thermopol ii reaction buffer  (New England Biolabs)


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    Name:
    ThermoPol II Mg free Reaction Buffer Pack
    Description:
    ThermoPol II Mg free Reaction Buffer Pack 6 0 ml
    Catalog Number:
    b9005s
    Price:
    22
    Size:
    6 0 ml
    Category:
    DNA Analysis
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    New England Biolabs thermopol ii reaction buffer
    ThermoPol II Mg free Reaction Buffer Pack
    ThermoPol II Mg free Reaction Buffer Pack 6 0 ml
    https://www.bioz.com/result/thermopol ii reaction buffer/product/New England Biolabs
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    thermopol ii reaction buffer - by Bioz Stars, 2020-10
    93/100 stars

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    Polymerase Chain Reaction:

    Article Title: Optogenetic Control of Motor Coordination by Gi/o Protein-coupled Vertebrate Rhodopsin in Cerebellar Purkinje Cells *
    Article Snippet: .. The 50-ml final PCR reaction contained 1 ml of gDNA, 1 ml of each primer, 1 ml of dNTP mix (10 m m each of dATP, dTTP, dCTP, dGTP; New England Biolabs (NEB)), 5 ml of 10× Thermopol II reaction buffer (NEB), 5 ml of dimethyl sulfoxide, 0.5 ml of Taq Polymerase (NEB), and 35.5 ml of distilled H2 O. PCR reactions were run on an Eppendorf thermocycler using the following conditions: 92 °C for 30 s, 60 °C for 45 s, and 72 °C for 1 min run for 40 cycles or 95 °C for 30 s, 55 °C for 1 min and 72 °C for 1 min 30 s for 40 cycles to detect vRh-GFP or Cre-Recombinase, respectively. ..

    Article Title: Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae)
    Article Snippet: .. PCR was performed in 25 μL volumes using: Taq DNA polymerase (0.2 U) (New England Biolabs); 1 × Thermopol buffer or Thermopol II buffer (New England Biolabs); an equimolar mix of 0.2 mM dNTPs (Fisher Biotech); 0.4 μM each primer; and 5 μg acetylated bovine serum albumin. ..

    Article Title: Osteosarcoma is characterised by reduced expression of markers of osteoclastogenesis and antigen presentation compared with normal bone
    Article Snippet: .. For PCR, 2 μ g of non-malignant bone or OS tumour biopsy RNA was reverse transcribed with BioScript (Bioline, Sydney, Australia); PCR was performed using Taq DNA polymerase with ThermoPol II buffer (New England Biolabs, Ipswich, MA, USA) at an annealing temperature of 53–55°C for 30 cycles on a ThermoHybaid PxE0.2 (Thermo Scientific, Waltham, MA, USA). ..

    Article Title: 5-Aza-2?-deoxycytidine Sensitizes Busulfan-resistant Myeloid Leukemia Cells By Regulating Expression of Genes Involved in Cell Cycle Checkpoint and Apoptosis
    Article Snippet: .. Methylation-specific PCR (MSP) was performed using 30 ng bisulfite-modified DNA, 0.5 μM of each primer , 1 mM each of dATP, dCTP, dGTP and dTTP, 5 mM MgSO4 , and Taq DNA polymerase with ThermoPol II buffer (New England Biolabs, Ipswich, MA). .. The amplification method included initial heating at 95°C for 3 min, followed by 30 cycles of 95°C for 30 s, 57°C or 65°C for 30 s depending on the primers (see ), and 72°C for 33 s, followed by extension at 72°C for 10 min. MSP products were visualized on a 2.5% agarose gel and DNA band intensities were analyzed using Quant One software (Bio-Rad, Hercules, CA).

    Methylation:

    Article Title: 5-Aza-2?-deoxycytidine Sensitizes Busulfan-resistant Myeloid Leukemia Cells By Regulating Expression of Genes Involved in Cell Cycle Checkpoint and Apoptosis
    Article Snippet: .. Methylation-specific PCR (MSP) was performed using 30 ng bisulfite-modified DNA, 0.5 μM of each primer , 1 mM each of dATP, dCTP, dGTP and dTTP, 5 mM MgSO4 , and Taq DNA polymerase with ThermoPol II buffer (New England Biolabs, Ipswich, MA). .. The amplification method included initial heating at 95°C for 3 min, followed by 30 cycles of 95°C for 30 s, 57°C or 65°C for 30 s depending on the primers (see ), and 72°C for 33 s, followed by extension at 72°C for 10 min. MSP products were visualized on a 2.5% agarose gel and DNA band intensities were analyzed using Quant One software (Bio-Rad, Hercules, CA).

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  • 93
    New England Biolabs thermopol ii reaction buffer
    Thermopol Ii Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermopol ii reaction buffer/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    thermopol ii reaction buffer - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

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