soc outgrowth medium  (New England Biolabs)


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  • 99
    Name:
    SOC Outgrowth Medium
    Description:
    SOC Outgrowth Medium 100 ml
    Catalog Number:
    B9020S
    Price:
    84
    Category:
    Yeast Media
    Size:
    100 ml
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    Structured Review

    New England Biolabs soc outgrowth medium
    SOC Outgrowth Medium
    SOC Outgrowth Medium 100 ml
    https://www.bioz.com/result/soc outgrowth medium/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soc outgrowth medium - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Genomically Mined Acoustic Reporter Genes Enable On-Demand In Vivo Monitoring of Tumor-Homing Bacteria
    Article Snippet: In vitro bacterial expression of ARGs For initial testing, all constructs were expressed in BL21(DE3) E. coli (NEB). .. Fifty μL of electrocompetent E. coli were transformed with 1.5 μL of purified plasmid DNA (Econospin 96-well filter plate, Epoch Life Science), and 1 mL of SOC medium (NEB) was added immediately after electroporation. .. These cultures were incubated at 37°C for 2 hr, and 150 uL was inoculated into larger 1.5 mL LB cultures containing 100 ug/mL kanamycin and 1% (w/v) glucose in a deep-well 96-well plate and grown overnight in a shaking incubator at 30°C.

    Purification:

    Article Title: Genomically Mined Acoustic Reporter Genes Enable On-Demand In Vivo Monitoring of Tumor-Homing Bacteria
    Article Snippet: In vitro bacterial expression of ARGs For initial testing, all constructs were expressed in BL21(DE3) E. coli (NEB). .. Fifty μL of electrocompetent E. coli were transformed with 1.5 μL of purified plasmid DNA (Econospin 96-well filter plate, Epoch Life Science), and 1 mL of SOC medium (NEB) was added immediately after electroporation. .. These cultures were incubated at 37°C for 2 hr, and 150 uL was inoculated into larger 1.5 mL LB cultures containing 100 ug/mL kanamycin and 1% (w/v) glucose in a deep-well 96-well plate and grown overnight in a shaking incubator at 30°C.

    Plasmid Preparation:

    Article Title: Genomically Mined Acoustic Reporter Genes Enable On-Demand In Vivo Monitoring of Tumor-Homing Bacteria
    Article Snippet: In vitro bacterial expression of ARGs For initial testing, all constructs were expressed in BL21(DE3) E. coli (NEB). .. Fifty μL of electrocompetent E. coli were transformed with 1.5 μL of purified plasmid DNA (Econospin 96-well filter plate, Epoch Life Science), and 1 mL of SOC medium (NEB) was added immediately after electroporation. .. These cultures were incubated at 37°C for 2 hr, and 150 uL was inoculated into larger 1.5 mL LB cultures containing 100 ug/mL kanamycin and 1% (w/v) glucose in a deep-well 96-well plate and grown overnight in a shaking incubator at 30°C.

    Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
    Article Snippet: .. Rosetta™ 2(DE3)pLysS Singles Competent Cells (Millipore cat. no. 71401–3) TwinStrep-SUMO-LwaCas13a expression plasmid (Addgene: pC013 - Twinstrep-SUMO-huLwCas13a, Plasmid # 90097) SOC Outgrowth Medium 25 mL (New England BioLabs cat. no. B9020S) Luria Broth (LB), Ready-Made Powder 250 g, (Thermo Scientific cat. no. J75854.30 TB Broth powder (VWR™ cat. no. 89126–196) Glycerol ≥99.5% (Sigma-Aldrich cat. no. G9012–1GA) Ampicillin, sodium salt (AmericanBio, cat. no. AB00115–00025; CAS 69–52-3) Isopropyl β-D-1-thiogalactopyranoside (IPTG), Sigma-Aldrich cat. no. I6758 Sodium chloride (Sigma-Aldrich cat. no. S7653) DL-Dithiothreitol solution, BioUltra, for molecular biology, 1 M in H2O (Sigma Aldrich cat. no. 43816–50ML) Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride solution 0.5M, pH 7.0 (Sigma-Aldrich cat. no. 646547–10X1ML) β-Mercaptoethanol (Sigma-Aldrich cat. no. M6250–100ML) CAUTION: β-Mercaptoethanol is considered toxic. ..

