elisas  (Kingfisher Biotech)


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    Name:
    Bovine IL 17A Do It Yourself ELISA
    Description:

    Catalog Number:
    DIY0673B-003
    Price:
    700.0
    Quantity:
    1 Set
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    Kingfisher Biotech elisas

    https://www.bioz.com/result/elisas/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisas - by Bioz Stars, 2021-09
    94/100 stars

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    Centrifugation:

    Article Title: Calves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively
    Article Snippet: .. Then, after a centrifugation at 1000× g for 5 min at 4 °C, the supernatant was collected and stored at –80 °C for analysis of IFN-γ and IL-17A production using commercial bovine IFN-γ ELISA kit (#EBC101g, NeoBioscience, Beijing, China) and bovine IL-17A ELISA kit (#VS0284B-002, Kingfisher, St. Paul, MN, USA), respectively, according to the manufacturers’ instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Calves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively
    Article Snippet: .. Then, after a centrifugation at 1000× g for 5 min at 4 °C, the supernatant was collected and stored at –80 °C for analysis of IFN-γ and IL-17A production using commercial bovine IFN-γ ELISA kit (#EBC101g, NeoBioscience, Beijing, China) and bovine IL-17A ELISA kit (#VS0284B-002, Kingfisher, St. Paul, MN, USA), respectively, according to the manufacturers’ instructions. ..

    Article Title: Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
    Article Snippet: .. The protein level of interleukin ( IL)-1β (ESS0027: Invitrogen, Carlsbad, CA, USA), IL-6 (ESS0029: Invitrogen), IL-8 (D8000C: R & D Systems, Minneapolis, MN, USA), IL-12 (OKEH03774: AVIVA SYSTEMS SCIENCE, San Diego, CA, USA), IL-17 (VS0284B-002: Kingfisher Biotech, Saint Paul, MN, USA), and interferon (IFN)-γ (ESS0026B: Invitrogen) in the SF was determined using an ELISA kit. ..

    Article Title: Vitamin A deficiency impairs the immune response to intranasal vaccination and RSV infection in neonatal calves
    Article Snippet: .. ELISAs Bovine IL-17A and IFNγ VetSet ELISA Development kits were purchased from Kingfisher Biotech, Inc and performed according to manufacturer’s instructions. ..

    Article Title: Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis
    Article Snippet: .. Candidate biomarkers for bTB were validated using a bovine IL-8 ELISA kit (Mabtech Inc., Cincinnati, OH, USA), a bovine SAA1 ELISA kit (Aviva Systems Biology, San Diego, CA, USA), a bovine CRP ELISA kit (Aviva Systems Biology, San Diego, CA, USA), a bovine transferrin ELISA kit (Aviva Systems Biology, San Diego, CA, USA), a bovine IP-10 ELISA VetSet kit (Kingfisher Biotech Inc., Saint Paul, MN, USA), and a bovine IL-17A ELISA VetSet kit (Kingfisher Biotech Inc., Saint Paul, MN, USA). ..

    Article Title: Defining immune correlates during latent and active chlamydial infection in sheep
    Article Snippet: .. IL-17A was assayed and quantified using the bovine IL-17A ELISA kit (Kingfisher Biotech, Minneapolis, MN, USA) with rbov IL-17A standards, according to the manufacturer’s instructions; the kit cross-reactivity for ovine IL-17A has previously been reported [ ]. ..

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  • 94
    Kingfisher Biotech bovine il 17a
    Antigen-specific whole blood assay. ( A) Time-course of <t>IL-17A</t> and IFN-γ production by whole blood cultured with OVA. The blood of the 10 responder cows (taken 45 days after the first immunization) was cultured in the presence of OVA (10 μg/mL) for 1 to 4 days in 96-well microplates. For each cow triplicate wells were used for each incubation time. Results are median values and quartiles (Q1; Q3). (B) The effect of magnetic depletion of CD4+ cells on the production of IL-17A and IFN-γ in the antigen-specific whole blood assay. Results obtained by stimulating blood samples from responder cows 15 days after the booster immunization are expressed as the percentage of the production by CD4+-depleted PBMC relative to cytokine production by un processed PBMC (100%). The viability of cells was not altered by the magnetic cell separation.
    Bovine Il 17a, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine il 17a/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine il 17a - by Bioz Stars, 2021-09
    94/100 stars
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    Antigen-specific whole blood assay. ( A) Time-course of IL-17A and IFN-γ production by whole blood cultured with OVA. The blood of the 10 responder cows (taken 45 days after the first immunization) was cultured in the presence of OVA (10 μg/mL) for 1 to 4 days in 96-well microplates. For each cow triplicate wells were used for each incubation time. Results are median values and quartiles (Q1; Q3). (B) The effect of magnetic depletion of CD4+ cells on the production of IL-17A and IFN-γ in the antigen-specific whole blood assay. Results obtained by stimulating blood samples from responder cows 15 days after the booster immunization are expressed as the percentage of the production by CD4+-depleted PBMC relative to cytokine production by un processed PBMC (100%). The viability of cells was not altered by the magnetic cell separation.

