luna universal one step rt qpcr kit  (New England Biolabs)


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    Name:
    Luna Universal One Step RT qPCR Kit
    Description:
    Luna Universal One Step RT qPCR Kit 2 500 rxns
    Catalog Number:
    e3005e
    Price:
    2074
    Size:
    2 500 rxns
    Category:
    RT PCR Kits
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    New England Biolabs luna universal one step rt qpcr kit
    Luna Universal One Step RT qPCR Kit
    Luna Universal One Step RT qPCR Kit 2 500 rxns
    https://www.bioz.com/result/luna universal one step rt qpcr kit/product/New England Biolabs
    Average 95 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    luna universal one step rt qpcr kit - by Bioz Stars, 2020-02
    95/100 stars

    Images

    1) Product Images from "LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts"

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts

    Journal: Journal of Orthopaedic Surgery and Research

    doi: 10.1186/s13018-019-1430-4

    Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p
    Figure Legend Snippet: Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Techniques Used: Quantitative RT-PCR

    2) Product Images from "Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction"

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    Journal: Medicine

    doi: 10.1097/MD.0000000000013066

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.
    Figure Legend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    3) Product Images from "Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans"

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-27813-3

    Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.
    Figure Legend Snippet: Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Techniques Used: Expressing, Quantitative RT-PCR

    4) Product Images from "Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization"

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006857

    Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: RNA Expression, Transfection, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Fluorescence

    Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: Expressing, Capillary Electrochromatography, Transfection, Mutagenesis, BAC Assay, Clone Assay, Real-time Polymerase Chain Reaction

    5) Product Images from "Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation"

    Article Title: Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation

    Journal: eLife

    doi: 10.7554/eLife.34338

    GluRIIA knock down phenocopies GluRIIA mutants. ( A ) Representative images of NMJs on muscle six immunostained with antibodies that recognize GluRIIA, GluRIIC, and GluRIID receptor subunits in wild type ( w 1118 ), GluRIIA mutants ( w 1118 ;GluRIIA SP16 ), and G14 > GluRIIA RNAi ( w 1118 ;G14-Gal4 / +;UAS-GluRIIA RNAi / + ). ( B ) Quantification of the mean fluorescence intensity of individual GluR puncta reveals GluRIIA subunits are virtually undetectable at NMJs of both GluRIIA mutants and G14 > GluRIIA RNAi , while the essential subunits GluRIIC and GluRIID are moderately reduced, reflecting expression of the remaining GluRIIB-containing receptors. ( C ) Left: Schematic illustrating the composition of GluRIIA-containing and GluRIIB-containing postsynaptic receptor subtypes at the Drosophila NMJ. Right: Quantitative PCR analysis of GluR transcript levels for GluRIIA/B/C/D/E subunits in G14 > GluRIIA RNAi normalized to G14/+ . ( D ) Representative electrophysiological traces of EPSP and mEPSP recordings in the indicated genotypes. ( E–G ) Quantification of mEPSP amplitude ( E ), EPSP amplitude ( F ), and quantal content ( G ) in the indicated genotypes. Note that while mEPSP amplitudes are reduced to similar levels in GluRIIA mutants and G14 > GluRIIA RNAi , EPSP amplitudes remain similar to wild type because of a homeostatic increase in presynaptic glutamate release (quantal content). ( H .
    Figure Legend Snippet: GluRIIA knock down phenocopies GluRIIA mutants. ( A ) Representative images of NMJs on muscle six immunostained with antibodies that recognize GluRIIA, GluRIIC, and GluRIID receptor subunits in wild type ( w 1118 ), GluRIIA mutants ( w 1118 ;GluRIIA SP16 ), and G14 > GluRIIA RNAi ( w 1118 ;G14-Gal4 / +;UAS-GluRIIA RNAi / + ). ( B ) Quantification of the mean fluorescence intensity of individual GluR puncta reveals GluRIIA subunits are virtually undetectable at NMJs of both GluRIIA mutants and G14 > GluRIIA RNAi , while the essential subunits GluRIIC and GluRIID are moderately reduced, reflecting expression of the remaining GluRIIB-containing receptors. ( C ) Left: Schematic illustrating the composition of GluRIIA-containing and GluRIIB-containing postsynaptic receptor subtypes at the Drosophila NMJ. Right: Quantitative PCR analysis of GluR transcript levels for GluRIIA/B/C/D/E subunits in G14 > GluRIIA RNAi normalized to G14/+ . ( D ) Representative electrophysiological traces of EPSP and mEPSP recordings in the indicated genotypes. ( E–G ) Quantification of mEPSP amplitude ( E ), EPSP amplitude ( F ), and quantal content ( G ) in the indicated genotypes. Note that while mEPSP amplitudes are reduced to similar levels in GluRIIA mutants and G14 > GluRIIA RNAi , EPSP amplitudes remain similar to wild type because of a homeostatic increase in presynaptic glutamate release (quantal content). ( H .

