microcentrifuge tube  (New England Biolabs)


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  • 99
    Name:
    NEB Tube Opener
    Description:
    NEB Tube Opener 2 units
    Catalog Number:
    C1008S
    Price:
    21
    Category:
    Decapping Tools
    Size:
    2 units
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    Structured Review

    New England Biolabs microcentrifuge tube
    NEB Tube Opener
    NEB Tube Opener 2 units
    https://www.bioz.com/result/microcentrifuge tube/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays"

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays

    Journal: bioRxiv

    doi: 10.1101/2021.03.15.435517

    Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.
    Figure Legend Snippet: Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.

    Techniques Used: Irradiation, Modification, Magnetic Beads

    2) Product Images from "Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays"

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays

    Journal: bioRxiv

    doi: 10.1101/2021.03.15.435517

    Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.
    Figure Legend Snippet: Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.

    Techniques Used: Irradiation, Modification, Magnetic Beads

    Related Articles

    Ligation:

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays
    Article Snippet: The ligation reaction was incubated at 25 °C for 1 hour, and T4 DNA ligase was inactivated by incubation at 65 °C for 10 minutes. .. After the ligation step was completed, the master mix was transferred to a 1.7 ml microcentrifuge tube and mixed with 26.5 μl/sample volume of 2X Binding Buffer (20 mM Tris-HCl, (pH 7.5), 600 mM NaCl, 0.2% Triton X-100). .. Pre-equilibrated streptavidin-coated magnetic beads were placed on a magnet stand and the Binding Buffer used for bead storage was removed.

    Binding Assay:

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays
    Article Snippet: The ligation reaction was incubated at 25 °C for 1 hour, and T4 DNA ligase was inactivated by incubation at 65 °C for 10 minutes. .. After the ligation step was completed, the master mix was transferred to a 1.7 ml microcentrifuge tube and mixed with 26.5 μl/sample volume of 2X Binding Buffer (20 mM Tris-HCl, (pH 7.5), 600 mM NaCl, 0.2% Triton X-100). .. Pre-equilibrated streptavidin-coated magnetic beads were placed on a magnet stand and the Binding Buffer used for bead storage was removed.

    Lysis:

    Article Title: Establishment of chromatin accessibility by the conserved transcription factor Grainy head is developmentally regulated
    Article Snippet: Under a dissecting microscope, a microcapillary tube was used to homogenize the embryo. .. The cap was placed into a 1.5 mL microcentrifuge tube containing an additional 40 µL of cold lysis buffer. .. ATAC-seq library preparation was performed using the Illumina Nextera DNA library preparation kit.

    Article Title: GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo
    Article Snippet: .. Briefly, a single dechorionated embryo was transferred to the detached cap of a 1.5 ml microcentrifuge tube containing 10 µl of ice-cold ATAC lysis buffer (10 mM Tris pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40). .. Under a dissecting microscope, a microcapillary tube was used to homogenize the embryo.

    other:

    Article Title: Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
    Article Snippet: Bands in the 20-40 bp range were excised, and each was placed in a 0.5 ml microcentrifuge tube with a small hole in the bottom.

    Article Title: A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis
    Article Snippet: Ovaries were dissected in PBS and transferred to a microcentrifuge tube in liquid nitrogen.

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays
    Article Snippet: Following extension, the reactions were transferred to a pre-chilled 1.7 ml microcentrifuge tube, placed on a magnet stand, and the supernatant was removed.

    Article Title: Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase
    Article Snippet: Samples were centrifuged for 2 min at full speed and transferred to a new microcentrifuge tube.

    Article Title: Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System
    Article Snippet: The contents of the petri dish were passed through a new cell strainer and the samples were then transferred to a microcentrifuge tube and were washed 2x with D-PBS+BSA.

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  • 99
    New England Biolabs microcentrifuge tube
    Verification of 5’ PC Biotin photocleavage using a custom 365 nm <t>microcentrifuge</t> tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.
    Microcentrifuge Tube, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcentrifuge tube/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcentrifuge tube - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.

    Journal: bioRxiv

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays

    doi: 10.1101/2021.03.15.435517

    Figure Lengend Snippet: Verification of 5’ PC Biotin photocleavage using a custom 365 nm microcentrifuge tube irradiator. (A) Denaturing gel showing efficient 365 nm UV-dependent release of a 5’ PC biotin-modified oligonucleotide from two concentrations of streptavidin-coated magnetic beads. (B) Custom built 365 nm tube irradiator.

    Article Snippet: After the ligation step was completed, the master mix was transferred to a 1.7 ml microcentrifuge tube and mixed with 26.5 μl/sample volume of 2X Binding Buffer (20 mM Tris-HCl, (pH 7.5), 600 mM NaCl, 0.2% Triton X-100).

    Techniques: Irradiation, Modification, Magnetic Beads