soc medium  (New England Biolabs)


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  • 95
    Name:
    SOC Outgrowth Medium
    Description:
    SOC Outgrowth Medium 100 ml
    Catalog Number:
    b9020s
    Price:
    84
    Size:
    100 ml
    Category:
    Yeast Media
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    Structured Review

    New England Biolabs soc medium
    SOC Outgrowth Medium
    SOC Outgrowth Medium 100 ml
    https://www.bioz.com/result/soc medium/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    soc medium - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: The linearized vector, the 3′ and 5′ target gene fragments and the kanR gene were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the assembled knockout vector was transformed into NEB 5-alpha competent E. coli. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C. .. Plasmid DNA form positively identified bacterial clones was purified and verified by DNA sequencing with primers pHY304-Ver-F and pHY304-Ver-R. Electrocompetent GAS cells were prepared as follows: overnight cultures of M1 5448 supplemented with 20 mM glycine (Spectrum Laboratory Products, Inc.) were back-diluted (1:20) in 150 mL of THY + 20 mM glycine, incubated until they reached OD600 = 0.2–0.4, and cooled on ice for 30 minutes.

    Amplification:

    Article Title: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
    Article Snippet: After incubation with SOC outgrowth medium (New England Biolabs) for 1 hour the bacteria were plated on agar plates containing both spectinomycin and kanamycin along with ATc and arabinose inducers. .. The same region around the protospacer in the pre-selection library and the post-selection DNA was then amplified separately using NEBNext High-Fidelity PCR Polymerase (New England Biolabs) with flanking HTS primer pairs listed in the .

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants. ..

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The assembly product was used as a template for amplification of the entire DNA region with primer pair ess-cat -F/ess-cat -R. Plasmid pHY304 ( ) was PCR amplified using primers pHY304-F and pPHY304-R. PCR products were purified, subjected to digestion with EagI-HF and HindIII-HF restriction enzymes (New England BioLabs), purified, and ligated using Quick Ligation Kit (New England BioLabs). .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
    Article Snippet: Paragraph title: SgRNA plasmid amplification ... One Shot Stbl3 chemically competent E. coli (Thermo Fisher, cat. no. C737303) SOC outgrowth medium (New England BioLabs, cat. no. B9020S) NucleoBond Xtra Midi EF (Macherey-Nagel, cat. no. 740420.50) Critical An endotoxin-free plasmid purification kit is important for avoiding endotoxicity in virus production and mammalian cell culture.

    Depletion Assay:

    Article Title: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
    Article Snippet: Paragraph title: PAM depletion assay ... After incubation with SOC outgrowth medium (New England Biolabs) for 1 hour the bacteria were plated on agar plates containing both spectinomycin and kanamycin along with ATc and arabinose inducers.

    Construct:

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: The constructs were transformed into rubidium chloride-treated competent E. coli DH10B cells using heat shock at 42 °C for 1 min, followed by another 2-min incubation on ice. .. Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm.

    SYBR Green Assay:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Incubation:

    Article Title: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
    Article Snippet: .. After incubation with SOC outgrowth medium (New England Biolabs) for 1 hour the bacteria were plated on agar plates containing both spectinomycin and kanamycin along with ATc and arabinose inducers. .. After incubating overnight, the bacterial cells on the agar plates were scraped and the plasmids extracted using the ZymoPURE Plasmid Midiprep Kit (Zymo Research).

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants. ..

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C. .. Bacterial colonies were passaged onto fresh solid medium and screened for the presence of pHY304-ess ::cat by PCR reaction with primers pHY304-Ver-F and pHY304-Ver-R (each primer is complementary to the vector region approximately 200 base pairs up- and downstream respectively from the insertion site.

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: The constructs were transformed into rubidium chloride-treated competent E. coli DH10B cells using heat shock at 42 °C for 1 min, followed by another 2-min incubation on ice. .. Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm.