    Techniques Used: Fluorescence, Expressing, Real-time Polymerase Chain Reaction

    6) Product Images from "Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma"

    Article Title: Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma

    Journal: Clinical Epigenetics

    doi: 10.1186/s13148-019-0758-2

    Histone methyltransferases EHMT1 and EHMT2 are upregulated in olaparib-resistant HGSOC. a Four olaparib resistant clones of PEO1-OR were analyzed by RNA-Seq. Of all enzymes involved in H3K9 methylation, EHMT1 (red squares), and KDM1B (green circles) were significantly changed in all four populations of PEO1-OR relative to PEO1. b – d RT-qPCR analysis of histone methyltransferases EHMT1 and EHMT2 , and zinc-finger gene ZNF644 in PEO1 and PEO1-OR cells (mean ± SD, n = 3, unpaired t test). e Protein lysates from PEO1 and PEO1-OR cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control. f , g Patient-derived HGSOC ascites cells were injected intraperitoneally into NSG mice. After 21-day treatment with olaparib or vehicle control, mice were sacrificed and ascites cells were collected and analyzed by RT-qPCR for EHMT1 and EHMT2 (mRNA expression is normalized to GAPDH and plotted as mean ± SD, n = 3 technical PCR replicates, unpaired test; numbers below bars are mouse ear-tag numbers). h Protein lysates from ascites cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control (ear tag numbers correspond with mRNA data)
    Figure Legend Snippet: Histone methyltransferases EHMT1 and EHMT2 are upregulated in olaparib-resistant HGSOC. a Four olaparib resistant clones of PEO1-OR were analyzed by RNA-Seq. Of all enzymes involved in H3K9 methylation, EHMT1 (red squares), and KDM1B (green circles) were significantly changed in all four populations of PEO1-OR relative to PEO1. b – d RT-qPCR analysis of histone methyltransferases EHMT1 and EHMT2 , and zinc-finger gene ZNF644 in PEO1 and PEO1-OR cells (mean ± SD, n = 3, unpaired t test). e Protein lysates from PEO1 and PEO1-OR cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control. f , g Patient-derived HGSOC ascites cells were injected intraperitoneally into NSG mice. After 21-day treatment with olaparib or vehicle control, mice were sacrificed and ascites cells were collected and analyzed by RT-qPCR for EHMT1 and EHMT2 (mRNA expression is normalized to GAPDH and plotted as mean ± SD, n = 3 technical PCR replicates, unpaired test; numbers below bars are mouse ear-tag numbers). h Protein lysates from ascites cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control (ear tag numbers correspond with mRNA data)

    Techniques Used: Clone Assay, RNA Sequencing Assay, Methylation, Quantitative RT-PCR, Derivative Assay, Injection, Mouse Assay, Expressing, Polymerase Chain Reaction

    7) Product Images from "Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization"

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006857

    Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: RNA Expression, Transfection, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Fluorescence

    Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P
    Figure Legend Snippet: Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Techniques Used: Expressing, Capillary Electrochromatography, Transfection, Mutagenesis, BAC Assay, Clone Assay, Real-time Polymerase Chain Reaction

    8) Product Images from "Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction"

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    Journal: Medicine

    doi: 10.1097/MD.0000000000013066

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.
    Figure Legend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Related Articles

    Centrifugation:

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    Amplification:

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    Article Title: Analysis of expression profiles of long noncoding RNAs and mRNAs in brains of mice infected by rabies virus by RNA sequencing
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    Synthesized:

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    Quantitative RT-PCR:

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    Article Title: LINC01638 silencing inhibits cancer cell proliferation in colorectal adenocarcinoma through interaction with RUNX2
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    Article Title: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
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    Article Title: Conservation of cell-intrinsic immune responses in diverse nonhuman primate species
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    SYBR Green Assay:

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    Incubation:

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    Infection:

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    Expressing:

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    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
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    Article Snippet: RT-qPCR was performed using 200 ng total RNA, a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), and gene specific primers. .. Samples were analyzed using an iCycler iQ Real-Time PCR Detection System (Biorad), and the ΔΔCT method was used to calculate the fold-change in gene expression.

