coli t7 express lysy  (New England Biolabs)


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    Name:
    T7 Express lysY Competent E coli High Efficiency
    Description:
    T7 Express lysY Competent E coli High Efficiency 6x0 2 ml
    Catalog Number:
    c3010i
    Price:
    173
    Category:
    Competent Bacteria
    Size:
    1 2 ml
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    Structured Review

    New England Biolabs coli t7 express lysy
    T7 Express lysY Competent E coli High Efficiency
    T7 Express lysY Competent E coli High Efficiency 6x0 2 ml
    https://www.bioz.com/result/coli t7 express lysy/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli t7 express lysy - by Bioz Stars, 2021-03
    94/100 stars

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    Clone Assay:

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Selection:

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Article Title: Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant
    Article Snippet: .. Wild-type or 42 amino acid deleted busR and downstream busAA promoter were cloned into pTCV-lac and introduced into E . coli T7 Express LysY (New England Biolabs) with selection using kanamycin (50 μg/ml). .. For β-galactosidase activity assays, strains were grown overnight in LB broth without NaCl (10 g/L tryptone; 5 g/L Yeast extract) and then diluted 1:100 in the same fresh LB medium and grown at 30°C, aeration 220 rpm to early log phase (OD600 ~0.25) where 0, 0.1, 0.2, 0.3 or 0.4 M NaCl was added.

    Transformation Assay:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Article Title: Spy Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
    Article Snippet: These mutations were postulated to enable better binding and retention of SpyTag to SpyDock during the binding and washing steps, along with easier elution. .. Bacterial protein expression pET28a-SpyTag-MBP, pET28a-SpyTag002-MBP, and pET28a-SpyTag-mClover3 were transformed into chemically competent E. coli BL21 (DE3) RIPL (Agilent Technologies). pET28a-scPvuII-SpyTag was transformed into T7 Express lysY /I q (NEB). pDEST14-SpyCatcher2.1 S49C E77A (SpyDock), pDEST14-SpyCatcher2.1 S49C E77X variants, pDEST14-SpyCatcher2.1 E77A N-term Cys, pDEST14-SpyCatcher2.1 S55C E77A and pDEST14-SpyCatcher002-coiled coil fusions were transformed into chemically-competent E. coli C41 (DE3), a kind gift from Anthony Watts (University of Oxford). pET28a-αDR5-SpyTag was transformed into E. coli BL21 (DE3) RIPL containing a gene encoding phosphogluconolactonase, to degrade 6-phosphogluconolactone, which promotes protein gluconoylation . ..

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Plasmid Preparation:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Expressing:

    Article Title: Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum
    Article Snippet: The plasmids and primers used for cloning are listed in Tables S1 and S2 in the supplemental material. .. E. coli competent cells (T7 Express lysY/I q ; NEB) were transformed as previously described , using the pEXP5-NT/TOPO (Invitrogen) plasmid backbone for AdhA protein expression. ..

    Article Title: Spy Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
    Article Snippet: These mutations were postulated to enable better binding and retention of SpyTag to SpyDock during the binding and washing steps, along with easier elution. .. Bacterial protein expression pET28a-SpyTag-MBP, pET28a-SpyTag002-MBP, and pET28a-SpyTag-mClover3 were transformed into chemically competent E. coli BL21 (DE3) RIPL (Agilent Technologies). pET28a-scPvuII-SpyTag was transformed into T7 Express lysY /I q (NEB). pDEST14-SpyCatcher2.1 S49C E77A (SpyDock), pDEST14-SpyCatcher2.1 S49C E77X variants, pDEST14-SpyCatcher2.1 E77A N-term Cys, pDEST14-SpyCatcher2.1 S55C E77A and pDEST14-SpyCatcher002-coiled coil fusions were transformed into chemically-competent E. coli C41 (DE3), a kind gift from Anthony Watts (University of Oxford). pET28a-αDR5-SpyTag was transformed into E. coli BL21 (DE3) RIPL containing a gene encoding phosphogluconolactonase, to degrade 6-phosphogluconolactone, which promotes protein gluconoylation . ..

