qpcr  (ATCC)


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    ATCC qpcr
    Qpcr, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr/product/ATCC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qpcr - by Bioz Stars, 2022-12
    88/100 stars

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    ATCC laboratory s 8e5 cell line 8e5a atcc crl 8993tm
    Standard test of the qPCR kit over several years and of different <t>cell</t> lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> <t>cell-line</t> and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    Laboratory S 8e5 Cell Line 8e5a Atcc Crl 8993tm, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laboratory s 8e5 cell line 8e5a atcc crl 8993tm/product/ATCC
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    laboratory s 8e5 cell line 8e5a atcc crl 8993tm - by Bioz Stars, 2022-12
    94/100 stars
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    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

    Journal: Scientific Reports

    Article Title: Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy

    doi: 10.1038/s41598-022-13581-8

    Figure Lengend Snippet: Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

    Article Snippet: The laboratory’s 8E5 cell line (8E5A)(ATCC CRL-8993TM) was thawed and cultured in RPMI 1640 medium complete with 9% SV F and stored at 37 °C and 5% CO2 .

    Techniques: Real-time Polymerase Chain Reaction, Digital PCR

    Determination of the Optimal scFv for Anti-HIV CAR Therapy (A) Gene structure of second generation LV-derived NIH45-46 CAR containing 5′ and 3′ truncated LTRs, IgG4 linker (L) containing mutations L235E and N297Q, a CD4 transmembrane (TM) domain, 4-1BB costimulatory domain, and CD3ζ domain. A T2A skip separates a truncated EGFR (EGFRt) from the CAR. (B) Transduced T cells with various second-generation CAR constructs were co-cultured with GFP-expressing HEK.GP160s or HEKs for 24 h prior to analyzing CD137 expression by flow cytometry. (C and D) Donor 1 (C) and donor 2 (D) T cells were transduced with various second generation CAR constructs derived from neutralizing antibodies that were cultured with GFP-expressing 8e5 or CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as a control. All samples, except CEM control in donor 2, were run in duplicates.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Conditionally Replicating Vectors Mobilize Chimeric Antigen Receptors against HIV

    doi: 10.1016/j.omtm.2020.09.014

    Figure Lengend Snippet: Determination of the Optimal scFv for Anti-HIV CAR Therapy (A) Gene structure of second generation LV-derived NIH45-46 CAR containing 5′ and 3′ truncated LTRs, IgG4 linker (L) containing mutations L235E and N297Q, a CD4 transmembrane (TM) domain, 4-1BB costimulatory domain, and CD3ζ domain. A T2A skip separates a truncated EGFR (EGFRt) from the CAR. (B) Transduced T cells with various second-generation CAR constructs were co-cultured with GFP-expressing HEK.GP160s or HEKs for 24 h prior to analyzing CD137 expression by flow cytometry. (C and D) Donor 1 (C) and donor 2 (D) T cells were transduced with various second generation CAR constructs derived from neutralizing antibodies that were cultured with GFP-expressing 8e5 or CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as a control. All samples, except CEM control in donor 2, were run in duplicates.

    Article Snippet: Cell Lines Jurkats (ATCC TIB-152), CEM (ATCC CRL-2265), and 8e5 (ATCC CRL-8993) cell lines were obtained from ATCC.

    Techniques: Derivative Assay, Construct, Cell Culture, Expressing, Flow Cytometry, Transduction

    Functionality of crLV-Derived NIH45-46 CAR T Cells (A) Gene structure of second generation crLV-derived NIH45-46 CAR containing 5′ and 3′ full-length LTRs, non-coding regions for gag, pol, and envelope. (B) Transduced T cells with crLV- or LV-derived NIH45-46 were co-cultured with GFP-expressing HEK.GP160s or HEKs for 24 h prior to analyzing CD137 expression by flow cytometry. (C) crLV- and LV-derived NIH45-46 CAR T cells were cultured with GFP-expressing 8e5 or CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as a control. All samples were run in duplicates. (D) The crLV- and LV-derived NIH45-46 CAR T cells were co-cultured with NL4-3 infected or uninfected GFP-expressing CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as control. All samples were run in duplicates.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Conditionally Replicating Vectors Mobilize Chimeric Antigen Receptors against HIV

    doi: 10.1016/j.omtm.2020.09.014

    Figure Lengend Snippet: Functionality of crLV-Derived NIH45-46 CAR T Cells (A) Gene structure of second generation crLV-derived NIH45-46 CAR containing 5′ and 3′ full-length LTRs, non-coding regions for gag, pol, and envelope. (B) Transduced T cells with crLV- or LV-derived NIH45-46 were co-cultured with GFP-expressing HEK.GP160s or HEKs for 24 h prior to analyzing CD137 expression by flow cytometry. (C) crLV- and LV-derived NIH45-46 CAR T cells were cultured with GFP-expressing 8e5 or CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as a control. All samples were run in duplicates. (D) The crLV- and LV-derived NIH45-46 CAR T cells were co-cultured with NL4-3 infected or uninfected GFP-expressing CEM cells for 4 days prior to analyzing cell line survival by flow cytometry. Mock-transduced T cells were used as control. All samples were run in duplicates.

    Article Snippet: Cell Lines Jurkats (ATCC TIB-152), CEM (ATCC CRL-2265), and 8e5 (ATCC CRL-8993) cell lines were obtained from ATCC.

    Techniques: Derivative Assay, Cell Culture, Expressing, Flow Cytometry, Infection