amplicon  (New England Biolabs)


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  • 94
    Name:
    NEB 5 alpha Electrocompetent E coli
    Description:
    NEB 5 alpha Electrocompetent E coli 6x0 1 ml
    Catalog Number:
    c2989k
    Price:
    185
    Size:
    0 6 ml
    Category:
    Competent Bacteria
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    Structured Review

    New England Biolabs amplicon
    NEB 5 alpha Electrocompetent E coli
    NEB 5 alpha Electrocompetent E coli 6x0 1 ml
    https://www.bioz.com/result/amplicon/product/New England Biolabs
    Average 94 stars, based on 5075 article reviews
    Price from $9.99 to $1999.99
    amplicon - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Electroporation:

    Article Title: Low-N protein engineering with data-efficient deep learning
    Article Snippet: .. All library clonings and transformations were performed using the following general steps: 1) PCR of the vector backbone, 2) golden gate assembly of the insert and vector, 3) ethanol precipitation of the ligated plasmid, 4) electroporation into electrocompetent DH5α E. coli , recovery, and subsequent outgrowth under selection. .. Vector PCRs were performed with primers adjacent to the insert region that extended into the vector backbone.

    Article Title: Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast
    Article Snippet: .. Plasmid DNA was isolated from positive transformants using a Zymoprep™ Yeast Plasmid Miniprep II Kit (Zymo Research) and used to transform NEB 5-alpha Electrocompetent E. coli (New England Biolabs) by electroporation. .. Plasmid DNA was isolated from the positive clones grown on LB-ampicillin using the alkaline lysis method and further verified by DNA sequencing.

    Article Title: Antibiotic pressure on the acquisition and loss of antibiotic resistance genes in Klebsiella pneumoniae
    Article Snippet: .. Briefly, transformants were obtained by electroporation (Gene Pulser, 1.7 kV, 25 μF, 200 ohms) of 3 μL of total plasmid DNA with 50 μL of electrocompetent NEB 5-alpha Escherichia coli recipient and selected using agar plates containing 100 mg/L ampicillin. ..

    Selection:

    Article Title: Low-N protein engineering with data-efficient deep learning
    Article Snippet: .. All library clonings and transformations were performed using the following general steps: 1) PCR of the vector backbone, 2) golden gate assembly of the insert and vector, 3) ethanol precipitation of the ligated plasmid, 4) electroporation into electrocompetent DH5α E. coli , recovery, and subsequent outgrowth under selection. .. Vector PCRs were performed with primers adjacent to the insert region that extended into the vector backbone.

    Clone Assay:

    Article Title: Engineering orthogonal dual transcription factors for multi-input synthetic promoters
    Article Snippet: .. Combinatorial library cloning was performed with NEB 5-alpha electrocompetent cells or electrocompetent TG1 cells. .. Selection phage production was carried out with BL21(DE3) cells (NEB).

    Article Title: Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity
    Article Snippet: .. These mutagenized amplicons were cloned into the sRNA expression vector by restriction and ligation, and transformed at high efficiency into 5-alpha electrocompetent cells (NEB). ..

    Ligation:

    Article Title: Systematic Study of Nucleosome-Displacing Factors in Budding Yeast
    Article Snippet: .. The ligation product was concentrated through a spin column into a 30μl volume and transformed into 5-alpha Electrocompetent E. coli (NEB). .. A total volume of 250μl electrocompetent cells transformed with 25μl ligation product typically produced ~2,000,000 colonies.

    Article Title: Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity
    Article Snippet: .. These mutagenized amplicons were cloned into the sRNA expression vector by restriction and ligation, and transformed at high efficiency into 5-alpha electrocompetent cells (NEB). ..

    Ethanol Precipitation:

    Article Title: Low-N protein engineering with data-efficient deep learning
    Article Snippet: .. All library clonings and transformations were performed using the following general steps: 1) PCR of the vector backbone, 2) golden gate assembly of the insert and vector, 3) ethanol precipitation of the ligated plasmid, 4) electroporation into electrocompetent DH5α E. coli , recovery, and subsequent outgrowth under selection. .. Vector PCRs were performed with primers adjacent to the insert region that extended into the vector backbone.

    Purification:

    Article Title: Mutant APH(2?)-IIa Enzymes with Increased Activity against Amikacin and Isepamicin ▿
    Article Snippet: .. Purified plasmid DNA of the pHF022 vector containing the mutagenized gene for APH(2″)-IIa was used to transform electrocompetent E. coli NEB 5-alpha cells (NEB catalog no. C2989K). .. After overnight incubation in LB broth supplemented with 100 μg/ml of ampicillin (selection marker for the pHF022 vector), the bacterial culture was diluted 500-fold into fresh LB broth supplemented with amikacin or isepamicin at various concentrations.

    other:

    Article Title: A tiling1deletion based genetic screen for cis-regulatory element identification in mammalian cells
    Article Snippet: The ligates was electro-transformed into 5-alpha Electrocompetent E. coli and plated on Agar plates.

