igg control  (Sino Biological)


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  • 91
    Name:
    Normal Goat Control IgG
    Description:

    Catalog Number:
    CR2
    Price:
    None
    Category:
    Control Antibody
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    Structured Review

    Sino Biological igg control
    <t>rWnt2</t> induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or <t>IgG</t> control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    https://www.bioz.com/result/igg control/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg control - by Bioz Stars, 2021-08
    91/100 stars

    Images

    1) Product Images from "Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway"

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

    Journal: American Journal of Cancer Research

    doi:

    rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P
    Figure Legend Snippet: rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    Techniques Used: Translocation Assay, Incubation, Concentration Assay, Western Blot

    2) Product Images from "Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway"

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

    Journal: American Journal of Cancer Research

    doi:

    rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P
    Figure Legend Snippet: rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    Techniques Used: Translocation Assay, Incubation, Concentration Assay, Western Blot

    3) Product Images from "Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway"

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

    Journal: American Journal of Cancer Research

    doi:

    rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P
    Figure Legend Snippet: rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    Techniques Used: Translocation Assay, Incubation, Concentration Assay, Western Blot

    Related Articles

    other:

    Article Title: The Transcription Factor AP4 Promotes Oncogenic Phenotypes and Cisplatin Resistance by Regulating LAPTM4B Expression.
    Article Snippet: Normal goat IgG was the control.

    Translocation Assay:

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. Nevertheless the nuclear translocation of β-catenin could be hardly observed when H460 cells were incubated with IgG control. ..

    Incubation:

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. Nevertheless the nuclear translocation of β-catenin could be hardly observed when H460 cells were incubated with IgG control. ..

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. We incubated wild type H460 cells with rWnt2 or IgG control for 3 hours and then extracted the nuclear and cytoplasmic protein. ..

    Cell Culture:

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. Cells cultured with Wnt2 recombinant protein (rWnt2, Abnova) or IgG control (Sino Biological Inc.) at a concentration of 200 ng/mL were seeded on coverslips for the indicated time points and then fixed in 4% paraformaldehyde at room temperature for 15 min. ..

    Recombinant:

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. Cells cultured with Wnt2 recombinant protein (rWnt2, Abnova) or IgG control (Sino Biological Inc.) at a concentration of 200 ng/mL were seeded on coverslips for the indicated time points and then fixed in 4% paraformaldehyde at room temperature for 15 min. ..

    Concentration Assay:

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway
    Article Snippet: .. Cells cultured with Wnt2 recombinant protein (rWnt2, Abnova) or IgG control (Sino Biological Inc.) at a concentration of 200 ng/mL were seeded on coverslips for the indicated time points and then fixed in 4% paraformaldehyde at room temperature for 15 min. ..

    Immunoprecipitation:

    Article Title: The Transcription Factor AP4 Promotes Oncogenic Phenotypes and Cisplatin Resistance by Regulating LAPTM4B Expression.
    Article Snippet: .. The sheared chromatin was then immunoprecipitated by using goat polyclonal AP-4 antibody (sc-18593X, Santa Cruz Biotechnology) or normal goat IgG (CR2, Sino Biological Inc). ..

    Mouse Assay:

    Article Title: Therapeutic efficacy of a co-blockade of IL-13 and IL-25 on airway inflammation and remodeling in a mouse model of asthma.
    Article Snippet: .. Repeated airway inflammation and unremitting remodeling provoke irreversible pulmonary dysfunction and resistance to current drugs in patients with chronic bronchial asthma. ..

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  • 91
    Sino Biological igg control
    <t>rWnt2</t> induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or <t>IgG</t> control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P
    Igg Control, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg control/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg control - by Bioz Stars, 2021-08
    91/100 stars
      Buy from Supplier

    91
    Sino Biological soluble cr2 cd21
    ELISA competitive experiments showing that CIP inhibits the formation of the <t>C3d–CR2/CD21</t> complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by anti-C3 pAb and HRP-conjugated secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.
    Soluble Cr2 Cd21, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soluble cr2 cd21/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soluble cr2 cd21 - by Bioz Stars, 2021-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    Journal: American Journal of Cancer Research

    Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

    doi:

    Figure Lengend Snippet: rWnt2 induces nuclear translocation of β-catenin and activates Wnt/β-catenin signalling pathway in H460 cells. A: Wild type of H460 cells were incubated with rWnt2 or IgG control at a concentration of 200 ng/mL for 3 hours and then the cytoplasmic (top) and nuclear (bottom) protein was extracted and detected by Western blot assays. The images were analyzed by ImageJ (right). *: P

    Article Snippet: Cells cultured with Wnt2 recombinant protein (rWnt2, Abnova) or IgG control (Sino Biological Inc.) at a concentration of 200 ng/mL were seeded on coverslips for the indicated time points and then fixed in 4% paraformaldehyde at room temperature for 15 min.

    Techniques: Translocation Assay, Incubation, Concentration Assay, Western Blot

    ELISA competitive experiments showing that CIP inhibits the formation of the C3d–CR2/CD21 complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by anti-C3 pAb and HRP-conjugated secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: ELISA competitive experiments showing that CIP inhibits the formation of the C3d–CR2/CD21 complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by anti-C3 pAb and HRP-conjugated secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.

    Article Snippet: As shown in , binding of surface-coated C3d to both soluble CR2–CD21 and CIP was inhibited by increasing NaCl concentrations, suggesting an electrostatic contribution to CIP interaction with C3d.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Binding Assay

    Flow cytometry analysis of B-cell intracellular-calcium mobilization in presence or absence of CIP. A ) Diagram representation of the stimulus that triggers intracellular calcium increase (blue box); biotinylated anti-human IgM antibody (red box) is used to measure the substimulatory baseline before each experiment. The stimulus is designed to crosslink the B-cell receptor (BCR) to CR2/CD21. B , C ) Raji cells were preincubated with Fura Red, followed by the stimulus (see A ). Either 0.5–20 μM of CIP ( B ) or 20 μM of Fib3 ( C ) were preincubated with C3d–biotin–SA to assess their inhibition effects; changes in cellular fluorescence were monitored during 540 s and analyzed by flow cytometry. One representative of 3 experiments performed is shown.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: Flow cytometry analysis of B-cell intracellular-calcium mobilization in presence or absence of CIP. A ) Diagram representation of the stimulus that triggers intracellular calcium increase (blue box); biotinylated anti-human IgM antibody (red box) is used to measure the substimulatory baseline before each experiment. The stimulus is designed to crosslink the B-cell receptor (BCR) to CR2/CD21. B , C ) Raji cells were preincubated with Fura Red, followed by the stimulus (see A ). Either 0.5–20 μM of CIP ( B ) or 20 μM of Fib3 ( C ) were preincubated with C3d–biotin–SA to assess their inhibition effects; changes in cellular fluorescence were monitored during 540 s and analyzed by flow cytometry. One representative of 3 experiments performed is shown.

    Article Snippet: As shown in , binding of surface-coated C3d to both soluble CR2–CD21 and CIP was inhibited by increasing NaCl concentrations, suggesting an electrostatic contribution to CIP interaction with C3d.

    Techniques: Flow Cytometry, Inhibition, Fluorescence