pcmv3 untagged negative control vector  (Sino Biological)


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    Name:
    GFP cDNA ORF Clone Aequorea victoria untagged
    Description:
    Full length Clone DNA of green fluorescent protein
    Catalog Number:
    AG13105-UT
    Price:
    None
    Category:
    Gene
    Product Aliases:
    GFP cDNA ORF Clone Aequorea victoria
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    Structured Review

    Sino Biological pcmv3 untagged negative control vector
    Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either <t>pCMV3-CD56</t> plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p
    Full length Clone DNA of green fluorescent protein
    https://www.bioz.com/result/pcmv3 untagged negative control vector/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 untagged negative control vector - by Bioz Stars, 2021-05
    80/100 stars

    Images

    1) Product Images from "CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse"

    Article Title: CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-45377-8

    Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either pCMV3-CD56 plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p
    Figure Legend Snippet: Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either pCMV3-CD56 plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p

    Techniques Used: Expressing, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Flow Cytometry, Labeling, Immunofluorescence, Microscopy, Cytotoxicity Assay, Fluorescence

    Related Articles

    Plasmid Preparation:

    Article Title: Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling.
    Article Snippet: For inhibition of the β-catenin pathway, 10 μM XAV-939 (MedChem Express, NJ, USA) was added into the culture 4 h prior to addition of 50 ng/ml EGF or 4 h after transfection. .. TransfectionThe plasmid encoding the open reading frame of murine EGF, pCMV3-mEGF (# MG50482-UT), and the control vector pCMV3-untagged (#CV011) were purchased from Sino Biological Inc. ORS cells were plated in 6-well plates at 3 × 105 cells/well or in 96-well plates at 4,000 cells/well, and cultured for 24 h. The cells were incubated with serum-free DMEM for 1 h, followed by transfection with the EGF overexpression plasmids (EGF O/E) or the control vector using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: n-Butylidenephthalide exhibits protection against neurotoxicity through regulation of tryptophan 2, 3 dioxygenase in spinocerebellar ataxia type 3.
    Article Snippet: Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner. .. Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner. .. Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner.

    Article Title: CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse
    Article Snippet: .. Transient transfectionCells were grown to 70–80% confluence and transfected with pCMV3-NCAM1 plasmid (2.5 μg; Cat # HG10673-UT Sinobiological, Wayne, PA, USA) or empty pCMV3 plasmid (2.5 μg; Cat # CV011 Sinobiological) using Lipofectamine 3000 transfection kit (Cat # L3000-015, Invitrogen, ThermoFisher Scientific) according to the manufacturer’s protocol. .. At 48 hours post-transfection, cells were collected for assessment of transfection efficiency and subsequent analyses.

    Article Title: Human Organic Anion Transporting Polypeptide 1B3 Applied as an MRI-Based Reporter Gene
    Article Snippet: The cells were maintained in high-glucose Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Hyclone, Logan, UT, USA), 1% penicillin, and 1% streptomycin (Hyclone). .. HOATP1B3 cDNA in the pCMV3 vector (HG13013-UT, Sino biological Inc., Beijing, China) and the control vector CV011 (Sino biological Inc.) were transfected into HEK293 cells using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA). .. To select transfected clones, 100 µg/mL hygromycin B (Invitrogen) was added to the cell cultures, and isolated colonies were picked using microscopy and grown separately.

    Article Title: Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing
    Article Snippet: The production of the siRNA for USP7 knockdown was done by GenePharma, Shanghai, China. .. For plasmids, full-length expression cDNA of PD-L1 (Flag-PD-L1 , HG10084-NF), HA-USP7 (HG11681-CY) and pCMV3-untagged negative control vector were purchased from Sino Biological Inc. (Beijing, China). pCDNA-HA-His-Ub was generated by GENEWIZ, Suzhou, China. .. 2.6 Detection of cell surface PD-L1To analyze the cell surface PD-L1, 100 μL staining buffer containing human PE-PD-L1 antibody (catalog557924; BD Biosciences, Franklin Lakes, NJ, USA) was prepared and cells were suspended in it, and then incubated the mixture at room temperature for 30 min.

    Article Title: Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway.
    Article Snippet: The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial.

