nicotinamide adenine dinucleotide nad  (New England Biolabs)


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    Name:
    beta Nicotinamide adenine dinucleotide NAD
    Description:
    beta Nicotinamide adenine dinucleotide NAD 0 2 ml
    Catalog Number:
    b9007s
    Price:
    33
    Size:
    0 2 ml
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    Buffers
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    New England Biolabs nicotinamide adenine dinucleotide nad
    beta Nicotinamide adenine dinucleotide NAD
    beta Nicotinamide adenine dinucleotide NAD 0 2 ml
    https://www.bioz.com/result/nicotinamide adenine dinucleotide nad/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nicotinamide adenine dinucleotide nad - by Bioz Stars, 2020-11
    99/100 stars

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    1) Product Images from "Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions"

    Article Title: Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw998

    ATP-hydrolysis by RecA under the conditions for the ligation enhancement. Radioactive [α- 33 P]ATP (1.3 mM) was incubated with the indicated amounts of RecA for 30 min with RecA in the complete system, except for the amounts of DNA and the absence of DNA ligase and NAD. After the reaction, the amounts of hydrolyzed ATP were analyzed. The averages of more than three independent experiments were plotted with standard deviations. The small extent of ATP-hydrolysis observed in the absence of DNA was probably caused by an impurity in the RecA preparations used in these experiments, since the values varied from 0 to a few nmol among RecA preparations. Closed symbols, reactions in the basic reaction mixture (with 3.8% (w/v) PEG), open symbols, omit PEG. •, ∘ (circles), linear dsDNA with 5′ four nucleotide overhangs generated by Hin dIII; ▪, □ (squares), linear dsDNA with 3′ four nucleotide overhangs generated by Pst I; ▴, Δ (triangles), linear dsDNA with blunt ends generated by Hin cII; ♦, ◊ (diamonds), ssDNA; ▿ (reversed triangles), ssDNA without PEG and in the presence of 13 mM MgCl 2 (the standard reaction mixtures for homologous joint formation by RecA: ( 31 )); ▹ (sideways triangles), without DNA (with 1.3 mM MgCl 2 , without PEG).
    Figure Legend Snippet: ATP-hydrolysis by RecA under the conditions for the ligation enhancement. Radioactive [α- 33 P]ATP (1.3 mM) was incubated with the indicated amounts of RecA for 30 min with RecA in the complete system, except for the amounts of DNA and the absence of DNA ligase and NAD. After the reaction, the amounts of hydrolyzed ATP were analyzed. The averages of more than three independent experiments were plotted with standard deviations. The small extent of ATP-hydrolysis observed in the absence of DNA was probably caused by an impurity in the RecA preparations used in these experiments, since the values varied from 0 to a few nmol among RecA preparations. Closed symbols, reactions in the basic reaction mixture (with 3.8% (w/v) PEG), open symbols, omit PEG. •, ∘ (circles), linear dsDNA with 5′ four nucleotide overhangs generated by Hin dIII; ▪, □ (squares), linear dsDNA with 3′ four nucleotide overhangs generated by Pst I; ▴, Δ (triangles), linear dsDNA with blunt ends generated by Hin cII; ♦, ◊ (diamonds), ssDNA; ▿ (reversed triangles), ssDNA without PEG and in the presence of 13 mM MgCl 2 (the standard reaction mixtures for homologous joint formation by RecA: ( 31 )); ▹ (sideways triangles), without DNA (with 1.3 mM MgCl 2 , without PEG).

    Techniques Used: Ligation, Incubation, Generated

    Related Articles

    Ligation:

    Article Title: Transposable element expression at unique loci in single cells with CELLO-seq
    Article Snippet: .. The splint ligation reaction was performed by preparing a 5 µl mastermix that consisted of 0.5 µL Hifi Taq Ligase (NEB #M0647S), 0.5 µl 10x Taq ligase buffer, 1µl of 10 µM annealed splint oligo, 0.25 µL NAD+ (NEB #B9007S) and 2.75 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). ..

    Purification:

    Article Title: Genetically controlled membrane synthesis in liposomes
    Article Snippet: .. Purified PCR products were mixed following the pipetting scheme in Supplementary Table plus 15 µL of prepared Gibson assembly mix containing 100 mM Tris-HCl, 50 mM MgCl2 , 0.2 mM each dNTP, 10 mM dithiothreitol (DTT), 5% w/v PEG-8000, 1 mM nicotinamide adenine dinucleotide (NAD), 5.33 U mL–1 T5 Exonuclease, 33.3 U mL–1 Phusion polymerase and 5.33 U mL–1 Taq-ligase in a final volume of 20 µL. .. The Gibson assembly mixture was incubated at 50 °C for 1 h and 5 µL were subsequently used for transformation of 50 µL One Shot™ TOP10 Chemically Competent E. coli cells (ThermoFisher Scientific, USA, catalogue number C4040-10).

    Concentration Assay:

    Article Title: High-Throughput Generation of In Silico Derived Synthetic Antibodies via One-step Enzymatic DNA Assembly of Fragments.
    Article Snippet: .. Phage-display technology offers robust methods for isolating antibody (Ab) molecules with specificity for different target antigens. .. Phage-display technology offers robust methods for isolating antibody (Ab) molecules with specificity for different target antigens.

    Incubation:

    Article Title: A Serine-Threonine Kinase (StkP) Regulates Expression of the Pneumococcal Pilus and Modulates Bacterial Adherence to Human Epithelial and Endothelial Cells In Vitro
    Article Snippet: .. Finally 2μl of 1mM NAD+ (β-Nicotinamide adenine dinucleotide, NEB) and 4μl E .coli DNA ligase (NEB) was added, incubated at 16°C overnight and then stored at 4°C until transformation. ..

    other:

    Article Title: Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions
    Article Snippet: Basic reaction mixture The basic reaction mixture contained 30 mM Tris-HCl (pH 7.5), 7 mM MgCl2 , 3.8% (w/v) polyethylene glycol 6000 (PEG), 0.1 mM nicotinamide adenine dinucleotide (NAD), 0.1 mM ethylenediaminetetraacetic acid (EDTA), 1.8 mM dithiothreitol (DTT) and 88 μg/ml bovine serum albumin ((BSA); New England Biolabs and Roche, molecular biology grade).

    Polymerase Chain Reaction:

    Article Title: Genetically controlled membrane synthesis in liposomes
    Article Snippet: .. Purified PCR products were mixed following the pipetting scheme in Supplementary Table plus 15 µL of prepared Gibson assembly mix containing 100 mM Tris-HCl, 50 mM MgCl2 , 0.2 mM each dNTP, 10 mM dithiothreitol (DTT), 5% w/v PEG-8000, 1 mM nicotinamide adenine dinucleotide (NAD), 5.33 U mL–1 T5 Exonuclease, 33.3 U mL–1 Phusion polymerase and 5.33 U mL–1 Taq-ligase in a final volume of 20 µL. .. The Gibson assembly mixture was incubated at 50 °C for 1 h and 5 µL were subsequently used for transformation of 50 µL One Shot™ TOP10 Chemically Competent E. coli cells (ThermoFisher Scientific, USA, catalogue number C4040-10).

    Article Title: Genetically controlled membrane synthesis in liposomes
    Article Snippet: .. 100 ng of backbone and an equimolar amount of the egfp-lactC2 PCR fragment were mixed in a solution containing 100 mM Tris-HCl, 50 mM MgCl2 , 0.2 mM each dNTP, 10 mM dithiothreitol (DTT), 5% w/v PEG-8000, 1 mM nicotinamide adenine dinucleotide, 5.33 U mL−1 T5 Exonuclease, 33.3 U mL−1 Phusion polymerase and 5.33 U mL−1 Taq-ligase in a final volume of 20 µL. .. The assembly reaction was incubated at 50 °C for 60 min. Then, 20 U µL−1 of DpnI restriction enzyme (New England Biolabs, USA) were added to digest possible methylated DNA left and the mixture was incubated for an additional 15 min at 37 °C.

    Transformation Assay:

    Article Title: A Serine-Threonine Kinase (StkP) Regulates Expression of the Pneumococcal Pilus and Modulates Bacterial Adherence to Human Epithelial and Endothelial Cells In Vitro
    Article Snippet: .. Finally 2μl of 1mM NAD+ (β-Nicotinamide adenine dinucleotide, NEB) and 4μl E .coli DNA ligase (NEB) was added, incubated at 16°C overnight and then stored at 4°C until transformation. ..

    Crocin Bleaching Assay:

    Article Title: Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions
    Article Snippet: .. To make the linear cassette, 25 fmoles of each fragment were combined in a 20 ul isothermal assembly reaction with 2× CBA isothermal assembly mastermix (320 μl of 5 × ISO Reaction Buffer [25% polyethylene glycol 8000, 500 mM tris-HCl (pH 8.0), 50 mM MgCl2 , 50 mM dithiothreitol (DTT), 1.0 μM each dNTPs, 5 μM nicotinamide adenine dinucleotide, 6.4 μl of T5 Exonuclease (1 U/ml) (Epicentre), 20 μl of Phusion polymerase (2 U/ml) [New England Biolabs (NEB)], 80 μl of Taq ligase (40 U/ml) (NEB), and 374 μl of deionized H2 O . .. The cassette was amplified using 1ul of the isothermal assembly reaction, with primers 15165 and 15169 (0.4 μM) in a PCR reaction with 2× Phusion HF mastermix, (100 ul, 25 cycle, 60 °C melting temperature, 6 minute extension) and purified using the Qiagen Qiaquick PCR purification kit.

