recombinant sgpi 2 variants (New England Biolabs)
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Name:
SHuffle T7 Competent E coli
Description:
SHuffle T7 Competent E coli 12x0 05 ml
Catalog Number:
c3026j
Price:
228
Size:
12x0 05 ml
Category:
Competent Bacteria
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Structured Review
New England Biolabs
recombinant sgpi 2 variants
SHuffle T7 Competent E coli 12x0 05 ml
https://www.bioz.com/result/recombinant sgpi 2 variants/product/New England Biolabs
Average 91 stars, based on 48 article reviews
Price from $9.99 to $1999.99
SHuffle T7 Competent E coli 12x0 05 ml
https://www.bioz.com/result/recombinant sgpi 2 variants/product/New England Biolabs
Average 91 stars, based on 48 article reviews
Price from $9.99 to $1999.99
recombinant sgpi 2 variants - by Bioz Stars,
2020-07
91/100 stars
Related Products / Commonly Used Together
Images
1) Product Images from "Overlapping Specificity of Duplicated Human Pancreatic Elastase 3 Isoforms and Archetypal Porcine Elastase 1 Provides Clues to Evolution of Digestive Enzymes *"
Article Title: Overlapping Specificity of Duplicated Human Pancreatic Elastase 3 Isoforms and Archetypal Porcine Elastase 1 Provides Clues to Evolution of Digestive Enzymes *
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M116.770560
Figure Legend Snippet: Effect of the P1′ residue (Met versus Arg) on the inhibition of elastases and chymotrypsins by SGPI-2 variants E1 and E8 containing a P1 Leu. The graph shows pairwise comparisons of relative K D values of inhibitor binding to the indicated proteinases, normalized to the E1 value.
Techniques Used: Inhibition, Binding Assay
Figure Legend Snippet: Effect of the P2′ residue on the inhibition of elastases and chymotrypsins by SGPI-2 variants. A, inhibition by variants E2 and E7 containing a P1 Ile and a P2′ Leu versus Glu. Pairwise comparisons of relative K D values of inhibitor binding to the indicated proteinases are shown, normalized to the E2 value. ND , no inhibition was detected. B, inhibition by variants E1 and E9 containing a P1 Leu and a P2′ Leu versus Tyr. Pairwise comparisons of relative K D values normalized to the E1 value are shown. C, inhibition by variants E10 and E11 containing a P1 Leu, a P4 Ala, and a P2′ Leu versus Tyr. Pairwise comparisons of relative K D values normalized to the E10 value are shown.
Techniques Used: Inhibition, Binding Assay
Figure Legend Snippet: Effect of the P4′ residue (His versus Ala) on the inhibition of elastases and chymotrypsins by SGPI-2 variants E1 and E12 containing a P1 Leu. The graph shows pairwise comparisons of relative K D values of inhibitor binding to the indicated proteinases, normalized to the E1 value.
Techniques Used: Inhibition, Binding Assay
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Activity Assay:Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into Expressing:Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging Article Snippet: .. EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism * Article Snippet: .. 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EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in Plasmid Preparation:Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism * Article Snippet: .. Briefly, pro-VCC, which includes the 14-kDa prodomain (amino acid residues 26–741 of 82-kDa prepro-VCC) , was cloned into the pET32a(+) expression vector (Novagen) and transformed into Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into |