melittin gene  (New England Biolabs)


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    Name:
    NEB 5 alpha F Iq Competent E coli High Efficiency
    Description:
    NEB 5 alpha F Iq Competent E coli High Efficiency 20x0 05 ml
    Catalog Number:
    c2992h
    Price:
    198
    Size:
    1 ml
    Category:
    Competent Bacteria
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    Structured Review

    New England Biolabs melittin gene
    NEB 5 alpha F Iq Competent E coli High Efficiency
    NEB 5 alpha F Iq Competent E coli High Efficiency 20x0 05 ml
    https://www.bioz.com/result/melittin gene/product/New England Biolabs
    Average 90 stars, based on 18053 article reviews
    Price from $9.99 to $1999.99
    melittin gene - by Bioz Stars, 2020-07
    90/100 stars

    Images

    1) Product Images from "Genetically Engineered Yeast Expressing a Lytic Peptide from Bee Venom (Melittin) Kills Symbiotic Protozoa in the Gut of Formosan Subterranean Termites"

    Article Title: Genetically Engineered Yeast Expressing a Lytic Peptide from Bee Venom (Melittin) Kills Symbiotic Protozoa in the Gut of Formosan Subterranean Termites

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0151675

    Test for gut integrity using workers fed with blue food dye on bait containing cellulose only (Control) or yeast expressing Melittin (Treatment).
    Figure Legend Snippet: Test for gut integrity using workers fed with blue food dye on bait containing cellulose only (Control) or yeast expressing Melittin (Treatment).

    Techniques Used: Expressing

    Changes in health of gut and protozoa of Formosan subterranean termite workers after feeding bait containing live yeast expressing Melittin, fluorescent protein (mPlum) or plain cellulose without yeast. Error bars represent 95% confidence intervals; non-overlapping confidence intervals signify significant difference among treatments.
    Figure Legend Snippet: Changes in health of gut and protozoa of Formosan subterranean termite workers after feeding bait containing live yeast expressing Melittin, fluorescent protein (mPlum) or plain cellulose without yeast. Error bars represent 95% confidence intervals; non-overlapping confidence intervals signify significant difference among treatments.

    Techniques Used: Expressing

    Related Articles

    Clone Assay:

    Article Title: Protozoacidal Trojan-Horse: Use of a Ligand-Lytic Peptide for Selective Destruction of Symbiotic Protozoa within Termite Guts
    Article Snippet: .. All three constructs were cloned into competent E. coli cells (NEB # C2992, New England Biolabs Inc., MA, USA). .. Each vector was isolated and digested with a pair of respective restriction endonucleases to determine the presence of the insert.

    Ligation:

    Article Title: Endogenous viral element-derived piRNAs are not required for production of ping-pong-dependent piRNAs from Diaphorina citri densovirus
    Article Snippet: .. The ligation product was transformed into chemically competent E. coli DH5α and plasmids were purified as described. .. Transfection and infection of S2 Cells and virion purification pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts).

    Construct:

    Article Title: Protozoacidal Trojan-Horse: Use of a Ligand-Lytic Peptide for Selective Destruction of Symbiotic Protozoa within Termite Guts
    Article Snippet: .. All three constructs were cloned into competent E. coli cells (NEB # C2992, New England Biolabs Inc., MA, USA). .. Each vector was isolated and digested with a pair of respective restriction endonucleases to determine the presence of the insert.

    Purification:

    Article Title: Endogenous viral element-derived piRNAs are not required for production of ping-pong-dependent piRNAs from Diaphorina citri densovirus
    Article Snippet: .. The ligation product was transformed into chemically competent E. coli DH5α and plasmids were purified as described. .. Transfection and infection of S2 Cells and virion purification pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts).

    Incubation:

    Article Title: CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon
    Article Snippet: .. T4 ligase was added, and the reaction containing digested gDNA and ligase was incubated overnight at 16°C, then transformed into 5-alpha F’I q High Efficiency Competent E . coli cells (NEB, C2992H), and transformants were selected on media supplemented with TMP. .. Lastly, “rescued” plasmids from multiple transformants were isolated and subjected to Sanger sequencing.

    Alkaline Lysis:

    Article Title: In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy
    Article Snippet: .. Plasmids were transformed in E. coli bacteria NEB 5-alpha (New England Biolabs, C2992) or Xl10-GOLD (Agilent Technologies, 200314) and extracted by alkaline lysis with Nucleobond PC100 Midiprep kits (Macherey-Nagel, 740573). .. For generation of GST fusion proteins, full-length mouse Vdac1 (NM_011694.4), Tomm7 , Slc25a31 , Cox5b , Mdh2 , Pink1 ( ) and Pink1 epitopes were amplified by PCR and cloned in pGEX 4T1 (GE Healthcare Life Sciences, 28-9545-49).

    Sequencing:

    Article Title: The RNA of maize chlorotic mottle virus - the essential virus in maize lethal necrosis disease - is translated via a panicum mosaic virus-like cap-independent translation element
    Article Snippet: .. DNA was added to competent E. coli DH5α, selected on ampicillin/XGal plates, and screened by restriction digests and sequencing. ..

    Transformation Assay:

    Article Title: Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries
    Article Snippet: .. The recombination reaction mix was transformed into NEB 5-alpha F'Iq cells (NEB) for the LPLlac -trap library and NEB 10 beta cells (NEB) for the LPL-trap library. ..

    Article Title: CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon
    Article Snippet: .. T4 ligase was added, and the reaction containing digested gDNA and ligase was incubated overnight at 16°C, then transformed into 5-alpha F’I q High Efficiency Competent E . coli cells (NEB, C2992H), and transformants were selected on media supplemented with TMP. .. Lastly, “rescued” plasmids from multiple transformants were isolated and subjected to Sanger sequencing.

    Article Title: Endogenous viral element-derived piRNAs are not required for production of ping-pong-dependent piRNAs from Diaphorina citri densovirus
    Article Snippet: .. The ligation product was transformed into chemically competent E. coli DH5α and plasmids were purified as described. .. Transfection and infection of S2 Cells and virion purification pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts).

    Article Title: A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution
    Article Snippet: .. The assemblies were transformed into NEB® 5-alpha F' I q competent Escherichia coli (New England Biolabs) according to the manufacturer's instructions. .. Clones were screened by diagnostic restriction digest and those that were correctly assembled were confirmed using Sanger sequencing performed by GENEWIZ, Inc. Deletion of pobAR from the P. putida KT2440 genome using pCJ041 was confirmed by amplification of a 2094 bp product in diagnostic colony PCR using MyTaq™ HS Red Mix (Bioline) with primers oCJ298 (5′-ACCTTTCATCTGCGGACC-3′) and oCJ299 (5′-ATCTGTGGCACCCACTTG-3′) rather than the 3942 bp product generated when pobA and pobR are present.

    Article Title: In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy
    Article Snippet: .. Plasmids were transformed in E. coli bacteria NEB 5-alpha (New England Biolabs, C2992) or Xl10-GOLD (Agilent Technologies, 200314) and extracted by alkaline lysis with Nucleobond PC100 Midiprep kits (Macherey-Nagel, 740573). .. For generation of GST fusion proteins, full-length mouse Vdac1 (NM_011694.4), Tomm7 , Slc25a31 , Cox5b , Mdh2 , Pink1 ( ) and Pink1 epitopes were amplified by PCR and cloned in pGEX 4T1 (GE Healthcare Life Sciences, 28-9545-49).

    Plasmid Preparation:

    Article Title: Determinants of RNA recognition by the FinO domain of the Escherichia coli ProQ protein
    Article Snippet: .. NEB 5-alpha F’Iq cells (New England Biolabs) were used as the recipient strain for all plasmid constructions. ..

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    New England Biolabs e coli bacteria neb 5 alpha
    E Coli Bacteria Neb 5 Alpha, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bacteria neb 5 alpha/product/New England Biolabs
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    e coli bacteria neb 5 alpha - by Bioz Stars, 2020-07
    99/100 stars
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