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    Article Snippet: RT-qPCR was performed to confirm the silencing of agl -2 gene in a dose-response manner using Luna Universal One-Step RT-qPCR Kit (NEB) following the manufacturer’s protocol. .. The expression levels of agl -2 and an endogenous control (actin) were evaluated with three biological replicates and four technical replicates each.

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    Article Snippet: Real-time RT-PCR was performed to detect express of the selected lncRNAs using Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, USA) according to manufacturer’s instructions. .. Primers used for validation of lncRNA expression were shown in Table .

    Western Blot:

    Article Title: Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis in Tetrahymena thermophila
    Article Snippet: Confirmation of CPDT1 RNAi was performed by Western blotting analysis of tagged protein levels for a Cpdt1HA strain transformed with the CPDT1 hairpin construct ( ). .. RNA was extracted from cells using TriFAST reagent (VWR, Radnor, PA), and RT-qPCR was performed using the LUNA Universal One-Step RT-qPCR kit (New England BioLabs, Frankfurt, Germany).

    Transformation Assay:

    Article Title: Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis in Tetrahymena thermophila
    Article Snippet: Confirmation of CPDT1 RNAi was performed by Western blotting analysis of tagged protein levels for a Cpdt1HA strain transformed with the CPDT1 hairpin construct ( ). .. RNA was extracted from cells using TriFAST reagent (VWR, Radnor, PA), and RT-qPCR was performed using the LUNA Universal One-Step RT-qPCR kit (New England BioLabs, Frankfurt, Germany).

    Transfection:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: To synchronize infection of permissive cells the transfection mixture was incubated with cells only for 30 min. RNA was isolated 24 h post transfection as described above. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Article Title: Conservation of cell-intrinsic immune responses in diverse nonhuman primate species
    Article Snippet: .. RT-qPCR of select ISGs RT-qPCR of total RNA isolated from the 24-well format poly(I:C) transfections was performed using the Luna Universal One-Step RT-qPCR kit (New England BioLabs, Inc.) according to the manufacturer’s directions. ..

    Cell Culture:

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNAzol® RT RNA Isolation Reagent (GeneCopoeia, Inc., Rockville, MD, USA) was used to extract total RNA from tumor tissues, adjacent healthy tissues and in vitro cultured cells. .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Generated:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: The copy numbers were determined based on calibration curves generated for both genes based on known concentrations of pVITRO2-TagBFP-UL30-EGFP plasmid which was used as a standard. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Plasmid Preparation:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: The copy numbers were determined based on calibration curves generated for both genes based on known concentrations of pVITRO2-TagBFP-UL30-EGFP plasmid which was used as a standard. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Polymerase Chain Reaction:

    Article Title: The lncRNA MIR4435-2HG is upregulated in hepatocellular carcinoma and promotes cancer cell proliferation by upregulating miRNA-487a
    Article Snippet: .. PCR systems were prepared using a Luna Universal One-Step RT-qPCR Kit (NEB). .. To detect the expression of miRNA-487a, an miRNeasy Mini Kit (QIAGEN) was used to extract miRNA.

    Article Title: lncRNA DSCAM-AS1 downregulates miR-216b to promote the migration and invasion of colorectal adenocarcinoma cells
    Article Snippet: .. Reverse transcription was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit , PCR reaction systems were prepared using Luna® Universal One-Step RT-qPCR Kit (NEB) with 18S rRNA as endogenous control. .. In order to detect miR-216b, miRNeasy Kit (Qiagen) was used to extract miRNA.