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    New England Biolabs nicotinamide adenine dinucleotide nad
    ATP-hydrolysis by RecA under the conditions for the ligation enhancement. Radioactive [α- 33 P]ATP (1.3 mM) was incubated with the indicated amounts of RecA for 30 min with RecA in the complete system, except for the amounts of DNA and the absence of DNA ligase and <t>NAD.</t> After the reaction, the amounts of hydrolyzed ATP were analyzed. The averages of more than three independent experiments were plotted with standard deviations. The small extent of ATP-hydrolysis observed in the absence of DNA was probably caused by an impurity in the RecA preparations used in these experiments, since the values varied from 0 to a few nmol among RecA preparations. Closed symbols, reactions in the basic reaction mixture (with 3.8% (w/v) <t>PEG),</t> open symbols, omit PEG. •, ∘ (circles), linear dsDNA with 5′ four nucleotide overhangs generated by Hin dIII; ▪, □ (squares), linear dsDNA with 3′ four nucleotide overhangs generated by Pst I; ▴, Δ (triangles), linear dsDNA with blunt ends generated by Hin cII; ♦, ◊ (diamonds), ssDNA; ▿ (reversed triangles), ssDNA without PEG and in the presence of 13 mM MgCl 2 (the standard reaction mixtures for homologous joint formation by RecA: ( 31 )); ▹ (sideways triangles), without DNA (with 1.3 mM MgCl 2 , without PEG).
    Nicotinamide Adenine Dinucleotide Nad, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nicotinamide adenine dinucleotide nad/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nicotinamide adenine dinucleotide nad - by Bioz Stars, 2020-11
    99/100 stars
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    ATP-hydrolysis by RecA under the conditions for the ligation enhancement. Radioactive [α- 33 P]ATP (1.3 mM) was incubated with the indicated amounts of RecA for 30 min with RecA in the complete system, except for the amounts of DNA and the absence of DNA ligase and NAD. After the reaction, the amounts of hydrolyzed ATP were analyzed. The averages of more than three independent experiments were plotted with standard deviations. The small extent of ATP-hydrolysis observed in the absence of DNA was probably caused by an impurity in the RecA preparations used in these experiments, since the values varied from 0 to a few nmol among RecA preparations. Closed symbols, reactions in the basic reaction mixture (with 3.8% (w/v) PEG), open symbols, omit PEG. •, ∘ (circles), linear dsDNA with 5′ four nucleotide overhangs generated by Hin dIII; ▪, □ (squares), linear dsDNA with 3′ four nucleotide overhangs generated by Pst I; ▴, Δ (triangles), linear dsDNA with blunt ends generated by Hin cII; ♦, ◊ (diamonds), ssDNA; ▿ (reversed triangles), ssDNA without PEG and in the presence of 13 mM MgCl 2 (the standard reaction mixtures for homologous joint formation by RecA: ( 31 )); ▹ (sideways triangles), without DNA (with 1.3 mM MgCl 2 , without PEG).

    Journal: Nucleic Acids Research

    Article Title: Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions

    doi: 10.1093/nar/gkw998

    Figure Lengend Snippet: ATP-hydrolysis by RecA under the conditions for the ligation enhancement. Radioactive [α- 33 P]ATP (1.3 mM) was incubated with the indicated amounts of RecA for 30 min with RecA in the complete system, except for the amounts of DNA and the absence of DNA ligase and NAD. After the reaction, the amounts of hydrolyzed ATP were analyzed. The averages of more than three independent experiments were plotted with standard deviations. The small extent of ATP-hydrolysis observed in the absence of DNA was probably caused by an impurity in the RecA preparations used in these experiments, since the values varied from 0 to a few nmol among RecA preparations. Closed symbols, reactions in the basic reaction mixture (with 3.8% (w/v) PEG), open symbols, omit PEG. •, ∘ (circles), linear dsDNA with 5′ four nucleotide overhangs generated by Hin dIII; ▪, □ (squares), linear dsDNA with 3′ four nucleotide overhangs generated by Pst I; ▴, Δ (triangles), linear dsDNA with blunt ends generated by Hin cII; ♦, ◊ (diamonds), ssDNA; ▿ (reversed triangles), ssDNA without PEG and in the presence of 13 mM MgCl 2 (the standard reaction mixtures for homologous joint formation by RecA: ( 31 )); ▹ (sideways triangles), without DNA (with 1.3 mM MgCl 2 , without PEG).

    Article Snippet: Basic reaction mixture The basic reaction mixture contained 30 mM Tris-HCl (pH 7.5), 7 mM MgCl2 , 3.8% (w/v) polyethylene glycol 6000 (PEG), 0.1 mM nicotinamide adenine dinucleotide (NAD), 0.1 mM ethylenediaminetetraacetic acid (EDTA), 1.8 mM dithiothreitol (DTT) and 88 μg/ml bovine serum albumin ((BSA); New England Biolabs and Roche, molecular biology grade).

    Techniques: Ligation, Incubation, Generated