    Journal: PLoS ONE

    Article Title: Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes

    doi: 10.1371/journal.pone.0137755

    Figure Lengend Snippet: Antigen-specific whole blood assay. ( A) Time-course of IL-17A and IFN-γ production by whole blood cultured with OVA. The blood of the 10 responder cows (taken 45 days after the first immunization) was cultured in the presence of OVA (10 μg/mL) for 1 to 4 days in 96-well microplates. For each cow triplicate wells were used for each incubation time. Results are median values and quartiles (Q1; Q3). (B) The effect of magnetic depletion of CD4+ cells on the production of IL-17A and IFN-γ in the antigen-specific whole blood assay. Results obtained by stimulating blood samples from responder cows 15 days after the booster immunization are expressed as the percentage of the production by CD4+-depleted PBMC relative to cytokine production by un processed PBMC (100%). The viability of cells was not altered by the magnetic cell separation.

    Article Snippet: Cells were then labeled to reveal intracellular IL-17A with either rabbit antiserum to bovine IL-17A (Kingfisher Biotech) followed by RPE-conjugated anti-rabbit antibody, or PE-conjugated mouse monoclonal antibody to human IL-17A (eBioscience).

    Techniques: Whole Blood Assay, Cell Culture, Incubation, Magnetic Cell Separation

    Persistence of reactivity to ovalbumin. Concentrations of IL-17A and IFN-γ yielded by the whole blood assay performed at different times after immunization with ovalbumin. Results are the median values (and interquartiles) from the 8 responder cows still available 10 months post-immunization.

    Journal: PLoS ONE

    Article Title: Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes

    doi: 10.1371/journal.pone.0137755

    Figure Lengend Snippet: Persistence of reactivity to ovalbumin. Concentrations of IL-17A and IFN-γ yielded by the whole blood assay performed at different times after immunization with ovalbumin. Results are the median values (and interquartiles) from the 8 responder cows still available 10 months post-immunization.

    Article Snippet: Cells were then labeled to reveal intracellular IL-17A with either rabbit antiserum to bovine IL-17A (Kingfisher Biotech) followed by RPE-conjugated anti-rabbit antibody, or PE-conjugated mouse monoclonal antibody to human IL-17A (eBioscience).

    Techniques: Whole Blood Assay

    Intracellular expression of IL-17A and IFN-γ by CD4+ T lymphocytes. PBMC were isolated one month after ovalbumin booster injection, stimulated in vitro with ovalbumin for 3 days, rested for 2 days and finally stimulated with PMA/ionomycin for 5 h with Brefeldin A for the last 3 hours. Cells were labeled for surface CD4 and intracellular IL-17A and IFN-γ. The numbers in the plots indicate the percentages of labeled cells in comparison to the isotype control. (A) Production of viable CD4+ T lymphoblasts after culture of PBMC with (OVA) or without (NS) ovalbumin. Left panels depict PBMC from a responder cow, right panels PBMC from a low-responder. (B) PBMC from two responder cows (R1 R2) were labeled for surface CD4 and intracellular IL-17A or IFN-γ, showing CD4+ and CD4- IL-17A- and IFN-γ-producing cells. (C) labeling of CD4+ cells with anti-IL-17A and anti-IFN-γ antibodies, showing single-producing and double-producing cells. D) Double labeling of CD4+ cells from two low-responders (R3 and R4). Percentages of labeled cells are indicated in the quadrants. Results are from a representative experiment.