    Article Title: LINC01638 silencing inhibits cancer cell proliferation in colorectal adenocarcinoma through interaction with RUNX2
    Article Snippet: .. Luna® Universal One-Step RT-qPCR kit [New England Biolabs (NEB), Ipswhich, MA, USA] was used to prepare PCR reaction systems. .. Primers used in PCR reactions were as follows: 5′-AATACATCAGCACTGTTGCCTTT-3′ (upstream) 5′-CTCCATACATACATCTCCAAAAAGT-3′ (downstream) for human lncRNA LINC01638; 5′-GTTATGAAAAACCAAGTAGCCAGGTC-3′ (forward) and 5′-GTAATCTGACTCTGTCCTTGTGGAT-3′ (reverse) for RUNX2; 5′-GACCTCTATGCCAACACAGT-3′ (upstream) and 5′-AGTACTTGCGCTCAGGAGGA-3′ (downstream) for human β-actin.

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: .. To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems. .. Primers of lncRNA TUG1 and β-actin were designed and synthesized by GenePharma (Shanghai, China).

    Article Title: Longitudinal and Cross-Sectional Sampling of Serpentovirus (Nidovirus) Infection in Captive Snakes Reveals High Prevalence, Persistent Infection, and Increased Mortality in Pythons and Divergent Serpentovirus Infection in Boas and Colubrids
    Article Snippet: First, a hemi-nested polymerase chain reaction (PCR) was performed. .. Round 1, a reverse transcription quantitative PCR (RT-qPCR), was performed using Luna Universal One-Step RT-qPCR kit (New England BioLabs).

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Isolation:

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: Real-time quantitative PCR Total RNA was extracted from plasma using RNAzol® RT RNA Isolation Reagent (Sigma-Aldrich). .. To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems.

    Article Title: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
    Article Snippet: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) RNA was isolated using RNAeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. .. RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England BioLabs, Ipswich, MA) on a BioRad CFX96 or Applied Biosystems QuantStudio 6 Flex thermocycler using primers for specific target transcripts; 18s rRNA was examined as a housekeeping gene (Supplementary Table ).

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: To synchronize infection of permissive cells the transfection mixture was incubated with cells only for 30 min. RNA was isolated 24 h post transfection as described above. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Article Title: Conservation of cell-intrinsic immune responses in diverse nonhuman primate species
    Article Snippet: .. RT-qPCR of select ISGs RT-qPCR of total RNA isolated from the 24-well format poly(I:C) transfections was performed using the Luna Universal One-Step RT-qPCR kit (New England BioLabs, Inc.) according to the manufacturer’s directions. ..

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNAzol® RT RNA Isolation Reagent (GeneCopoeia, Inc., Rockville, MD, USA) was used to extract total RNA from tumor tissues, adjacent healthy tissues and in vitro cultured cells. .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Purification:

    Article Title: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
    Article Snippet: Schizont stage parasites were purified from cultures using the percoll-alanine density gradient centrifugation method. .. Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations.

    Article Title: LINC01638 silencing inhibits cancer cell proliferation in colorectal adenocarcinoma through interaction with RUNX2
    Article Snippet: Real-time quantitative PCR (RT-qPCR) A total RNA Purification kit (cat. no. 17200, Norgen Biotek Corp., Thorold, ON, CA) was used to extract total RNA, followed by DNaseI (Thermo Fisher Scientific, Inc., Waltham, MA, USA) digestion and reverse transcription using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit. .. Luna® Universal One-Step RT-qPCR kit [New England Biolabs (NEB), Ipswhich, MA, USA] was used to prepare PCR reaction systems.

    Article Title: Polyamine transporter potABCD is required for virulence of encapsulated but not nonencapsulated Streptococcus pneumoniae
    Article Snippet: Bacterial RNA was purified using a Qiagen RNeasy Mini kit, and collected RNA was treated with RQ1 DNase (Promega) following manufacturer’s protocol. .. RT-qPCR was performed using 200 ng total RNA, a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), and gene specific primers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
    Article Snippet: Paragraph title: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) ... RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England BioLabs, Ipswich, MA) on a BioRad CFX96 or Applied Biosystems QuantStudio 6 Flex thermocycler using primers for specific target transcripts; 18s rRNA was examined as a housekeeping gene (Supplementary Table ).

    Construct:

    Article Title: Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis in Tetrahymena thermophila
    Article Snippet: For the remaining constructs, RT-qPCR was used to assay RNA levels for the appropriate gene (Supplemental Figure S1). .. RNA was extracted from cells using TriFAST reagent (VWR, Radnor, PA), and RT-qPCR was performed using the LUNA Universal One-Step RT-qPCR kit (New England BioLabs, Frankfurt, Germany).