    Expressing:

    Article Title: Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity
    Article Snippet: .. These mutagenized amplicons were cloned into the sRNA expression vector by restriction and ligation, and transformed at high efficiency into 5-alpha electrocompetent cells (NEB). ..

    Isolation:

    Article Title: Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast
    Article Snippet: .. Plasmid DNA was isolated from positive transformants using a Zymoprep™ Yeast Plasmid Miniprep II Kit (Zymo Research) and used to transform NEB 5-alpha Electrocompetent E. coli (New England Biolabs) by electroporation. .. Plasmid DNA was isolated from the positive clones grown on LB-ampicillin using the alkaline lysis method and further verified by DNA sequencing.

    Polymerase Chain Reaction:

    Article Title: Low-N protein engineering with data-efficient deep learning
    Article Snippet: .. All library clonings and transformations were performed using the following general steps: 1) PCR of the vector backbone, 2) golden gate assembly of the insert and vector, 3) ethanol precipitation of the ligated plasmid, 4) electroporation into electrocompetent DH5α E. coli , recovery, and subsequent outgrowth under selection. .. Vector PCRs were performed with primers adjacent to the insert region that extended into the vector backbone.

    Transformation Assay:

    Article Title: Systematic Study of Nucleosome-Displacing Factors in Budding Yeast
    Article Snippet: .. The ligation product was concentrated through a spin column into a 30μl volume and transformed into 5-alpha Electrocompetent E. coli (NEB). .. A total volume of 250μl electrocompetent cells transformed with 25μl ligation product typically produced ~2,000,000 colonies.

    Article Title: Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity
    Article Snippet: .. These mutagenized amplicons were cloned into the sRNA expression vector by restriction and ligation, and transformed at high efficiency into 5-alpha electrocompetent cells (NEB). ..

    Plasmid Preparation:

    Article Title: Low-N protein engineering with data-efficient deep learning
    Article Snippet: .. All library clonings and transformations were performed using the following general steps: 1) PCR of the vector backbone, 2) golden gate assembly of the insert and vector, 3) ethanol precipitation of the ligated plasmid, 4) electroporation into electrocompetent DH5α E. coli , recovery, and subsequent outgrowth under selection. .. Vector PCRs were performed with primers adjacent to the insert region that extended into the vector backbone.

    Article Title: Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity
    Article Snippet: .. These mutagenized amplicons were cloned into the sRNA expression vector by restriction and ligation, and transformed at high efficiency into 5-alpha electrocompetent cells (NEB). ..

    Article Title: Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast
    Article Snippet: .. Plasmid DNA was isolated from positive transformants using a Zymoprep™ Yeast Plasmid Miniprep II Kit (Zymo Research) and used to transform NEB 5-alpha Electrocompetent E. coli (New England Biolabs) by electroporation. .. Plasmid DNA was isolated from the positive clones grown on LB-ampicillin using the alkaline lysis method and further verified by DNA sequencing.

    Article Title: Mutant APH(2?)-IIa Enzymes with Increased Activity against Amikacin and Isepamicin ▿
    Article Snippet: .. Purified plasmid DNA of the pHF022 vector containing the mutagenized gene for APH(2″)-IIa was used to transform electrocompetent E. coli NEB 5-alpha cells (NEB catalog no. C2989K). .. After overnight incubation in LB broth supplemented with 100 μg/ml of ampicillin (selection marker for the pHF022 vector), the bacterial culture was diluted 500-fold into fresh LB broth supplemented with amikacin or isepamicin at various concentrations.

    Article Title: Antibiotic pressure on the acquisition and loss of antibiotic resistance genes in Klebsiella pneumoniae
    Article Snippet: .. Briefly, transformants were obtained by electroporation (Gene Pulser, 1.7 kV, 25 μF, 200 ohms) of 3 μL of total plasmid DNA with 50 μL of electrocompetent NEB 5-alpha Escherichia coli recipient and selected using agar plates containing 100 mg/L ampicillin. ..

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  • 99
    New England Biolabs neb 5 alpha electrocompetent e coli
    Neb 5 Alpha Electrocompetent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb 5 alpha electrocompetent e coli/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    neb 5 alpha electrocompetent e coli - by Bioz Stars, 2020-08
    99/100 stars
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