bovine tnf α do it yourself elisa  (Kingfisher Biotech)


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    Name:
    Bovine TNF alpha Do It Yourself ELISA
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    Catalog Number:
    DIY0675B-003
    Price:
    700.0
    Quantity:
    1 Set
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    Kingfisher Biotech bovine tnf α do it yourself elisa
    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and <t>TNF-α</t> ( e ) concentrations in culture supernatants were determined by <t>ELISA.</t> ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    https://www.bioz.com/result/bovine tnf α do it yourself elisa/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine tnf α do it yourself elisa - by Bioz Stars, 2021-09
    94/100 stars

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    1) Product Images from "Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression"

    Article Title: Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-80251-y

    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.
    Figure Legend Snippet: Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Techniques Used: Inhibition, Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Concentration Assay

    2) Product Images from "Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease"

    Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00910-17

    Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.
    Figure Legend Snippet: Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.

    Techniques Used: Functional Assay, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).
    Figure Legend Snippet: Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).

    Techniques Used: Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing

    Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.
    Figure Legend Snippet: Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.

    Techniques Used: Activation Assay, Infection, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

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    Article Snippet: .. Tumor necrosis factor-α The spent media collected after cell incubation was analyzed for concentrations of TNF-α using a bovine TNF-α commercial kit (VetSet Elisa Development Kit; Kingfisher Biotech Inc., St. Paul, MN) following the manufacturer's protocol with some modifications. ..

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Choline Regulates the Function of Bovine Immune Cells and Alters the mRNA Abundance of Enzymes and Receptors Involved in Its Metabolism in vitro
    Article Snippet: .. Tumor necrosis factor-α The spent media collected after cell incubation was analyzed for concentrations of TNF-α using a bovine TNF-α commercial kit (VetSet Elisa Development Kit; Kingfisher Biotech Inc., St. Paul, MN) following the manufacturer's protocol with some modifications. ..

    Article Title: The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp.paratuberculosis
    Article Snippet: .. After 6 days, collected culture supernatants were assayed for IFN-γ and TNF-α by Bovine IFN-γ ELISA Development Kit (Mabtech, Nacka Strand, Sweden) and Bovine TNF alpha Do-It Yourself ELISA (Kingfisher Biotech, St. Paul, MN, USA), respectively. ..

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    Article Snippet: .. The spent media collected after cell incubation was analyzed for concentrations of TNF-α using a bovine TNF-α commercial kit (VetSet Elisa Development Kit; Kingfisher Biotech Inc., St. Paul, MN) following the manufacturer's protocol with some modifications. ..

    Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease
    Article Snippet: .. To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions. ..

    Article Title: Cytokine and Haptoglobin Profiles From Shipping Through Sickness and Recovery in Metaphylaxis- or Un-Treated Cattle
    Article Snippet: .. This multiplex assay was developed in our lab using “Do-It-Yourself” ELISA kits purchased from Kingfisher Biotech, Inc. (St. Paul, MN; DIY0675B-003, DIY0670B-003, DIY1111B-003). ..

    Article Title: The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis
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    Multiplex Assay:

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  • 94
    Kingfisher Biotech bovine tnf α do it yourself elisa
    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and <t>TNF-α</t> ( e ) concentrations in culture supernatants were determined by <t>ELISA.</t> ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.
    Bovine Tnf α Do It Yourself Elisa, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine tnf α do it yourself elisa/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine tnf α do it yourself elisa - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Journal: Scientific Reports

    Article Title: Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression

    doi: 10.1038/s41598-020-80251-y

    Figure Lengend Snippet: Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Article Snippet: ELISA IFN-γ and TNF-α concentrations in culture supernatants collected from PBMC cultures were determined by Bovine IFN-γ ELISA Development Kit (Mabtech, Nacka Strand, Sweden) and Bovine TNF-α Do-It-Yourself ELISA (Kingfisher Biotech), respectively, according to the manufacturers’ instructions.

    Techniques: Inhibition, Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.

    Journal: Infection and Immunity

    Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

    doi: 10.1128/IAI.00910-17

    Figure Lengend Snippet: Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.

    Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

    Techniques: Functional Assay, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).

    Journal: Infection and Immunity

    Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

    doi: 10.1128/IAI.00910-17

    Figure Lengend Snippet: Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).

    Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

    Techniques: Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing

    Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.

    Journal: Infection and Immunity

    Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

    doi: 10.1128/IAI.00910-17

    Figure Lengend Snippet: Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.

    Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

    Techniques: Activation Assay, Infection, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay