ligation  (New England Biolabs)


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    Name:
    Quick Blunting and Quick Ligation Kits
    Description:
    Quick Blunting and Quick Ligation Kits 100 rxns
    Catalog Number:
    E0542L
    Price:
    645
    Category:
    DNA Ligation Kits
    Size:
    100 rxns
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    Structured Review

    New England Biolabs ligation
    Quick Blunting and Quick Ligation Kits
    Quick Blunting and Quick Ligation Kits 100 rxns
    https://www.bioz.com/result/ligation/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ligation - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    DNA Ligation:

    Article Title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression
    Article Snippet: All-purpose HI-LO DNA mass ladder (Bionexus, cat. no.BN2050) Restriction enzymes (all from New England Biolabs): BglII(cat. no. R0144S), BamHI-HF (cat. no. R3136S), EcoRI-HF (cat. no.R3101S), BstXI (cat. no. R0113L), XhoI (cat. no. R0146S), DpnI (cat.no. .. R0176S) and NsiI (cat. no. R0127S) T4 DNA ligase (Roche, cat. no. 10481220001) Quick blunt-end DNA ligation kit (New England Biolabs, cat.no. .. M2200S) T4 polynucleotide kinase (New England Biolabs, cat. no.M0201S) LB medium (Sigma-Aldrich, cat. no. L3022) LB agar medium (Sigma-Aldrich, cat. no. L2897) MOPS EZ rich defined medium kit (Teknova, cat. no.M2105) Ampicillin, sterile filtered, 100 mg ml−1 (Sigma-Aldrich, cat. no. A5354) Carbenicillin, sterile filtered, 100 mgml−1 (Sigma-Aldrich, cat. no. C1613) Chloramphenicol, sterile filtered, 34 mgml−1 (Sigma-Aldrich, cat. no. C0378) One Shot TOP10 chemically competent Escherichiacoli ( E. coli ) (Invitrogen, cat. no.C4040-03) Escherichia coli MG1655 strain (ATCC, cat.no.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression
    Article Snippet: All-purpose HI-LO DNA mass ladder (Bionexus, cat. no.BN2050) Restriction enzymes (all from New England Biolabs): BglII(cat. no. R0144S), BamHI-HF (cat. no. R3136S), EcoRI-HF (cat. no.R3101S), BstXI (cat. no. R0113L), XhoI (cat. no. R0146S), DpnI (cat.no. .. R0176S) and NsiI (cat. no. R0127S) T4 DNA ligase (Roche, cat. no. 10481220001) Quick blunt-end DNA ligation kit (New England Biolabs, cat.no. .. M2200S) T4 polynucleotide kinase (New England Biolabs, cat. no.M0201S) LB medium (Sigma-Aldrich, cat. no. L3022) LB agar medium (Sigma-Aldrich, cat. no. L2897) MOPS EZ rich defined medium kit (Teknova, cat. no.M2105) Ampicillin, sterile filtered, 100 mg ml−1 (Sigma-Aldrich, cat. no. A5354) Carbenicillin, sterile filtered, 100 mgml−1 (Sigma-Aldrich, cat. no. C1613) Chloramphenicol, sterile filtered, 34 mgml−1 (Sigma-Aldrich, cat. no. C0378) One Shot TOP10 chemically competent Escherichiacoli ( E. coli ) (Invitrogen, cat. no.C4040-03) Escherichia coli MG1655 strain (ATCC, cat.no.

    Ligation:

    Article Title: Whole exome sequence analysis of serous borderline tumors of the ovary
    Article Snippet: Before performing adaptor ligation, 5′- and 3′-adaptors were annealed to a final concentration of 15 μM using 1x annealing buffer (100 mM Tris-HCl, pH 8.0, 1 mM NaCl) in a thermal cycler under the following conditions: 95°C for 3 min, decrease 1°C and hold for 3 min successively, until 4°C hold. .. Adaptors were ligated to the dA-tailed DNA fragments using the Quick Ligation Module protocol (New England Biolabs) and 3 μM annealed adaptors. .. Purification was performed between each enzymatic step using the Agencourt AMPure XP system (Beckman Coulter), and eluted into nuclease-free water according to the manufacturer’s instructions.

