nico21  (New England Biolabs)


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  • 99
    Name:
    NiCo21 DE3 Competent E coli
    Description:
    NiCo21 DE3 Competent E coli 20x0 05 ml
    Catalog Number:
    C2529H
    Price:
    380
    Category:
    Competent Bacteria
    Size:
    1 ml
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    Structured Review

    New England Biolabs nico21
    NiCo21 DE3 Competent E coli
    NiCo21 DE3 Competent E coli 20x0 05 ml
    https://www.bioz.com/result/nico21/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nico21 - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Crystal structure of a dimerization domain of human Caprin-2: similar overall dimeric fold but different molecular surface properties to that of human Caprin-1
    Article Snippet: .. The recombinant plasmid was transformed into NiCo21(DE3) E. coli cells (New England Biolabs). .. Cell culture was grown in LB media until it reached an OD600 of 0.6–0.8.

    Article Title: Identification of an Important Orphan Histidine Kinase for the Initiation of Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain SM101
    Article Snippet: For sporulating cultures of C. perfringens , all strains were grown in modified Duncan-Strong medium (MDS) supplemented with caffeine and raffinose (1.5% proteose peptone [Becton, Dickinson], 0.4% yeast extract [Becton, Dickinson], 1% disodium phosphate heptahydrate [Fisher Scientific], 0.1% sodium thioglycolate [Sigma-Aldrich], 1 mM caffeine [Sigma-Aldrich], 0.4% raffinose [Sigma-Aldrich]). .. E. coli strains DH5α and NiCo21(DE3) (New England Biolabs) were used as a cloning host and for recombinant protein expression and purification. .. E. coli was grown in Luria-Bertani broth (LB; 1% tryptone [Becton, Dickinson], 0.5% yeast extract [Becton, Dickinson], 1% NaCl [Fisher Scientific]) or on LB agar (LB containing 1.5% agar).

    Article Title: The chloroplast 2-cysteine peroxiredoxin functions as thioredoxin oxidase in redox regulation of chloroplast metabolism
    Article Snippet: .. Production and purification of recombinant proteins The recombinant plasmids were introduced into the E. coli NiCo21 (DE3) strain (NEB). ..

    Article Title: Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
    Article Snippet: Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells.

    Plasmid Preparation:

    Article Title: Crystal structure of a dimerization domain of human Caprin-2: similar overall dimeric fold but different molecular surface properties to that of human Caprin-1
    Article Snippet: .. The recombinant plasmid was transformed into NiCo21(DE3) E. coli cells (New England Biolabs). .. Cell culture was grown in LB media until it reached an OD600 of 0.6–0.8.

    Article Title: Thiol Redox Regulation of Plant β-Carbonic Anhydrase.
    Article Snippet: .. Heterologous Expression and PurificationE. coli NiCo21 (DE3) cells were transformed with plasmid DNA. .. After induction with 100 µM isopropyl-β-D-thiogalactopyranoside, recombinant protein expression was carried out for 4 h at 37 ◦C and 170 rpm.

    Article Title: External Control Viral-Like Particle Construction for Detection of Emergent Arboviruses by Real-Time Reverse-Transcription PCR
    Article Snippet: The derived vector (a unique plasmid with all targets) is referred to as pET47b(+)-MS2-ZDC. .. External Control Production The pET47b(+)-MS2-ZDC vector was transformed into NiCo21(DE3)-competent Escherichia coli in accordance with the manufacturer's instructions (New England Biolabs, USA). ..

    Transformation Assay:

    Article Title: Crystal structure of a dimerization domain of human Caprin-2: similar overall dimeric fold but different molecular surface properties to that of human Caprin-1
    Article Snippet: .. The recombinant plasmid was transformed into NiCo21(DE3) E. coli cells (New England Biolabs). .. Cell culture was grown in LB media until it reached an OD600 of 0.6–0.8.

    Article Title: Thiol Redox Regulation of Plant β-Carbonic Anhydrase.
    Article Snippet: .. Heterologous Expression and PurificationE. coli NiCo21 (DE3) cells were transformed with plasmid DNA. .. After induction with 100 µM isopropyl-β-D-thiogalactopyranoside, recombinant protein expression was carried out for 4 h at 37 ◦C and 170 rpm.

    Article Title: External Control Viral-Like Particle Construction for Detection of Emergent Arboviruses by Real-Time Reverse-Transcription PCR
    Article Snippet: The derived vector (a unique plasmid with all targets) is referred to as pET47b(+)-MS2-ZDC. .. External Control Production The pET47b(+)-MS2-ZDC vector was transformed into NiCo21(DE3)-competent Escherichia coli in accordance with the manufacturer's instructions (New England Biolabs, USA). ..

    Clone Assay:

    Article Title: Identification of an Important Orphan Histidine Kinase for the Initiation of Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain SM101
    Article Snippet: For sporulating cultures of C. perfringens , all strains were grown in modified Duncan-Strong medium (MDS) supplemented with caffeine and raffinose (1.5% proteose peptone [Becton, Dickinson], 0.4% yeast extract [Becton, Dickinson], 1% disodium phosphate heptahydrate [Fisher Scientific], 0.1% sodium thioglycolate [Sigma-Aldrich], 1 mM caffeine [Sigma-Aldrich], 0.4% raffinose [Sigma-Aldrich]). .. E. coli strains DH5α and NiCo21(DE3) (New England Biolabs) were used as a cloning host and for recombinant protein expression and purification. .. E. coli was grown in Luria-Bertani broth (LB; 1% tryptone [Becton, Dickinson], 0.5% yeast extract [Becton, Dickinson], 1% NaCl [Fisher Scientific]) or on LB agar (LB containing 1.5% agar).

