e1700s  (New England Biolabs)


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  • 95
    Name:
    WarmStartLAMP Kit
    Description:

    Catalog Number:
    E1700
    Price:
    861
    Category:
    Other Kits
    Applications:
    DNA Analysis
    Size:
    500 reactions
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    Structured Review

    New England Biolabs e1700s
    WarmStartLAMP Kit

    https://www.bioz.com/result/e1700s/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e1700s - by Bioz Stars, 2021-09
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP
    Article Snippet: .. 2.5 Real-time RT-LAMP Reverse transcription amplification reactions were based on the WarmStart® LAMP kit (DNA & RNA) to convert viral RNA into DNA, which has an optimal temperature of 65℃. ..

    Article Title: Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
    Article Snippet: .. The target sequence was isothermally amplified using a WarmStart® LAMP Kit (DNA and RNA), (Cat # E1700S, New England Biolabs, Notting Hill, Australia) in a 10 µL reactor, as per the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons
    Article Snippet: .. RT-LAMP reaction mixtures RT-LAMP reactions were prepared by mixing 7.5 μl commercial 2x LAMP master mix (NEB E1700L) or our own LAMP mix (40 mM TrisHCl, pH 8.5, 20 mM (NH4)2SO4, 100 mM KCl, 16 mM MgSO4, 0.2% Tween-20, 2.8 mM each dNTP, 16 μg/ml polA LF, and 2.6–7.7 μg/ml RT) with 1.5 μl of 10x primer/beacon master mix (final concentration: 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB, 0.25 μM beacon) and 6 μl of sample and/or water. ..

    Lamp Assay:

    Article Title: Presence and Stability of SARS-CoV-2 on Environmental Currency and Money Cards
    Article Snippet: .. The WarmStart LAMP Kit (Cat# E1700S, New England BioLabs) was utilized using SARS-CoV-2 specific primers[ ] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15, 2020. ..

    Sequencing:

    Article Title: Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
    Article Snippet: .. The target sequence was isothermally amplified using a WarmStart® LAMP Kit (DNA and RNA), (Cat # E1700S, New England Biolabs, Notting Hill, Australia) in a 10 µL reactor, as per the manufacturer’s instructions. ..

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  • 95
    New England Biolabs conventional pcr warmstart lamp kit
    Conventional <t>PCR</t> to distinguish S. frugiperda and S. exigua . (A) PCR with <t>LAMP</t> external primer set FAW_F3 and FAW_B3 produced a 781 bp product from S. frugiperda DNA only. (B) PCR with primer FAW_F3 and alternative reverse primer FAW_UR produed a 501 bp product from S. frugiperda DNA only. (C) PCR with primers BAW_DF and FAW_UR produced a 454 bp product from S. exigua DNA only. Abbreviations are NC (non-template control), FAW (fall armyworm S. frugiperda ), BAW (beet armyworm S. exigua ), TCW (tobacco cutworm S. litura ), ACL (African cotton leafworm S. littoralis ), RAW (rice armyworm Mythimna separata ), and CBW (cotton bollworm Helicoverpa armigera ).
    Conventional Pcr Warmstart Lamp Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/conventional pcr warmstart lamp kit/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    conventional pcr warmstart lamp kit - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs warmstart lamp kit dna and rna
    Barcoding and PCR amplification of <t>RT-LAMP</t> products (A) Overview of COV-ID. Saliva is collected and inactivated prior to RT-LAMP performed with up to 96 individual sample barcoded primers. LAMP reactions are pooled and further amplified via PCR to introduce Illumina adapter sequences and pool-level dual indexes. A single thermal cycler can amplify 96 or 384 such pools and the resulting “super-pool” can be sequenced overnight to detect multiple amplicons from 9,216 or 36,864 individual patient samples (number of reads in parenthesis assume an output of ∼450M reads from a NextSeq 500). (B) Schematic of the RT-LAMP (step I) of COV-ID. Selected numbered intermediates of RT-LAMP reaction are shown to illustrate how the LAMP barcode, shown in yellow, and the P5 and P7 homology sequences (blue and pink, respectively) are introduced in the final LAMP product. Upon generating the dumb-bell intermediate the reaction proceeds through rapid primed and self-primed extensions to form mixture of various <t>DNA</t> amplicons containing sequences for PCR amplification. A more detailed version of the LAMP phase of COV-ID, including specific sequences, is illustrated in Fig. S1 . (C) Conventional RT-LAMP primers (solid lines) or primers modified for COV-ID (dotted lines) were used for RT-LAMP of SARS-CoV-2. The numbers of inactivated SARS-CoV-2 virions per µL is indicated in the color legend. (D) Schematic of the PCR (step II) of COV-ID. Following RT-LAMP, up to 96 reactions are pooled and purified and Illumina libraries are generated directly by PCR with dual-indexed P5 and P7 adapters in preparation for sequencing. (E) COV-ID primers targeting ACTB mRNA were used for RT-LAMP with HeLa total <t>RNA.</t> LAMP was diluted 1:100, amplified via PCR and resolved on 2% agarose gel.
    Warmstart Lamp Kit Dna And Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warmstart lamp kit dna and rna/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    warmstart lamp kit dna and rna - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Conventional PCR to distinguish S. frugiperda and S. exigua . (A) PCR with LAMP external primer set FAW_F3 and FAW_B3 produced a 781 bp product from S. frugiperda DNA only. (B) PCR with primer FAW_F3 and alternative reverse primer FAW_UR produed a 501 bp product from S. frugiperda DNA only. (C) PCR with primers BAW_DF and FAW_UR produced a 454 bp product from S. exigua DNA only. Abbreviations are NC (non-template control), FAW (fall armyworm S. frugiperda ), BAW (beet armyworm S. exigua ), TCW (tobacco cutworm S. litura ), ACL (African cotton leafworm S. littoralis ), RAW (rice armyworm Mythimna separata ), and CBW (cotton bollworm Helicoverpa armigera ).

