ligation  (New England Biolabs)


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    Name:
    Quick Blunting and Quick Ligation Kits
    Description:
    Quick Blunting and Quick Ligation Kits 100 rxns
    Catalog Number:
    e0542l
    Price:
    645
    Category:
    DNA Ligation Kits
    Size:
    100 rxns
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    Structured Review

    New England Biolabs ligation
    Quick Blunting and Quick Ligation Kits
    Quick Blunting and Quick Ligation Kits 100 rxns
    https://www.bioz.com/result/ligation/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ligation - by Bioz Stars, 2021-03
    95/100 stars

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    Related Articles

    DNA Ligation:

    Article Title: A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons
    Article Snippet: The product was purified with 1.8x Agencourt AMPure XP beads (Beckman Colter, A63881) and then poly-A tailed in dATP (NEB, N0440S) and Klenow (3′–5′ exo-) (NEB, M0212L) in NEB buffer 2 (NEB, B7002S) at 37 °C for 30 min followed by another 1.8x AMPure bead purification step. .. The product was ligated to NEBnext adapters for Illumina (NEB, E7337A) with Quick DNA ligase (NEB, M2200L) in 2x Quick DNA ligation buffer at room temperature for 1–2 h, trimmed with USER enzyme (NEB, E7338A) at 37 °C for 15 min, and subsequently purified with 0.8x AMPure beads. .. The adapter-ligated ChIP–DNA was then PCR-amplified using 2x Phusion HF Master mix (NEB, M0531L) and NEBNext Multiplex Oligos for Illumina, Index Primers Set 1 (NEB, E7335L) with the steps described in Table .

    Purification:

    Article Title: A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons
    Article Snippet: The product was purified with 1.8x Agencourt AMPure XP beads (Beckman Colter, A63881) and then poly-A tailed in dATP (NEB, N0440S) and Klenow (3′–5′ exo-) (NEB, M0212L) in NEB buffer 2 (NEB, B7002S) at 37 °C for 30 min followed by another 1.8x AMPure bead purification step. .. The product was ligated to NEBnext adapters for Illumina (NEB, E7337A) with Quick DNA ligase (NEB, M2200L) in 2x Quick DNA ligation buffer at room temperature for 1–2 h, trimmed with USER enzyme (NEB, E7338A) at 37 °C for 15 min, and subsequently purified with 0.8x AMPure beads. .. The adapter-ligated ChIP–DNA was then PCR-amplified using 2x Phusion HF Master mix (NEB, M0531L) and NEBNext Multiplex Oligos for Illumina, Index Primers Set 1 (NEB, E7335L) with the steps described in Table .

    Ligation:

    Article Title: Somatic mutations in the DNA repairome in prostate cancers in African Americans and Caucasians
    Article Snippet: In brief, sheared DNA samples were purified with Agencourt AMPure XP magnetic Beads. .. DNA ends were repaired with an End it DNA repair kit (Epicentre Madison, WI), and adenine bases were added to the ends of repaired fragments (Ligation kit, NEB Ipswich, MA). .. Samples were purified with Agencourt AMPure XP magnetic beads, and indexing-specific paired-end adapters were ligated.

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of Vps8c and Vps8e with mCherry in Vps8a–mNeon- and Vps8c–mNeon-expressing cells 3mCherry2HA tag was integrated at the C-termini of VPS8C and VPS8E macronuclear ORFs by homologous recombination using pVPS8C-3mCherry2HA-Chx and pVPS8E-3mCherry2HA-Chx. .. To construct the pVPS8C-3mCherry2HA-Chx, the 3FLAG-ZZ-Neo4 fragment in pVPS8C-FLAG-ZZ-Neo4 was replaced with 4491 bp of the 3mCherry2HA-Chx fragment digested with NheI and XhoI from pVPS11-3mCherry2HA-Chx (see below), and cloned at the corresponding sites in pVPS8C-FLAG-ZZ-Neo4 by Quick Ligation (New England Biolabs Inc.) pVPS8E-3mCherry2HA-Chx was obtained by first cloning the 2742 bp of the 3mCherry2HA-3′UTR-BTU1 fragment from pVPS8C-3mCherry2HA-Chx at the NheI and PstI sites in pVPS8E-FLAG-ZZ-Neo4, and then by replacing the Neo4 drug resistance cassette with the PCR-amplified Chx cassette at the PstI and EcoRV sites via Quick Ligation. .. The final constructs were then linearized with SacI and KpnI, and pVPS8C-3mCherry2HA-Chx was biolistically transfected into Vps8a-mNeon-expressing cells, while linearized pVPS8E-3mCherry2HA-Chx was transfected into Vps8c-mNeon and Vps8a-mNeon expressing cells.

