1700l  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    WarmStart LAMP Kit DNA and RNA
    Description:
    WarmStart LAMP Kit DNA and RNA 500 rxns
    Catalog Number:
    e1700l
    Price:
    844
    Category:
    Thermostable DNA Polymerases
    Size:
    500 rxns
    Buy from Supplier


    Structured Review

    New England Biolabs 1700l
    WarmStart LAMP Kit DNA and RNA
    WarmStart LAMP Kit DNA and RNA 500 rxns
    https://www.bioz.com/result/1700l/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1700l - by Bioz Stars, 2021-03
    96/100 stars

    Images

    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases
    Article Snippet: .. Like RT-PCR, RT-LAMP combines reverse transcription and LAMP assays in the same pot for specific RNA amplification ( ; ). .. In general, LAMP is more robust and inhibitor-tolerant than PCR ( ; ).

    Amplification:

    Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases
    Article Snippet: .. Like RT-PCR, RT-LAMP combines reverse transcription and LAMP assays in the same pot for specific RNA amplification ( ; ). .. In general, LAMP is more robust and inhibitor-tolerant than PCR ( ; ).

    Article Title: opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection
    Article Snippet: .. After 40 min of RT-LAMP amplification, Cas12a cleavage system was mixed with LAMP product by shaking operation. ..

    other:

    Article Title: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes
    Article Snippet: The buffer was supplemented with 1.4 mM dNTPs, 0.4 M betaine, 6 mM additional MgCl2 , 2.4 μM each of FIP and BIP, 1.2 μM of indicated loop primers, 0.6 μM each of F3 and B3 primers, 16 units of Bst 2.0 DNA polymerase, and 7.5 units of warmstart RTX reverse transcriptase.

    Polymerase Chain Reaction:

    Article Title: Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP
    Article Snippet: .. LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, UK) was used for the LAMP assay. ..

    Article Title: Development of a species diagnostic molecular tool for an invasive pest, Mythimna loreyi using LAMP
    Article Snippet: .. 2.3 LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, MA) used for LAMP assay. ..

    Lamp Assay:

    Article Title: Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP
    Article Snippet: .. LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, UK) was used for the LAMP assay. ..

    Article Title: Development of a species diagnostic molecular tool for an invasive pest, Mythimna loreyi using LAMP
    Article Snippet: .. 2.3 LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, MA) used for LAMP assay. ..

    Modification:

    Article Title: A hydrogel beads based platform for single-cell phenotypic analysis and digital molecular detection
    Article Snippet: Gelbead Digital LAMP (gdLAMP) assay The reagents for LAMP were acquired from New England BioLabs if not indicated otherwise. .. Each 20 μL of modified LAMP mix for digital single bacteria LAMP contained 1× isothermal buffer, 6 mM total MgSO4, 1.4 mM dNTP, 640 U/mL Bst 2.0 WarmStart polymerase, 1.6 μM FIB and BIP, 0.2 μM F3 and B3, 0.8 μM LF and LB, 1.5 mg/mL BSA, 1× LAMP dye ( ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs pcr warmstart lamp kit
    The sensitivity of the <t>LAMP</t> assay results in three temperature conditions, such as 65, 63, and 61 °C, for M. loreyi species detected under ( A1 , B1 , and C1 ) visible light, ( A2 , B2 , and C2 ) ultraviolet light with SYBR Green, and ( A3 , B3 , and C3 ) gel electrophoresis. The original pink color of the reaction mixture turned yellow in a positive reaction when the product was formed but remained pink in negative reactions. ( D ) Conventional and multiplex <t>PCR</t> to distinguish M. loreyi . The 794 bp amplicon was amplified only in M. loreyi, and the conserved partial sequence of ace1 type acetylcholinesterase gene was targeted as an internal reference. Abbreviations are NTC (non-template control), RAW (rice armyworm Mythimna loreyi ), OAW (oriental armyworm Mythimna separata ), TM (Turnip moth Agrotis segetum ), CBW (cotton bollworm Helicoverpa armigera ), BAW (beet armyworm Spodoptera exigua ), FAW (fall armyworm Spodoptera frugiperda ), and TCW (tobacco cutworm Spodoptera litura ).
    Pcr Warmstart Lamp Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr warmstart lamp kit/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr warmstart lamp kit - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

    Image Search Results


    The sensitivity of the LAMP assay results in three temperature conditions, such as 65, 63, and 61 °C, for M. loreyi species detected under ( A1 , B1 , and C1 ) visible light, ( A2 , B2 , and C2 ) ultraviolet light with SYBR Green, and ( A3 , B3 , and C3 ) gel electrophoresis. The original pink color of the reaction mixture turned yellow in a positive reaction when the product was formed but remained pink in negative reactions. ( D ) Conventional and multiplex PCR to distinguish M. loreyi . The 794 bp amplicon was amplified only in M. loreyi, and the conserved partial sequence of ace1 type acetylcholinesterase gene was targeted as an internal reference. Abbreviations are NTC (non-template control), RAW (rice armyworm Mythimna loreyi ), OAW (oriental armyworm Mythimna separata ), TM (Turnip moth Agrotis segetum ), CBW (cotton bollworm Helicoverpa armigera ), BAW (beet armyworm Spodoptera exigua ), FAW (fall armyworm Spodoptera frugiperda ), and TCW (tobacco cutworm Spodoptera litura ).

    Journal: Insects

    Article Title: Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP

    doi: 10.3390/insects11110817

    Figure Lengend Snippet: The sensitivity of the LAMP assay results in three temperature conditions, such as 65, 63, and 61 °C, for M. loreyi species detected under ( A1 , B1 , and C1 ) visible light, ( A2 , B2 , and C2 ) ultraviolet light with SYBR Green, and ( A3 , B3 , and C3 ) gel electrophoresis. The original pink color of the reaction mixture turned yellow in a positive reaction when the product was formed but remained pink in negative reactions. ( D ) Conventional and multiplex PCR to distinguish M. loreyi . The 794 bp amplicon was amplified only in M. loreyi, and the conserved partial sequence of ace1 type acetylcholinesterase gene was targeted as an internal reference. Abbreviations are NTC (non-template control), RAW (rice armyworm Mythimna loreyi ), OAW (oriental armyworm Mythimna separata ), TM (Turnip moth Agrotis segetum ), CBW (cotton bollworm Helicoverpa armigera ), BAW (beet armyworm Spodoptera exigua ), FAW (fall armyworm Spodoptera frugiperda ), and TCW (tobacco cutworm Spodoptera litura ).

    Article Snippet: LAMP and PCR WarmStart® LAMP Kit (New England Biolabs, Ipswich, UK) was used for the LAMP assay.

    Techniques: Lamp Assay, SYBR Green Assay, Nucleic Acid Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Amplification, Sequencing

    Schematic of this study. A hydrogel bead (Gelbeads)-based cell analysis platform was developed for (a) digital molecular detection including PCR and LAMP and (b) single-cell phenotypic analysis. The compartmentalization was realized by (c) a disposable centrifugal droplet generation device. The dashed-line arrow indicates that the crosslinked hydrogel network grants the potential of linking cell phenotype with in situ DNA/RNA characterization at single-cell resolution.

    Journal: bioRxiv

    Article Title: A hydrogel beads based platform for single-cell phenotypic analysis and digital molecular detection

    doi: 10.1101/848168

    Figure Lengend Snippet: Schematic of this study. A hydrogel bead (Gelbeads)-based cell analysis platform was developed for (a) digital molecular detection including PCR and LAMP and (b) single-cell phenotypic analysis. The compartmentalization was realized by (c) a disposable centrifugal droplet generation device. The dashed-line arrow indicates that the crosslinked hydrogel network grants the potential of linking cell phenotype with in situ DNA/RNA characterization at single-cell resolution.

    Article Snippet: Each 20 μL of modified LAMP mix for digital single bacteria LAMP contained 1× isothermal buffer, 6 mM total MgSO4, 1.4 mM dNTP, 640 U/mL Bst 2.0 WarmStart polymerase, 1.6 μM FIB and BIP, 0.2 μM F3 and B3, 0.8 μM LF and LB, 1.5 mg/mL BSA, 1× LAMP dye ( ).

    Techniques: Polymerase Chain Reaction, In Situ

    Various paper-based devices used for nucleic acid extraction from human blood samples. (a) Cross section view and operating procedure of a sliding-strip device for E. coli detection in blood plasma (reproduced with permission from Ref. ( Connelly et al., 2015 ), © American Chemical Society 2015). (b) Four-layered paper-based biosensor used to detect E. coli in blood, water and milk samples (left), and disposable tape used for sealing the paper device for LAMP amplification (right) (reproduced with permission from Ref. ( Choi et al., 2016 ), © The Royal Society of Chemistry (2016). (c) Schematic of the interaction of charge-switchable chitosan and nucleic acid in a pH-dependent manner (reproduced with permission from Ref. ( Byrnes et al., 2015 ), © The Royal Society of Chemistry (2015). (d) Operating process of a handheld, lateral flow device for extraction of S. aureus DNA from blood in 3 min (reproduced with permission from Ref. ( Seok et al., 2019 ), © IOP Publishing 2019). (e) Schematic illustration of the working principle of a paper-strip device used for viral RNA extraction from serum sample (reproduced with permission from Ref. ( Batule et al., 2020 ), © Elsevier 2020).

    Journal: Biosensors & Bioelectronics

    Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

    doi: 10.1016/j.bios.2020.112592

    Figure Lengend Snippet: Various paper-based devices used for nucleic acid extraction from human blood samples. (a) Cross section view and operating procedure of a sliding-strip device for E. coli detection in blood plasma (reproduced with permission from Ref. ( Connelly et al., 2015 ), © American Chemical Society 2015). (b) Four-layered paper-based biosensor used to detect E. coli in blood, water and milk samples (left), and disposable tape used for sealing the paper device for LAMP amplification (right) (reproduced with permission from Ref. ( Choi et al., 2016 ), © The Royal Society of Chemistry (2016). (c) Schematic of the interaction of charge-switchable chitosan and nucleic acid in a pH-dependent manner (reproduced with permission from Ref. ( Byrnes et al., 2015 ), © The Royal Society of Chemistry (2015). (d) Operating process of a handheld, lateral flow device for extraction of S. aureus DNA from blood in 3 min (reproduced with permission from Ref. ( Seok et al., 2019 ), © IOP Publishing 2019). (e) Schematic illustration of the working principle of a paper-strip device used for viral RNA extraction from serum sample (reproduced with permission from Ref. ( Batule et al., 2020 ), © Elsevier 2020).

    Article Snippet: Representative methods include loop-mediated isothermal amplification (LAMP) ( ; ; ), nucleic acid sequence-based amplification (NASBA) , strand displacement amplification (SDA) ( ; ), recombinase polymerase amplification (RPA) ( ; ; ), and helicase dependent amplification (HDA) ( ; ; ).

    Techniques: Stripping Membranes, Amplification, RNA Extraction