    Expressing:

    Article Title: Identification of an Important Orphan Histidine Kinase for the Initiation of Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain SM101
    Article Snippet: For sporulating cultures of C. perfringens , all strains were grown in modified Duncan-Strong medium (MDS) supplemented with caffeine and raffinose (1.5% proteose peptone [Becton, Dickinson], 0.4% yeast extract [Becton, Dickinson], 1% disodium phosphate heptahydrate [Fisher Scientific], 0.1% sodium thioglycolate [Sigma-Aldrich], 1 mM caffeine [Sigma-Aldrich], 0.4% raffinose [Sigma-Aldrich]). .. E. coli strains DH5α and NiCo21(DE3) (New England Biolabs) were used as a cloning host and for recombinant protein expression and purification. .. E. coli was grown in Luria-Bertani broth (LB; 1% tryptone [Becton, Dickinson], 0.5% yeast extract [Becton, Dickinson], 1% NaCl [Fisher Scientific]) or on LB agar (LB containing 1.5% agar).

    Article Title: Thiol Redox Regulation of Plant β-Carbonic Anhydrase.
    Article Snippet: .. Heterologous Expression and PurificationE. coli NiCo21 (DE3) cells were transformed with plasmid DNA. .. After induction with 100 µM isopropyl-β-D-thiogalactopyranoside, recombinant protein expression was carried out for 4 h at 37 ◦C and 170 rpm.

    Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Article Snippet: The SUMO-His6 tagged C9orf72 expressed as inclusion bodies and the construct with the minimal tag (His6 ) did not express at all in E. coli . .. A selection of bacterial expression strains was tried (such as Shuffle T7 express and Nico21(DE3) (from New England Biolabs, Hitchin, UK), Rosetta strains, Rosettagami strains (from Novagen, Watford, UK), SoluBL21 (from Amsbio, Abingdon, UK)), along with a variety of media types and expression trials varying different parameters, but none managed to improve expression levels or produced protein that was stable. .. We also prepared constructs where maltose-binding protein (MBP) and GST tags were moved to the C-terminal end of the protein but obtained similar results as above.

    Purification:

    Article Title: Identification of an Important Orphan Histidine Kinase for the Initiation of Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain SM101
    Article Snippet: For sporulating cultures of C. perfringens , all strains were grown in modified Duncan-Strong medium (MDS) supplemented with caffeine and raffinose (1.5% proteose peptone [Becton, Dickinson], 0.4% yeast extract [Becton, Dickinson], 1% disodium phosphate heptahydrate [Fisher Scientific], 0.1% sodium thioglycolate [Sigma-Aldrich], 1 mM caffeine [Sigma-Aldrich], 0.4% raffinose [Sigma-Aldrich]). .. E. coli strains DH5α and NiCo21(DE3) (New England Biolabs) were used as a cloning host and for recombinant protein expression and purification. .. E. coli was grown in Luria-Bertani broth (LB; 1% tryptone [Becton, Dickinson], 0.5% yeast extract [Becton, Dickinson], 1% NaCl [Fisher Scientific]) or on LB agar (LB containing 1.5% agar).

    Article Title: The chloroplast 2-cysteine peroxiredoxin functions as thioredoxin oxidase in redox regulation of chloroplast metabolism
    Article Snippet: .. Production and purification of recombinant proteins The recombinant plasmids were introduced into the E. coli NiCo21 (DE3) strain (NEB). ..

    Selection:

    Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Article Snippet: The SUMO-His6 tagged C9orf72 expressed as inclusion bodies and the construct with the minimal tag (His6 ) did not express at all in E. coli . .. A selection of bacterial expression strains was tried (such as Shuffle T7 express and Nico21(DE3) (from New England Biolabs, Hitchin, UK), Rosetta strains, Rosettagami strains (from Novagen, Watford, UK), SoluBL21 (from Amsbio, Abingdon, UK)), along with a variety of media types and expression trials varying different parameters, but none managed to improve expression levels or produced protein that was stable. .. We also prepared constructs where maltose-binding protein (MBP) and GST tags were moved to the C-terminal end of the protein but obtained similar results as above.

    Produced:

    Article Title: C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Article Snippet: The SUMO-His6 tagged C9orf72 expressed as inclusion bodies and the construct with the minimal tag (His6 ) did not express at all in E. coli . .. A selection of bacterial expression strains was tried (such as Shuffle T7 express and Nico21(DE3) (from New England Biolabs, Hitchin, UK), Rosetta strains, Rosettagami strains (from Novagen, Watford, UK), SoluBL21 (from Amsbio, Abingdon, UK)), along with a variety of media types and expression trials varying different parameters, but none managed to improve expression levels or produced protein that was stable. .. We also prepared constructs where maltose-binding protein (MBP) and GST tags were moved to the C-terminal end of the protein but obtained similar results as above.

    Bicinchoninic Acid Protein Assay:

    Article Title: Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
    Article Snippet: Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells.

    Synthesized:

    Article Title: Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
    Article Snippet: Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
    Article Snippet: Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells. .. Diadenosine polyphosphates (Ap < sub > n < /sub > As) such as diadenosine tri- and tetraphosphates are formed in prokaryotic as well as eukaryotic cells.

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  • 99
    New England Biolabs nico21
    Nico21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nico21/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nico21 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

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