    Electroporation:

    Article Title: Genomically Mined Acoustic Reporter Genes Enable On-Demand In Vivo Monitoring of Tumor-Homing Bacteria
    Article Snippet: In vitro bacterial expression of ARGs For initial testing, all constructs were expressed in BL21(DE3) E. coli (NEB). .. Fifty μL of electrocompetent E. coli were transformed with 1.5 μL of purified plasmid DNA (Econospin 96-well filter plate, Epoch Life Science), and 1 mL of SOC medium (NEB) was added immediately after electroporation. .. These cultures were incubated at 37°C for 2 hr, and 150 uL was inoculated into larger 1.5 mL LB cultures containing 100 ug/mL kanamycin and 1% (w/v) glucose in a deep-well 96-well plate and grown overnight in a shaking incubator at 30°C.

    Article Title: Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin.
    Article Snippet: For transformation, 5 µL (200 ng – 2 µg) of plasmid DNA was added to 50 µL of the electrocompetent cells, transferred to a chilled 0.1 cm electroporation cuvette, and electroporated at 1.6 kV, 25 uF, 200 ohms. .. 450 µL SOC outgrowth medium (NEB) was added to the cells immediately after electroporation and the resuspended cells were incubated shaking at 225 rpm, 30°C, for one hour. .. T transformation was plated on an LB agar plate appropriate antibiotics (30 µg/mL tetracycline for pCM433 based plasmids, 50 µg/mL kanamycin for pK18mobsacB based plasmids) and incubated at 30°C overnight.

    In Vitro:

    Article Title: Enabling Biocatalysis by High-Throughput Protein Engineering Using Droplet Microfluidics Coupled to Mass Spectrometry
    Article Snippet: .. PURExpress In Vitro Protein Synthesis Kit, RNAse inhibitor, and SOC outgrowth medium were from New England BioLabs (Ipswich, MA). ..

    Incubation:

    Article Title: Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin.
    Article Snippet: For transformation, 5 µL (200 ng – 2 µg) of plasmid DNA was added to 50 µL of the electrocompetent cells, transferred to a chilled 0.1 cm electroporation cuvette, and electroporated at 1.6 kV, 25 uF, 200 ohms. .. 450 µL SOC outgrowth medium (NEB) was added to the cells immediately after electroporation and the resuspended cells were incubated shaking at 225 rpm, 30°C, for one hour. .. T transformation was plated on an LB agar plate appropriate antibiotics (30 µg/mL tetracycline for pCM433 based plasmids, 50 µg/mL kanamycin for pK18mobsacB based plasmids) and incubated at 30°C overnight.

    Expressing:

    Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
    Article Snippet: .. Rosetta™ 2(DE3)pLysS Singles Competent Cells (Millipore cat. no. 71401–3) TwinStrep-SUMO-LwaCas13a expression plasmid (Addgene: pC013 - Twinstrep-SUMO-huLwCas13a, Plasmid # 90097) SOC Outgrowth Medium 25 mL (New England BioLabs cat. no. B9020S) Luria Broth (LB), Ready-Made Powder 250 g, (Thermo Scientific cat. no. J75854.30 TB Broth powder (VWR™ cat. no. 89126–196) Glycerol ≥99.5% (Sigma-Aldrich cat. no. G9012–1GA) Ampicillin, sodium salt (AmericanBio, cat. no. AB00115–00025; CAS 69–52-3) Isopropyl β-D-1-thiogalactopyranoside (IPTG), Sigma-Aldrich cat. no. I6758 Sodium chloride (Sigma-Aldrich cat. no. S7653) DL-Dithiothreitol solution, BioUltra, for molecular biology, 1 M in H2O (Sigma Aldrich cat. no. 43816–50ML) Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride solution 0.5M, pH 7.0 (Sigma-Aldrich cat. no. 646547–10X1ML) β-Mercaptoethanol (Sigma-Aldrich cat. no. M6250–100ML) CAUTION: β-Mercaptoethanol is considered toxic. ..

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  • 99
    New England Biolabs soc outgrowth medium
    Soc Outgrowth Medium, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soc outgrowth medium/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soc outgrowth medium - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

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