anti bovine cxcl10 antibody  (Kingfisher Biotech)


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    Kingfisher Biotech anti bovine cxcl10 antibody
    Antigen-specific <t>IP-10</t> release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.
    Anti Bovine Cxcl10 Antibody, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bovine cxcl10 antibody/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bovine cxcl10 antibody - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle"

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0155440

    Antigen-specific IP-10 release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.
    Figure Legend Snippet: Antigen-specific IP-10 release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.

    Techniques Used: Incubation

    Correlation between IFN-γ and IP-10 release. Whole blood from non-reactor and reactor cattle was stimulated with either M . bovis PPD or M . avium PPD for 24 h at 37°C. The release of IFN-γ and IP-10 was highly correlated in these samples (r = 0.65; p
    Figure Legend Snippet: Correlation between IFN-γ and IP-10 release. Whole blood from non-reactor and reactor cattle was stimulated with either M . bovis PPD or M . avium PPD for 24 h at 37°C. The release of IFN-γ and IP-10 was highly correlated in these samples (r = 0.65; p

    Techniques Used:

    Spontaneous IP-10 release in whole blood from reactor cattle. Whole blood from cattle (n = 12) was incubated with phosphate buffered saline for 24 and 48 h. IP-10 release showed a significant increase over time (*
    Figure Legend Snippet: Spontaneous IP-10 release in whole blood from reactor cattle. Whole blood from cattle (n = 12) was incubated with phosphate buffered saline for 24 and 48 h. IP-10 release showed a significant increase over time (*

    Techniques Used: Incubation

    IP-10 release in response to antigenic stimulation in reactor and non-reactor cattle. Whole blood from non-reactor (n = 16) and reactor (n = 12) cattle was incubated with M . avium PPD (PPDa) and M . bovis PPD (PPDb) for 24 h at 37°C. For reactor cattle, IP-10 release in response to PPDb was significantly greater than that in response to PPDa. Median values are shown (*, p
    Figure Legend Snippet: IP-10 release in response to antigenic stimulation in reactor and non-reactor cattle. Whole blood from non-reactor (n = 16) and reactor (n = 12) cattle was incubated with M . avium PPD (PPDa) and M . bovis PPD (PPDb) for 24 h at 37°C. For reactor cattle, IP-10 release in response to PPDb was significantly greater than that in response to PPDa. Median values are shown (*, p

    Techniques Used: Incubation

    IP-10 release in response to antigenic stimulation after (A) 24 h and (B) 48 h. Whole blood from bTB-positive cattle (n = 12) was incubated at 37°C with saline (PBS), M . avium PPD (PPDa), M . bovis PPD (PPDb) and ESAT-6/CFP-10 peptides (EC). IP-10 release in whole blood in response to PPDb and EC was significantly greater than that in blood co-incubated with PBS and significantly greater in response to PPDb than in response to PPDa. Median values and the differential IP-10 responses to PPDa and PPDb (ΔPPD) and PBS and EC (ΔEC) are shown (*, p
    Figure Legend Snippet: IP-10 release in response to antigenic stimulation after (A) 24 h and (B) 48 h. Whole blood from bTB-positive cattle (n = 12) was incubated at 37°C with saline (PBS), M . avium PPD (PPDa), M . bovis PPD (PPDb) and ESAT-6/CFP-10 peptides (EC). IP-10 release in whole blood in response to PPDb and EC was significantly greater than that in blood co-incubated with PBS and significantly greater in response to PPDb than in response to PPDa. Median values and the differential IP-10 responses to PPDa and PPDb (ΔPPD) and PBS and EC (ΔEC) are shown (*, p

    Techniques Used: Incubation

    2) Product Images from "Cell-Mediated Immunological Biomarkers and Their Diagnostic Application in Livestock and Wildlife Infected With Mycobacterium bovis"

    Article Title: Cell-Mediated Immunological Biomarkers and Their Diagnostic Application in Livestock and Wildlife Infected With Mycobacterium bovis

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.639605

    Overview of hypothesized interactions of selected cytokines/chemokines identified as bTB biomarkers in domestic and wild animal species. CXCL10: IP-10. Created in BioRender.com .
    Figure Legend Snippet: Overview of hypothesized interactions of selected cytokines/chemokines identified as bTB biomarkers in domestic and wild animal species. CXCL10: IP-10. Created in BioRender.com .

    Techniques Used:

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    • anti bovine cxcl10 antibody  (Kingfisher Biotech)
    93
    Kingfisher Biotech anti bovine cxcl10 antibody
    Antigen-specific <t>IP-10</t> release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.
    Anti Bovine Cxcl10 Antibody, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bovine cxcl10 antibody/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bovine cxcl10 antibody - by Bioz Stars, 2022-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Antigen-specific IP-10 release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.

    Journal: PLoS ONE

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    doi: 10.1371/journal.pone.0155440

    Figure Lengend Snippet: Antigen-specific IP-10 release distinguishes between non-reactor and reactor cattle. Whole blood from non-reactor (n = 36) and reactor cattle (n = 24) was incubated with M . bovis PPD and M . avium PPD for 24 h at 37°C. The difference in IP-10 release in these samples (ΔPPD) was used to calculate, by ROC analysis, an optimal cut off value of 0.11 (dotted line). This distinguished between these groups with a sensitivity of 100% and a specificity of 97%.

