t4 dna ligase  (New England Biolabs)


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  • 99
    Name:
    T4 DNA Ligase
    Description:
    T4 DNA Ligase 100 000 units
    Catalog Number:
    m0202l
    Price:
    256
    Size:
    100 000 units
    Category:
    DNA Ligases
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    Structured Review

    New England Biolabs t4 dna ligase
    T4 DNA Ligase
    T4 DNA Ligase 100 000 units
    https://www.bioz.com/result/t4 dna ligase/product/New England Biolabs
    Average 99 stars, based on 6407 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Produced:

    Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL
    Article Snippet: .. While wild type RNase H1 together with T4 DNA ligase produced a ligated product, negligible ligation was seen with the mutant proteins (Figure ). .. Longer extension products were formed with the V142I mutant compared to A185V; this can be explained by V142I being able to remove some ribonucleotides (Figure , compare lanes 7–9 with lanes 12–14) and thus making a longer gap for POLγ to fill.

    Mutagenesis:

    Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL
    Article Snippet: .. While wild type RNase H1 together with T4 DNA ligase produced a ligated product, negligible ligation was seen with the mutant proteins (Figure ). .. Longer extension products were formed with the V142I mutant compared to A185V; this can be explained by V142I being able to remove some ribonucleotides (Figure , compare lanes 7–9 with lanes 12–14) and thus making a longer gap for POLγ to fill.

    Incubation:

    Article Title: The yeast high mobility group protein HMO2, a subunit of the chromatin-remodeling complex INO80, binds DNA ends
    Article Snippet: .. One hundred nanograms of linearized pGEM5 was incubated with varying concentrations of HMO2 or HMO1 at room temperature for 1 h. To this reaction, 1 μl of 400 U/μl of T4 DNA ligase was added and incubated at room temperature for 1 h. Samples were treated with exonuclease III (100 U/μl) at room temperature for 1 h. Reactions were terminated by adding 2 μl stop buffer (5 mM EDTA, 1.1% glycerol and 0.2 mg/ml proteinase K). ..

    other:

    Article Title: The yeast high mobility group protein HMO2, a subunit of the chromatin-remodeling complex INO80, binds DNA ends
    Article Snippet: Consistent with the inability of HMO2 to protect DNA with BspHI-generated 5′ overhangs, such DNA may be re-ligated in presence of T4 DNA ligase ( B, lane 3; lanes 1 and 2 contain DNA without and with T4 DNA ligase, respectively).

    Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL
    Article Snippet: For this, we performed the POLRMT-primed minicircle assay with T4 DNA ligase, and then tested whether the closed circular products could be subsequently converted to nicked circles by KOH treatment ( ).

    Article Title: A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4
    Article Snippet: Incision that is not coincident with the junction branch point generates a small gapped region in the incision product, confirmed by the inability to ligate the resulting duplex with T4 DNA ligase.

    Ligation:

    Article Title: Efficient assembly of very short oligonucleotides using T4 DNA Ligase
    Article Snippet: .. Ligation assays 25 μL ligations contained 0.2 μM immobilized dsDNA on beads, 0.002 to 20 μM 5'-phosphorylated, 3'-FAM oligo, 20 units of T4 DNA Ligase (NEB), 1× T4 DNA Ligase Buffer (50 mM Tris-HCl, 10 mM MgCl2 , 1 mM ATP, 10 mM Dithiothreitol pH 7.5 @ 25°C). ..

    Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL
    Article Snippet: .. While wild type RNase H1 together with T4 DNA ligase produced a ligated product, negligible ligation was seen with the mutant proteins (Figure ). .. Longer extension products were formed with the V142I mutant compared to A185V; this can be explained by V142I being able to remove some ribonucleotides (Figure , compare lanes 7–9 with lanes 12–14) and thus making a longer gap for POLγ to fill.

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    https://www.bioz.com/result/soc outgrowth medium/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
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    soc outgrowth medium - by Bioz Stars, 2020-08
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