    Capillary Electrochromatography:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: To assess transcription of recoded UL30 genes in the viral background 1 μg of BAC DNA was transfected in duplicates into CEC grown in 6-well plates using polyethylenimine as described above. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Software:

    Article Title: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
    Article Snippet: Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations. .. Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations.

    Real-time Polymerase Chain Reaction:

    Article Title: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
    Article Snippet: .. Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations. .. Data were analyzed with Microsoft Excel and GraphPad software (v.7).

    Article Title: lncRNA DSCAM-AS1 downregulates miR-216b to promote the migration and invasion of colorectal adenocarcinoma cells
    Article Snippet: Paragraph title: Real-time quantitative PCR ... Reverse transcription was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit , PCR reaction systems were prepared using Luna® Universal One-Step RT-qPCR Kit (NEB) with 18S rRNA as endogenous control.

    Article Title: LINC01638 silencing inhibits cancer cell proliferation in colorectal adenocarcinoma through interaction with RUNX2
    Article Snippet: Paragraph title: Real-time quantitative PCR (RT-qPCR) ... Luna® Universal One-Step RT-qPCR kit [New England Biolabs (NEB), Ipswhich, MA, USA] was used to prepare PCR reaction systems.

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts
    Article Snippet: Paragraph title: Real-time quantitative PCR ... To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems.

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: The cDNA was quantified by qPCR using the SYBR Green I system in an AB StepOnePlus Real-time PCR system (Thermo Fisher Scientific). .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Article Title: Longitudinal and Cross-Sectional Sampling of Serpentovirus (Nidovirus) Infection in Captive Snakes Reveals High Prevalence, Persistent Infection, and Increased Mortality in Pythons and Divergent Serpentovirus Infection in Boas and Colubrids
    Article Snippet: .. Round 1, a reverse transcription quantitative PCR (RT-qPCR), was performed using Luna Universal One-Step RT-qPCR kit (New England BioLabs). .. Twelve microliter reactions included a final concentration of 1x Luna Universal One-Step Reaction Mix, 1x Luna WarmStart RT Enzyme Mix, and 0.3 μM of each degenerate serpentovirus primer (BarniPVTF and BarniGGTR; ) mixed with 4 μl of RNA template.

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans
    Article Snippet: Quantification of LAC1 mRNA in the samples was achieved through RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB). .. LAC1 primers spanning exon-exon junctions were used in qPCR to ensure exclusive amplification of mRNA.

    Article Title: Polyamine transporter potABCD is required for virulence of encapsulated but not nonencapsulated Streptococcus pneumoniae
    Article Snippet: Paragraph title: Real time PCR ... RT-qPCR was performed using 200 ng total RNA, a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), and gene specific primers.

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

    RNA Extraction:

    Article Title: The lncRNA MIR4435-2HG is upregulated in hepatocellular carcinoma and promotes cancer cell proliferation by upregulating miRNA-487a
    Article Snippet: Quantitative RT-PCR To detect the expression of MIR4435-2HG, total RNA was extracted using an MPure Total RNA Extraction Kit (117,022,160, MP Biomedicals). .. PCR systems were prepared using a Luna Universal One-Step RT-qPCR Kit (NEB).

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans
    Article Snippet: Paragraph title: RNA extraction and RT-qPCR ... Quantification of LAC1 mRNA in the samples was achieved through RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB).

    RNA Expression:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: Paragraph title: Quantification of RNA expression ... The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    In Vitro:

    Article Title: Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing
    Article Snippet: Paragraph title: In vitro dsRNA Synthesis, Inoculation, and Confirmation of Silencing by RT-qPCR ... RT-qPCR was performed to confirm the silencing of agl -2 gene in a dose-response manner using Luna Universal One-Step RT-qPCR Kit (NEB) following the manufacturer’s protocol.

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNAzol® RT RNA Isolation Reagent (GeneCopoeia, Inc., Rockville, MD, USA) was used to extract total RNA from tumor tissues, adjacent healthy tissues and in vitro cultured cells. .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Spectrophotometry:

    Article Title: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
    Article Snippet: NanoDrop spectrophotometry was performed to confirm the concentration of extracted RNA. .. RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England BioLabs, Ipswich, MA) on a BioRad CFX96 or Applied Biosystems QuantStudio 6 Flex thermocycler using primers for specific target transcripts; 18s rRNA was examined as a housekeeping gene (Supplementary Table ).