    Expressing:

    Article Title: Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
    Article Snippet: Metagenomic selection for SpyCas9 antagonists In the first iteration of SpyCas9 selection, 360 ng of each metagenomic library was electroporated into a strain of ultra-competent E. coli (NEB Turbo) containing the spectinomycin-marked SpyCas9 expression plasmid (pSpyCas9). .. Following electroporation, cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S).

    Transformation Assay:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: E. coli transformation: One microliter of assembly solution was mixed with 17 µL of E. coli Dh5α heat-shock competent cell (C2987H, NEB) in a pre-chilled 1.7 mL tube on ice (Axygen). .. The tube was heat-shocked in a 42 °C water bath for exactly 35 s and was quenched on ice for 2 min The cell solution was mixed with 150 µL of SOC medium (B9020S, NEB) and directly plated on LB Agar plate that contained a proper antibiotic.

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: The linearized vector, the 3′ and 5′ target gene fragments and the kanR gene were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the assembled knockout vector was transformed into NEB 5-alpha competent E. coli. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The entire ligation product was used for transformation of E. coli NEB Turbo competent cells (New England BioLabs), according to the manufacturer’s recommendation. .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: The constructs were transformed into rubidium chloride-treated competent E. coli DH10B cells using heat shock at 42 °C for 1 min, followed by another 2-min incubation on ice. .. Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm.

    Electroporation:

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants. ..

    Article Title: Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
    Article Snippet: .. Following electroporation, cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S). ..

    Transfection:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Ligation:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: RE-digestion and ligation used in Supplementary Fig. were similar to the above except that BamHI (R0136S, NEB) and AatII (R0117S, NEB) were used. .. The tube was heat-shocked in a 42 °C water bath for exactly 35 s and was quenched on ice for 2 min The cell solution was mixed with 150 µL of SOC medium (B9020S, NEB) and directly plated on LB Agar plate that contained a proper antibiotic.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The entire ligation product was used for transformation of E. coli NEB Turbo competent cells (New England BioLabs), according to the manufacturer’s recommendation. .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Protease Inhibitor:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Cell Culture:

    Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
    Article Snippet: .. One Shot Stbl3 chemically competent E. coli (Thermo Fisher, cat. no. C737303) SOC outgrowth medium (New England BioLabs, cat. no. B9020S) NucleoBond Xtra Midi EF (Macherey-Nagel, cat. no. 740420.50) Critical An endotoxin-free plasmid purification kit is important for avoiding endotoxicity in virus production and mammalian cell culture. .. Other endotoxin-free plasmid purification kits, such as the Qiagen Plasmid Plus Maxi kit, may be used as substitutes.

    Generated:

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants. ..

    DNA Sequencing:

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C. .. Plasmid DNA form positively identified bacterial clones was purified and verified by DNA sequencing with primers pHY304-Ver-F and pHY304-Ver-R. Electrocompetent GAS cells were prepared as follows: overnight cultures of M1 5448 supplemented with 20 mM glycine (Spectrum Laboratory Products, Inc.) were back-diluted (1:20) in 150 mL of THY + 20 mM glycine, incubated until they reached OD600 = 0.2–0.4, and cooled on ice for 30 minutes.

    Sequencing:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: The tube was heat-shocked in a 42 °C water bath for exactly 35 s and was quenched on ice for 2 min The cell solution was mixed with 150 µL of SOC medium (B9020S, NEB) and directly plated on LB Agar plate that contained a proper antibiotic. .. Colony PCR and Sanger sequencing were carried out to determine assembly efficiency and sequencing accuracy of DNA assembly.

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm. .. The resulting interference plasmids were PCR-screened in E. coli ) for the presence of the insert and sequenced to confirm sequence content.

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: Plasmids were extracted from single colonies and the correct construction of the knockout vector was confirmed by Sanger sequencing using the psk-5-TLP12-F and psk-3-TLP12-R primers. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Binding Assay:

    Article Title: Rational library design by functional CDR resampling
    Article Snippet: E. coli maltose binding protein (MBP) was produced in house. .. Turbo Competent E. coli (High Efficiency) cells (C2984I) and SOC media (B9020S) were purchased from New England Biolabs.