    Cell Culture:

    Article Title: Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling.
    Article Snippet: For inhibition of the β-catenin pathway, 10 μM XAV-939 (MedChem Express, NJ, USA) was added into the culture 4 h prior to addition of 50 ng/ml EGF or 4 h after transfection. .. TransfectionThe plasmid encoding the open reading frame of murine EGF, pCMV3-mEGF (# MG50482-UT), and the control vector pCMV3-untagged (#CV011) were purchased from Sino Biological Inc. ORS cells were plated in 6-well plates at 3 × 105 cells/well or in 96-well plates at 4,000 cells/well, and cultured for 24 h. The cells were incubated with serum-free DMEM for 1 h, followed by transfection with the EGF overexpression plasmids (EGF O/E) or the control vector using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling.
    Article Snippet: For inhibition of the β-catenin pathway, 10 μM XAV-939 (MedChem Express, NJ, USA) was added into the culture 4 h prior to addition of 50 ng/ml EGF or 4 h after transfection. .. TransfectionThe plasmid encoding the open reading frame of murine EGF, pCMV3-mEGF (# MG50482-UT), and the control vector pCMV3-untagged (#CV011) were purchased from Sino Biological Inc. ORS cells were plated in 6-well plates at 3 × 105 cells/well or in 96-well plates at 4,000 cells/well, and cultured for 24 h. The cells were incubated with serum-free DMEM for 1 h, followed by transfection with the EGF overexpression plasmids (EGF O/E) or the control vector using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Transfection:

    Article Title: Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling.
    Article Snippet: For inhibition of the β-catenin pathway, 10 μM XAV-939 (MedChem Express, NJ, USA) was added into the culture 4 h prior to addition of 50 ng/ml EGF or 4 h after transfection. .. TransfectionThe plasmid encoding the open reading frame of murine EGF, pCMV3-mEGF (# MG50482-UT), and the control vector pCMV3-untagged (#CV011) were purchased from Sino Biological Inc. ORS cells were plated in 6-well plates at 3 × 105 cells/well or in 96-well plates at 4,000 cells/well, and cultured for 24 h. The cells were incubated with serum-free DMEM for 1 h, followed by transfection with the EGF overexpression plasmids (EGF O/E) or the control vector using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse
    Article Snippet: .. Transient transfectionCells were grown to 70–80% confluence and transfected with pCMV3-NCAM1 plasmid (2.5 μg; Cat # HG10673-UT Sinobiological, Wayne, PA, USA) or empty pCMV3 plasmid (2.5 μg; Cat # CV011 Sinobiological) using Lipofectamine 3000 transfection kit (Cat # L3000-015, Invitrogen, ThermoFisher Scientific) according to the manufacturer’s protocol. .. At 48 hours post-transfection, cells were collected for assessment of transfection efficiency and subsequent analyses.

    Article Title: Human Organic Anion Transporting Polypeptide 1B3 Applied as an MRI-Based Reporter Gene
    Article Snippet: The cells were maintained in high-glucose Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Hyclone, Logan, UT, USA), 1% penicillin, and 1% streptomycin (Hyclone). .. HOATP1B3 cDNA in the pCMV3 vector (HG13013-UT, Sino biological Inc., Beijing, China) and the control vector CV011 (Sino biological Inc.) were transfected into HEK293 cells using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA). .. To select transfected clones, 100 µg/mL hygromycin B (Invitrogen) was added to the cell cultures, and isolated colonies were picked using microscopy and grown separately.

    Article Title: Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway.
    Article Snippet: The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial.

    Over Expression:

    Article Title: Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling.
    Article Snippet: For inhibition of the β-catenin pathway, 10 μM XAV-939 (MedChem Express, NJ, USA) was added into the culture 4 h prior to addition of 50 ng/ml EGF or 4 h after transfection. .. TransfectionThe plasmid encoding the open reading frame of murine EGF, pCMV3-mEGF (# MG50482-UT), and the control vector pCMV3-untagged (#CV011) were purchased from Sino Biological Inc. ORS cells were plated in 6-well plates at 3 × 105 cells/well or in 96-well plates at 4,000 cells/well, and cultured for 24 h. The cells were incubated with serum-free DMEM for 1 h, followed by transfection with the EGF overexpression plasmids (EGF O/E) or the control vector using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: n-Butylidenephthalide exhibits protection against neurotoxicity through regulation of tryptophan 2, 3 dioxygenase in spinocerebellar ataxia type 3.
    Article Snippet: Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner. .. Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner. .. Spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) is characterized by the repetition of a CAG codon in the ataxin-3 gene (ATXN3), which leads to the formation of an elongated mutant ATXN3 protein that can neither be denatured nor undergo proteolysis in the normal manner.