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of the Vps8 paralogs with mNeon fluorescent tags Two mNeonGreen fluorescent tags were integrated at the C-termini of the macronuclear open-reading frames (ORFs) of VPS8B , VPS8C , VPS8D , VPS8E and VPS8F via homologous recombination using linearized pVPS8B-2mNeon-6myc-Neo4, pVPS8C-2mNeon-6myc-Neo4, pVPS8D-2mNeon-6myc-Neo4, pVPS8E-2mNeon-6myc-Neo4 and pVPS8F-2mNeon-6myc-Neo4 created as follows. .. The C terminal 745 bp, 763 bp, 763 bp, 745 bp and 468 bp from the VPS8B , VPS8C , VPS8D , VPS8E and VPS8F genomic loci (minus the stop codon), respectively, were amplified by PCR and cloned in digested p2mNeon-6myc-Neo4 vector ( ) at the SacI/MluI sites by Quick Ligation (New England Biolabs Inc.). .. Subsequently, the 758 bp, 744 bp and 799 bp 3′ UTRs of VPS8B , VPS8C and VPS8D , respectively, were cloned in the VPS8-specific p2mNeon-6myc-Neo4 vector at the XhoI/ApaI, while the 808 bp, 793 bp 3′ UTRs of VPS8E and VPS8F, were cloned at the EcoRV/XhoI sites.

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: PCR was used to amplify the 5′ ends (730–794 bp minus the stop codon) and the 3′ UTRs (576–794 bp) of VPS16A , VPS16B , VPS18A , VPS18B , VPS18C and VPS18D . .. The 5′ ends and the 3′ UTR amplicons were cloned in the p6c-myc-Chx vector ( ) by Quick Ligation (New England Biolabs Inc.) at the SacI/NheI and XhoI/ApaI sites, respectively. .. VPS33A and VPS33B macronuclear ORFs were PCR-amplified and inserted by In-Fusion cloning (Clontech, Mountain View, CA) in the linearized p6c-myc-Chx vector at the SpeI site.

    Amplification:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of the Vps8 paralogs with mNeon fluorescent tags Two mNeonGreen fluorescent tags were integrated at the C-termini of the macronuclear open-reading frames (ORFs) of VPS8B , VPS8C , VPS8D , VPS8E and VPS8F via homologous recombination using linearized pVPS8B-2mNeon-6myc-Neo4, pVPS8C-2mNeon-6myc-Neo4, pVPS8D-2mNeon-6myc-Neo4, pVPS8E-2mNeon-6myc-Neo4 and pVPS8F-2mNeon-6myc-Neo4 created as follows. .. The C terminal 745 bp, 763 bp, 763 bp, 745 bp and 468 bp from the VPS8B , VPS8C , VPS8D , VPS8E and VPS8F genomic loci (minus the stop codon), respectively, were amplified by PCR and cloned in digested p2mNeon-6myc-Neo4 vector ( ) at the SacI/MluI sites by Quick Ligation (New England Biolabs Inc.). .. Subsequently, the 758 bp, 744 bp and 799 bp 3′ UTRs of VPS8B , VPS8C and VPS8D , respectively, were cloned in the VPS8-specific p2mNeon-6myc-Neo4 vector at the XhoI/ApaI, while the 808 bp, 793 bp 3′ UTRs of VPS8E and VPS8F, were cloned at the EcoRV/XhoI sites.

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

    Polymerase Chain Reaction:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of the Vps8 paralogs with mNeon fluorescent tags Two mNeonGreen fluorescent tags were integrated at the C-termini of the macronuclear open-reading frames (ORFs) of VPS8B , VPS8C , VPS8D , VPS8E and VPS8F via homologous recombination using linearized pVPS8B-2mNeon-6myc-Neo4, pVPS8C-2mNeon-6myc-Neo4, pVPS8D-2mNeon-6myc-Neo4, pVPS8E-2mNeon-6myc-Neo4 and pVPS8F-2mNeon-6myc-Neo4 created as follows. .. The C terminal 745 bp, 763 bp, 763 bp, 745 bp and 468 bp from the VPS8B , VPS8C , VPS8D , VPS8E and VPS8F genomic loci (minus the stop codon), respectively, were amplified by PCR and cloned in digested p2mNeon-6myc-Neo4 vector ( ) at the SacI/MluI sites by Quick Ligation (New England Biolabs Inc.). .. Subsequently, the 758 bp, 744 bp and 799 bp 3′ UTRs of VPS8B , VPS8C and VPS8D , respectively, were cloned in the VPS8-specific p2mNeon-6myc-Neo4 vector at the XhoI/ApaI, while the 808 bp, 793 bp 3′ UTRs of VPS8E and VPS8F, were cloned at the EcoRV/XhoI sites.

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

    Clone Assay:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of the Vps8 paralogs with mNeon fluorescent tags Two mNeonGreen fluorescent tags were integrated at the C-termini of the macronuclear open-reading frames (ORFs) of VPS8B , VPS8C , VPS8D , VPS8E and VPS8F via homologous recombination using linearized pVPS8B-2mNeon-6myc-Neo4, pVPS8C-2mNeon-6myc-Neo4, pVPS8D-2mNeon-6myc-Neo4, pVPS8E-2mNeon-6myc-Neo4 and pVPS8F-2mNeon-6myc-Neo4 created as follows. .. The C terminal 745 bp, 763 bp, 763 bp, 745 bp and 468 bp from the VPS8B , VPS8C , VPS8D , VPS8E and VPS8F genomic loci (minus the stop codon), respectively, were amplified by PCR and cloned in digested p2mNeon-6myc-Neo4 vector ( ) at the SacI/MluI sites by Quick Ligation (New England Biolabs Inc.). .. Subsequently, the 758 bp, 744 bp and 799 bp 3′ UTRs of VPS8B , VPS8C and VPS8D , respectively, were cloned in the VPS8-specific p2mNeon-6myc-Neo4 vector at the XhoI/ApaI, while the 808 bp, 793 bp 3′ UTRs of VPS8E and VPS8F, were cloned at the EcoRV/XhoI sites.

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: PCR was used to amplify the 5′ ends (730–794 bp minus the stop codon) and the 3′ UTRs (576–794 bp) of VPS16A , VPS16B , VPS18A , VPS18B , VPS18C and VPS18D . .. The 5′ ends and the 3′ UTR amplicons were cloned in the p6c-myc-Chx vector ( ) by Quick Ligation (New England Biolabs Inc.) at the SacI/NheI and XhoI/ApaI sites, respectively. .. VPS33A and VPS33B macronuclear ORFs were PCR-amplified and inserted by In-Fusion cloning (Clontech, Mountain View, CA) in the linearized p6c-myc-Chx vector at the SpeI site.

    Plasmid Preparation:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of the Vps8 paralogs with mNeon fluorescent tags Two mNeonGreen fluorescent tags were integrated at the C-termini of the macronuclear open-reading frames (ORFs) of VPS8B , VPS8C , VPS8D , VPS8E and VPS8F via homologous recombination using linearized pVPS8B-2mNeon-6myc-Neo4, pVPS8C-2mNeon-6myc-Neo4, pVPS8D-2mNeon-6myc-Neo4, pVPS8E-2mNeon-6myc-Neo4 and pVPS8F-2mNeon-6myc-Neo4 created as follows. .. The C terminal 745 bp, 763 bp, 763 bp, 745 bp and 468 bp from the VPS8B , VPS8C , VPS8D , VPS8E and VPS8F genomic loci (minus the stop codon), respectively, were amplified by PCR and cloned in digested p2mNeon-6myc-Neo4 vector ( ) at the SacI/MluI sites by Quick Ligation (New England Biolabs Inc.). .. Subsequently, the 758 bp, 744 bp and 799 bp 3′ UTRs of VPS8B , VPS8C and VPS8D , respectively, were cloned in the VPS8-specific p2mNeon-6myc-Neo4 vector at the XhoI/ApaI, while the 808 bp, 793 bp 3′ UTRs of VPS8E and VPS8F, were cloned at the EcoRV/XhoI sites.

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: PCR was used to amplify the 5′ ends (730–794 bp minus the stop codon) and the 3′ UTRs (576–794 bp) of VPS16A , VPS16B , VPS18A , VPS18B , VPS18C and VPS18D . .. The 5′ ends and the 3′ UTR amplicons were cloned in the p6c-myc-Chx vector ( ) by Quick Ligation (New England Biolabs Inc.) at the SacI/NheI and XhoI/ApaI sites, respectively. .. VPS33A and VPS33B macronuclear ORFs were PCR-amplified and inserted by In-Fusion cloning (Clontech, Mountain View, CA) in the linearized p6c-myc-Chx vector at the SpeI site.

    Incubation:

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

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    New England Biolabs quick ligation module protocol
    Quick Ligation Module Protocol, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick ligation module protocol/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick ligation module protocol - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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