diluent a  (New England Biolabs)


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  • 94
    Name:
    Diluent A
    Description:
    Diluent A 5 0 ml
    Catalog Number:
    b8001s
    Price:
    33
    Size:
    5 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs diluent a
    Diluent A
    Diluent A 5 0 ml
    https://www.bioz.com/result/diluent a/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    diluent a - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Article Title: Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
    Article Snippet: .. RpnA was purified from the resulting strain (ER3573) as described in the kit's manual and then concentrated with a Vivaspin20 centrifugal ultrafiltration device (molecular weight cutoff, 10,000; GE Healthcare Life Sciences), dialyzed overnight in diluent A (catalog number B8001S; NEB), and kept at −20°C. .. RpnA purified with the Impact system was further purified by anion-exchange chromatography.

    other:

    Article Title:
    Article Snippet: Diluent A (10 mm Tris-HCl, pH 7.4, at 25 °C, 1 mm DTT, 0.1 mm EDTA, 50% glycerol, 200 μg/ml BSA, 50 mm KCl) was obtained from New England Biolabs.

    Binding Assay:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Plasmid Preparation:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Purification:

    Article Title: Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
    Article Snippet: .. RpnA was purified from the resulting strain (ER3573) as described in the kit's manual and then concentrated with a Vivaspin20 centrifugal ultrafiltration device (molecular weight cutoff, 10,000; GE Healthcare Life Sciences), dialyzed overnight in diluent A (catalog number B8001S; NEB), and kept at −20°C. .. RpnA purified with the Impact system was further purified by anion-exchange chromatography.

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  • 94
    New England Biolabs diluent a
    Diluent A, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diluent a/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    diluent a - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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