quick ligation kit  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Quick Blunting and Quick Ligation Kits
    Description:
    Quick Blunting and Quick Ligation Kits 100 rxns
    Catalog Number:
    e0542l
    Price:
    645
    Size:
    100 rxns
    Category:
    DNA Ligation Kits
    Buy from Supplier


    Structured Review

    New England Biolabs quick ligation kit
    Quick Blunting and Quick Ligation Kits
    Quick Blunting and Quick Ligation Kits 100 rxns
    https://www.bioz.com/result/quick ligation kit/product/New England Biolabs
    Average 94 stars, based on 643 article reviews
    Price from $9.99 to $1999.99
    quick ligation kit - by Bioz Stars, 2020-09
    94/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Remodeling the specificity of an endosomal CORVET tether underlies formation of regulated secretory vesicles in the ciliate Tetrahymena thermophila
    Article Snippet: .. The 5′ and 3′UTR amplicons were cloned in the corresponding p6c-myc_Chx vector containing the appropriate RAB gene, by Quick Ligation (New England, Biolabs Inc.) at SacI/BamHI and XhoI/KpnI sites, respectively. .. The final vectors were digested with SacI and KpnI prior to biolistic transformation of Vps8ap-FF-ZZ-expressing cells.

    DNA Ligation:

    Article Title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression
    Article Snippet: .. R0176S) and NsiI (cat. no. R0127S) T4 DNA ligase (Roche, cat. no. 10481220001) Quick blunt-end DNA ligation kit (New England Biolabs, cat.no. .. M2200S) T4 polynucleotide kinase (New England Biolabs, cat. no.M0201S) LB medium (Sigma-Aldrich, cat. no. L3022) LB agar medium (Sigma-Aldrich, cat. no. L2897) MOPS EZ rich defined medium kit (Teknova, cat. no.M2105) Ampicillin, sterile filtered, 100 mg ml−1 (Sigma-Aldrich, cat. no. A5354) Carbenicillin, sterile filtered, 100 mgml−1 (Sigma-Aldrich, cat. no. C1613) Chloramphenicol, sterile filtered, 34 mgml−1 (Sigma-Aldrich, cat. no. C0378) One Shot TOP10 chemically competent Escherichiacoli ( E. coli ) (Invitrogen, cat. no.C4040-03) Escherichia coli MG1655 strain (ATCC, cat.no.

    Ligation:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. Materials ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024)Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: bolA gene involved in curli amyloids and fimbriae production in E. coli: exploring pathways to inhibit biofilm and amyloid formation
    Article Snippet: .. The eluted products were freed from template strands by using the Dpn I enzyme and ligated by using a quick blunt end ligation kit (New England Biolabs). .. The ligated plasmids were transformed into the chemically competent E. coli Top 10 cells.

    Article Title: Remodeling the specificity of an endosomal CORVET tether underlies formation of regulated secretory vesicles in the ciliate Tetrahymena thermophila
    Article Snippet: .. The 5′ and 3′UTR amplicons were cloned in the corresponding p6c-myc_Chx vector containing the appropriate RAB gene, by Quick Ligation (New England, Biolabs Inc.) at SacI/BamHI and XhoI/KpnI sites, respectively. .. The final vectors were digested with SacI and KpnI prior to biolistic transformation of Vps8ap-FF-ZZ-expressing cells.

    Stripping Membranes:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. Materials ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024)Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Polymerase Chain Reaction:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. Materials ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024)Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Lysis:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. Materials ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024)Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression
    Article Snippet: .. R0176S) and NsiI (cat. no. R0127S) T4 DNA ligase (Roche, cat. no. 10481220001) Quick blunt-end DNA ligation kit (New England Biolabs, cat.no. .. M2200S) T4 polynucleotide kinase (New England Biolabs, cat. no.M0201S) LB medium (Sigma-Aldrich, cat. no. L3022) LB agar medium (Sigma-Aldrich, cat. no. L2897) MOPS EZ rich defined medium kit (Teknova, cat. no.M2105) Ampicillin, sterile filtered, 100 mg ml−1 (Sigma-Aldrich, cat. no. A5354) Carbenicillin, sterile filtered, 100 mgml−1 (Sigma-Aldrich, cat. no. C1613) Chloramphenicol, sterile filtered, 34 mgml−1 (Sigma-Aldrich, cat. no. C0378) One Shot TOP10 chemically competent Escherichiacoli ( E. coli ) (Invitrogen, cat. no.C4040-03) Escherichia coli MG1655 strain (ATCC, cat.no.

    Plasmid Preparation:

    Article Title: Remodeling the specificity of an endosomal CORVET tether underlies formation of regulated secretory vesicles in the ciliate Tetrahymena thermophila
    Article Snippet: .. The 5′ and 3′UTR amplicons were cloned in the corresponding p6c-myc_Chx vector containing the appropriate RAB gene, by Quick Ligation (New England, Biolabs Inc.) at SacI/BamHI and XhoI/KpnI sites, respectively. .. The final vectors were digested with SacI and KpnI prior to biolistic transformation of Vps8ap-FF-ZZ-expressing cells.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs quick ligation
    Quick Ligation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick ligation/product/New England Biolabs
    Average 96 stars, based on 640 article reviews
    Price from $9.99 to $1999.99
    quick ligation - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    Image Search Results