    Electro Cell Manipulation:

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: The knockout vector was electroporated into C. jejuni A17 wild type competent cells using an ECM600 Electro Cell Manipulator (BTX) with the following settings: resistance – 2.5 kV, capacitance timing – 25 μF and resistance timing – 186 Ω. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Isolation:

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: Chromosomal DNA of M1 5448 was isolated and served as a template for PCR amplification of 500 base pair regions directly up- and downstream of the ess locus using primer pairs ess -up-F/ess -up-R and ess -down-F/ess -down-R. .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Flow Cytometry:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Labeling:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Purification:

    Article Title: Rational library design by functional CDR resampling
    Article Snippet: Biotinylated peptides antigens were designed in-house and purchased from Biopeptik with 90% purity (peptides: XIAP1B (Biotin-SGSPVSASTLARAGFLYTGE, Human), XIAP2B (Biotin-THADYLLRTGQVVDISDTIY, Human), GRAP2B (Biotin-SLNKLVDYYRTNSISRQKQI, Human), GRAP3B (Biotin-TDPVQLQAAGRVRWARALYD, Human), LMNA3B (Biotin-DEYQELLDIKLALDMEIHAYRK, Human), TDP_42_NLS (Biotin- PKD NKRKMDETDASSAVKVKRA), XIAP3B (Biotin-AEAVDKCPMCYTVITFKQK, Human), LMNA2B (Biotin-RIDSLSAQLSQLQKQLAAKE, Human) purified proteins were provided internally by Abcam (proteins: hIL-12p70( / , Human), FGF basic protein ( , Human), Dtk-Fc( , Human), BCMA-Fc(A7KBT3, Human), Nogo Fc( , Human), IL-13( , Human), myelin basic protein( , Human), hLeptin , IFN alpha2( , Human), BDNF( ,Human), IL-1 beta( , Human), IL3 beta( , Human). .. Turbo Competent E. coli (High Efficiency) cells (C2984I) and SOC media (B9020S) were purchased from New England Biolabs.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The assembly product was used as a template for amplification of the entire DNA region with primer pair ess-cat -F/ess-cat -R. Plasmid pHY304 ( ) was PCR amplified using primers pHY304-F and pPHY304-R. PCR products were purified, subjected to digestion with EagI-HF and HindIII-HF restriction enzymes (New England BioLabs), purified, and ligated using Quick Ligation Kit (New England BioLabs). .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
    Article Snippet: .. One Shot Stbl3 chemically competent E. coli (Thermo Fisher, cat. no. C737303) SOC outgrowth medium (New England BioLabs, cat. no. B9020S) NucleoBond Xtra Midi EF (Macherey-Nagel, cat. no. 740420.50) Critical An endotoxin-free plasmid purification kit is important for avoiding endotoxicity in virus production and mammalian cell culture. .. Other endotoxin-free plasmid purification kits, such as the Qiagen Plasmid Plus Maxi kit, may be used as substitutes.

    Polymerase Chain Reaction:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: The tube was heat-shocked in a 42 °C water bath for exactly 35 s and was quenched on ice for 2 min The cell solution was mixed with 150 µL of SOC medium (B9020S, NEB) and directly plated on LB Agar plate that contained a proper antibiotic. .. Colony PCR and Sanger sequencing were carried out to determine assembly efficiency and sequencing accuracy of DNA assembly.

    Article Title: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
    Article Snippet: After incubation with SOC outgrowth medium (New England Biolabs) for 1 hour the bacteria were plated on agar plates containing both spectinomycin and kanamycin along with ATc and arabinose inducers. .. The same region around the protospacer in the pre-selection library and the post-selection DNA was then amplified separately using NEBNext High-Fidelity PCR Polymerase (New England Biolabs) with flanking HTS primer pairs listed in the .

