bovine tnf α do it yourself elisa  (Kingfisher Biotech)


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    Kingfisher Biotech bovine tnf α do it yourself elisa
    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and <t>TNF-α</t> ( e ) concentrations in culture supernatants were determined by <t>ELISA.</t> ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.
    Bovine Tnf α Do It Yourself Elisa, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine tnf α do it yourself elisa/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine tnf α do it yourself elisa - by Bioz Stars, 2022-05
    93/100 stars

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    1) Product Images from "Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression"

    Article Title: Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-80251-y

    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.
    Figure Legend Snippet: Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Techniques Used: Inhibition, Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Concentration Assay

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    Kingfisher Biotech bovine tnf α do it yourself elisa
    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and <t>TNF-α</t> ( e ) concentrations in culture supernatants were determined by <t>ELISA.</t> ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.
    Bovine Tnf α Do It Yourself Elisa, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine tnf α do it yourself elisa/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine tnf α do it yourself elisa - by Bioz Stars, 2022-05
    93/100 stars
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    Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Journal: Scientific Reports

    Article Title: Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression

    doi: 10.1038/s41598-020-80251-y

    Figure Lengend Snippet: Inhibition of Th1 responses by Rm-saliva. ( a – e ) PBMCs were cultured with Rm-saliva in the presence of anti-CD3 and anti-CD28 mAbs. ( a ) Gating strategy for CD69 expression. ( b , c ) CD69 expression in CD4 + ( b ) and CD8 + ( c ) cells was measured by flow cytometry. ( d , e ) IFN-γ ( d ) and TNF-α ( e ) concentrations in culture supernatants were determined by ELISA. ( f ) PBMCs were cultured with anti-PD-L1 Ab (Boch4G12) in the presence of Rm-saliva. Bovine IgG was used as a control Ab. PBMCs were stimulated by anti-CD3 and anti-CD28 mAbs. IFN-γ concentration was determined by ELISA. ( a – f ) Statistical difference was identified by the Wilcoxon signed-rank test.

    Article Snippet: ELISA IFN-γ and TNF-α concentrations in culture supernatants collected from PBMC cultures were determined by Bovine IFN-γ ELISA Development Kit (Mabtech, Nacka Strand, Sweden) and Bovine TNF-α Do-It-Yourself ELISA (Kingfisher Biotech), respectively, according to the manufacturers’ instructions.

    Techniques: Inhibition, Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Concentration Assay