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Recombinant:

    Article Title: Biosimilars: the process is the product. The example of recombinant streptokinase
    Article Snippet: SUMOstar-streptokinase fusion sequences were sub-cloned into the EcoRV site of the expression vector pET-Blue-1 (Novagen (Merck), Darmstadt, Germany) by PCR. .. Recombinant streptokinase variants were expressed in T7 Express lysY E. coli (NEB, Herts, UK) and cells were lysed using B-PER Direct with enzymes (Thermo Fisher Scientific, Loughborough, UK). .. Recombinant SUMO star-streptokinase fusion proteins were purified by Ni-affinity and cleaved with SUMOstar Protease I (Lifesensors) over 4 h (assessed by SDS PAGE) at 30°C in the recommended buffer.

    Article Title: Methionine Sulfoxide Reductases Are Essential for Virulence of Salmonella Typhimurium
    Article Snippet: Primers ( ) included restriction sites for subsequent cloning into the T7 overexpression vector pET28c (Novagen, ) according to manufacturer's instructions. .. Plasmids, designated pET28c::msrA and pET28c::msrB , respectively, were transformed into E. coli T7 lysY Express (NEB) for expression of the recombinant His-tagged proteins. .. Expression of MsrA and MsrB was induced by addition of 0.5 mM IPTG at an OD600 ∼0,8, and cells were grown for another 5 h. Bacteria were harvested by centrifugation (3000×g, 25 min, 4°C) and resuspended in binding buffer containing 50 mM NaH2 PO4 , 300 mM NaCl and 10 mM imidazole (pH 8.0 for MsrA, pH 8.5 for MsrB).

    Isolation:

    Article Title: Context-dependent function of a conserved translational regulatory module
    Article Snippet: All constructs were confirmed by direct sequencing. pIIIA/MS2-2- Ce-fem-3 , pIIIA/MS2-2- Ce-gld-1 , pIIIA/MS2-2-NRE and pACT2-FBF-2 (amino acids 121-632) are as previously described ( ). .. GST fusion proteins were isolated from T7 Express lysY competent E. coli (New England BioLabs) and purified using the following elution buffer: 1×PBS, 0.2% Tween 20, 150 mM NaCl, 0.1% 2-mercaptoethanol, 50 mM glutathione (reduced, pH 8.0). .. Twenty femtomoles 32 P end-labeled oligoribonucleotides (Dharmacon) were combined with GST-Cbr-PUF-2 or GST-Cbr-PUF-1.2 at various concentrations as described ( ).

    Purification:

    Article Title: Context-dependent function of a conserved translational regulatory module
    Article Snippet: All constructs were confirmed by direct sequencing. pIIIA/MS2-2- Ce-fem-3 , pIIIA/MS2-2- Ce-gld-1 , pIIIA/MS2-2-NRE and pACT2-FBF-2 (amino acids 121-632) are as previously described ( ). .. GST fusion proteins were isolated from T7 Express lysY competent E. coli (New England BioLabs) and purified using the following elution buffer: 1×PBS, 0.2% Tween 20, 150 mM NaCl, 0.1% 2-mercaptoethanol, 50 mM glutathione (reduced, pH 8.0). .. Twenty femtomoles 32 P end-labeled oligoribonucleotides (Dharmacon) were combined with GST-Cbr-PUF-2 or GST-Cbr-PUF-1.2 at various concentrations as described ( ).

    other:

    Article Title: Colorimetric Detection of Escherichia coli using Engineered Bacteriophage and an Affinity Reporter System
    Article Snippet: T7 Express competent E. coli (high efficiency) were purchased from New England BioLabs Magnetic macroporous bead cellulose (types MG) Iontosorb MG100 was purchased from Iontosorb.

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  • 94
    New England Biolabs coli t7 express lysy
    Coli T7 Express Lysy, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli t7 express lysy/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli t7 express lysy - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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