    Journal: bioRxiv

    Article Title: Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP

    doi: 10.1101/2020.04.07.029678

    Figure Lengend Snippet: Conventional PCR to distinguish S. frugiperda and S. exigua . (A) PCR with LAMP external primer set FAW_F3 and FAW_B3 produced a 781 bp product from S. frugiperda DNA only. (B) PCR with primer FAW_F3 and alternative reverse primer FAW_UR produed a 501 bp product from S. frugiperda DNA only. (C) PCR with primers BAW_DF and FAW_UR produced a 454 bp product from S. exigua DNA only. Abbreviations are NC (non-template control), FAW (fall armyworm S. frugiperda ), BAW (beet armyworm S. exigua ), TCW (tobacco cutworm S. litura ), ACL (African cotton leafworm S. littoralis ), RAW (rice armyworm Mythimna separata ), and CBW (cotton bollworm Helicoverpa armigera ).

    Article Snippet: 2.4 LAMP and conventional PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, MA) used for LAMP assay.

    Techniques: Polymerase Chain Reaction, Produced

    SARS-CoV-2 LAMP-OSD assays. OSD fluorescence measured in real-time during LAMP amplification for NB (A), 5 primer Lamb (B), and Tholoth (C) LAMP-OSD assays are depicted as amplification curves. Presence or absence of OSD fluorescence visually observed at assay endpoint for NB (D), Lamb (E), and Tholoth (F) LAMP-OSD assays are depicted as images of reaction tubes. NB LAMP-OSD assays were seeded with indicated copies of SARS-CoV-2 N RNA or MERS-CoV N RNA or no templates. Lamb and Tholoth LAMP-OSD assays were seeded with indicated copies of gBlock DNA templates.

    Journal: bioRxiv

    Article Title: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes

    doi: 10.1101/2020.04.13.039941

    Figure Lengend Snippet: SARS-CoV-2 LAMP-OSD assays. OSD fluorescence measured in real-time during LAMP amplification for NB (A), 5 primer Lamb (B), and Tholoth (C) LAMP-OSD assays are depicted as amplification curves. Presence or absence of OSD fluorescence visually observed at assay endpoint for NB (D), Lamb (E), and Tholoth (F) LAMP-OSD assays are depicted as images of reaction tubes. NB LAMP-OSD assays were seeded with indicated copies of SARS-CoV-2 N RNA or MERS-CoV N RNA or no templates. Lamb and Tholoth LAMP-OSD assays were seeded with indicated copies of gBlock DNA templates.

    Article Snippet: The buffer was supplemented with 1.4 mM dNTPs, 0.4 M betaine, 6 mM additional MgCl2 , 2.4 μM each of FIP and BIP, 1.2 μM of indicated loop primers, 0.6 μM each of F3 and B3 primers, 16 units of Bst 2.0 DNA polymerase, and 7.5 units of warmstart RTX reverse transcriptase.

    Techniques: Fluorescence, Amplification

    Multiplex LAMP-OSD analysis of SARS-CoV-2 genomic RNA. All assays contained equimolar amount of both Tholoth and NB LAMP primers as well as both Tholoth and NB OSD probes. RNA copies in each reaction are indicated below the respective tube. Control reaction received 23 ng of human genomic DNA. Image of endpoint OSD fluorescence is depicted.