    Article Title: Whole exome sequence analysis of serous borderline tumors of the ovary
    Article Snippet: Before performing adaptor ligation, 5′- and 3′-adaptors were annealed to a final concentration of 15 μM using 1x annealing buffer (100 mM Tris-HCl, pH 8.0, 1 mM NaCl) in a thermal cycler under the following conditions: 95°C for 3 min, decrease 1°C and hold for 3 min successively, until 4°C hold. .. Adaptors were ligated to the dA-tailed DNA fragments using the Quick Ligation Module protocol (New England Biolabs) and 3 μM annealed adaptors. .. Purification was performed between each enzymatic step using the Agencourt AMPure XP system (Beckman Coulter), and eluted into nuclease-free water according to the manufacturer’s instructions.

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: Utilizing this optimized high-throughput format results in a 10 fold cost reduction, compared to commercially available kits, making per library or pooled sample costs ~$12.6–14.9 for individually prepared libraries and ~$8.6–10.6 for pooled libraries with individual barcodes; both techniques allow for up to 144 samples to be pooled on a single lane with the barcodes tested herein. .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Article Title: Phage as a Template to Grow Bone Mineral Nanocrystals
    Article Snippet: 15 For convenience purpose, stored competent cells with glycerol at −80 °C can be used for transformation; however, freshly prepared TG1 competent cells will increase the chance of successful transformation of recombinant vectors into cells and is recommended for this protocol. .. 16 Use T4 DNA ligase with a high concentration (2,000,000 units/mL, NEB) for a quick ligation (10 min) at room temperature. .. Avoid a longer reaction time (like 2 h) or a higher temperature (37 °C) for the ligation when a higher concentration of T4 ligase (2,000,000 units/mL, NEB) is used.

    Article Title: A Flexible Template Boundary Element in the RNA Subunit of Fission Yeast Telomerase *A Flexible Template Boundary Element in the RNA Subunit of Fission Yeast Telomerase * S⃞
    Article Snippet: S. pombe telomeres were cloned from genomic DNA samples using the G overhang capture assay ( ). .. In brief, a partial duplex (0.5 pmol) comprised of DNA oligonucleotides PBoli733 (gcgtacgactcactgtagatnnnnn-3′-O(CH2 )2 CH2 OH) and PBoli749 (5′-phosphate-atctacagtgagtcgtacgcaa-3′ biotin) was incubated with 1 μg of S. pombe genomic DNA in a “Quick Ligation” reaction (New England Biolabs). .. Products were digested with EcoRI (40 units) for 3 h at 37 °C, and terminal DNA fragments ligated to the biotinylated tag were recovered on magnetic streptavidin beads (Dynal).

    Construct:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of Vps8c and Vps8e with mCherry in Vps8a–mNeon- and Vps8c–mNeon-expressing cells 3mCherry2HA tag was integrated at the C-termini of VPS8C and VPS8E macronuclear ORFs by homologous recombination using pVPS8C-3mCherry2HA-Chx and pVPS8E-3mCherry2HA-Chx. .. To construct the pVPS8C-3mCherry2HA-Chx, the 3FLAG-ZZ-Neo4 fragment in pVPS8C-FLAG-ZZ-Neo4 was replaced with 4491 bp of the 3mCherry2HA-Chx fragment digested with NheI and XhoI from pVPS11-3mCherry2HA-Chx (see below), and cloned at the corresponding sites in pVPS8C-FLAG-ZZ-Neo4 by Quick Ligation (New England Biolabs Inc.) pVPS8E-3mCherry2HA-Chx was obtained by first cloning the 2742 bp of the 3mCherry2HA-3′UTR-BTU1 fragment from pVPS8C-3mCherry2HA-Chx at the NheI and PstI sites in pVPS8E-FLAG-ZZ-Neo4, and then by replacing the Neo4 drug resistance cassette with the PCR-amplified Chx cassette at the PstI and EcoRV sites via Quick Ligation. .. The final constructs were then linearized with SacI and KpnI, and pVPS8C-3mCherry2HA-Chx was biolistically transfected into Vps8a-mNeon-expressing cells, while linearized pVPS8E-3mCherry2HA-Chx was transfected into Vps8c-mNeon and Vps8a-mNeon expressing cells.

