Biobrick Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 2 article reviews
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1) Product Images from "A Biobrick Library for Cloning Custom Eukaryotic Plasmids"
Article Title: A Biobrick Library for Cloning Custom Eukaryotic Plasmids
Journal: PLoS ONE
Figure Legend Snippet: Examples of biobrick assemblies. (A) Structure of a classical plasmid for EGFP expression using 5 biobricks. (B) Structure of bicistronic custom plasmid. 8 biobricks are linked together to make a construct for C-terminal fusions to the blue fluorescent protein, cerulean, using classical restriction enzyme multiple cloning sites (MCS). For illustration, the actin-bundling binding protein sEspin is cloned into the MCS, resulting in a fusion with cerulean. (C) Fluorescence microscopy image of the EGFP construct in panel A, after transient transfection into HEK293 cells. (D) A fluorescence microscopy image of the Cerulean-sEspin fusion construct in panel B, allows the visualisation of stress fiber-like structures in a HEK293T cell. Scale bars are indicated below each image.
Techniques Used: Plasmid Preparation, Expressing, Construct, Clone Assay, Binding Assay, Fluorescence, Microscopy, Transfection
Figure Legend Snippet: The biobrick assembly principle  ,  . (A) Each biobrick part has the same prefix and suffix, containing restriction enzyme sites. (B) Following restriction digests, a two-insert ligation into the biobrick vector results in a biobrick fusion. (C) The new biobrick part regenerates the original prefix and suffix, but contains an in-frame Thr-Arg scar in protein-coding fusions. (D) MS2 binding site concatemers (MS2 BS), built with iterative biobrick assembly, from 1 to 12-copies (4 steps). M = marker (1 kb ladder). The upstream and downstream sequences between the primer annealing sites and the biobricks contribute 312 bp, while each MS2 BS is 39 bp.
Techniques Used: Ligation, Plasmid Preparation, Binding Assay, Marker
Figure Legend Snippet: Multiple Cloning Site (MCS) biobricks. The uniqueness of each cloning site is dependent on whether the remaining biobricks and backbones used for the custom plasmid also contain the restriction site. Blunt end restriction enzymes are represented in italic. These biobricks link classical cloning to the biobrick system.
Techniques Used: Clone Assay, Plasmid Preparation
2) Product Images from "Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits"
Article Title: Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits
Journal: Journal of Biological Engineering
Figure Legend Snippet: Desired BioBrick circuit modifications and approach with BioScaffolds . Every BioBrick standard biological part (a) consists of a DNA sequence embedded between a "prefix" sequence (purple box) and a "suffix" sequence (yellow box). Parts may also contain scars (b) , which form when two parts, such as "A1" and "A2" in (c) are fused together using BioBrick assembly [ 12 ]. In many cases one would like to convert an undesired scar between two parts in a BioBrick assembly into a different part or completely remove it (d) . Our approach is to create a new BioBrick part (the BioScaffold) (e) . The BioScaffold can be assembled into a circuit using BioBrick assembly, but unlike normal BioBricks it can be removed and replaced with a new part (f) . In this paper we develop a single prototype BioScaffold that illustrates how BioScaffolds can be used to either insert parts or remove scars.
Techniques Used: Sequencing
3) Product Images from "Biotechnology by Design: An Introductory Level, Project-Based, Synthetic Biology Laboratory Program for Undergraduate Students †"
Article Title: Biotechnology by Design: An Introductory Level, Project-Based, Synthetic Biology Laboratory Program for Undergraduate Students †
Journal: Journal of Microbiology & Biology Education
Figure Legend Snippet: BioBrick Assembly Methods Flowchart. The flowchart illustrates the stepwise construction of the reporter plasmid using a Back-End Assembly approach. Molecular biology techniques are listed in blue type, and decision points are outlined in red. Verification
Techniques Used: Plasmid Preparation