Journal: Nucleic Acids Research

Article Title: Efficient quantitative monitoring of translational initiation by RelE cleavage

doi: 10.1093/nar/gkac614

Figure Lengend Snippet: RelE captures differential translation initiation in eukaryotic systems. ( A ) Schematic of RelE Cleavage Assay for Eukaryotic 5′-UTR Isoforms. 5′ 32 P labeled cap structure is shown in yellow. Site of RelE cleavage is shown in red. Downstream sequence is shown in grey. ( B ) Representation of the various yeast 5′-UTR isoforms tested. ( C ) RelE cleavage of yeast 5′-UTR isoforms. Two-tailed unpaired t-test with Welch's correlation performed within isoform pairs ( n = 3, ** P ≤ 0.01).

Article Snippet: For yeast 5′-UTR isoforms, RNA was capped with 32 P-α-GTP via the Vaccinia Capping System (NEB).

Techniques: Cleavage Assay, Labeling, Sequencing, Two Tailed Test

Journal: Cell reports

Article Title: Mobilization of vitamin B 12 transporters alters competitive dynamics in a human gut microbe

doi: 10.1016/j.celrep.2021.110164

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Yeast Chromosomal PFG-Marker Mid-Range ladder , New England BioLabs , Cat. #N0345.

Techniques: DNA Sequencing, Recombinant, Software