    Clone Assay:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of Vps8c and Vps8e with mCherry in Vps8a–mNeon- and Vps8c–mNeon-expressing cells 3mCherry2HA tag was integrated at the C-termini of VPS8C and VPS8E macronuclear ORFs by homologous recombination using pVPS8C-3mCherry2HA-Chx and pVPS8E-3mCherry2HA-Chx. .. To construct the pVPS8C-3mCherry2HA-Chx, the 3FLAG-ZZ-Neo4 fragment in pVPS8C-FLAG-ZZ-Neo4 was replaced with 4491 bp of the 3mCherry2HA-Chx fragment digested with NheI and XhoI from pVPS11-3mCherry2HA-Chx (see below), and cloned at the corresponding sites in pVPS8C-FLAG-ZZ-Neo4 by Quick Ligation (New England Biolabs Inc.) pVPS8E-3mCherry2HA-Chx was obtained by first cloning the 2742 bp of the 3mCherry2HA-3′UTR-BTU1 fragment from pVPS8C-3mCherry2HA-Chx at the NheI and PstI sites in pVPS8E-FLAG-ZZ-Neo4, and then by replacing the Neo4 drug resistance cassette with the PCR-amplified Chx cassette at the PstI and EcoRV sites via Quick Ligation. .. The final constructs were then linearized with SacI and KpnI, and pVPS8C-3mCherry2HA-Chx was biolistically transfected into Vps8a-mNeon-expressing cells, while linearized pVPS8E-3mCherry2HA-Chx was transfected into Vps8c-mNeon and Vps8a-mNeon expressing cells.

    Polymerase Chain Reaction:

    Article Title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
    Article Snippet: Endogenous tagging of Vps8c and Vps8e with mCherry in Vps8a–mNeon- and Vps8c–mNeon-expressing cells 3mCherry2HA tag was integrated at the C-termini of VPS8C and VPS8E macronuclear ORFs by homologous recombination using pVPS8C-3mCherry2HA-Chx and pVPS8E-3mCherry2HA-Chx. .. To construct the pVPS8C-3mCherry2HA-Chx, the 3FLAG-ZZ-Neo4 fragment in pVPS8C-FLAG-ZZ-Neo4 was replaced with 4491 bp of the 3mCherry2HA-Chx fragment digested with NheI and XhoI from pVPS11-3mCherry2HA-Chx (see below), and cloned at the corresponding sites in pVPS8C-FLAG-ZZ-Neo4 by Quick Ligation (New England Biolabs Inc.) pVPS8E-3mCherry2HA-Chx was obtained by first cloning the 2742 bp of the 3mCherry2HA-3′UTR-BTU1 fragment from pVPS8C-3mCherry2HA-Chx at the NheI and PstI sites in pVPS8E-FLAG-ZZ-Neo4, and then by replacing the Neo4 drug resistance cassette with the PCR-amplified Chx cassette at the PstI and EcoRV sites via Quick Ligation. .. The final constructs were then linearized with SacI and KpnI, and pVPS8C-3mCherry2HA-Chx was biolistically transfected into Vps8a-mNeon-expressing cells, while linearized pVPS8E-3mCherry2HA-Chx was transfected into Vps8c-mNeon and Vps8a-mNeon expressing cells.

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: Utilizing this optimized high-throughput format results in a 10 fold cost reduction, compared to commercially available kits, making per library or pooled sample costs ~$12.6–14.9 for individually prepared libraries and ~$8.6–10.6 for pooled libraries with individual barcodes; both techniques allow for up to 144 samples to be pooled on a single lane with the barcodes tested herein. .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Incubation:

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

    Article Title: A Flexible Template Boundary Element in the RNA Subunit of Fission Yeast Telomerase *A Flexible Template Boundary Element in the RNA Subunit of Fission Yeast Telomerase * S⃞
    Article Snippet: S. pombe telomeres were cloned from genomic DNA samples using the G overhang capture assay ( ). .. In brief, a partial duplex (0.5 pmol) comprised of DNA oligonucleotides PBoli733 (gcgtacgactcactgtagatnnnnn-3′-O(CH2 )2 CH2 OH) and PBoli749 (5′-phosphate-atctacagtgagtcgtacgcaa-3′ biotin) was incubated with 1 μg of S. pombe genomic DNA in a “Quick Ligation” reaction (New England Biolabs). .. Products were digested with EcoRI (40 units) for 3 h at 37 °C, and terminal DNA fragments ligated to the biotinylated tag were recovered on magnetic streptavidin beads (Dynal).

