mgcl2  (New England Biolabs)


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  • 97
    Name:
    Magnesium Chloride MgCl2 Solution
    Description:
    Magnesium Chloride MgCl2 Solution 6 0 ml
    Catalog Number:
    B9021S
    Price:
    23
    Category:
    General Chemical Solutions
    Size:
    6 0 ml
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    Structured Review

    New England Biolabs mgcl2
    Magnesium Chloride MgCl2 Solution
    Magnesium Chloride MgCl2 Solution 6 0 ml
    https://www.bioz.com/result/mgcl2/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Rapid Identification of Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1"

    Article Title: Rapid Identification of Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1

    Journal: Journal of Insect Science

    doi: 10.1093/jisesa/iev137

    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.
    Figure Legend Snippet: Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Techniques Used: Produced, Modification, Amplification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Limited reverse transcriptase activity of phi29 DNA polymerase
    Article Snippet: .. The RCA monomers were first tagged with Illumina adapter sequences during a PCR reaction, containing 1 × Taq DNA polymerase buffer (NEB), 1.5 mM MgCl2 (NEB), 250 μM dNTP, 1 × SYBR Gold, 25 mU/μl Taq DNA polymerase (NEB), 0.5 μM forward primer PE1 , 0.5 μM reverse primer PE2 ( ) and final concentration of 10 pM RCA monomers. ..

    Article Title: Toxin-associated and other genes in Clostridium perfringens type A isolates from bovine clostridial abomasitis (BCA) and jejunal hemorrhage syndrome (JHS)
    Article Snippet: Clostridium perfringens F262 was included as a positive control and PCR water was used for the no-template control. .. Each 25-μL PCR reaction mixture contained 2.5 μL of 10× buffer with magnesium chloride (MgCl2 ) (New England Biolabs, Pickering, Ontario), 2.0 μL of 2.5 mM deoxynucleotide solution mix (dNTP) (New England Biolabs), 1.0 μL of forward primer ( ) (10 pmol/μL), 1.0 μL of reverse primer ( ) (10 pmol/μL), 0.5 μL of Taq polymerase [5U/μL] (New England Biolabs), 13 μL of sterile PCR grade water, and 5 μL of cell lysate. .. Polymerase chain reaction (PCR) was performed on a Biometra T-gradient thermocycler (MBI, Dorval, Montréal, Québec) using touchdown PCR programs.

    Article Title: A DNA aptamer for binding and inhibition of DNA methyltransferase 1
    Article Snippet: Tris base, EDTA and ethanol (EtOH) was obtained from Acros Organics, Promega and Decon Labs, respectively. .. The 5× Taq polymerase and the 25-mM magnesium chloride (MgCl2 ) solution used for PCR were from New England Biolabs. .. The ssDNAs, including the random libraries used for SELEX, the anti-IgE aptamer, as well as those found binding to DNMT1 ( ) were attained from Integrated DNA Technologies, Inc. Beta-casein, cytochrome C, ovalbumin, and hemoglobin were purchased from Sigma-Aldrich.

    Article Title: Group-Specific Multiplex PCR Detection Systems for the Identification of Flying Insect Prey
    Article Snippet: Part of the 18 s rDNA was amplified and sequenced using the primers 18sL0001 and 18sR1100 . .. Each 10 µl reaction mix contained 1.5 µl DNA extract (typically 2–200 ng DNA), 5 µg bovine serum albumin (BSA), 0.5 µM of each primer, 0.2 mM dNTP (Genecraft, Köln, Germany), 1× reaction buffer (NEB, Ipswich, U.S.), 3 mM MgCl2 (NEB), 0.25 U oneTAQ (NEB), and PCR grade water to adjust the volume. .. Cycling conditions in an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany) where 2 min at 94°C, 35 cycles of 20 s at 94°C, 30 s at 50°C, 90 s at 68°C and final elongation for 3 min at 68°C.

    Article Title: A new phylogeny-based tribal classification of subfamily Detarioideae, an early branching clade of florally diverse tropical arborescent legumes
    Article Snippet: Three plastid (matK -trnK , rpL16 and trnG -trnG2G ) regions and the nuclear ribosomal internal transcribed spacers (ITS/5.8 S) were amplified and sequenced. .. The PCR amplification mix in reaction volumes of 50 μL contained 4 units of Taq DNA polymerase, 1× Taq DNA polymerase buffer with 1.5 mmol MgCl2 (New England Biolabs, Pickering, Ontario, Canada), 200 μmol/L of each dNTP (Fermentas, Burlington, Ontario, Canada), 3 μmol/L of each primer, and 50–100 ng of genomic DNA. .. For recalcitrant samples, BSA (0.1 μg/μL, New England BioLabs, Ipswich, Mass.), Tween 20 (0.03%, J-T. Baker, Phillipsburg, New Jersey, USA), and pure DMSO (4%, Fisher Scientific, Ottawa, Ontario, Canada) were added to the mix.

    Concentration Assay:

    Article Title: Limited reverse transcriptase activity of phi29 DNA polymerase
    Article Snippet: .. The RCA monomers were first tagged with Illumina adapter sequences during a PCR reaction, containing 1 × Taq DNA polymerase buffer (NEB), 1.5 mM MgCl2 (NEB), 250 μM dNTP, 1 × SYBR Gold, 25 mU/μl Taq DNA polymerase (NEB), 0.5 μM forward primer PE1 , 0.5 μM reverse primer PE2 ( ) and final concentration of 10 pM RCA monomers. ..

    In Vitro:

    Article Title: The Phosphorylation Status of the Serine-Rich Region of the Human Cytomegalovirus 86-Kilodalton Major Immediate-Early Protein IE2/IEP86 Affects Temporal Viral Gene Expression
    Article Snippet: Equivalent amounts (1 to 5 μg) of fusion proteins (quantitated by Sypro red staining) were used for in vitro kinase experiments and in vitro binding experiments. .. For in vitro CKII assays, fusion proteins were incubated for 30 min at 30°C with 25 U of CKII (New England Biolabs) in 1× CKII buffer (20 mM Tris-HCl [pH 7.5], 50 mM KCl, and 10 mM MgCl2 ) (New England Biolabs) containing 0.2 mM ATP and 20 μCi of [γ-32 P]ATP. .. The phosphorylated fusion proteins were purified by using gluthatione-Sepharose beads, boiled in SDS-PAGE loading buffer, and separated by SDS-10% PAGE.The gels were stained with Sypro red to quantify the total amounts of protein, dried, and exposed to a PhophorImager screen to quantitate phosphorylation.

    Incubation:

    Article Title: The Phosphorylation Status of the Serine-Rich Region of the Human Cytomegalovirus 86-Kilodalton Major Immediate-Early Protein IE2/IEP86 Affects Temporal Viral Gene Expression
    Article Snippet: Equivalent amounts (1 to 5 μg) of fusion proteins (quantitated by Sypro red staining) were used for in vitro kinase experiments and in vitro binding experiments. .. For in vitro CKII assays, fusion proteins were incubated for 30 min at 30°C with 25 U of CKII (New England Biolabs) in 1× CKII buffer (20 mM Tris-HCl [pH 7.5], 50 mM KCl, and 10 mM MgCl2 ) (New England Biolabs) containing 0.2 mM ATP and 20 μCi of [γ-32 P]ATP. .. The phosphorylated fusion proteins were purified by using gluthatione-Sepharose beads, boiled in SDS-PAGE loading buffer, and separated by SDS-10% PAGE.The gels were stained with Sypro red to quantify the total amounts of protein, dried, and exposed to a PhophorImager screen to quantitate phosphorylation.

    Amplification:

    Article Title: A new phylogeny-based tribal classification of subfamily Detarioideae, an early branching clade of florally diverse tropical arborescent legumes
    Article Snippet: Three plastid (matK -trnK , rpL16 and trnG -trnG2G ) regions and the nuclear ribosomal internal transcribed spacers (ITS/5.8 S) were amplified and sequenced. .. The PCR amplification mix in reaction volumes of 50 μL contained 4 units of Taq DNA polymerase, 1× Taq DNA polymerase buffer with 1.5 mmol MgCl2 (New England Biolabs, Pickering, Ontario, Canada), 200 μmol/L of each dNTP (Fermentas, Burlington, Ontario, Canada), 3 μmol/L of each primer, and 50–100 ng of genomic DNA. .. For recalcitrant samples, BSA (0.1 μg/μL, New England BioLabs, Ipswich, Mass.), Tween 20 (0.03%, J-T. Baker, Phillipsburg, New Jersey, USA), and pure DMSO (4%, Fisher Scientific, Ottawa, Ontario, Canada) were added to the mix.

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  • 97
    New England Biolabs mgcl2
    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM <t>MgCl2</t> (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.
    Mgcl2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl2/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

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    Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Journal: Journal of Insect Science

    Article Title: Rapid Identification of Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1

    doi: 10.1093/jisesa/iev137

    Figure Lengend Snippet: Derivative dissociation plots produced by different reagents using one microliter of lysate produced by homogenizing one H. armigera leg with 24 H. zea legs in 625 μl of modified squish buffer. (A) Amplification reactions made with KAPA SYBR Fast Master Mix (purple) and NEB LongAmp Taq polymerase and buffer with 2.5 mM MgCl2 (Red). Peaks representing 18S rRNA control amplicon and species-specific amplicons of H. armigera and H. zea are present in all reactions, but melting temperatures were different in two reagents. (B) An enlarged view of the derivative dissociation plot produced by amplification reactions with NEB LongAmp Taq polymerase and buffer. Although variations in dissociation plots between samples were observed, the 3-peak dissociation plot was clearly identifiable.

    Article Snippet: LongAmp Taq polymerase and reaction buffer with 2.5 mM MgCl2 (New England Biolabs) produced dissociation curves with lower intensity than ready-to-use master mixes but sufficient to detect H. armigera in 1:24 ratio of H. armigera : H. zea tissue lysate ( a and b).

    Techniques: Produced, Modification, Amplification