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The assembly product was used as a template for amplification of the entire DNA region with primer pair ess-cat -F/ess-cat -R. Plasmid pHY304 ( ) was PCR amplified using primers pHY304-F and pPHY304-R. PCR products were purified, subjected to digestion with EagI-HF and HindIII-HF restriction enzymes (New England BioLabs), purified, and ligated using Quick Ligation Kit (New England BioLabs). .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm. .. The resulting interference plasmids were PCR-screened in E. coli ) for the presence of the insert and sequenced to confirm sequence content.

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: The PCRBio HiFi Kit was used for all PCRs and PCR reactions were put together as per manufacturer’s recommendations with 1 U of PCRBIO HIFI polymerase, 400 nM forward and reverse primers and ~ 500 ng of genomic template DNA in 50 μl reactions. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Protein Extraction:

    Article Title: Rational library design by functional CDR resampling
    Article Snippet: Turbo Competent E. coli (High Efficiency) cells (C2984I) and SOC media (B9020S) were purchased from New England Biolabs. .. B-Per Bacterial Protein Extraction Reagent (78248) was purchased from Pierce.

    Plasmid Preparation:

    Article Title: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
    Article Snippet: The second plasmid contains the target protospacer and a kanamycin resistance gene. .. After incubation with SOC outgrowth medium (New England Biolabs) for 1 hour the bacteria were plated on agar plates containing both spectinomycin and kanamycin along with ATc and arabinose inducers.

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: The linearized vector, the 3′ and 5′ target gene fragments and the kanR gene were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the assembled knockout vector was transformed into NEB 5-alpha competent E. coli. .. After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: The assembly product was used as a template for amplification of the entire DNA region with primer pair ess-cat -F/ess-cat -R. Plasmid pHY304 ( ) was PCR amplified using primers pHY304-F and pPHY304-R. PCR products were purified, subjected to digestion with EagI-HF and HindIII-HF restriction enzymes (New England BioLabs), purified, and ligated using Quick Ligation Kit (New England BioLabs). .. As pHY304 contains a thermosensitive origin of replication that replicates at 30°C, the outgrowth of bacteria in SOC medium (New England BioLabs) and overnight incubation on solid medium supplemented with erythromycin was performed at 30°C.

    Article Title: Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
    Article Snippet: Metagenomic selection for SpyCas9 antagonists In the first iteration of SpyCas9 selection, 360 ng of each metagenomic library was electroporated into a strain of ultra-competent E. coli (NEB Turbo) containing the spectinomycin-marked SpyCas9 expression plasmid (pSpyCas9). .. Following electroporation, cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S).

    Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus
    Article Snippet: The pTRKH2 plasmid , a replicating shuttle vector for E. coli and Lactobacillus ). .. Cells were recovered in 900 μL of SOC medium (NEB) at 37 °C aerobically for 3 h and then plated on BHI with 150 μg⋅mL−1 Erm.

    Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
    Article Snippet: .. One Shot Stbl3 chemically competent E. coli (Thermo Fisher, cat. no. C737303) SOC outgrowth medium (New England BioLabs, cat. no. B9020S) NucleoBond Xtra Midi EF (Macherey-Nagel, cat. no. 740420.50) Critical An endotoxin-free plasmid purification kit is important for avoiding endotoxicity in virus production and mammalian cell culture. .. Other endotoxin-free plasmid purification kits, such as the Qiagen Plasmid Plus Maxi kit, may be used as substitutes.

    Selection:

    Article Title: Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
    Article Snippet: Paragraph title: Metagenomic selection for SpyCas9 antagonists ... Following electroporation, cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S).

    In Vitro:

    Article Title: Enabling Biocatalysis by High-Throughput Protein Engineering Using Droplet Microfluidics Coupled to Mass Spectrometry
    Article Snippet: .. PURExpress In Vitro Protein Synthesis Kit, RNAse inhibitor, and SOC outgrowth medium were from New England BioLabs (Ipswich, MA). ..