    Journal: PLoS ONE

    Article Title: Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes

    doi: 10.1371/journal.pone.0137755

    Figure Lengend Snippet: Intracellular expression of IL-17A and IFN-γ by CD4+ T lymphocytes. PBMC were isolated one month after ovalbumin booster injection, stimulated in vitro with ovalbumin for 3 days, rested for 2 days and finally stimulated with PMA/ionomycin for 5 h with Brefeldin A for the last 3 hours. Cells were labeled for surface CD4 and intracellular IL-17A and IFN-γ. The numbers in the plots indicate the percentages of labeled cells in comparison to the isotype control. (A) Production of viable CD4+ T lymphoblasts after culture of PBMC with (OVA) or without (NS) ovalbumin. Left panels depict PBMC from a responder cow, right panels PBMC from a low-responder. (B) PBMC from two responder cows (R1 R2) were labeled for surface CD4 and intracellular IL-17A or IFN-γ, showing CD4+ and CD4- IL-17A- and IFN-γ-producing cells. (C) labeling of CD4+ cells with anti-IL-17A and anti-IFN-γ antibodies, showing single-producing and double-producing cells. D) Double labeling of CD4+ cells from two low-responders (R3 and R4). Percentages of labeled cells are indicated in the quadrants. Results are from a representative experiment.

    Article Snippet: Cells were then labeled to reveal intracellular IL-17A with either rabbit antiserum to bovine IL-17A (Kingfisher Biotech) followed by RPE-conjugated anti-rabbit antibody, or PE-conjugated mouse monoclonal antibody to human IL-17A (eBioscience).

    Techniques: Expressing, Isolation, Injection, In Vitro, Labeling

    Concentrations of cytokines in milk samples of the 10 responder cows. Time-course of concentration variation (median and interquartiles) of CXCL8 (A), IL-17A (B) and IFN- γ (C) in the milk of quarters infused with ovalbumin at 0 hpi.

    Journal: PLoS ONE

    Article Title: Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes

    doi: 10.1371/journal.pone.0137755

    Figure Lengend Snippet: Concentrations of cytokines in milk samples of the 10 responder cows. Time-course of concentration variation (median and interquartiles) of CXCL8 (A), IL-17A (B) and IFN- γ (C) in the milk of quarters infused with ovalbumin at 0 hpi.

    Article Snippet: Cells were then labeled to reveal intracellular IL-17A with either rabbit antiserum to bovine IL-17A (Kingfisher Biotech) followed by RPE-conjugated anti-rabbit antibody, or PE-conjugated mouse monoclonal antibody to human IL-17A (eBioscience).

    Techniques: Concentration Assay

    Time-course of the IL-17A and IFN-γ production in the antigen-specific whole blood assay following immunization and correlation with Peak SCC. Concentrations of IL-17A (A) or IFN-γ (B) after 3 days of culture with ovalbumin of blood samples taken before and after immunization at days 0 and 30 (median values and interquartiles) distinguishing the antigen-specific responses of responders and low-responder cows to the intramammary antigenic challenge. (B and C) Correlations (Spearman’s rank test) between peak SCC and IL-17A concentrations or IFN-γ concentrations yielded by the whole blood assay performed 45 days after the first immunization.

    Journal: PLoS ONE

    Article Title: Antigen-Specific Mammary Inflammation Depends on the Production of IL-17A and IFN-γ by Bovine CD4+ T Lymphocytes

    doi: 10.1371/journal.pone.0137755

    Figure Lengend Snippet: Time-course of the IL-17A and IFN-γ production in the antigen-specific whole blood assay following immunization and correlation with Peak SCC. Concentrations of IL-17A (A) or IFN-γ (B) after 3 days of culture with ovalbumin of blood samples taken before and after immunization at days 0 and 30 (median values and interquartiles) distinguishing the antigen-specific responses of responders and low-responder cows to the intramammary antigenic challenge. (B and C) Correlations (Spearman’s rank test) between peak SCC and IL-17A concentrations or IFN-γ concentrations yielded by the whole blood assay performed 45 days after the first immunization.

    Article Snippet: Cells were then labeled to reveal intracellular IL-17A with either rabbit antiserum to bovine IL-17A (Kingfisher Biotech) followed by RPE-conjugated anti-rabbit antibody, or PE-conjugated mouse monoclonal antibody to human IL-17A (eBioscience).

