pcr reactions  (New England Biolabs)


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  • 94
    Name:
    Phusion High Fidelity PCR Kit
    Description:
    Phusion High Fidelity PCR Kit 200 rxns 50 ul vol
    Catalog Number:
    e0553l
    Price:
    248
    Size:
    200 rxns
    Category:
    PCR related Kits
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    Structured Review

    New England Biolabs pcr reactions
    Phusion High Fidelity PCR Kit
    Phusion High Fidelity PCR Kit 200 rxns 50 ul vol
    https://www.bioz.com/result/pcr reactions/product/New England Biolabs
    Average 94 stars, based on 565 article reviews
    Price from $9.99 to $1999.99
    pcr reactions - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Article Title: Merkel Cell Polyomavirus Small T Antigen Induces Cancer and Embryonic Merkel Cell Proliferation in a Transgenic Mouse Model
    Article Snippet: .. F1 animals were genotyped by PCR using the Phusion high fidelity polymerase kit (NEB). .. The F1 generation produced 1 heterozygous male and 2 heterozygous females, which were bred to each other in order to obtain homozygous animals.

    Article Title: Complete Bacteriophage Transfer in a Bacterial Endosymbiont (Wolbachia) Determined by Targeted Genome Capture
    Article Snippet: .. PCR was performed using the Phusion High-Fidelity enzyme kit (New England Biolabs) with 25 ng of genomic DNA from N. vitripennis 4.9 (WOVitB), N. giraulti IntG[12.1]F9(13) (WOVitA1), or no DNA (data not shown). ..

    Article Title: Bioflavonoids promote stable translocations between MLL-AF9 breakpoint cluster regions independent of normal chromosomal context: Model system to screen environmental risks
    Article Snippet: .. For PCR reactions amplifying GFP1-GFP4 across breakpoint junctions, PCR reactions were performed using Phusion High Fidelity PCR kit (New England Biolabs) according to manufacturer’s protocol. .. Each 25 μL PCR reaction contained 1.0 μg template DNA, 1× HF buffer, 15 pmol each primer, 10mM dNTPs, 1 uL Phusion Polymerase.

    Article Title: Novel high-grade serous epithelial ovarian cancer cell lines that reflect the molecular diversity of both the sporadic and hereditary disease
    Article Snippet: .. One microliter of the reverse-transcribed product either diluted (1/10, for TP53 or beta-actin amplification) or not (for BRCA1 amplification) was subjected to standard polymerase chain reaction (PCR) using the Phusion High-Fidelity PCR kit (New England BioLabs, Whitby, ON), according to the manufacturer's instructions. .. Two pairs of primers were used for BRCA1 amplification, one covering exons 13 to 15: forward, 5′-Gactcttctgcccttgagga-3′ and reverse, 5′-ctgttgctcctccacatcaa-3′; and another covering exons 14-16: forward, 5′-Ggcctttctgctgacaagtt-3′ and reverse, 5′-aattctggcttctccctgct-3′.

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Article Title: Halophilic microbial community compositional shift after a rare rainfall in the Atacama Desert
    Article Snippet: .. PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA. .. Barcoded samples were quantified with the Qubit dsDNA HS Assay Kit (Invitrogen), pooled and sequenced on the Illumina MiSeq platform with 250 bp paired-end reads at the Johns Hopkins Genetic Resources Core Facility (GRCF).

    Article Title: A Secreted Effector Protein of Ustilago maydis Guides Maize Leaf Cells to Form Tumors
    Article Snippet: .. For all the cloning work, PCR was performed using Phusion High-Fidelity (New England Biolabs). .. The PCR products of the different genes were cleaned up before digestion using the Wizard SV Gel and PCR Clean-Up System (Promega).

    Sequencing:

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Generated:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Amplification:

    Article Title: A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignancies
    Article Snippet: .. 2.8 Preparation of a Human Full-length CD22 and CD22ΔE12–14 Plasmids The cDNA fragments encoding either full-length CD22 (CD22FL) or truncated CD22 lacking exons 12–14 (CD22ΔE12–14) were generated by PCR amplification using the Phusion High Fidelity PCR Kit (New England Biolabs, catalog no. E0553L) with the following primer sets: full-length Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′CCGG TCTCGAG GATGTTTGAGGATCACATAGTC-3′, and truncation ΔE12–14 Fwd/Rev 5′-CTTGGT GCTAGC ATGCATCTCCTCGGC-3′/5′-CCGGT CTCGAG CCTTTTTATTCCTCAC-3′. .. The correct PCR products (FL: 2541-bp and ΔE12–14: 2208-bp) were ligated into the 8497-bp lentiviral vector pCL6-2AEGwo through the NheI and XhoI restriction sites (underlined) using the Quick Ligase kit (New England Biolabs catalog no. M2200L) following the manufacturer's instructions.

    Article Title: Novel high-grade serous epithelial ovarian cancer cell lines that reflect the molecular diversity of both the sporadic and hereditary disease
    Article Snippet: .. One microliter of the reverse-transcribed product either diluted (1/10, for TP53 or beta-actin amplification) or not (for BRCA1 amplification) was subjected to standard polymerase chain reaction (PCR) using the Phusion High-Fidelity PCR kit (New England BioLabs, Whitby, ON), according to the manufacturer's instructions. .. Two pairs of primers were used for BRCA1 amplification, one covering exons 13 to 15: forward, 5′-Gactcttctgcccttgagga-3′ and reverse, 5′-ctgttgctcctccacatcaa-3′; and another covering exons 14-16: forward, 5′-Ggcctttctgctgacaagtt-3′ and reverse, 5′-aattctggcttctccctgct-3′.

    Article Title: Cell-Specific Establishment of Poliovirus Resistance to an Inhibitor Targeting a Cellular Protein
    Article Snippet: .. A PCR fragment covering 2C-3A genomic region was amplified using a Phusion PCR kit (New England BioLabs) and sequenced using a commercial sequencing service (Genewiz, Inc.). .. Polio replicon assays were performed essentially as described previously , with minor modifications depending on the cell culture used.

    Clone Assay:

    Article Title: A Secreted Effector Protein of Ustilago maydis Guides Maize Leaf Cells to Form Tumors
    Article Snippet: .. For all the cloning work, PCR was performed using Phusion High-Fidelity (New England Biolabs). .. The PCR products of the different genes were cleaned up before digestion using the Wizard SV Gel and PCR Clean-Up System (Promega).

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  • 99
    New England Biolabs phusion high fidelity
    Phusion High Fidelity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity/product/New England Biolabs
    Average 99 stars, based on 206 article reviews
    Price from $9.99 to $1999.99
    phusion high fidelity - by Bioz Stars, 2020-09
    99/100 stars
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