    Recombinant:

    Article Title: SARS-CoV-2 mutations acquired in mink reduce antibody-mediated neutralization
    Article Snippet: Serum and plasma samples were pre-screened for neutralizing activity using SARS-2-S WT pseudotypes, as described below. .. Production of recombinant human monoclonal antibodies against SARS-CoV-2 spikeVH and VL sequences of Regeneron antibodies Casirivimab/REGN10933, Imdevimab/REGN10987 and REGN10989 ( ) were cloned in pCMC3-untagged-NCV (SINO Biologics, Cat: CV011) and produced in 293T cells by SINO Biological (Beijing, China). .. The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific, Schwerte, Germany, Cat. no. R790-07) with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific, Cat. no. K9000-01).

    Clone Assay:

    Article Title: SARS-CoV-2 mutations acquired in mink reduce antibody-mediated neutralization
    Article Snippet: Serum and plasma samples were pre-screened for neutralizing activity using SARS-2-S WT pseudotypes, as described below. .. Production of recombinant human monoclonal antibodies against SARS-CoV-2 spikeVH and VL sequences of Regeneron antibodies Casirivimab/REGN10933, Imdevimab/REGN10987 and REGN10989 ( ) were cloned in pCMC3-untagged-NCV (SINO Biologics, Cat: CV011) and produced in 293T cells by SINO Biological (Beijing, China). .. The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific, Schwerte, Germany, Cat. no. R790-07) with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific, Cat. no. K9000-01).

    Produced:

    Article Title: SARS-CoV-2 mutations acquired in mink reduce antibody-mediated neutralization
    Article Snippet: Serum and plasma samples were pre-screened for neutralizing activity using SARS-2-S WT pseudotypes, as described below. .. Production of recombinant human monoclonal antibodies against SARS-CoV-2 spikeVH and VL sequences of Regeneron antibodies Casirivimab/REGN10933, Imdevimab/REGN10987 and REGN10989 ( ) were cloned in pCMC3-untagged-NCV (SINO Biologics, Cat: CV011) and produced in 293T cells by SINO Biological (Beijing, China). .. The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific, Schwerte, Germany, Cat. no. R790-07) with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific, Cat. no. K9000-01).

    Negative Control:

    Article Title: A new inhibitor of glucose-6-phosphate dehydrogenase blocks pentose phosphate pathway and suppresses malignant proliferation and metastasis in vivo
    Article Snippet: DNA content and cell cycle distribution were measured with a FACSARIA III or BD Accuri Cytometer, data were analyzed using Mod-Fit software (Verity Software House, USA). .. p3-G6PD-t1 and negative control pCMV3-untagged-NCV (control) hygroycin-resistant plasmids were purchased from Sino Biological Inc. (Sino Biological, Beijing, China). .. MCF7 cells were stably transfected with Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA USA) according to the manufacturer’s instructions.

    Article Title: Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing
    Article Snippet: The production of the siRNA for USP7 knockdown was done by GenePharma, Shanghai, China. .. For plasmids, full-length expression cDNA of PD-L1 (Flag-PD-L1 , HG10084-NF), HA-USP7 (HG11681-CY) and pCMV3-untagged negative control vector were purchased from Sino Biological Inc. (Beijing, China). pCDNA-HA-His-Ub was generated by GENEWIZ, Suzhou, China. .. 2.6 Detection of cell surface PD-L1To analyze the cell surface PD-L1, 100 μL staining buffer containing human PE-PD-L1 antibody (catalog557924; BD Biosciences, Franklin Lakes, NJ, USA) was prepared and cells were suspended in it, and then incubated the mixture at room temperature for 30 min.