    Journal: bioRxiv

    Article Title: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes

    doi: 10.1101/2020.04.13.039941

    Figure Lengend Snippet: Multiplex LAMP-OSD analysis of SARS-CoV-2 genomic RNA. All assays contained equimolar amount of both Tholoth and NB LAMP primers as well as both Tholoth and NB OSD probes. RNA copies in each reaction are indicated below the respective tube. Control reaction received 23 ng of human genomic DNA. Image of endpoint OSD fluorescence is depicted.

    Article Snippet: The buffer was supplemented with 1.4 mM dNTPs, 0.4 M betaine, 6 mM additional MgCl2 , 2.4 μM each of FIP and BIP, 1.2 μM of indicated loop primers, 0.6 μM each of F3 and B3 primers, 16 units of Bst 2.0 DNA polymerase, and 7.5 units of warmstart RTX reverse transcriptase.

    Techniques: Multiplex Assay, Fluorescence

    LAMP-OSD analysis of SARS-CoV-2 genomic RNA. Indicated copies of SARS-CoV-2 genomic RNA were analyzed using NB, Tholoth, and both 5 primer and 6 primer Lamb LAMP-OSD assays. Negative control assays received 23 ng of human genomic DNA. Images of endpoint OSD fluorescence are depicted.

    Journal: bioRxiv

    Article Title: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes

    doi: 10.1101/2020.04.13.039941

    Figure Lengend Snippet: LAMP-OSD analysis of SARS-CoV-2 genomic RNA. Indicated copies of SARS-CoV-2 genomic RNA were analyzed using NB, Tholoth, and both 5 primer and 6 primer Lamb LAMP-OSD assays. Negative control assays received 23 ng of human genomic DNA. Images of endpoint OSD fluorescence are depicted.

    Article Snippet: The buffer was supplemented with 1.4 mM dNTPs, 0.4 M betaine, 6 mM additional MgCl2 , 2.4 μM each of FIP and BIP, 1.2 μM of indicated loop primers, 0.6 μM each of F3 and B3 primers, 16 units of Bst 2.0 DNA polymerase, and 7.5 units of warmstart RTX reverse transcriptase.

    Techniques: Negative Control, Fluorescence

    The sensitivity of the LAMP assay results in three temperature conditions such as 65, 63, and 61 °C for M. loreyi species detected under (A1, B1, and C1) visible light, (A2, B2, and C2) ultraviolet light with Cyber Green and (A3, B3, and C3) gel electrophoresis. The original pink color of the reaction mixture turned yellow in a positive reaction when the product was formed but remained pink in negative reactions. (D) Conventional and multiplex PCR to distinguish M. loreyi . 794bp amplicon amplified only in M. loreyi and conserved partial sequence of ace1 type acetylcholinesterase gene was targeted as an internal reference. Abbreviations are NTC (non-template control), RAW (rice armyworm Mythimna loreyi ), OAW (oriental armyworm M. separata ), TM (Turnip moth Agrotis segetum ), CBW (cotton bollworm Helicoverpa armigera ), BAW (beet armyworm S. exigua ), FAW (fall armyworm S. frugiperda ), and TCW (tobacco cutworm S. litura )

    Journal: bioRxiv

    Article Title: Development of a species diagnostic molecular tool for an invasive pest, Mythimna loreyi using LAMP

    doi: 10.1101/2020.10.01.323089

    Figure Lengend Snippet: The sensitivity of the LAMP assay results in three temperature conditions such as 65, 63, and 61 °C for M. loreyi species detected under (A1, B1, and C1) visible light, (A2, B2, and C2) ultraviolet light with Cyber Green and (A3, B3, and C3) gel electrophoresis. The original pink color of the reaction mixture turned yellow in a positive reaction when the product was formed but remained pink in negative reactions. (D) Conventional and multiplex PCR to distinguish M. loreyi . 794bp amplicon amplified only in M. loreyi and conserved partial sequence of ace1 type acetylcholinesterase gene was targeted as an internal reference. Abbreviations are NTC (non-template control), RAW (rice armyworm Mythimna loreyi ), OAW (oriental armyworm M. separata ), TM (Turnip moth Agrotis segetum ), CBW (cotton bollworm Helicoverpa armigera ), BAW (beet armyworm S. exigua ), FAW (fall armyworm S. frugiperda ), and TCW (tobacco cutworm S. litura )

    Article Snippet: 2.3 LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, MA) used for LAMP assay.