    Knock-Out:

    Article Title: The transducer-like protein Tlp12 of Campylobacter jejuni is involved in glutamate and pyruvate chemotaxis
    Article Snippet: Paragraph title: Δtlp12 knockout generation ... After electroporation, 100 μl SOC Outgrowth Medium (NEB) was added to the mixture which was then inoculated onto COS plates and incubated for 16 h at 37 °C under microaerophilic conditions to encourage the amplification of the generated mutants.

    Produced:

    Article Title: Rational library design by functional CDR resampling
    Article Snippet: E. coli maltose binding protein (MBP) was produced in house. .. Turbo Competent E. coli (High Efficiency) cells (C2984I) and SOC media (B9020S) were purchased from New England Biolabs.

    Gel Extraction:

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. Reagents 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), C29871); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, P20270); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, BP267110); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, P36930). ..

    Article Title: Structural Basis for Influenza Virus NS1 Protein Block of mRNA Nuclear Export
    Article Snippet: .. 50xAdvantage Polymerase mix (Clontech (EMD), 639202); dNTP’s (Clontech (EMD), 639125); BamHI-HF (New England Biolabs (NEB), R3136T); NotI (New England Biolabs (NEB), R0189S); SmaI (New England Biolabs (NEB), R0141); XhoI (New England Biolabs (NEB), R0146S); T4 DNA Ligase (New England Biolabs (NEB), M0202); QIAquick Gel Extraction Kit (Qiagen, 28704); NEB® 5-alpha Competent E. coli (New England Biolabs (NEB), ); Rosetta (DE3) Competent Cells (EMD Millipore, 70954); SOC Outgrowth Medium (New England Biolabs (NEB), B9020); QIAprep Spin Miniprep Kit (250) (Qiagen, 27106); Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150); RNeasy Plus Mini Kit (Qiagen, 74134); Random Hexamers (50 μM) (Thermo Fisher Scientific, N8080127); Protector RNase Inhibitor (Roche, 03335402001); SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, 18064014); LIGHTCYCLER 480 SYBR GREEN I MASTER (Roche, 04707516001); LightCycler® 480 Multiwell Plate 96, White (Roche, 04729692001); NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833); Complete EDTA-free protease inhibitor tablets (Sigma-Aldrich, 11873580001); TransIT-X2® Dynamic Delivery System (Mirus Bio, MIR6000); SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34096); L-Glutathione reduced (Sigma-Aldrich, G4251–25G); Ampicillin (Sigma-Aldrich, A-9518); Kanamycin Monosulfate (Gold Biotechnology, K-120–10); IPTG (Gold Biotechnology, I2481C50); Imidazole (Sigma-Aldrich, 56750); PMSF (RPI, ); Aprotinin (Santa Cruz Biotechnology, sc-3595); Leupeptin (Santa Cruz Biotechnology, sc295358); Pepstatin A (Thermo Fisher Scientific, ); Glutathione Sepharose 4B (GE Healthcare, 17–0756-01); Amylose Resin (New England Biolabs (NEB), E8021S); Ni-NTA Agarose (Qiagen, 30210); Mono Q 5/50 GL (GE Healthcare, 17-5166-01); HiTrap SP HP (GE Healthcare, 17-1151-01); Superdex 200 HR 10/30 (GE Healthcare, 17-1088-01); EasyTag EXPRESS35 S Protein Labeling Mix, [35S]-, 7mCi (PerkinElmer, NEG772007MC); T7 RiboMAX™ Express Large Scale RNA Production System (Promega, P1320); Protein G Sepharose® 4 Fast Flow (GE Healthcare, 17-0618-01); Hoechst 33258 (Molecular Probes/Life Technologies); M mRNA probes (Biosearch Technologies) ; ProLong Gold antifade reagent (Life Technologies, ). ..

