coli t7 express lysy  (New England Biolabs)


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    Name:
    T7 Express lysY Competent E coli High Efficiency
    Description:
    T7 Express lysY Competent E coli High Efficiency 6x0 2 ml
    Catalog Number:
    c3010i
    Price:
    173
    Category:
    Competent Bacteria
    Size:
    1 2 ml
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    Structured Review

    New England Biolabs coli t7 express lysy
    T7 Express lysY Competent E coli High Efficiency
    T7 Express lysY Competent E coli High Efficiency 6x0 2 ml
    https://www.bioz.com/result/coli t7 express lysy/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli t7 express lysy - by Bioz Stars, 2021-03
    94/100 stars

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    Clone Assay:

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Selection:

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Transformation Assay:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Article Title: Spy Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
    Article Snippet: These mutations were postulated to enable better binding and retention of SpyTag to SpyDock during the binding and washing steps, along with easier elution. .. Bacterial protein expression pET28a-SpyTag-MBP, pET28a-SpyTag002-MBP, and pET28a-SpyTag-mClover3 were transformed into chemically competent E. coli BL21 (DE3) RIPL (Agilent Technologies). pET28a-scPvuII-SpyTag was transformed into T7 Express lysY /I q (NEB). pDEST14-SpyCatcher2.1 S49C E77A (SpyDock), pDEST14-SpyCatcher2.1 S49C E77X variants, pDEST14-SpyCatcher2.1 E77A N-term Cys, pDEST14-SpyCatcher2.1 S55C E77A and pDEST14-SpyCatcher002-coiled coil fusions were transformed into chemically-competent E. coli C41 (DE3), a kind gift from Anthony Watts (University of Oxford). pET28a-αDR5-SpyTag was transformed into E. coli BL21 (DE3) RIPL containing a gene encoding phosphogluconolactonase, to degrade 6-phosphogluconolactone, which promotes protein gluconoylation . ..

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Plasmid Preparation:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Expressing:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Article Title: Spy Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
    Article Snippet: These mutations were postulated to enable better binding and retention of SpyTag to SpyDock during the binding and washing steps, along with easier elution. .. Bacterial protein expression pET28a-SpyTag-MBP, pET28a-SpyTag002-MBP, and pET28a-SpyTag-mClover3 were transformed into chemically competent E. coli BL21 (DE3) RIPL (Agilent Technologies). pET28a-scPvuII-SpyTag was transformed into T7 Express lysY /I q (NEB). pDEST14-SpyCatcher2.1 S49C E77A (SpyDock), pDEST14-SpyCatcher2.1 S49C E77X variants, pDEST14-SpyCatcher2.1 E77A N-term Cys, pDEST14-SpyCatcher2.1 S55C E77A and pDEST14-SpyCatcher002-coiled coil fusions were transformed into chemically-competent E. coli C41 (DE3), a kind gift from Anthony Watts (University of Oxford). pET28a-αDR5-SpyTag was transformed into E. coli BL21 (DE3) RIPL containing a gene encoding phosphogluconolactonase, to degrade 6-phosphogluconolactone, which promotes protein gluconoylation . ..

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Recombinant:

    Article Title: Biosimilars: the process is the product. The example of recombinant streptokinase
    Article Snippet: SUMOstar-streptokinase fusion sequences were sub-cloned into the EcoRV site of the expression vector pET-Blue-1 (Novagen (Merck), Darmstadt, Germany) by PCR. .. Recombinant streptokinase variants were expressed in T7 Express lysY E. coli (NEB, Herts, UK) and cells were lysed using B-PER Direct with enzymes (Thermo Fisher Scientific, Loughborough, UK). .. Recombinant SUMO star-streptokinase fusion proteins were purified by Ni-affinity and cleaved with SUMOstar Protease I (Lifesensors) over 4 h (assessed by SDS PAGE) at 30°C in the recommended buffer.

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Isolation:

    Article Title: Context-dependent function of a conserved translational regulatory module
    Article Snippet: All constructs were confirmed by direct sequencing. pIIIA/MS2-2- Ce-fem-3 , pIIIA/MS2-2- Ce-gld-1 , pIIIA/MS2-2-NRE and pACT2-FBF-2 (amino acids 121-632) are as previously described ( ). .. GST fusion proteins were isolated from T7 Express lysY competent E. coli (New England BioLabs) and purified using the following elution buffer: 1×PBS, 0.2% Tween 20, 150 mM NaCl, 0.1% 2-mercaptoethanol, 50 mM glutathione (reduced, pH 8.0). .. Twenty femtomoles 32 P end-labeled oligoribonucleotides (Dharmacon) were combined with GST-Cbr-PUF-2 or GST-Cbr-PUF-1.2 at various concentrations as described ( ).

    Purification:

    Article Title: Context-dependent function of a conserved translational regulatory module
    Article Snippet: All constructs were confirmed by direct sequencing. pIIIA/MS2-2- Ce-fem-3 , pIIIA/MS2-2- Ce-gld-1 , pIIIA/MS2-2-NRE and pACT2-FBF-2 (amino acids 121-632) are as previously described ( ). .. GST fusion proteins were isolated from T7 Express lysY competent E. coli (New England BioLabs) and purified using the following elution buffer: 1×PBS, 0.2% Tween 20, 150 mM NaCl, 0.1% 2-mercaptoethanol, 50 mM glutathione (reduced, pH 8.0). .. Twenty femtomoles 32 P end-labeled oligoribonucleotides (Dharmacon) were combined with GST-Cbr-PUF-2 or GST-Cbr-PUF-1.2 at various concentrations as described ( ).

    other:

    Article Title: Colorimetric Detection of Escherichia coli using Engineered Bacteriophage and an Affinity Reporter System
    Article Snippet: T7 Express competent E. coli (high efficiency) were purchased from New England BioLabs Magnetic macroporous bead cellulose (types MG) Iontosorb MG100 was purchased from Iontosorb.

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    • coli t7 express lysy  (New England Biolabs)
    94
    New England Biolabs coli t7 express lysy
    Coli T7 Express Lysy, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli t7 express lysy/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli t7 express lysy - by Bioz Stars, 2021-03
    94/100 stars
    		  Buy from Supplier

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