    Techniques: Lamp Assay, Nucleic Acid Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Amplification, Sequencing

    Barcoding and PCR amplification of RT-LAMP products (A) Overview of COV-ID. Saliva is collected and inactivated prior to RT-LAMP performed with up to 96 individual sample barcoded primers. LAMP reactions are pooled and further amplified via PCR to introduce Illumina adapter sequences and pool-level dual indexes. A single thermal cycler can amplify 96 or 384 such pools and the resulting “super-pool” can be sequenced overnight to detect multiple amplicons from 9,216 or 36,864 individual patient samples (number of reads in parenthesis assume an output of ∼450M reads from a NextSeq 500). (B) Schematic of the RT-LAMP (step I) of COV-ID. Selected numbered intermediates of RT-LAMP reaction are shown to illustrate how the LAMP barcode, shown in yellow, and the P5 and P7 homology sequences (blue and pink, respectively) are introduced in the final LAMP product. Upon generating the dumb-bell intermediate the reaction proceeds through rapid primed and self-primed extensions to form mixture of various DNA amplicons containing sequences for PCR amplification. A more detailed version of the LAMP phase of COV-ID, including specific sequences, is illustrated in Fig. S1 . (C) Conventional RT-LAMP primers (solid lines) or primers modified for COV-ID (dotted lines) were used for RT-LAMP of SARS-CoV-2. The numbers of inactivated SARS-CoV-2 virions per µL is indicated in the color legend. (D) Schematic of the PCR (step II) of COV-ID. Following RT-LAMP, up to 96 reactions are pooled and purified and Illumina libraries are generated directly by PCR with dual-indexed P5 and P7 adapters in preparation for sequencing. (E) COV-ID primers targeting ACTB mRNA were used for RT-LAMP with HeLa total RNA. LAMP was diluted 1:100, amplified via PCR and resolved on 2% agarose gel.

    Journal: medRxiv

    Article Title: COV-ID: A LAMP sequencing approach for high-throughput co-detection of SARS-CoV-2 and influenza virus in human saliva

    doi: 10.1101/2021.04.23.21255523

    Figure Lengend Snippet: Barcoding and PCR amplification of RT-LAMP products (A) Overview of COV-ID. Saliva is collected and inactivated prior to RT-LAMP performed with up to 96 individual sample barcoded primers. LAMP reactions are pooled and further amplified via PCR to introduce Illumina adapter sequences and pool-level dual indexes. A single thermal cycler can amplify 96 or 384 such pools and the resulting “super-pool” can be sequenced overnight to detect multiple amplicons from 9,216 or 36,864 individual patient samples (number of reads in parenthesis assume an output of ∼450M reads from a NextSeq 500). (B) Schematic of the RT-LAMP (step I) of COV-ID. Selected numbered intermediates of RT-LAMP reaction are shown to illustrate how the LAMP barcode, shown in yellow, and the P5 and P7 homology sequences (blue and pink, respectively) are introduced in the final LAMP product. Upon generating the dumb-bell intermediate the reaction proceeds through rapid primed and self-primed extensions to form mixture of various DNA amplicons containing sequences for PCR amplification. A more detailed version of the LAMP phase of COV-ID, including specific sequences, is illustrated in Fig. S1 . (C) Conventional RT-LAMP primers (solid lines) or primers modified for COV-ID (dotted lines) were used for RT-LAMP of SARS-CoV-2. The numbers of inactivated SARS-CoV-2 virions per µL is indicated in the color legend. (D) Schematic of the PCR (step II) of COV-ID. Following RT-LAMP, up to 96 reactions are pooled and purified and Illumina libraries are generated directly by PCR with dual-indexed P5 and P7 adapters in preparation for sequencing. (E) COV-ID primers targeting ACTB mRNA were used for RT-LAMP with HeLa total RNA. LAMP was diluted 1:100, amplified via PCR and resolved on 2% agarose gel.

    Article Snippet: Each 10 μL RT-LAMP reaction mix consisted of 1x Warmstart LAMP 2x Master Mix (NEB Cat. E1700S), 0.7 μM dUTP (Promega Cat. U1191), 1 μM SYTO-9 (Thermo Cat. S34854), 0.1 μL Thermolabile UDG (Enzymatics Cat. G5020L), 1 μL of saliva template and optionally 20 fg of N2 Spike RNA.

    Techniques: Polymerase Chain Reaction, Amplification, Introduce, Modification, Purification, Generated, Sequencing, Agarose Gel Electrophoresis