bl21 (New England Biolabs)


Structured Review

Bl21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bl21/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Redox environment is an intracellular factor to operate distinct pathways for aggregation of Cu,Zn-superoxide dismutase in amyotrophic lateral sclerosis"
Article Title: Redox environment is an intracellular factor to operate distinct pathways for aggregation of Cu,Zn-superoxide dismutase in amyotrophic lateral sclerosis
Journal: Frontiers in Cellular Neuroscience
doi: 10.3389/fncel.2013.00240

Figure Legend Snippet: Aggregation of disulfide-reduced SOD1 with fALS mutations in E. coli BL21 was not rescued by addition of ZnSO 4 . E. coli BL21(DE3) was transformed with pET15b harboring human SOD1 cDNA with indicated fALS mutations, and the protein expression was induced by IPTG in the presence of 1 mM ZnSO 4 . Cell lysates were fractionated into soluble supernatant (s) and insoluble pellets (p), treated with iodoacetamide, and then analyzed with non-reducing SDS-PAGE by using a 15% polyacrylamide gel. White (SOD1 S-S ) and black (SOD1 SH ) arrows at the right side of the gel image indicate positions of bands corresponding to SOD1 with and without a disulfide bond, respectively.
Techniques Used: Transformation Assay, Expressing, SDS Page

Figure Legend Snippet: Amyloid-like characters of SOD1 aggregates purified from insoluble inclusions in E. coli . (A) SOD1(G37R) aggregates purified from insoluble inclusions in E. coli were reacted with iodoacetamide for protection of free thiol groups and analyzed with reducing (+β-ME) and non-reducing (+IA) SDS-PAGE. Disulfide-crosslinked oligomers were identified in SOD1(G37R) aggregates purified from insoluble inclusions in E. coli SHuffle TM but not in BL21(DE3). (B, C) Tinctorial properties of SOD1(G37R) aggregates purified from E. coli BL21 and SHuffle TM (red and blue curves, respectively) were examined by (B) fluorescence of thioflavin T and (C) absorption of Congo red. Black curves represent (B) fluorescence spectrum of thioflavin T and (C) absorption spectrum of Congo red without addition of SOD1 aggregates. (D, E) Electron micrograms of SOD1(G37R) aggregates purified from E. coli (D) BL21 and (E) SHuffle TM . SOD1(G37R) aggregates exhibit large, amorphous morphologies (left panels), while fibrillar structures become evident after brief treatment of aggregates with Proteinase K (right panels). A bar represents 2 μm (left panels) or 50 nm (right panels).
Techniques Used: Purification, IA, SDS Page, Fluorescence
2) Product Images from "Safe Recombinant Outer Membrane Vesicles that Display M2e Elicit Heterologous Influenza Protection"
Article Title: Safe Recombinant Outer Membrane Vesicles that Display M2e Elicit Heterologous Influenza Protection
Journal: Molecular Therapy
doi: 10.1016/j.ymthe.2017.01.010

Figure Legend Snippet: Pyrogenicity and TLR Activity Are rOMV Source Strain Dependent (A) Pyrogenicity (measured in endotoxin units) of CC, Nsl, and BL21 rOMVS determined using whole-blood pyrogenicity test. Groups were compared with Kruskal-Wallis test, followed by Mann-Whitney between pairs, using Bonferroni method to account for multiple comparisons (*p
Techniques Used: Activity Assay, MANN-WHITNEY

Figure Legend Snippet: rOMVs Produced from Three Different E. coli Strains Are Structurally Comparable (A–C) TEM images of rOMVs stained with uranyl acetate: CC rOMVs (A), BL21 rOMVs (B), and Nsl rOMVs (C). Scale bars represent 100 nm. (D and E) Total IgG (D) and isotypes IgG1 and IgG2a (E) anti-GFP titers from BALB/c mice 8 weeks post prime dose of ClyA-GFP-expressing CC or BL21 rOMVs. Titer error bars represent 95% confidence intervals (CI) of geometric mean. Log-transformed data analyzed using an unpaired Student’s t test to compare CC versus BL21 rOMV anti-GFP IgG levels and using paired Student’s t test to compare IgG1:IgG2a levels for each rOMV type. Dotted line indicates titer of sera from mice pre-vaccination.
Techniques Used: Produced, Transmission Electron Microscopy, Staining, Mouse Assay, Expressing, Transformation Assay