    Expressing:

    Article Title: Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing
    Article Snippet: The production of the siRNA for USP7 knockdown was done by GenePharma, Shanghai, China. .. For plasmids, full-length expression cDNA of PD-L1 (Flag-PD-L1 , HG10084-NF), HA-USP7 (HG11681-CY) and pCMV3-untagged negative control vector were purchased from Sino Biological Inc. (Beijing, China). pCDNA-HA-His-Ub was generated by GENEWIZ, Suzhou, China. .. 2.6 Detection of cell surface PD-L1To analyze the cell surface PD-L1, 100 μL staining buffer containing human PE-PD-L1 antibody (catalog557924; BD Biosciences, Franklin Lakes, NJ, USA) was prepared and cells were suspended in it, and then incubated the mixture at room temperature for 30 min.

    Article Title: Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway.
    Article Snippet: The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial. .. The effect of epidermal growth factor (EGF) on the development and growth of hair follicle is controversial.

    Generated:

    Article Title: Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing
    Article Snippet: The production of the siRNA for USP7 knockdown was done by GenePharma, Shanghai, China. .. For plasmids, full-length expression cDNA of PD-L1 (Flag-PD-L1 , HG10084-NF), HA-USP7 (HG11681-CY) and pCMV3-untagged negative control vector were purchased from Sino Biological Inc. (Beijing, China). pCDNA-HA-His-Ub was generated by GENEWIZ, Suzhou, China. .. 2.6 Detection of cell surface PD-L1To analyze the cell surface PD-L1, 100 μL staining buffer containing human PE-PD-L1 antibody (catalog557924; BD Biosciences, Franklin Lakes, NJ, USA) was prepared and cells were suspended in it, and then incubated the mixture at room temperature for 30 min.

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  • 80
    Sino Biological pcmv3 untagged negative control vector
    Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either <t>pCMV3-CD56</t> plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p
    Pcmv3 Untagged Negative Control Vector, supplied by Sino Biological, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv3 untagged negative control vector/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 untagged negative control vector - by Bioz Stars, 2021-05
    80/100 stars
      Buy from Supplier

    86
    Sino Biological pcmc3 untagged ncv
    Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either <t>pCMV3-CD56</t> plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p
    Pcmc3 Untagged Ncv, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmc3 untagged ncv/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmc3 untagged ncv - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either pCMV3-CD56 plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p

    Journal: Scientific Reports

    Article Title: CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse

    doi: 10.1038/s41598-019-45377-8

    Figure Lengend Snippet: Induction of CD56 expression in breast cancer cells enhances their sensitivity to NK-92-mediated cytotoxicity. CD56-negative MCF-7 and MDA-MB-231 parental cells were transiently transfected with either pCMV3-CD56 plasmid or pCMV3 empty plasmid. After 48 hours, the transfected cells were assessed for CD56 expression by flow cytometry (panel A), for the rate of immune synapse formation and CD56 localization by CFSE-labeling, coculture with NK-92 cells at 1:1 effector-to-target ratio for 2 h and immunofluorescence microscopy (panel B and C) and for their responsiveness to NK-92-mediated cytotoxicity by calcein-AM cytotoxicity assay (panel D). ( A ) The histograms presented indicate the percentage of CD56-positive cells and are representative results from at least five independent experiments. ( B ) Fluorescence images are representative fields from two independent experiments. ( C ) Graphs presenting the mean rates of synapse formation ± SD obtained from two full slides for each condition. ( D ) Results presented are means ± SD for three independent experiments performed each in triplicates. **p

    Article Snippet: Transient transfectionCells were grown to 70–80% confluence and transfected with pCMV3-NCAM1 plasmid (2.5 μg; Cat # HG10673-UT Sinobiological, Wayne, PA, USA) or empty pCMV3 plasmid (2.5 μg; Cat # CV011 Sinobiological) using Lipofectamine 3000 transfection kit (Cat # L3000-015, Invitrogen, ThermoFisher Scientific) according to the manufacturer’s protocol.

    Techniques: Expressing, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Flow Cytometry, Labeling, Immunofluorescence, Microscopy, Cytotoxicity Assay, Fluorescence