    Techniques: Whole Blood Assay

    Enhanced BRSV-specific T cell responses in the peripheral blood and BAL of BRSV-F/G nanovaccine-administered calves. PBMC were collected on day 6 post-challenge, labeled with Cell Trace Violet and stimulated for 6 days with 5 μg/mL of the recombinant BRSV F and G proteins, or 0.01 MOI of BRSV strain 375. Pokeweed Mitogen was used at a concentration of 1 μg/mL as a positive control. Mock stimulated samples (negative control wells) were cultured with cRPMI and were used to correct for background proliferation. ( A ) After 6 days, antigen-specific CD4 T cell proliferation was assessed by flow cytometry, as measured by dilution of the Cell Trace Violet dye. Background levels of proliferation were subtracted and results are presented as change over mock. ( B ) Stimulated cell culture supernatants were collected after 6 days and concentrations of IFNγ (left panel) and IL-17A (right panel) were measured by commercial sandwich ELISAs. ( C ) BALs were performed on day 7 post-challenge. Cells were enumerated and stimulated for 6 days with recombinant BRSV F protein, G protein or BRSV as in A. After 6 days, cell culture supernatants were collected and concentrations of IFNγ (left panel) and IL-17A (right panel) were measured by commercial sandwich ELISAs. Results represent n = 6 animals/group. Data represent means ± SEM. *p

    Journal: Scientific Reports

    Article Title: Efficacy of mucosal polyanhydride nanovaccine against respiratory syncytial virus infection in the neonatal calf

    doi: 10.1038/s41598-018-21292-2

    Figure Lengend Snippet: Enhanced BRSV-specific T cell responses in the peripheral blood and BAL of BRSV-F/G nanovaccine-administered calves. PBMC were collected on day 6 post-challenge, labeled with Cell Trace Violet and stimulated for 6 days with 5 μg/mL of the recombinant BRSV F and G proteins, or 0.01 MOI of BRSV strain 375. Pokeweed Mitogen was used at a concentration of 1 μg/mL as a positive control. Mock stimulated samples (negative control wells) were cultured with cRPMI and were used to correct for background proliferation. ( A ) After 6 days, antigen-specific CD4 T cell proliferation was assessed by flow cytometry, as measured by dilution of the Cell Trace Violet dye. Background levels of proliferation were subtracted and results are presented as change over mock. ( B ) Stimulated cell culture supernatants were collected after 6 days and concentrations of IFNγ (left panel) and IL-17A (right panel) were measured by commercial sandwich ELISAs. ( C ) BALs were performed on day 7 post-challenge. Cells were enumerated and stimulated for 6 days with recombinant BRSV F protein, G protein or BRSV as in A. After 6 days, cell culture supernatants were collected and concentrations of IFNγ (left panel) and IL-17A (right panel) were measured by commercial sandwich ELISAs. Results represent n = 6 animals/group. Data represent means ± SEM. *p

    Article Snippet: Bovine IL-17A, IFNγ, IL-6, IL-1β and TNFα VetSet ELISA Development kits were purchased from Kingfisher Biotech, Inc.

    Techniques: Labeling, Recombinant, Concentration Assay, Positive Control, Negative Control, Cell Culture, Flow Cytometry, Cytometry

    Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Analysis by immunohistochemistry of representative sections of mammary tissue of ovalbumin-infused glands. A) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue (cow #4019) to antibody against N-terminal peptide of bovine IL-17A; B) Immunoreactivity of the mammary tissue of another cow (#1039) to the N-term antibody; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #4019); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase; E, F) Immunoreactivity of the epithelial lining the alveoli and of cells in the connective tissue of cows #4019 and 1039 to the abcam antibody; G-H) Immunoreactivity of mammary tissue of cows #4019 and 1039 to the to the C-term and antibody. Scale bars indicate 25 µm.

    Article Snippet: All these cell types are known to be recruited during mastitis in the bovine MG, but it remains to document which leukocytes are able to produce IL-17A in the bovine species.

    Techniques: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 µm.

    Article Snippet: All these cell types are known to be recruited during mastitis in the bovine MG, but it remains to document which leukocytes are able to produce IL-17A in the bovine species.

    Techniques: Immunohistochemistry, Inhibition, Labeling, Negative Control

    Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Journal: PLoS ONE

    Article Title: T Helper 17-Associated Cytokines Are Produced during Antigen-Specific Inflammation in the Mammary Gland

    doi: 10.1371/journal.pone.0063471

    Figure Lengend Snippet: Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 µg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1β, IL-6, IFN-γ, IL-17A (p

    Article Snippet: All these cell types are known to be recruited during mastitis in the bovine MG, but it remains to document which leukocytes are able to produce IL-17A in the bovine species.

    Techniques: Enzyme-linked Immunosorbent Assay