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: RNA concentration was determined using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and reverse transcription was performed using a RevertAid RT Reverse Transcription kit (Thermo Fisher Scientific, Inc.) on the RNA samples with an A260 /A280 ratio of between 1.8 and 2.0. .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Produced:

    Article Title: Roles of Argonautes and Dicers on Sclerotinia sclerotiorum Antiviral RNA Silencing
    Article Snippet: As controls, the same volume of water, as well as dsRNA targeting agl -4 were pipetted to surround the agar plug on canola leaves. dsRNA targeting agl -4 was produced the same way as that targeting agl -2 but with primers Ss-Ago4-T7p-F and Ss-Ago4-T7p-R. .. RT-qPCR was performed to confirm the silencing of agl -2 gene in a dose-response manner using Luna Universal One-Step RT-qPCR Kit (NEB) following the manufacturer’s protocol.

    Concentration Assay:

    Article Title: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
    Article Snippet: NanoDrop spectrophotometry was performed to confirm the concentration of extracted RNA. .. RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England BioLabs, Ipswich, MA) on a BioRad CFX96 or Applied Biosystems QuantStudio 6 Flex thermocycler using primers for specific target transcripts; 18s rRNA was examined as a housekeeping gene (Supplementary Table ).

    Article Title: Longitudinal and Cross-Sectional Sampling of Serpentovirus (Nidovirus) Infection in Captive Snakes Reveals High Prevalence, Persistent Infection, and Increased Mortality in Pythons and Divergent Serpentovirus Infection in Boas and Colubrids
    Article Snippet: Round 1, a reverse transcription quantitative PCR (RT-qPCR), was performed using Luna Universal One-Step RT-qPCR kit (New England BioLabs). .. Twelve microliter reactions included a final concentration of 1x Luna Universal One-Step Reaction Mix, 1x Luna WarmStart RT Enzyme Mix, and 0.3 μM of each degenerate serpentovirus primer (BarniPVTF and BarniGGTR; ) mixed with 4 μl of RNA template.

    Article Title: LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1
    Article Snippet: RNA concentration was determined using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and reverse transcription was performed using a RevertAid RT Reverse Transcription kit (Thermo Fisher Scientific, Inc.) on the RNA samples with an A260 /A280 ratio of between 1.8 and 2.0. .. Following these steps, a Luna® Universal One-Step RT-qPCR kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for all PCRs on a CFX96 Touch™ Real-Time PCR Detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    BAC Assay:

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization
    Article Snippet: To assess transcription of recoded UL30 genes in the viral background 1 μg of BAC DNA was transfected in duplicates into CEC grown in 6-well plates using polyethylenimine as described above. .. The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Marker:

    Article Title: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
    Article Snippet: Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations. .. Gene expression levels in Suspended cultures were expressed as fold-change relative to levels in Static cultures, after normalization to the expression of the 60S ribosomal protein L18 as an endogenous control and apical membrane antigen 1 (AMA1) as a parasite maturation marker.

    Staining:

    Article Title: Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis in Tetrahymena thermophila
    Article Snippet: Protein extracts were run on a Mini-PROTEAN TGX Stain-Free 4–20% gradient gel (Bio-Rad, Hercules, CA). .. RNA was extracted from cells using TriFAST reagent (VWR, Radnor, PA), and RT-qPCR was performed using the LUNA Universal One-Step RT-qPCR kit (New England BioLabs, Frankfurt, Germany).

    Gradient Centrifugation:

    Article Title: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
    Article Snippet: Schizont stage parasites were purified from cultures using the percoll-alanine density gradient centrifugation method. .. Expression of mRNA transcripts for selected invasion ligands was determined using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems), using manufacturer’s recommendations.

    RNA Detection:

    Article Title: Longitudinal and Cross-Sectional Sampling of Serpentovirus (Nidovirus) Infection in Captive Snakes Reveals High Prevalence, Persistent Infection, and Increased Mortality in Pythons and Divergent Serpentovirus Infection in Boas and Colubrids
    Article Snippet: Paragraph title: Viral RNA Detection ... Round 1, a reverse transcription quantitative PCR (RT-qPCR), was performed using Luna Universal One-Step RT-qPCR kit (New England BioLabs).

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    New England Biolabs luna universal one step rt qpcr kit
    Plasma <t>lncRNA</t> TUG1 was upregulated in osteoporosis patients than in healthy participants. <t>RT-qPCR</t> results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p
    Luna Universal One Step Rt Qpcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: LncRNA TUG1 was upregulated in osteoporosis and regulates the proliferation and apoptosis of osteoclasts

    doi: 10.1186/s13018-019-1430-4

    Figure Lengend Snippet: Plasma lncRNA TUG1 was upregulated in osteoporosis patients than in healthy participants. RT-qPCR results showed that plasma levels of lncRNA TUG1 were significantly higher in osteoporosis patients than in healthy participants (* p

    Article Snippet: To detect the expression of lncRNA TUG1 and PTEN mRNA, Luna® Universal One-Step RT-qPCR Kit (NEB) was used to prepare PCR reaction systems.

    Techniques: Quantitative RT-PCR

    Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Journal: Medicine

    Article Title: Differential expression of circulating long non-coding RNAs in patients with acute myocardial infarction

    doi: 10.1097/MD.0000000000013066

    Figure Lengend Snippet: Expression level of differentially expressed lncRNAs in NSTEMI and STEMI patients. ENST00000508020.2, LNC_002011, LNC_000303 LNC_000898, ENST00000573866.2, and ENST00000562710.1 was detected by qPCR and normalized by GAPHD expression in STEMI patients and NSTEMI patients (All P values were less than .05). lncRNAs = long noncoding RNAs, NSTEMI = non-ST-elevation myocardial infarction, STEMI = ST-elevation myocardial infarction.

    Article Snippet: 2.6 Validation by real-time quantitative PCR cDNA was synthesized and Real-Time Quantitative PCR (RT-qPCR) of lncRNAs expression level was performed using Luna Universal One-Step RT-qPCR kits (New England Biolabs, MA) according to the manufacturer's protocol.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Journal: Scientific Reports

    Article Title: Genetic Factors and Genotype-Environment Interactions Contribute to Variation in Melanin Production in the Fungal Pathogen Cryptococcus neoformans

    doi: 10.1038/s41598-018-27813-3

    Figure Lengend Snippet: Normalized LAC1 expression in low, intermediate and high nitrosative stress conditions. The abundance of LAC1 mRNA transcripts was quantified in two representative strains from the study population using RT-qPCR. In both strains, the normalized LAC1 expression was correlated with the corresponding level of melanin production. LAC1 expression was induced in intermediate nitrosative stress in comparison to low and high nitrosative stress.

    Article Snippet: Quantification of LAC1 mRNA in the samples was achieved through RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB).

    Techniques: Expressing, Quantitative RT-PCR

    Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Journal: PLoS Pathogens

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    doi: 10.1371/journal.ppat.1006857

    Figure Lengend Snippet: Quantification of RNA expression and protein production from the recoded UL30 genes. HEK 293T cells were transfected with dual expression plasmids pVITRO2-TagBFP-UL30-EGFP that carried differently recoded UL30 genes fused in frame with EGFP gene. 24 h post transfection RNA expression (A) from the recoded genes was quantified by qPCR, and protein production by flow cytometry (B). The UL30 RNA levels were normalized against the TagBFP levels. We used EGFP fluorescence as a reporter to quantify protein production of the fusion UL30-EGFP genes. The EGFP fluorescence was normalized against the TagBFP fluorescence. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Article Snippet: The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Techniques: RNA Expression, Transfection, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Cytometry, Fluorescence

    Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Journal: PLoS Pathogens

    Article Title: Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization

    doi: 10.1371/journal.ppat.1006857

    Figure Lengend Snippet: Characterization of recoded MDV UL30 mutants. (A) Effect of recoding on UL30 expression from the virus background. CEC were transfected with the parental or mutant BAC clones that carried differently recoded UL30 genes. 24 h post transfection RNA levels of UL29, UL30 and UL42 genes were quantified by qPCR. P-values were calculated using Kruskal-Wallis H test, * indicates P

    Article Snippet: The gene-specific primers and probes were used to quantify the viral transcripts of UL29, UL30 and UL42 genes ( ) using the Luna Universal One-Step RT-qPCR Kit (NEB).

    Techniques: Expressing, Capillary Electrochromatography, Transfection, Mutagenesis, BAC Assay, Clone Assay, Real-time Polymerase Chain Reaction