    Article Snippet: Anti-bovine CXCL10 antibody (Kingfisher Biotech Inc., St Paul, MN, USA) in PBS (1 μg/ml; 100 μl/well) was incubated overnight at 4°C in 96-well Nunc Maxisorb microtitre plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Incubation

    Correlation between IFN-γ and IP-10 release. Whole blood from non-reactor and reactor cattle was stimulated with either M . bovis PPD or M . avium PPD for 24 h at 37°C. The release of IFN-γ and IP-10 was highly correlated in these samples (r = 0.65; p

    Journal: PLoS ONE

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    doi: 10.1371/journal.pone.0155440

    Figure Lengend Snippet: Correlation between IFN-γ and IP-10 release. Whole blood from non-reactor and reactor cattle was stimulated with either M . bovis PPD or M . avium PPD for 24 h at 37°C. The release of IFN-γ and IP-10 was highly correlated in these samples (r = 0.65; p

    Article Snippet: Anti-bovine CXCL10 antibody (Kingfisher Biotech Inc., St Paul, MN, USA) in PBS (1 μg/ml; 100 μl/well) was incubated overnight at 4°C in 96-well Nunc Maxisorb microtitre plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques:

    Spontaneous IP-10 release in whole blood from reactor cattle. Whole blood from cattle (n = 12) was incubated with phosphate buffered saline for 24 and 48 h. IP-10 release showed a significant increase over time (*

    Journal: PLoS ONE

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    doi: 10.1371/journal.pone.0155440

    Figure Lengend Snippet: Spontaneous IP-10 release in whole blood from reactor cattle. Whole blood from cattle (n = 12) was incubated with phosphate buffered saline for 24 and 48 h. IP-10 release showed a significant increase over time (*

    Article Snippet: Anti-bovine CXCL10 antibody (Kingfisher Biotech Inc., St Paul, MN, USA) in PBS (1 μg/ml; 100 μl/well) was incubated overnight at 4°C in 96-well Nunc Maxisorb microtitre plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Incubation

    IP-10 release in response to antigenic stimulation in reactor and non-reactor cattle. Whole blood from non-reactor (n = 16) and reactor (n = 12) cattle was incubated with M . avium PPD (PPDa) and M . bovis PPD (PPDb) for 24 h at 37°C. For reactor cattle, IP-10 release in response to PPDb was significantly greater than that in response to PPDa. Median values are shown (*, p

    Journal: PLoS ONE

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    doi: 10.1371/journal.pone.0155440

    Figure Lengend Snippet: IP-10 release in response to antigenic stimulation in reactor and non-reactor cattle. Whole blood from non-reactor (n = 16) and reactor (n = 12) cattle was incubated with M . avium PPD (PPDa) and M . bovis PPD (PPDb) for 24 h at 37°C. For reactor cattle, IP-10 release in response to PPDb was significantly greater than that in response to PPDa. Median values are shown (*, p

    Article Snippet: Anti-bovine CXCL10 antibody (Kingfisher Biotech Inc., St Paul, MN, USA) in PBS (1 μg/ml; 100 μl/well) was incubated overnight at 4°C in 96-well Nunc Maxisorb microtitre plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Incubation

    IP-10 release in response to antigenic stimulation after (A) 24 h and (B) 48 h. Whole blood from bTB-positive cattle (n = 12) was incubated at 37°C with saline (PBS), M . avium PPD (PPDa), M . bovis PPD (PPDb) and ESAT-6/CFP-10 peptides (EC). IP-10 release in whole blood in response to PPDb and EC was significantly greater than that in blood co-incubated with PBS and significantly greater in response to PPDb than in response to PPDa. Median values and the differential IP-10 responses to PPDa and PPDb (ΔPPD) and PBS and EC (ΔEC) are shown (*, p

    Journal: PLoS ONE

    Article Title: Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

    doi: 10.1371/journal.pone.0155440

    Figure Lengend Snippet: IP-10 release in response to antigenic stimulation after (A) 24 h and (B) 48 h. Whole blood from bTB-positive cattle (n = 12) was incubated at 37°C with saline (PBS), M . avium PPD (PPDa), M . bovis PPD (PPDb) and ESAT-6/CFP-10 peptides (EC). IP-10 release in whole blood in response to PPDb and EC was significantly greater than that in blood co-incubated with PBS and significantly greater in response to PPDb than in response to PPDa. Median values and the differential IP-10 responses to PPDa and PPDb (ΔPPD) and PBS and EC (ΔEC) are shown (*, p

    Article Snippet: Anti-bovine CXCL10 antibody (Kingfisher Biotech Inc., St Paul, MN, USA) in PBS (1 μg/ml; 100 μl/well) was incubated overnight at 4°C in 96-well Nunc Maxisorb microtitre plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Incubation

    Overview of hypothesized interactions of selected cytokines/chemokines identified as bTB biomarkers in domestic and wild animal species. CXCL10: IP-10. Created in BioRender.com .

    Journal: Frontiers in Immunology

    Article Title: Cell-Mediated Immunological Biomarkers and Their Diagnostic Application in Livestock and Wildlife Infected With Mycobacterium bovis

    doi: 10.3389/fimmu.2021.639605

    Figure Lengend Snippet: Overview of hypothesized interactions of selected cytokines/chemokines identified as bTB biomarkers in domestic and wild animal species. CXCL10: IP-10. Created in BioRender.com .

    Article Snippet: Using a Kingfisher Biotech (St Paul, MN, United States) bovine IP-10 ELISA and QFT whole blood stimulation, a significantly higher antigen-specific IP-10 response was able to distinguish M. bovis- infected from culture negative warthogs ( ).

    Techniques: