taq  (New England Biolabs)


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  • 99
    Name:
    Standard Taq Reaction Buffer Pack
    Description:
    Standard Taq Reaction Buffer Pack 6 0 ml
    Catalog Number:
    b9014s
    Price:
    22
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs taq
    Standard Taq Reaction Buffer Pack
    Standard Taq Reaction Buffer Pack 6 0 ml
    https://www.bioz.com/result/taq/product/New England Biolabs
    Average 99 stars, based on 522 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia"

    Article Title: A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0000436

    SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.
    Figure Legend Snippet: SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.

    Techniques Used: Polymerase Chain Reaction, Amplification, Mutagenesis, Nucleic Acid Electrophoresis, Sequencing

    2) Product Images from "A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia"

    Article Title: A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0000436

    SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.
    Figure Legend Snippet: SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.

    Techniques Used: Polymerase Chain Reaction, Amplification, Mutagenesis, Nucleic Acid Electrophoresis, Sequencing

    Related Articles

    Amplification:

    Article Title: Reprograming the replisome of a semi-synthetic organism for the expansion of the genetic alphabet
    Article Snippet: .. Therefore, the overlap assembly PCR of the UBP containing amplicon and kanamycin resistance gene amplicon was performed on large-scale (2 mL of reaction mixture split into 40 individual 50-μL reactions) with the following solution conditions: UBP-containing amplicon (0.02 ng/μL), kanamycin resistance gene amplicon (0.02 ng/μL), primers (1 μM, ), d TPT3 TP (5 μM), d NaM TP (100 μM), dNTPs (200 μM), MgSO4 (1.2 mM), One Taq DNA Polymerase (0.025 U/μL), and One Taq Standard Reaction Buffer (1×). ..

    Article Title: Early Control of Mycobacterium tuberculosis Infection Requires il12rb1 Expression by rag1-Dependent Lineages
    Article Snippet: .. To screen for the presence of a wild-type il12rb1 allele, genomic DNA (gDNA) from tail snips was isolated using the Promega Wizard SV Genomic DNA method (Promega, Madison, WI) and amplified with primers NF1 (5′-CAGAGATCCTCCTGCCTCTG-3′) and NF2 (5′-TATGGTTCGGAGGGACAAAG-3′) in a 50-μl reaction mixture comprising 2 μl of gDNA, 1.5× NEB Taq buffer (from a 10× solution), 2 mM MgCl2 , 0.2 μM primer NF1, 0.2 μM primer NF2, 0.2 mM each deoxynucleoside triphosphate (dNTP), 5% dimethyl sulfoxide (DMSO), and 1U of Taq polymerase (New England BioLabs, Ipswich, MA). .. To screen for the knockout (KO) il12rb1 allele, gDNA was amplified with primers NF1 (sequence above) and NF3 (5′-TGGATGTGGAATGTGTGCGAG-3′) in a 50-μl reaction mixture comprising 2 μl of gDNA, 1.0× NEB Taq buffer (from a 10× solution), 2 mM MgCl2 , 0.2 μM primer NF1, 0.2 μM primer NF3, 0.2 mM each dNTP, 5% DMSO, and 1U of Taq polymerase.

    Isolation:

    Article Title: Early Control of Mycobacterium tuberculosis Infection Requires il12rb1 Expression by rag1-Dependent Lineages
    Article Snippet: .. To screen for the presence of a wild-type il12rb1 allele, genomic DNA (gDNA) from tail snips was isolated using the Promega Wizard SV Genomic DNA method (Promega, Madison, WI) and amplified with primers NF1 (5′-CAGAGATCCTCCTGCCTCTG-3′) and NF2 (5′-TATGGTTCGGAGGGACAAAG-3′) in a 50-μl reaction mixture comprising 2 μl of gDNA, 1.5× NEB Taq buffer (from a 10× solution), 2 mM MgCl2 , 0.2 μM primer NF1, 0.2 μM primer NF2, 0.2 mM each deoxynucleoside triphosphate (dNTP), 5% dimethyl sulfoxide (DMSO), and 1U of Taq polymerase (New England BioLabs, Ipswich, MA). .. To screen for the knockout (KO) il12rb1 allele, gDNA was amplified with primers NF1 (sequence above) and NF3 (5′-TGGATGTGGAATGTGTGCGAG-3′) in a 50-μl reaction mixture comprising 2 μl of gDNA, 1.0× NEB Taq buffer (from a 10× solution), 2 mM MgCl2 , 0.2 μM primer NF1, 0.2 μM primer NF3, 0.2 mM each dNTP, 5% DMSO, and 1U of Taq polymerase.

    Incubation:

    Article Title: R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation
    Article Snippet: .. DNA polymerase activity assay DNA substrates (Table ) were incubated with 20 μl of reaction buffer A (50 mM Tris-HCl [pH 8.0], 10 mM MgCl2, 2 mM DTT, 20 mM NaCl, 10% glycerol), 50 μM of each dATP, dGTP, and dTTP (NEB), 8 μM [α-32 P]-dCTP, and various amounts (0-20 ng) of WT or R152C recombinant Pol β for 30 mins at 37°C. .. The reaction was then stopped by adding equal volumes of gel loading buffer (90% formamide dye, 3 M EDTA, 0.02% bromophenol blue and 0.02% xylene cyanol), heated (5min, 95°C), separated by 15% PAGE containing 8 M urea, and visualized by autoradiography.

    Activity Assay:

    Article Title: R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation
    Article Snippet: .. DNA polymerase activity assay DNA substrates (Table ) were incubated with 20 μl of reaction buffer A (50 mM Tris-HCl [pH 8.0], 10 mM MgCl2, 2 mM DTT, 20 mM NaCl, 10% glycerol), 50 μM of each dATP, dGTP, and dTTP (NEB), 8 μM [α-32 P]-dCTP, and various amounts (0-20 ng) of WT or R152C recombinant Pol β for 30 mins at 37°C. .. The reaction was then stopped by adding equal volumes of gel loading buffer (90% formamide dye, 3 M EDTA, 0.02% bromophenol blue and 0.02% xylene cyanol), heated (5min, 95°C), separated by 15% PAGE containing 8 M urea, and visualized by autoradiography.

    Polymerase Chain Reaction:

    Article Title: ABCB1 haplotype and OPRM1 118A > G genotype interaction in methadone maintenance treatment pharmacogenetics
    Article Snippet: .. PCR reactions were performed in 30 μL total volume containing 100 ng DNA, 50 μM dNTPs, 0.1 μM each primer, 2.5 U Taq DNA polymerase and 1 × reaction buffer (New England Biolabs Inc, distributed by Genesearch Pty Ltd, Arundel, Australia). .. PCR cycling conditions were: 5 minutes at 94°C; 45 cycles of 30 seconds at 94°C, 30 seconds at 60°C, and 1.5 minutes at 72°C; and 5 minutes at 72°C.

    Article Title: A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia
    Article Snippet: .. Fifteen µL of the fluorescently-labeled PCR products, which contained ∼200–300 ng of DNA, were directly digested by 10 U of Taq I RE, 1× Taq I RE buffer and 1× Bovine Serum Albumin (all from New England Biolabs) overnight in a 25 µL reaction volume. ..

    Article Title: High intralocus variability and interlocus recombination promote immunological diversity in a minimal major histocompatibility system
    Article Snippet: .. PCR reactions for ACTB were performed in 20 μl volumes containing 0.5U Taq (New England Biolabs), 1× NEB reaction buffer, 0.75 μM MgCl2 , 0.2 mM dNTPs, 0.2 μM primers, and 1.0 μl DNA, with the following PCR cycling conditions: 95°C for 1:30, then 30 cycles of 95°C for 0:30, 53°C for 0:30, 68°C for 1:00, followed by 68°C for 5:00. .. MH amplifications were performed in 25 μl volumes containing 1U Taq (NEB), 1× NEB reaction buffer, 1.0 μM MgCl2 , 0.4 mM dNTPs, 0.2 μM primers and 2.5 μl DNA, with the following cycling parameters: 92°C for 0:10, then 40 cycles of 92°C for 0:10, 55°C for 0:30, 68°C for 2:00 (for MH IIα) or 92°C for 5:00, then 40 cycles of 92°C for 0:30, 62°C for 0:30, 68°C for 4:00, followed by 68°C for 15:00 (for MH IIβ).

    Polymerase Cycling Assembly:

    Article Title: Reprograming the replisome of a semi-synthetic organism for the expansion of the genetic alphabet
    Article Snippet: .. Therefore, the overlap assembly PCR of the UBP containing amplicon and kanamycin resistance gene amplicon was performed on large-scale (2 mL of reaction mixture split into 40 individual 50-μL reactions) with the following solution conditions: UBP-containing amplicon (0.02 ng/μL), kanamycin resistance gene amplicon (0.02 ng/μL), primers (1 μM, ), d TPT3 TP (5 μM), d NaM TP (100 μM), dNTPs (200 μM), MgSO4 (1.2 mM), One Taq DNA Polymerase (0.025 U/μL), and One Taq Standard Reaction Buffer (1×). ..

    Recombinant:

    Article Title: R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation
    Article Snippet: .. DNA polymerase activity assay DNA substrates (Table ) were incubated with 20 μl of reaction buffer A (50 mM Tris-HCl [pH 8.0], 10 mM MgCl2, 2 mM DTT, 20 mM NaCl, 10% glycerol), 50 μM of each dATP, dGTP, and dTTP (NEB), 8 μM [α-32 P]-dCTP, and various amounts (0-20 ng) of WT or R152C recombinant Pol β for 30 mins at 37°C. .. The reaction was then stopped by adding equal volumes of gel loading buffer (90% formamide dye, 3 M EDTA, 0.02% bromophenol blue and 0.02% xylene cyanol), heated (5min, 95°C), separated by 15% PAGE containing 8 M urea, and visualized by autoradiography.

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    New England Biolabs taq
    SDHB mutations in genomic DNAs (gDNAs). A. <t>Taq</t> I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type <t>DNA</t> co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.
    Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq/product/New England Biolabs
    Average 99 stars, based on 259 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.

    Journal: PLoS ONE

    Article Title: A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia

    doi: 10.1371/journal.pone.0000436

    Figure Lengend Snippet: SDHB mutations in genomic DNAs (gDNAs). A. Taq I RE digestion of PCR-amplified exon 2 shows no evidence of undigested mutant DNAs at 260 bp size (pointed by an arrow head). Taq I RE digestion products of the wild-type DNA co-migrate at sizes of 128 and 132 bps (denoted by a star). B. Gel electrophoresis of Taq I RE-digested (mutation) enrichment PCR ( e PCR) products shows variable amounts of mutant gDNAs at 233 bp size (pointed by an arrow head), which are confirmed to be primarily composed of c.136C > T by sequencing ( Table 1 ). Taq I digestion of wild-type e PCR products gives two bands at sizes, 101 and 132 bp (shown by a star). C. e PCR analyses of leukemic cell lines demonstrate the presence of mutant gDNA in the MOLT-4 cell line (pointed by an arrow head) but not in other leukemic cell lines in this experiment. D. Direct sequence analysis of a gDNA e PCR product shows selective enrichment of the R46X mutation. E, F. Sequence chromatograms of the heterozygous DNA mutations detected in two T-ALL cell lines are shown.

    Article Snippet: Fifteen µL of the fluorescently-labeled PCR products, which contained ∼200–300 ng of DNA, were directly digested by 10 U of Taq I RE, 1× Taq I RE buffer and 1× Bovine Serum Albumin (all from New England Biolabs) overnight in a 25 µL reaction volume.

    Techniques: Polymerase Chain Reaction, Amplification, Mutagenesis, Nucleic Acid Electrophoresis, Sequencing