    Amplification:

    Article Title: Viral proteins as a potential driver of histone depletion in dinoflagellates
    Article Snippet: DNA fragmentation and concentration were assessed using a 1% agarose gel containing syto60 dye (Invitrogen) and a high sensitivity Qubit fluorometer (Thermo Fisher), respectively. .. Briefly, samples were end repaired (1X T4 DNA ligase buffer (NEB), 0.4 mM dNTP mix, 2.25 U T4 DNA polymerase (NEB), 0.75 U Klenow DNA polymerase (NEB), and 7.5 U of T4 polynucleotide kinase (NEB), incubated at room temperature for 30 min), A-tailed (1X NEB buffer 2, 0.4 mM dATP, and 3.75 U of Klenow (exo-) (NEB) incubated at 37 °C for 30 min), ligated to adapters (1X Quick DNA ligase buffer (NEB), 1 mM Illumina PE adapters, and 1600 U Quick DNA-ligase (NEB), incubated at room temperature for 1 h) and PCR amplified (1X NEBNext master mix (NEB) and 0.4 μM indexed primers (Illumina)) using 12 PCR cycles with a 65 °C annealing temperature and a 30 s extension time. .. Cells were grown as for ChIP (see Chromatin immunoprecipitation) before being normalized to 25 ODUs.

    Stripping Membranes:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: Utilizing this optimized high-throughput format results in a 10 fold cost reduction, compared to commercially available kits, making per library or pooled sample costs ~$12.6–14.9 for individually prepared libraries and ~$8.6–10.6 for pooled libraries with individual barcodes; both techniques allow for up to 144 samples to be pooled on a single lane with the barcodes tested herein. .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Lysis:

    Article Title: A cost-effective method for high-throughput construction of Illumina sequencing libraries
    Article Snippet: Utilizing this optimized high-throughput format results in a 10 fold cost reduction, compared to commercially available kits, making per library or pooled sample costs ~$12.6–14.9 for individually prepared libraries and ~$8.6–10.6 for pooled libraries with individual barcodes; both techniques allow for up to 144 samples to be pooled on a single lane with the barcodes tested herein. .. ⅛ in diameter stainless steel ball bearing (Hartford Technologies 034-008-2C) 1.1 ml tube in strips of 8 (USA Scientific 1212-8000) 8-cap strip for 1.1 ml racked tubes USA (Scientific 1294-0800) 96-Well Collection Plate (Zymo Research C2002) Agarose I (Amresco 0710-100G) Cell & Lysis Buffer (Epicentre MTC096H) DNA Clean & Concentrator Kit-5 (Zymo Research D4004) Klenow exo- (NEB M0212L) MPC Buffer (Epicentre MMP03750) NEBuffer 2 (NEB B7002S) NEBNext dsDNA Fragmentase (NEB M0348S) O’GeneRuler Plus 100bp Ladder (SM0323) PCR Sealing Mat for 96 Well PCR Plate (USA Scientific 1400-9605) Phusion DNA Polymerase (NEB M0530S) Proteinase K (Qiagen 19133) Qubit dsDNA HS Assay Kit (Life Technologies ) Quick Blunting Kit (NEB E0542S) Quick Ligation Kit (NEB M2200S) SafeView (abm G108) TempPlate No-Skirt 0.2mL PCR Plates, Natural (USA Scientific 1402-9596) ZR-96 DNA Clean & Concentrator Kit-5 (Zymo Research D4023 or D4024) Alternatively, Agencourt AMPure XP beads (Beckman Coulter , , or ) can be substituted for all Zymo Research Clean & Concentrator kits. ..

    Concentration Assay:

    Article Title: Phage as a Template to Grow Bone Mineral Nanocrystals
    Article Snippet: 15 For convenience purpose, stored competent cells with glycerol at −80 °C can be used for transformation; however, freshly prepared TG1 competent cells will increase the chance of successful transformation of recombinant vectors into cells and is recommended for this protocol. .. 16 Use T4 DNA ligase with a high concentration (2,000,000 units/mL, NEB) for a quick ligation (10 min) at room temperature. .. Avoid a longer reaction time (like 2 h) or a higher temperature (37 °C) for the ligation when a higher concentration of T4 ligase (2,000,000 units/mL, NEB) is used.

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  • 95
    New England Biolabs quick dna ligase
    Quick Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick dna ligase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick dna ligase - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

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