    Hood:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: L-alanine (Sigma-Aldrich, cat. no. A7627) L-arginine (Sigma-Aldrich, cat. no. A5006) L-asparagine (Sigma-Aldrich, cat. no. A8381) L-aspartic acid (Sigma-Aldrich, cat. no. A9256) L-cysteine (Sigma-Aldrich, cat. no. C7352) L-glutamic acid (Sigma-Aldrich, cat. no. G5889) L-glutamine (Sigma-Aldrich, cat. no. G3126) L-histidine (Sigma-Aldrich, cat. no. H8000) L-isoleucine (Sigma-Aldrich, cat. no. I2752) L-leucine (Sigma-Aldrich, cat. no. L8000) L-lysine (Sigma-Aldrich, cat. no. L5626) L-methionine (Sigma-Aldrich, cat. no. M9625) L-phenylalanine (Sigma-Aldrich, cat. no. P2126) L-proline (Sigma-Aldrich, cat. no. P0380) L-serine (Sigma-Aldrich, cat. no. S4500) L-threonine (Sigma-Aldrich, cat. no. T8625) L-tryptophan (Sigma-Aldrich, cat. no. T0254) L-tyrosine (Sigma-Aldrich, cat. no. T3754) L-valine (Sigma-Aldrich, cat. no. V0500) Ammonium sulfate (Sigma-Aldrich, cat. no. A4418) Yeast peptone (Sigma-Aldrich, cat. no. 39396) Yeast extract (EMD Chemicals, cat. no. 1.03753) Yeast Nitrogen Base (without amino acids, without ammonium sulfate) (Sigma-Aldrich, cat. no. 51483) Salmon sperm DNA, 10 mg/ml (Sigma-Aldrich, cat. no. D7656) Cycloheximide (Sigma-Aldrich, cat. no. C7698) Adenine (Sigma-Aldrich, cat. no. A3159) Uracil (Sigma-Aldrich, cat. no. U0750) SOC medium (New England BioLabs, cat. no. B9020S) 3-AT (3-Amino-1,2,4-triazole) (Sigma-Aldrich, cat. no. A8056). .. L-alanine (Sigma-Aldrich, cat. no. A7627) L-arginine (Sigma-Aldrich, cat. no. A5006) L-asparagine (Sigma-Aldrich, cat. no. A8381) L-aspartic acid (Sigma-Aldrich, cat. no. A9256) L-cysteine (Sigma-Aldrich, cat. no. C7352) L-glutamic acid (Sigma-Aldrich, cat. no. G5889) L-glutamine (Sigma-Aldrich, cat. no. G3126) L-histidine (Sigma-Aldrich, cat. no. H8000) L-isoleucine (Sigma-Aldrich, cat. no. I2752) L-leucine (Sigma-Aldrich, cat. no. L8000) L-lysine (Sigma-Aldrich, cat. no. L5626) L-methionine (Sigma-Aldrich, cat. no. M9625) L-phenylalanine (Sigma-Aldrich, cat. no. P2126) L-proline (Sigma-Aldrich, cat. no. P0380) L-serine (Sigma-Aldrich, cat. no. S4500) L-threonine (Sigma-Aldrich, cat. no. T8625) L-tryptophan (Sigma-Aldrich, cat. no. T0254) L-tyrosine (Sigma-Aldrich, cat. no. T3754) L-valine (Sigma-Aldrich, cat. no. V0500) Ammonium sulfate (Sigma-Aldrich, cat. no. A4418) Yeast peptone (Sigma-Aldrich, cat. no. 39396) Yeast extract (EMD Chemicals, cat. no. 1.03753) Yeast Nitrogen Base (without amino acids, without ammonium sulfate) (Sigma-Aldrich, cat. no. 51483) Salmon sperm DNA, 10 mg/ml (Sigma-Aldrich, cat. no. D7656) Cycloheximide (Sigma-Aldrich, cat. no. C7698) Adenine (Sigma-Aldrich, cat. no. A3159) Uracil (Sigma-Aldrich, cat. no. U0750) SOC medium (New England BioLabs, cat. no. B9020S) 3-AT (3-Amino-1,2,4-triazole) (Sigma-Aldrich, cat. no. A8056).

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  • 95
    New England Biolabs soc medium
    Soc Medium, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 3 article reviews
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    soc medium - by Bioz Stars, 2020-02
    95/100 stars
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