .. The copy numbers were determined from the calibration curves generated for all three genes based on known concentrations of pRB-1B.

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans
    Article Snippet: .. Quantification of LAC1 mRNA in the samples was achieved through RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB). .. LAC1 primers spanning exon-exon junctions were used in qPCR to ensure exclusive amplification of mRNA.

    Article Title: Globoside Is Dispensable for Parvovirus B19 Entry but Essential at a Postentry Step for Productive Infection
    Article Snippet: .. For detection of the B3GalNT1 mRNA, the Luna Universal one-step reverse transcription-quantitative PCR (RT-qPCR) kit (New England BioLabs, Ipswich, MA) was used. ..

    Article Title: Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing
    Article Snippet: .. RT-qPCR was performed to confirm the silencing of agl -2 gene in a dose-response manner using Luna Universal One-Step RT-qPCR Kit (NEB) following the manufacturer’s protocol. ..

    Expressing:

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction
    Article Snippet: .. 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol. .. The PCR were performed on the LightCycler 480/II machine (Roche, Mannheim, Germany) and the PCR conditions were set as follow: 1 cycle of 10 minutes at 55 °C, 1 cycle of 1 minute at 95 °C, 45 cycles of 10 second at 95 °C and 30 seconds at 60 °C, and 1 cycle of 1 minute at 60 °C finally.

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: .. To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems. .. Primers of lncRNA TUG1 and β-actin were designed and synthesized by GenePharma (Shanghai, China).

    Synthesized:

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction
    Article Snippet: .. 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol. .. The PCR were performed on the LightCycler 480/II machine (Roche, Mannheim, Germany) and the PCR conditions were set as follow: 1 cycle of 10 minutes at 55 °C, 1 cycle of 1 minute at 95 °C, 45 cycles of 10 second at 95 °C and 30 seconds at 60 °C, and 1 cycle of 1 minute at 60 °C finally.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    New England Biolabs luna universal one step rt qpcr kit
    Plasma <t>lncRNA</t> TUG1 was upregulated in osteoporosis patients than in healthy participants. <t>RT-qPCR</t> results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p
    Luna Universal One Step Rt Qpcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna universal one step rt qpcr kit/product/New England Biolabs
    Average 98 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    luna universal one step rt qpcr kit - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    Image Search Results


    Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts

    doi: 10.1186/s13018-019-1430-4

    Figure Lengend Snippet: Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Article Snippet: To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems.

    Techniques: Quantitative RT-PCR

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Journal: Medicine

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    doi: 10.1097/MD.0000000000013066

    Figure Lengend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Article Snippet: 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Effects of external RNA pesticide on inhibiting S. sclerotiorum from causing lesions on canola leaves comparing dsRNA targeting agl -2 at 800 ng/20 μl, 400 ng/20 μl, 200 ng/20 μl, dsRNA targeting agl -4 at 800 ng/20 μl as control (from left to right), confirmed by (A) RT-qPCR with reduced expression levels of agl -2 at 200 and 400 ng, and lesion comparison at (B) 36, (C) 48, and (D) 72 h post-inoculation.

    Journal: Frontiers in Plant Science

    Article Title: Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing

    doi: 10.3389/fpls.2019.00976

    Figure Lengend Snippet: Effects of external RNA pesticide on inhibiting S. sclerotiorum from causing lesions on canola leaves comparing dsRNA targeting agl -2 at 800 ng/20 μl, 400 ng/20 μl, 200 ng/20 μl, dsRNA targeting agl -4 at 800 ng/20 μl as control (from left to right), confirmed by (A) RT-qPCR with reduced expression levels of agl -2 at 200 and 400 ng, and lesion comparison at (B) 36, (C) 48, and (D) 72 h post-inoculation.

    Article Snippet: RT-qPCR was performed to confirm the silencing of agl -2 gene in a dose-response manner using Luna Universal One-Step RT-qPCR Kit (NEB) following the manufacturer’s protocol.

    Techniques: Quantitative RT-PCR, Expressing

    Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Journal: Scientific Reports

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans

    doi: 10.1038/s41598-018-27813-3

    Figure Lengend Snippet: Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Article Snippet: Quantification of LAC1 mRNA in the samples was achieved through RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB).

    Techniques: Expressing, Quantitative RT-PCR